BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,870,741	T→C	V286A (GTA→GCA)	yeaJ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,870,741	0	T	C	99.7%	1091.1 / ‑4.8	316	V286A (GTA→GCA)	yeaJ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (157/158); total (158/158)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

Only 100 of 325 total aligned reads displayed.

TGAAAAGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTC  >  NC_000913/1870652‑1870830

tGAAAAGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGcata                                                                                        <  2:6636488/93‑1 (MQ=255)

tGAAAAGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGcata                                                                                        >  1:4025820/1‑93 (MQ=255)

 gAAAAGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCatat                                                                                       >  1:2689874/1‑93 (MQ=255)

 gAAAAGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCatat                                                                                       <  2:1413114/93‑1 (MQ=255)

  aaaaGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATg                                                                                      <  2:7592205/93‑1 (MQ=255)

  aaaaGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATg                                                                                      >  1:703071/1‑93 (MQ=255)

  aaaaGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATg                                                                                      >  2:6992009/1‑93 (MQ=255)

   aaaGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGt                                                                                     <  1:1115354/93‑1 (MQ=255)

    aaGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTc                                                                                    >  2:4149818/1‑93 (MQ=255)

    aaGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTc                                                                                    <  1:1811780/93‑1 (MQ=255)

    aaGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTc                                                                                    <  1:7831017/93‑1 (MQ=255)

      ggTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCgt                                                                                  >  2:301349/1‑92 (MQ=255)

      ggTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGc                                                                                  <  2:497518/93‑1 (MQ=255)

       gTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCa                                                                                 >  2:6407681/1‑93 (MQ=255)

        tATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAg                                                                                >  1:8097471/1‑93 (MQ=255)

        tATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAg                                                                                >  2:5919087/1‑93 (MQ=255)

         aTTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAATGTGATGAGTTTGCTGACGCCGGCATATGTCGCAgg                                                                               >  2:6065772/1‑93 (MQ=255)

         aTTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAgg                                                                               <  2:6244986/93‑1 (MQ=255)

          tttACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAggg                                                                              <  1:2915496/93‑1 (MQ=255)

          tttACCGGGCGTACCGTATTGTCGAGCATTTACCAGCATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAggg                                                                              <  1:563342/93‑1 (MQ=255)

           ttACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGc                                                                             >  1:909421/1‑93 (MQ=255)

            tACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCa                                                                            >  1:7433549/1‑93 (MQ=255)

             tccGGGCGTACCATATTGTCGAGTATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAg                                                                           <  2:1595302/92‑1 (MQ=255)

             aCCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAg                                                                           >  2:6956888/1‑93 (MQ=255)

             aCCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAg                                                                           >  2:5592546/1‑93 (MQ=255)

                gggCGTACCGTATTGGCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTa                                                                        >  1:8586471/1‑93 (MQ=255)

                  gCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTaaa                                                                      <  1:2022422/93‑1 (MQ=255)

                   cGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTaaaa                                                                     <  2:6194576/93‑1 (MQ=255)

                   cGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTaaaa                                                                     <  1:351361/93‑1 (MQ=255)

                    gTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAg                                                                    >  2:8507786/1‑93 (MQ=255)

                     tACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAgg                                                                   <  1:2532521/93‑1 (MQ=255)

                      aCCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAggg                                                                  >  2:2450425/1‑93 (MQ=255)

                      aCCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAggg                                                                  <  2:7342589/93‑1 (MQ=255)

                      aCCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAggg                                                                  >  1:7211561/1‑93 (MQ=255)

                       ccGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGa                                                                 <  2:4709180/93‑1 (MQ=255)

                       ccGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGa                                                                 >  2:3303733/1‑93 (MQ=255)

                       ccGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGa                                                                 >  1:6650823/1‑93 (MQ=255)

                          tATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATtg                                                              <  1:2111972/93‑1 (MQ=255)

                           aTTGTCGAGCATTTAACAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATtgt                                                             <  1:3286662/93‑1 (MQ=255)

                                   gCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTgg                                                     <  2:5656635/93‑1 (MQ=255)

                                   gCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTgg                                                     >  1:3909003/1‑93 (MQ=255)

                                   gCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTgg                                                     <  1:2197045/93‑1 (MQ=255)

                                      tttACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGata                                                  >  1:151726/1‑93 (MQ=255)

                                        tACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATAtt                                                >  2:8599170/1‑93 (MQ=255)

                                        tACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATAtt                                                <  1:6046691/93‑1 (MQ=255)

                                         aCCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTa                                               <  2:5090450/93‑1 (MQ=255)

                                          ccAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTaa                                              <  2:6762024/93‑1 (MQ=255)

                                          ccAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTaa                                              <  1:5343774/93‑1 (MQ=255)

                                            aGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACa                                            <  2:1282262/93‑1 (MQ=255)

                                             ggATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACaa                                           <  2:4532028/93‑1 (MQ=255)

                                              gATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACaaa                                          >  1:5278318/1‑93 (MQ=255)

                                                tCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACaaaaa                                        >  1:6900883/1‑93 (MQ=255)

                                                tCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACaaaaa                                        <  1:5779489/93‑1 (MQ=255)

                                                 cAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAAc                                       >  2:6003603/1‑93 (MQ=255)

                                                   gCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACaa                                     <  1:7011093/93‑1 (MQ=255)

                                                   gCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACaa                                     <  2:1499871/93‑1 (MQ=255)

                                                    cGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAAt                                    >  1:3088520/1‑93 (MQ=255)

                                                     gTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATc                                   <  1:7343291/93‑1 (MQ=255)

                                                      tACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCt                                  >  1:5299381/1‑93 (MQ=255)

                                                      tACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCt                                  <  2:3391886/93‑1 (MQ=255)

                                                      tACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCt                                  <  1:4644443/93‑1 (MQ=255)

                                                      tACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCt                                  <  2:2775368/93‑1 (MQ=255)

                                                      tACTAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCt                                  <  1:1617349/93‑1 (MQ=255)

                                                         tAAACAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCg                               >  2:8072293/1‑93 (MQ=255)

                                                          aaaCAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCgg                              >  1:7564644/1‑93 (MQ=255)

                                                          aaaCAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCCAAAAGGGATTGTGCTGCTGGATAGTAACAAAAACAATCTGCgg                              >  2:7812346/1‑93 (MQ=255)

                                                           aaCAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGa                             >  1:2146848/1‑93 (MQ=255)

                                                            aCAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGaa                            <  1:6792974/93‑1 (MQ=255)

                                                            aCAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGaa                            >  1:5000215/1‑93 (MQ=255)

                                                            aCAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGaa                            <  1:4145116/93‑1 (MQ=255)

                                                             cAGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAat                           <  1:973474/93‑1 (MQ=255)

                                                              aGAACGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAata                          <  2:3705977/93‑1 (MQ=255)

                                                                 aCGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTGAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCt                       >  1:7696606/1‑93 (MQ=255)

                                                                 aCGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCt                       >  1:594005/1‑93 (MQ=255)

                                                                 aCGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCt                       >  1:4838582/1‑93 (MQ=255)

                                                                 aCGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCt                       <  2:8203775/93‑1 (MQ=255)

                                                                 aCGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCt                       >  1:87353/1‑93 (MQ=255)

                                                                 aCGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCt                       <  2:1897133/93‑1 (MQ=255)

                                                                 aCGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCt                       >  1:8404136/1‑93 (MQ=255)

                                                                  cGTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCtt                      >  1:8540348/1‑93 (MQ=255)

                                                                   gTGATGAGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCttt                     <  2:3671611/93‑1 (MQ=255)

                                                                    tgatgaGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCtttt                    >  2:4427581/1‑93 (MQ=255)

                                                                     gatgaGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTta                   <  2:5833275/93‑1 (MQ=255)

                                                                     gatgaGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTta                   >  2:2910613/1‑93 (MQ=255)

                                                                     gatgaGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTta                   >  1:3638723/1‑93 (MQ=255)

                                                                        gaGTTTGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATAc                <  2:6260308/93‑1 (MQ=255)

                                                                           tttGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCa             <  2:4215576/93‑1 (MQ=255)

                                                                           tttGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCa             >  2:7178552/1‑93 (MQ=255)

                                                                            ttGCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCAt            <  2:168537/93‑1 (MQ=255)

                                                                              gCTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGa          >  2:3818928/1‑93 (MQ=255)

                                                                               cTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGAc         <  1:513978/93‑1 (MQ=255)

                                                                               cTGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGAc         <  2:2172283/93‑1 (MQ=255)

                                                                                tGACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGAcc        >  1:2383107/1‑93 (MQ=255)

                                                                                 gACGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCg       >  1:440205/1‑93 (MQ=255)

                                                                                  gcGCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTAGATATTAACAAAAACAATCTGCGGAATATCTTTTATCCTCATGACCGc      <  2:1815751/92‑1 (MQ=255)

                                                                                    gCCGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGccc    <  1:6175791/93‑1 (MQ=255)

                                                                                     ccGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCcct   >  2:5329211/1‑93 (MQ=255)

                                                                                     ccGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCcct   >  1:6462453/1‑93 (MQ=255)

                                                                                     ccGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATACTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCcct   <  2:4776384/93‑1 (MQ=255)

                                                                                      cGGCATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCcctc  <  2:5453864/93‑1 (MQ=255)

TGAAAAGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTC  >  NC_000913/1870652‑1870830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`