BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,497,463	T→C	V258A (GTG→GCG)	b2380 →	putative sensor protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,497,463	0	T	C	99.7%	979.1 / ‑4.8	287	V258A (GTG→GCG)	b2380	putative sensor protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (131/155); total (131/156)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

Only 100 of 303 total aligned reads displayed.

ATTACGCAAGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAATCATCATTAAAAACA  >  NC_000913/2497371‑2497552

  tACGCAAGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCgg                                                                                         <  2:3472976/93‑1 (MQ=255)

    cGCAAGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCgggg                                                                                       >  1:7671724/1‑92 (MQ=255)

     gCAAGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTg                                                                                      >  1:2204580/1‑93 (MQ=255)

      cAAGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGa                                                                                     <  1:1048825/93‑1 (MQ=255)

       aaGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGaa                                                                                    <  1:8648357/93‑1 (MQ=255)

       aaGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGaa                                                                                    >  1:8448297/1‑93 (MQ=255)

       aaGGGCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGaa                                                                                    <  1:384301/93‑1 (MQ=255)

        aGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAAc                                                                                   >  2:575147/1‑93 (MQ=255)

        aGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAAc                                                                                   <  1:8401909/93‑1 (MQ=255)

        aGGGCTGCGAAATTATCCGCGATGACATTAAAGCCGATGCGGTGGCGATTACTAATACCGATGATGAGCTGGCCTATGTTGGCGCGGGGGAAc                                                                                   >  1:2968467/1‑93 (MQ=255)

         ggTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACa                                                                                  <  1:4728641/93‑1 (MQ=255)

            cTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATaa                                                                               <  2:6691711/93‑1 (MQ=255)

             tGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAAc                                                                              >  2:5456529/1‑93 (MQ=255)

             tGCAAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAAc                                                                              <  1:8480644/93‑1 (MQ=255)

               cGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTa                                                                            <  2:3385233/93‑1 (MQ=255)

               cGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTa                                                                            <  2:8053247/93‑1 (MQ=255)

                gAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTAt                                                                           >  2:2070145/1‑93 (MQ=255)

                gAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTAt                                                                           <  2:1050183/93‑1 (MQ=255)

                 aaaTTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATc                                                                          <  2:1334297/93‑1 (MQ=255)

                 aaaTTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATc                                                                          <  2:7491246/93‑1 (MQ=255)

                    ttATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGa                                                                       <  2:2867983/93‑1 (MQ=255)

                     tATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGaa                                                                      >  1:6881371/1‑93 (MQ=255)

                      aTCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAAt                                                                     >  2:8608928/1‑93 (MQ=255)

                      aTCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAAt                                                                     <  1:6534916/93‑1 (MQ=255)

                       tCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATg                                                                    <  1:8027539/93‑1 (MQ=255)

                        ctgcgATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATgg                                                                   >  2:7019909/3‑93 (MQ=255)

                        ccGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATgg                                                                   <  1:3468196/93‑1 (MQ=255)

                         cgcgATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGc                                                                  >  2:620328/1‑93 (MQ=255)

                         cgcgATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGc                                                                  <  2:4444310/93‑1 (MQ=255)

                           cgATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGa                                                                <  1:1684825/93‑1 (MQ=255)

                           cgATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGa                                                                <  2:3613356/93‑1 (MQ=255)

                              tGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGa                                                             <  2:4030259/93‑1 (MQ=255)

                               gACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGat                                                            >  2:6247316/1‑92 (MQ=255)

                                aCATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACt                                                           <  1:3629573/93‑1 (MQ=255)

                                aCATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACt                                                           >  2:5172296/1‑93 (MQ=255)

                                  aTTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTc                                                         >  2:1720154/1‑93 (MQ=255)

                                   ttCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCa                                                        >  1:6365554/1‑93 (MQ=255)

                                   ttCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCa                                                        >  1:8746767/1‑93 (MQ=255)

                                    tCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCAt                                                       >  2:5012754/1‑93 (MQ=255)

                                     cACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCAtt                                                      <  2:3022860/93‑1 (MQ=255)

                                     cACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCAtt                                                      <  2:731175/93‑1 (MQ=255)

                                     cACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCAtt                                                      <  2:7973086/93‑1 (MQ=255)

                                         ccGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTCTGCTGCCGCGGGTGAACATAACTATCACAATGGCGATGACTTCATTAGcc                                                  >  1:4642552/1‑93 (MQ=255)

                                         ccGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGcc                                                  <  1:4013357/93‑1 (MQ=255)

                                         ccGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGcc                                                  <  1:1465981/93‑1 (MQ=255)

                                           gATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCg                                                >  1:2552233/1‑93 (MQ=255)

                                             tGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGAc                                              <  1:7779359/93‑1 (MQ=255)

                                             tGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGAc                                              <  1:921797/93‑1 (MQ=255)

                                             tGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGAc                                              >  2:6763172/1‑93 (MQ=255)

                                               cGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTa                                            <  1:4549874/93‑1 (MQ=255)

                                                ggTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTAc                                           >  2:6838573/1‑93 (MQ=255)

                                                ggTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTAc                                           <  2:3843904/93‑1 (MQ=255)

                                                ggTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTAc                                           >  1:5954607/1‑93 (MQ=255)

                                                 gTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTAcc                                          >  1:2853456/1‑93 (MQ=255)

                                                  tGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTAccc                                         >  1:6214292/1‑93 (MQ=255)

                                                   ggCGATTACAAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCg                                        >  2:5817647/1‑93 (MQ=255)

                                                    gCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGt                                       <  2:1523609/93‑1 (MQ=255)

                                                    gCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGt                                       <  1:414806/93‑1 (MQ=255)

                                                    gCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGt                                       <  2:8345369/93‑1 (MQ=255)

                                                     cGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTc                                      <  2:7789764/93‑1 (MQ=255)

                                                       aTTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAg                                    <  1:440596/93‑1 (MQ=255)

                                                       aTTACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAg                                    >  2:8430172/1‑93 (MQ=255)

                                                        ttACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAgg                                   >  2:5948920/1‑93 (MQ=255)

                                                        ttACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAgg                                   <  2:1542419/93‑1 (MQ=255)

                                                        ttACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAgg                                   >  2:4335264/1‑93 (MQ=255)

                                                        ttACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAgg                                   <  2:6533035/93‑1 (MQ=255)

                                                         tACTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTCGCCAGACTACCCGTCAGGc                                  >  2:7078723/1‑93 (MQ=255)

                                                          aCTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCg                                 >  2:6864194/1‑93 (MQ=255)

                                                           cTAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGa                                <  2:4523131/93‑1 (MQ=255)

                                                            tAATACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGAt                               <  1:751857/93‑1 (MQ=255)

                                                              aTACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGa                             <  2:7090408/93‑1 (MQ=255)

                                                              aTACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGa                             >  2:5218849/1‑93 (MQ=255)

                                                               tACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGaa                            >  1:8173192/1‑93 (MQ=255)

                                                               tACCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGaa                            <  2:1801064/93‑1 (MQ=255)

                                                                aCCGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAAt                           <  1:7139442/93‑1 (MQ=255)

                                                                 ccGATCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAAtt                          <  2:2855175/93‑1 (MQ=255)

                                                                     tCATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACgg                      >  2:3196189/1‑93 (MQ=255)

                                                                      cATGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGa                     <  2:4871965/93‑1 (MQ=255)

                                                                       aTGTGCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGaa                    >  2:86808/1‑93 (MQ=255)

                                                                        tgtgCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGaaa                   >  2:5560052/1‑93 (MQ=255)

                                                                        tgtgCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGCaaa                   >  1:322298/1‑93 (MQ=255)

                                                                         gtgCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGaaaa                  >  1:140062/1‑93 (MQ=255)

                                                                           gCTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAat                >  2:1495455/1‑93 (MQ=255)

                                                                            cTGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAatc               <  2:421606/93‑1 (MQ=255)

                                                                             tGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTCCCCGTCAGGCGATGAATTACGGAAAAatca              <  2:5356426/93‑1 (MQ=255)

                                                                             tGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAatca              >  2:4212344/1‑93 (MQ=255)

                                                                             tGGCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAatca              >  1:7432195/1‑93 (MQ=255)

                                                                               gCCTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAatcatc            <  1:7894278/93‑1 (MQ=255)

                                                                                ccTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAAtcatca           >  2:2308128/1‑93 (MQ=255)

                                                                                ccTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAAtcatca           <  2:3966164/93‑1 (MQ=255)

                                                                                ccTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAAtcatca           <  2:7880394/93‑1 (MQ=255)

                                                                                ccTATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAAtcatca           <  2:6456222/93‑1 (MQ=255)

                                                                                  tATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAATCATCAtt         >  1:1946471/1‑93 (MQ=255)

                                                                                  tATGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAATCATCAtt         >  1:1280701/1‑93 (MQ=255)

                                                                                   aTGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAatca              <  1:5417916/87‑1 (MQ=255)

                                                                                   aTGTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAatca              >  2:5417916/1‑87 (MQ=255)

                                                                                     gTTGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAATCATCATTaaa      <  1:1151358/93‑1 (MQ=255)

                                                                                      ttGGCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAACTCATCATTaaaa     >  1:5280871/1‑93 (MQ=255)

                                                                                        ggCGCGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAATCATCATTAAAAAc   >  2:5654595/1‑93 (MQ=255)

                                                                                         gcgcGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAATCATCATTAAAAACa  <  1:5600019/93‑1 (MQ=255)

ATTACGCAAGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTTGGCGTGGGTGAACATAACTATCAGAATGGCGATGACTTCATTAGCCCGACTACCCGTCAGGCGATGAATTACGGAAAAATCATCATTAAAAACA  >  NC_000913/2497371‑2497552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`