BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,210,202	A→G	D5D (GAT→GAC)	yehS ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,210,202	0	A	G	99.7%	1172.8 / NA	334	D5D (GAT→GAC)	yehS	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (148/185); minor base T (0/1); total (148/186)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 340 total aligned reads displayed.

TTCCGCGGTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTAAGCTTT  >  NC_000913/2210112‑2210291

    gcgGTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTa                                                                                     >  2:6753492/1‑93 (MQ=255)

      ggTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACt                                                                                   <  1:4799404/93‑1 (MQ=255)

      ggTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACt                                                                                   <  2:3574902/93‑1 (MQ=255)

      ggTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACt                                                                                   >  1:2186427/1‑93 (MQ=255)

       gTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACtt                                                                                  >  2:2250449/1‑93 (MQ=255)

        tGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTa                                                                                 <  1:8110710/93‑1 (MQ=255)

         ggCTTCGACATTACCCAGAGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAg                                                                                >  2:5316285/1‑93 (MQ=255)

          gCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGc                                                                               >  1:2878049/1‑93 (MQ=255)

           gttCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCa                                                                              <  1:4085340/92‑1 (MQ=255)

           cTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCa                                                                              <  2:855922/93‑1 (MQ=255)

             tCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATg                                                                            >  1:353541/1‑93 (MQ=255)

             tCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATg                                                                            <  2:316958/93‑1 (MQ=255)

              cGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGa                                                                           <  2:3350958/93‑1 (MQ=255)

               gACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAg                                                                          >  2:1881891/1‑93 (MQ=255)

                aCATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAgg                                                                         >  2:3019018/1‑93 (MQ=255)

                  aTTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGcc                                                                       >  1:1612731/1‑93 (MQ=255)

                  aTTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGcc                                                                       >  1:1871019/1‑93 (MQ=255)

                   ttACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCt                                                                      <  2:3645496/93‑1 (MQ=255)

                   ttACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCt                                                                      <  2:131171/93‑1 (MQ=255)

                    tACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCtt                                                                     >  1:1011289/1‑93 (MQ=255)

                       ccAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGATGCCTTCAg                                                                  >  1:1727935/1‑93 (MQ=255)

                       ccAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAg                                                                  <  1:2423379/93‑1 (MQ=255)

                         aGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGgt                                                                <  1:1803896/93‑1 (MQ=255)

                         aGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGgt                                                                >  2:7688163/1‑93 (MQ=255)

                            gcCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGtt                                                             >  1:5287040/1‑93 (MQ=255)

                             ccAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTtg                                                            >  1:786655/1‑93 (MQ=255)

                             ccAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTtg                                                            >  2:8049742/1‑93 (MQ=255)

                              cAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTtga                                                           >  1:906574/1‑93 (MQ=255)

                               aGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTtgat                                                          >  1:8374022/1‑93 (MQ=255)

                               aGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTtgat                                                          >  2:8346358/1‑93 (MQ=255)

                               aGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTtgat                                                          <  1:4982838/93‑1 (MQ=255)

                               aGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTtgat                                                          <  1:2827569/93‑1 (MQ=255)

                                gAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTtgatg                                                         >  1:7741079/1‑93 (MQ=255)

                                gAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTtgatg                                                         <  2:6018748/93‑1 (MQ=255)

                                gAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTtgatg                                                         <  1:336584/93‑1 (MQ=255)

                                gAATACGCACCAGGTCATTATTATTGGCTTGCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTtgatg                                                         <  2:2580111/93‑1 (MQ=255)

                                 aaTACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTtgatga                                                        <  2:4342895/93‑1 (MQ=255)

                                 aaTACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTtgatga                                                        >  2:5624667/1‑93 (MQ=255)

                                  aTACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAg                                                       >  2:2449559/1‑93 (MQ=255)

                                  aTACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAg                                                       <  2:4095349/93‑1 (MQ=255)

                                      gCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCaa                                                   >  1:6763240/1‑93 (MQ=255)

                                       cACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCaaa                                                  >  1:6256278/1‑93 (MQ=255)

                                        aCCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCaaaa                                                 <  2:8658434/93‑1 (MQ=255)

                                         ccAGGTCATTATTATTGGTTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCaaaaa                                                <  1:2662152/93‑1 (MQ=255)

                                            ggTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGcc                                             >  1:7175468/1‑93 (MQ=255)

                                              tCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAACAGCCAt                                           >  2:7260363/1‑93 (MQ=255)

                                              tCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCAt                                           >  2:7459024/1‑93 (MQ=255)

                                                attattattGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCAttt                                         >  2:3084794/1‑93 (MQ=255)

                                                attattattGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCAttt                                         >  1:3780765/1‑93 (MQ=255)

                                                  tattattGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTa                                       >  2:4730931/1‑93 (MQ=255)

                                                  tattattGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTa                                       <  2:5749919/93‑1 (MQ=255)

                                                    ttattGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGc                                     >  1:4769367/1‑93 (MQ=255)

                                                     tattGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCa                                    <  1:314588/93‑1 (MQ=255)

                                                     tattGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCa                                    >  1:5851781/1‑93 (MQ=255)

                                                     tattGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCa                                    <  1:3745331/93‑1 (MQ=255)

                                                     tattGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCa                                    >  1:3654786/1‑93 (MQ=255)

                                                      attGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAg                                   <  1:566023/93‑1 (MQ=255)

                                                      attGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAg                                   <  1:4208035/93‑1 (MQ=255)

                                                       ttGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGt                                  <  2:4986571/93‑1 (MQ=255)

                                                       ttGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGt                                  >  2:10303/1‑93 (MQ=255)

                                                        tGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTc                                 <  1:4652081/93‑1 (MQ=255)

                                                         ggCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCt                                <  2:1085511/93‑1 (MQ=255)

                                                         ggCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCt                                <  2:7566548/93‑1 (MQ=255)

                                                         ggCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCt                                >  1:6940093/1‑93 (MQ=255)

                                                          gCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCtt                               <  2:1356493/93‑1 (MQ=255)

                                                          gCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCtt                               >  1:6858753/1‑93 (MQ=255)

                                                           cTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCttt                              <  2:1643090/93‑1 (MQ=255)

                                                            tttCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCtttt                             >  1:5353018/1‑93 (MQ=255)

                                                             ttCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTa                            <  2:3824287/93‑1 (MQ=255)

                                                              tCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTAc                           <  2:7989341/93‑1 (MQ=255)

                                                                aaaaTGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAg                         <  1:6096272/93‑1 (MQ=255)

                                                                 aaaTGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAgg                        >  2:4558160/1‑93 (MQ=255)

                                                                  aaTGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGc                       <  1:4588047/93‑1 (MQ=255)

                                                                  aaTGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGc                       <  1:3021694/93‑1 (MQ=255)

                                                                    tGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCa                     >  1:7592760/1‑93 (MQ=255)

                                                                     gTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCaa                    >  1:45552/1‑93 (MQ=255)

                                                                     gTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCaa                    >  2:2666689/1‑93 (MQ=255)

                                                                     gTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCaa                    <  2:4775310/93‑1 (MQ=255)

                                                                      tAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAAt                   <  2:6846722/93‑1 (MQ=255)

                                                                      tAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAAt                   >  2:6630357/1‑93 (MQ=255)

                                                                       aGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATc                  <  2:2101995/93‑1 (MQ=255)

                                                                        gcgcACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTAAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCg                 >  1:8660041/1‑93 (MQ=255)

                                                                          gcACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGcc               <  2:2313320/93‑1 (MQ=255)

                                                                           cACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCct              >  1:131070/1‑93 (MQ=255)

                                                                            aCGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCctc             >  1:6438863/1‑93 (MQ=255)

                                                                            aCGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCctc             >  1:6659778/1‑93 (MQ=255)

                                                                             cGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCctct            >  1:8769803/1‑93 (MQ=255)

                                                                              gCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCtt           <  1:7294114/93‑1 (MQ=255)

                                                                              gCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCtt           >  1:5441056/1‑93 (MQ=255)

                                                                               cTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCttt          <  1:829168/93‑1 (MQ=255)

                                                                                tGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGGGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCtttt         >  2:3250115/1‑93 (MQ=255)

                                                                                 gcgcAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTa        <  2:6457570/93‑1 (MQ=255)

                                                                                  cgcAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTaa       <  2:630045/93‑1 (MQ=255)

                                                                                    cAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTAAGc     <  2:3901446/93‑1 (MQ=255)

                                                                                     aGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTAAGCt    >  2:6923036/1‑93 (MQ=255)

                                                                                     aGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTAAGCt    >  2:715533/1‑93 (MQ=255)

                                                                                     aGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTAAGCt    >  2:5136311/1‑93 (MQ=255)

                                                                                      gAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTAAGCtt   >  1:2783843/1‑93 (MQ=255)

                                                                                      gAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTAAGCtt   >  1:2597347/1‑93 (MQ=255)

                                                                                       aaTGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTAAGCttt  >  2:5983762/1‑93 (MQ=255)

TTCCGCGGTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTAAGCTTT  >  NC_000913/2210112‑2210291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`