BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,116,149	A→G	V93A (GTC→GCC)	wcaK ←	putative galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,116,149	0	A	G	99.3%	959.3 / ‑4.3	285	V93A (GTC→GCC)	wcaK	putative galactokinase
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (113/170); total (114/171)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 306 total aligned reads displayed.

TGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTC  >  NC_000913/2116059‑2116238

tGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGcg                                                                                         >  1:194786/1‑93 (MQ=255)

tGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGcg                                                                                         >  2:1375572/1‑93 (MQ=255)

tGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGCAGGTGGcg                                                                                         >  2:1886338/1‑93 (MQ=255)

 ggATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGgcgc                                                                                        >  1:1089872/1‑93 (MQ=255)

  gATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTCGGCGATGGCGATATTGCGCAGCTTGCCCGTGTCGGTGGcgcg                                                                                       >  2:2996034/1‑93 (MQ=255)

   aTAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCTGTGTCGGTGGCGCGt                                                                                      <  1:8588595/93‑1 (MQ=255)

     aaTGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGCTATTGAGCAGCTTGCCAGTGCGGGTGGCGCGTga                                                                                    >  1:1329508/1‑93 (MQ=255)

     aaTGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTga                                                                                    <  1:7969437/93‑1 (MQ=255)

      aTGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTgag                                                                                   >  2:3864731/1‑93 (MQ=255)

       tGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTgaga                                                                                  <  1:6789965/93‑1 (MQ=255)

       tGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTgaga                                                                                  <  1:2236790/93‑1 (MQ=255)

       tGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTgaga                                                                                  <  1:4882542/93‑1 (MQ=255)

        ggCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGagag                                                                                 <  1:417423/93‑1 (MQ=255)

         gCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGc                                                                                >  1:8768790/1‑93 (MQ=255)

          cgtcgtAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCa                                                                               >  1:7728832/1‑93 (MQ=255)

            tcgtACCCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAAt                                                                             <  2:5024428/93‑1 (MQ=255)

            tcgtAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAAt                                                                             >  2:3616814/1‑93 (MQ=255)

            tcgtAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAAt                                                                             >  2:478258/1‑93 (MQ=255)

               tAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATAcc                                                                          >  2:1665672/1‑93 (MQ=255)

                aaCCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCt                                                                         <  1:5737631/93‑1 (MQ=255)

                aaCCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCt                                                                         >  2:1617601/1‑93 (MQ=255)

                 aCCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTg                                                                        >  2:1096095/1‑93 (MQ=255)

                  ccTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGa                                                                       <  1:7950726/93‑1 (MQ=255)

                   cTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGTGTGAGAGCAATACCTGAt                                                                      <  1:2949213/93‑1 (MQ=255)

                      aCAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGGGTCGGTGGAGCGTGAGAGCAATACCTGATGCt                                                                   >  1:3064091/1‑93 (MQ=255)

                       cagcagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTg                                                                  >  1:1605492/1‑93 (MQ=255)

                        agcagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTgg                                                                 >  1:246962/1‑93 (MQ=255)

                         gcagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGt                                                                <  1:281212/93‑1 (MQ=255)

                         gcagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGt                                                                >  2:8121642/1‑93 (MQ=255)

                          cagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTa                                                               >  1:4431451/1‑93 (MQ=255)

                           agGCGCACGAAGTCGGTGAATCACTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGGGTGAGAGCAATACCTCGTGTTGGTAg                                                              >  2:2335840/1‑93 (MQ=255)

                            ggCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAgg                                                             >  1:2697847/1‑92 (MQ=255)

                              cgcACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAgcgg                                                           <  2:2677023/93‑1 (MQ=255)

                               gcACGAAGTCGGTGCATCGCGGGGTTATGGTGATCTTGCGCAGCTCGCCAGTGCCGGTGCCGCGAGAGAGCAATACCTGATCCTGGTAgggac                                                          <  2:7775278/93‑5 (MQ=255)

                               gcACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAgcggc                                                          >  1:2042932/1‑93 (MQ=255)

                               gcACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAgcggc                                                          <  1:7450629/93‑1 (MQ=255)

                               gcACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAgcggc                                                          >  1:3280891/1‑93 (MQ=255)

                                cACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAgcggcg                                                         >  1:8135310/1‑93 (MQ=255)

                                cACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAgcggcg                                                         >  2:4491342/1‑93 (MQ=255)

                                 aCGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGa                                                        >  1:5519266/1‑93 (MQ=255)

                                  cGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGag                                                       >  2:7233272/1‑92 (MQ=255)

                                  cGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGAc                                                       <  1:545637/93‑1 (MQ=255)

                                    aaGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACgg                                                     >  1:4827216/1‑93 (MQ=255)

                                    aaGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTCAGAGCAATACCTGATGCTGGTAGCGGCGACgg                                                     >  2:5515859/1‑93 (MQ=255)

                                    aaGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGCGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACgg                                                     >  1:3575247/1‑93 (MQ=255)

                                     aGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACgga                                                    <  1:1781899/93‑1 (MQ=255)

                                     aGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACgga                                                    <  1:4626252/93‑1 (MQ=255)

                                     aGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACgga                                                    >  1:5147065/1‑93 (MQ=255)

                                      gTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACggag                                                   >  1:6141391/1‑93 (MQ=255)

                                       tCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACggagg                                                  <  2:5702522/93‑1 (MQ=255)

                                         ggTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGAc                                                <  2:308638/93‑1 (MQ=255)

                                         ggTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGAc                                                <  2:7626776/93‑1 (MQ=255)

                                         ggTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGAc                                                <  1:6899481/93‑1 (MQ=255)

                                           tGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACtt                                              >  1:1469453/1‑93 (MQ=255)

                                             aaTCCCTGGGCGATGGCGCTATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGGAGCGGCGAGGGAGGGTtttt                                            >  1:5918690/1‑93 (MQ=255)

                                             aaTCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACtttt                                            <  1:5833262/93‑1 (MQ=255)

                                                 ccTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTaa                                        >  1:4908838/1‑93 (MQ=255)

                                                 ccTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTaa                                        <  2:2163512/93‑1 (MQ=255)

                                                  cTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAAc                                       <  2:6389227/93‑1 (MQ=255)

                                                  cTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAAc                                       >  2:519578/1‑93 (MQ=255)

                                                  cTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAAc                                       <  2:6483271/93‑1 (MQ=255)

                                                   tGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAAcg                                      >  2:6315683/1‑93 (MQ=255)

                                                   tGGCCGATGTCGACATAGTGCAGTCTGCAAGGGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGTAGGACTTTTTTAAcg                                      <  2:3534406/93‑1 (MQ=255)

                                                    gggCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAAcgc                                     >  2:598531/1‑93 (MQ=255)

                                                     ggcgatggcgatATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGCCTTTTTTAAcgcg                                    <  2:4149531/93‑1 (MQ=255)

                                                      gcgatggggatATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACgcgc                                   <  1:1255730/93‑1 (MQ=255)

                                                       cgatggcgatATTGCGCAGCTTGCCAGTGTCGGTTGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGcc                                  <  1:8333483/93‑1 (MQ=255)

                                                       cgatggcgatATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGcc                                  <  1:658816/93‑1 (MQ=255)

                                                        gatggcgatATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGccc                                 <  1:8217301/93‑1 (MQ=255)

                                                        gatggcgatATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGccc                                 >  2:4410211/1‑93 (MQ=255)

                                                         atggcgatATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGTTGCTGGTAGCGGCGACGGAGGACTTTTTTAAcgcgcgcg                                >  1:4738022/1‑93 (MQ=255)

                                                           ggcgatATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGAc                              <  2:1793467/93‑1 (MQ=255)

                                                           ggcgatATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGAc                              <  2:6332029/93‑1 (MQ=255)

                                                             cgatATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACaa                            <  2:6675706/93‑1 (MQ=255)

                                                             cgatATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACaa                            <  2:8207570/93‑1 (MQ=255)

                                                             cgatATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACaa                            <  1:7255379/93‑1 (MQ=255)

                                                              gatATTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAAc                           >  1:3782722/1‑93 (MQ=255)

                                                                 atTGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAAcgcc                        >  2:2084445/1‑93 (MQ=255)

                                                                  ttGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAAcgccc                       <  1:3979078/93‑1 (MQ=255)

                                                                   tGCGCAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAAcgcccg                      <  2:7419869/93‑1 (MQ=255)

                                                                    gcgcAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAAcgcccgc                     <  1:544558/93‑1 (MQ=255)

                                                                      gcAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCccgccg                   >  1:2429425/1‑93 (MQ=255)

                                                                       cAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCcgccgc                  <  1:3252264/93‑1 (MQ=255)

                                                                       cAGCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCcgccgc                  <  1:7592695/93‑1 (MQ=255)

                                                                         gCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCgcgc                <  2:8441871/93‑1 (MQ=255)

                                                                         gCTTGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCgcgc                <  1:8048664/93‑1 (MQ=255)

                                                                            tGCGAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgt             <  1:3523767/93‑1 (MQ=255)

                                                                            tGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgt             <  1:7311358/93‑1 (MQ=255)

                                                                            tGCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgt             <  1:2385984/93‑1 (MQ=255)

                                                                             gCCAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgtt            >  1:2340422/1‑93 (MQ=255)

                                                                               cAGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttgt          <  2:4947347/93‑1 (MQ=255)

                                                                                aGTGTCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttgtg         >  2:3387943/1‑93 (MQ=255)

                                                                                 gtgtCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttgtgt        >  2:2382142/1‑93 (MQ=255)

                                                                                   gtCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttgtgttg      <  1:6055944/93‑1 (MQ=255)

                                                                                    tCGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGATTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTgttgtgttgt     <  2:6917850/93‑1 (MQ=255)

                                                                                     cGGTGTCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTgttgtt    <  2:3948583/93‑1 (MQ=255)

                                                                                     cGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTgttgtt    >  2:5621612/1‑93 (MQ=255)

                                                                                     cGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTgttgtt    >  1:951366/1‑93 (MQ=255)

                                                                                     cGGTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTgttgtt    <  2:857852/93‑1 (MQ=255)

                                                                                       gTGGCGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTc  >  1:5117290/1‑93 (MQ=255)

TGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTC  >  NC_000913/2116059‑2116238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`