BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,885,627	A→G	L130P (CTG→CCG)	rnd ←	RNase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,885,627	0	A	G	99.7%	1023.0 / ‑5.2	299	L130P (CTG→CCG)	rnd	RNase D
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (163/135); total (164/135)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

Only 100 of 311 total aligned reads displayed.

AACAGATACCAGACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATC  >  NC_000913/1885537‑1885716

 aCAGATACCAGACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCg                                                                                        >  2:488799/1‑93 (MQ=255)

 aCAGATACCAGACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCg                                                                                        <  2:1878025/93‑1 (MQ=255)

     atcccAGACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAAc                                                                                    <  2:535372/90‑1 (MQ=255)

     aTACCAGACAGCGGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAAc                                                                                    <  1:6228176/93‑1 (MQ=255)

     aTACCAGACACCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAAc                                                                                    <  1:6556771/93‑1 (MQ=255)

      tACCAGACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACg                                                                                   >  2:8783579/1‑93 (MQ=255)

      tACCAGACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACg                                                                                   >  1:2157534/1‑93 (MQ=255)

       aCCAGACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGc                                                                                  <  1:2859012/93‑1 (MQ=255)

        ccAGACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGcc                                                                                 >  1:7720276/1‑93 (MQ=255)

          aGACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCgg                                                                               >  2:5075018/1‑93 (MQ=255)

          aGACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCgg                                                                               >  2:1117292/1‑93 (MQ=255)

           gcagtcctccGCTGCGTCTTCACACTGACGTTCGGTCAGCGGTCGGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGa                                                                              <  2:5027387/89‑1 (MQ=255)

           gACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGa                                                                              >  2:124619/1‑93 (MQ=255)

            acacCCGCCGCTGCGTATTCACACTGACGTGCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACCCCGGaa                                                                             <  2:6261512/93‑1 (MQ=255)

             cATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAAt                                                                            >  2:7081223/1‑93 (MQ=255)

             cATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAAt                                                                            <  1:1639115/93‑1 (MQ=255)

              aTCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATa                                                                           >  2:3364122/1‑93 (MQ=255)

              aTCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATa                                                                           >  2:423280/1‑93 (MQ=255)

                ccgccgCTGCGTATTCACACTGACGTTCGGTCAGCGGCCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATAct                                                                         <  2:6448899/93‑1 (MQ=255)

                 cgccggTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATActc                                                                        <  1:8537114/93‑1 (MQ=255)

                 cgccgcTCCGTATTCACCCTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATActc                                                                        <  1:3404540/93‑1 (MQ=255)

                  gccgcTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATActct                                                                       >  1:8000493/1‑93 (MQ=255)

                  gccgcTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATActct                                                                       <  2:5262443/93‑1 (MQ=255)

                   ccgcTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCtt                                                                      >  1:595962/1‑93 (MQ=255)

                    cgcTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTc                                                                     <  2:4323512/93‑1 (MQ=255)

                    cgcTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTc                                                                     <  1:3689632/93‑1 (MQ=255)

                    cgcTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGAGTAACCGCGGAATACTCTTc                                                                     >  1:6389501/1‑93 (MQ=255)

                     gcTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTcc                                                                    <  2:7631421/93‑1 (MQ=255)

                      cTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTcca                                                                   <  1:7951977/93‑1 (MQ=255)

                       tGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTccac                                                                  >  1:7026501/1‑93 (MQ=255)

                       tGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTATTccac                                                                  >  2:7130114/1‑93 (MQ=255)

                         cGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTccacca                                                                >  1:5446906/1‑93 (MQ=255)

                         cGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTccacca                                                                <  2:267135/93‑1 (MQ=255)

                          gTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCAt                                                               <  2:6974291/93‑1 (MQ=255)

                             ttCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGa                                                            <  2:735071/93‑1 (MQ=255)

                              tCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGaa                                                           >  2:5923307/1‑93 (MQ=255)

                                ccacTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGc                                                         <  2:2357731/92‑1 (MQ=255)

                                 cacTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCg                                                        <  1:2974568/93‑1 (MQ=255)

                                  acTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGCGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGa                                                       >  1:733040/1‑93 (MQ=255)

                                    tGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGaaa                                                     <  2:4659576/93‑1 (MQ=255)

                                    tGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGaaa                                                     >  1:2569342/1‑93 (MQ=255)

                                     ggcGTTCGGCCCGCGGTCTGGCCAGCCGGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATCCTCTTCCACCATGGAAGCGAAAc                                                    <  1:3095136/91‑1 (MQ=255)

                                     gACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAAc                                                    <  1:1343522/93‑1 (MQ=255)

                                       cGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAAccc                                                  >  2:6807700/1‑93 (MQ=255)

                                        gTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAAcccc                                                 <  2:1018275/93‑1 (MQ=255)

                                         ttCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCa                                                >  2:5581340/1‑93 (MQ=255)

                                         ttCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCa                                                <  2:7671757/93‑1 (MQ=255)

                                         ttCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCa                                                <  1:6313528/93‑1 (MQ=255)

                                             gTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGAc                                            >  2:3623245/1‑93 (MQ=255)

                                              tCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACa                                           >  1:7218637/1‑93 (MQ=255)

                                                cgCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCGTGGAAGCGAAACCCCATGACATc                                         <  1:2027504/92‑1 (MQ=255)

                                                 gCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCg                                        >  1:1061757/1‑93 (MQ=255)

                                                 gCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCg                                        >  1:1743492/1‑93 (MQ=255)

                                                 gCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCg                                        <  1:1489546/93‑1 (MQ=255)

                                                   ggTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCggg                                      >  2:3043506/1‑93 (MQ=255)

                                                    gTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCgggg                                     >  1:747328/1‑92 (MQ=255)

                                                    gTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGc                                     >  2:3902716/1‑93 (MQ=255)

                                                    gTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGc                                     >  1:694203/1‑93 (MQ=255)

                                                    gTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGc                                     >  1:3775843/1‑93 (MQ=255)

                                                       tGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTc                                  >  2:6603628/1‑93 (MQ=255)

                                                       tGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTc                                  <  1:6784152/93‑1 (MQ=255)

                                                        ggCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTcc                                 <  1:5013328/93‑1 (MQ=255)

                                                        ggCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTcc                                 <  1:488799/93‑1 (MQ=255)

                                                         gccacccaGTCGATGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCg                                <  1:8710138/93‑1 (MQ=255)

                                                          ccagccagTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCACATGACATCGGGCGTCCGc                               >  2:1537214/1‑93 (MQ=255)

                                                           cagccagTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCa                              <  2:4610971/93‑1 (MQ=255)

                                                           cagccagTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCa                              <  1:6057944/93‑1 (MQ=255)

                                                            agccagTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAg                             >  2:615641/1‑93 (MQ=255)

                                                            agccagTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAg                             >  1:2937694/1‑93 (MQ=255)

                                                             gccagTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGa                            >  1:710269/1‑93 (MQ=255)

                                                              ccagTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGaa                           <  2:8016779/93‑1 (MQ=255)

                                                                 gTCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGgc                        <  2:859830/93‑1 (MQ=255)

                                                                  tCGGTGCGCGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGgca                       <  1:5785908/93‑1 (MQ=255)

                                                                   cGGTGCGCGATGCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGgcag                      <  2:4798258/93‑1 (MQ=255)

                                                                       gcgcGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAg                  <  2:7219432/93‑1 (MQ=255)

                                                                       gcgcGATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAg                  >  1:8771530/1‑93 (MQ=255)

                                                                        cgcgATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCACCAAgg                 >  2:3040643/1‑93 (MQ=255)

                                                                         gcgATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGa                >  1:4510664/1‑93 (MQ=255)

                                                                         gcgATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGa                >  1:7293327/1‑93 (MQ=255)

                                                                          cgATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGAt               >  2:8484815/1‑93 (MQ=255)

                                                                          cgATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGAt               <  2:7540074/93‑1 (MQ=255)

                                                                           gATTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGAtt              >  2:1339521/1‑93 (MQ=255)

                                                                            aTTCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGAttt             >  1:5552264/1‑93 (MQ=255)

                                                                             ttCACTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTg            >  2:5826255/1‑93 (MQ=255)

                                                                                aCTCTTGTCCGGCGTAACGCCGGAATCCTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCgt         <  1:8392114/93‑1 (MQ=255)

                                                                                aCTCTTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCgt         <  1:119515/93‑1 (MQ=255)

                                                                                  tctTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCgtgt       <  1:5377896/93‑1 (MQ=255)

                                                                                   ctTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGtc      >  2:209955/1‑93 (MQ=255)

                                                                                   ctTGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGtc      <  2:1684120/93‑1 (MQ=255)

                                                                                    ttGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGtca     <  1:5065647/93‑1 (MQ=255)

                                                                                    ttGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGtca     <  2:4024970/93‑1 (MQ=255)

                                                                                    ttGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGtca     >  2:3305145/1‑93 (MQ=255)

                                                                                    ttGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGtca     <  2:2911488/93‑1 (MQ=255)

                                                                                     tGTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGtcat    >  2:6014850/1‑92 (MQ=255)

                                                                                      gTCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGtcaat   >  2:806665/1‑93 (MQ=255)

                                                                                       tCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGtcaatc  <  2:646812/93‑1 (MQ=255)

                                                                                       tCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGtcaatc  >  2:3401211/1‑93 (MQ=255)

                                                                                       tCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGtcaatc  >  1:2437940/1‑93 (MQ=255)

                                                                                       tCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGtcaatc  >  1:8262870/1‑93 (MQ=255)

                                                                                       tCCGGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGtcaatc  <  1:7423605/93‑1 (MQ=255)

AACAGATACCAGACATCCGCCGCTGCGTATTCACACTGACGTTCGGTCAGCGGTCTGGCCAGCCAGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATC  >  NC_000913/1885537‑1885716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`