BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,252,917	C→T	R97H (CGC→CAC)	yeiK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,252,917	0	C	T	99.7%	1045.5 / NA	298	R97H (CGC→CAC)	yeiK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (168/129); minor base A (1/0); total (169/129)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 312 total aligned reads displayed.

AGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGG  >  NC_000913/2252827‑2253005

aGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCtggtgg                                                                                        <  1:4765895/93‑1 (MQ=255)

aGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCtggtgg                                                                                        <  1:3932559/93‑1 (MQ=255)

 gCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTggg                                                                                       >  2:8572329/1‑93 (MQ=255)

   ggACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTc                                                                                     <  2:8722143/93‑1 (MQ=255)

      ccAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGc                                                                                  <  1:2017237/93‑1 (MQ=255)

       cAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGca                                                                                 <  2:8292133/93‑2 (MQ=255)

       cAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCg                                                                                 >  2:8002775/1‑93 (MQ=255)

       cAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCg                                                                                 <  1:2827735/93‑1 (MQ=255)

        aaCCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCgg                                                                                >  1:5568201/1‑93 (MQ=255)

          ccGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCt                                                                              >  1:8369385/1‑93 (MQ=255)

          ccGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCt                                                                              >  1:4583763/1‑93 (MQ=255)

          ccGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCt                                                                              >  1:4558705/1‑93 (MQ=255)

           cGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTc                                                                             >  1:7085247/1‑93 (MQ=255)

             gCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGa                                                                           >  2:3265705/1‑93 (MQ=255)

               cccAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAAt                                                                         <  1:542704/92‑1 (MQ=255)

                ccAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATa                                                                        >  1:5520390/1‑93 (MQ=255)

                 cAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATAc                                                                       <  2:6904540/93‑1 (MQ=255)

                  agagTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATAcc                                                                      >  2:6630335/1‑93 (MQ=255)

                     gTGATGTCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCggg                                                                   >  2:4048358/1‑93 (MQ=255)

                     gTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCggg                                                                   >  2:6222525/1‑93 (MQ=255)

                        atatCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCa                                                                <  1:5241745/93‑1 (MQ=255)

                          atCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGGCATc                                                              >  1:8784279/1‑93 (MQ=255)

                          atCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATc                                                              >  1:8197626/1‑93 (MQ=255)

                           tCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATcc                                                             <  1:3424869/93‑1 (MQ=255)

                           tCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATcc                                                             >  2:4618014/1‑93 (MQ=255)

                             gCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAg                                                           >  1:1357624/1‑93 (MQ=255)

                             gCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCAGGCCATCCAg                                                           >  2:3948221/1‑93 (MQ=255)

                              ccATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCCGt                                                          >  2:3402856/1‑93 (MQ=255)

                              ccATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGt                                                          >  2:2864031/1‑93 (MQ=255)

                               cATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTc                                                         <  1:6143978/93‑1 (MQ=255)

                               cATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTc                                                         <  2:4779278/93‑1 (MQ=255)

                               cATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTc                                                         <  1:2513809/93‑1 (MQ=255)

                                aTCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTcc                                                        <  2:2132816/93‑1 (MQ=255)

                                aTCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTcc                                                        >  2:5363759/1‑93 (MQ=255)

                                aTCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTcc                                                        <  2:6064625/93‑1 (MQ=255)

                                 tCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAGTACCGGGCCATCCAGTCCg                                                       <  2:6523751/93‑1 (MQ=255)

                                    cTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGtt                                                    >  2:2600325/1‑93 (MQ=255)

                                    cTTGCCATCAGGGTATCAATGATATATTGCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGtt                                                    <  2:4138790/93‑1 (MQ=255)

                                     ttGCCATCAGGGTATCAATGATATATTTCCCCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGttt                                                   <  2:1274385/93‑1 (MQ=255)

                                     ttGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGttt                                                   >  1:8606307/1‑93 (MQ=255)

                                     ttGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAAAACAGGGCCATCCAGTCCGGctt                                                   >  2:1998913/1‑93 (MQ=255)

                                       gCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTcc                                                 >  1:2113468/1‑92 (MQ=255)

                                        ccATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCCTCCAGTCCGGTTTGAc                                                >  2:8423696/1‑93 (MQ=255)

                                        ccATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCAc                                                >  2:188/1‑93 (MQ=255)

                                        ccATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCAc                                                <  1:4493266/93‑1 (MQ=255)

                                         cATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCAcc                                               >  1:233551/1‑93 (MQ=255)

                                          aTCAGGGTATCGATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATAACGGGCCATCCAGTCCGGTTTCACCg                                              >  2:1113929/1‑93 (MQ=255)

                                          aTCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCg                                              <  2:5751824/93‑1 (MQ=255)

                                          aTCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCg                                              >  1:1241935/1‑93 (MQ=255)

                                          aTCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCg                                              >  1:6331152/1‑93 (MQ=255)

                                          aTCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCg                                              >  2:1618884/1‑93 (MQ=255)

                                              gggTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGaa                                          <  1:1466410/93‑1 (MQ=255)

                                              gggTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGaa                                          >  2:5526918/1‑93 (MQ=255)

                                              gggTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGaa                                          >  1:24678/1‑93 (MQ=255)

                                               ggTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAat                                         >  2:2447672/1‑93 (MQ=255)

                                               ggTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAat                                         <  1:5870741/93‑1 (MQ=255)

                                                gTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAata                                        <  1:7141685/93‑1 (MQ=255)

                                                gTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGACAGCGGCTCTAATACCGGGCCATCCACACCCGTTTCACCGTGAatt                                        >  2:2952320/1‑92 (MQ=255)

                                                  aTCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAAtatt                                      <  2:7336134/93‑1 (MQ=255)

                                                  aTCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAAtatt                                      >  1:3805550/1‑93 (MQ=255)

                                                  aTCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAAtatt                                      <  1:2722524/93‑1 (MQ=255)

                                                   tCAATGATATATTTCACCGCATGCGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAAtatta                                     <  2:4813190/93‑1 (MQ=255)

                                                   tCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCCTCCAGTCCGGTTTCCCCGCGCAtagta                                     >  1:1623922/1‑93 (MQ=255)

                                                   tCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAAtatta                                     >  2:8754348/1‑93 (MQ=255)

                                                   tCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAAtatta                                     >  1:1426624/1‑93 (MQ=255)

                                                   acAATCATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGCTCAGCGGCTCAAATACCGGGCCATCCAGTCCGGTTTCACCGTGAAtatta                                     <  1:3812954/92‑1 (MQ=255)

                                                    taaTGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAAtattat                                    <  2:4533774/92‑1 (MQ=255)

                                                     aaTGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCCTCCAGTCCGGTTTCACCGTGAATATTATc                                   >  1:662671/1‑93 (MQ=255)

                                                       tGATATATTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCgg                                 >  1:640761/1‑93 (MQ=255)

                                                         atatatTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCa                               >  1:1000780/1‑93 (MQ=255)

                                                          tatatTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCaa                              <  2:641851/93‑1 (MQ=255)

                                                           atatTTCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAAc                             >  1:1773092/1‑93 (MQ=255)

                                                               ttCACCGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGAtt                         >  1:4981428/1‑93 (MQ=255)

                                                                  tccGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATttgt                      <  2:1742872/92‑1 (MQ=255)

                                                                   ccGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATttgtt                     <  2:4859435/93‑1 (MQ=255)

                                                                   ccGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATttgtt                     <  1:679853/93‑1 (MQ=255)

                                                                   ccGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATctgtt                     <  1:8725220/93‑1 (MQ=255)

                                                                    cGCATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGCTTCACCGTGAATATTATCGGCAACGATttgttg                    >  1:3994259/1‑93 (MQ=255)

                                                                      cATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGAc                  >  1:2255648/1‑93 (MQ=255)

                                                                      cATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGAc                  >  1:3031236/1‑93 (MQ=255)

                                                                      cATGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGAc                  >  1:6234540/1‑93 (MQ=255)

                                                                        tGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGc                >  2:7001472/1‑93 (MQ=255)

                                                                        tGAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGc                >  1:7446801/1‑93 (MQ=255)

                                                                         gAGTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCa               >  1:512121/1‑93 (MQ=255)

                                                                           gTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATa             <  1:3753774/93‑1 (MQ=255)

                                                                           gTGCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATa             <  1:6859407/93‑1 (MQ=255)

                                                                           gTGCTTTCTGCCTGGTGGGCCAGCGGCTCGAATACCGGGCCATCCAGTTCGGCTTCACCGCGTATATTATCCGCAACGATTTGTTGACGCATa             >  2:4888066/1‑93 (MQ=255)

                                                                             gCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAAt           >  1:1528227/1‑93 (MQ=255)

                                                                             gCTTTCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAAt           >  1:3152832/1‑93 (MQ=255)

                                                                               tttCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATgg         >  1:697663/1‑93 (MQ=255)

                                                                                ttCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATggg        <  1:4282286/93‑1 (MQ=255)

                                                                                tgcTGCCTGGTTGGTCATCGGCTCAAATCCCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATggg        <  1:1212240/91‑1 (MQ=255)

                                                                                 tCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGc       <  2:590694/93‑1 (MQ=255)

                                                                                 tCTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGAAGCATAATGGGc       >  1:720655/1‑93 (MQ=255)

                                                                                  cTGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCt      <  2:7428934/93‑1 (MQ=255)

                                                                                   tGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTTACGCATAATGGGCTg     >  1:4905069/1‑93 (MQ=255)

                                                                                   tGCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTg     >  1:7723300/1‑93 (MQ=255)

                                                                                    gCCTGGTGGGTCAGCGGCTCGAATACCGGGCCATCGAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGc    >  2:7641358/1‑93 (MQ=255)

                                                                                    gCCTGGAGGGTCAGCGGCTCGAATACCGGGCCATCCAGTTCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGc    >  2:7742879/1‑93 (MQ=255)

                                                                                     ccTGGTGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCg   <  2:3753806/93‑1 (MQ=255)

AGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGG  >  NC_000913/2252827‑2253005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`