BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,616,953	T→C	S410G (AGT→GGT)	uraA ←	UraA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,616,953	0	T	C	99.7%	984.2 / ‑5.2	286	S410G (AGT→GGT)	uraA	UraA
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (117/168); total (118/168)
Fisher's exact test for biased strand distribution p-value = 4.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

Only 100 of 293 total aligned reads displayed.

CGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCG  >  NC_000913/2616863‑2617044

cGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGa                                                                                           <  1:5205851/93‑1 (MQ=255)

cGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGa                                                                                           <  1:3738696/93‑1 (MQ=255)

 gggCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGAt                                                                                          >  1:4596295/1‑93 (MQ=255)

 gggCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGAt                                                                                          <  2:5994722/93‑1 (MQ=255)

  ggCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATc                                                                                         <  2:8476589/93‑1 (MQ=255)

    cagcaACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAg                                                                                       <  1:715997/93‑1 (MQ=255)

    cagcaACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAg                                                                                       <  2:5503099/93‑1 (MQ=255)

      gcaACACTGCCCTGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGtt                                                                                     >  1:8023756/1‑93 (MQ=255)

      gcaACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAgct                                                                                     >  2:1487758/1‑91 (MQ=255)

      gcaACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGtt                                                                                     >  1:5902134/1‑93 (MQ=255)

      gcaACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGtt                                                                                     >  1:1817265/1‑93 (MQ=255)

      gcaACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGtt                                                                                     <  2:6119996/93‑1 (MQ=255)

      gcaACACTGCCCGGTCGGTACATTATTCGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGtt                                                                                     <  1:2218229/93‑1 (MQ=255)

       caACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGttt                                                                                    <  2:2507793/93‑1 (MQ=255)

         acacTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGa                                                                                  <  2:7376059/93‑1 (MQ=255)

           acTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAg                                                                                <  1:7734143/93‑1 (MQ=255)

            cTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCTCTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGa                                                                               <  1:3591178/93‑1 (MQ=255)

            cTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGa                                                                               <  1:6981519/93‑1 (MQ=255)

              gCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATc                                                                             <  1:8156720/93‑1 (MQ=255)

               cccGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCa                                                                            >  2:1116964/1‑93 (MQ=255)

                ccGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCaa                                                                           <  2:5736312/93‑1 (MQ=255)

                 cggtcggtACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCaaa                                                                          <  1:2821523/93‑1 (MQ=255)

                  ggtcggtACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAAc                                                                         <  1:1798247/93‑1 (MQ=255)

                  ggtcggtACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAAc                                                                         >  2:7116337/1‑93 (MQ=255)

                    tcggtACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACtt                                                                       >  2:3035683/1‑93 (MQ=255)

                     cggtACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCACACTTa                                                                      >  2:5429748/1‑93 (MQ=255)

                      ggtACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTaa                                                                     >  2:1936608/1‑93 (MQ=255)

                      ggtACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTaa                                                                     <  1:3437870/93‑1 (MQ=255)

                      ggtACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTaa                                                                     <  1:3065289/93‑1 (MQ=255)

                       gtACATTATTTGTCTGTTATATCTGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAg                                                                    <  2:4752361/93‑1 (MQ=255)

                        tACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGc                                                                   <  1:4518198/93‑1 (MQ=255)

                        tACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGc                                                                   <  2:4519464/93‑1 (MQ=255)

                         aCATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGcc                                                                  >  2:2145762/1‑93 (MQ=255)

                           attattTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGa                                                                <  2:8771246/93‑1 (MQ=255)

                           attattTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGa                                                                <  1:2668919/93‑1 (MQ=255)

                            ttattTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGAt                                                               <  1:3281194/93‑1 (MQ=255)

                             tattTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATa                                                              <  2:5591501/93‑1 (MQ=255)

                              ttttGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGAtcc                                                             <  2:2863376/92‑3 (MQ=255)

                              attTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATAc                                                             >  1:6119917/1‑93 (MQ=255)

                                 tGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACcga                                                          <  2:798456/93‑1 (MQ=255)

                                  gTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACcgac                                                         <  2:6453860/93‑1 (MQ=255)

                                  gTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACcgac                                                         <  1:3243363/93‑1 (MQ=255)

                                   tCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGGAGATCAAACTTAAGCCGATACcgacg                                                        >  2:4424395/1‑93 (MQ=255)

                                   tCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACcgacg                                                        >  1:1707155/1‑93 (MQ=255)

                                   tCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACcgacg                                                        >  2:1083174/1‑93 (MQ=255)

                                    cTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACcgacga                                                       <  2:2311761/93‑1 (MQ=255)

                                    cTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACcgacga                                                       >  1:2339877/1‑93 (MQ=255)

                                    cTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTCCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACcgacga                                                       <  1:1224328/93‑1 (MQ=255)

                                     tGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGAt                                                      >  1:3153014/1‑93 (MQ=255)

                                      gTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATg                                                     <  2:1208477/93‑1 (MQ=255)

                                        tataTCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTATGCCGATACCGACGATGGt                                                   <  1:8578996/93‑1 (MQ=255)

                                        tataTCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGt                                                   <  2:1644448/93‑1 (MQ=255)

                                        tataTCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGt                                                   <  2:7704681/93‑1 (MQ=255)

                                        tataTCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCAACGATggc                                                   >  1:6731908/1‑92 (MQ=255)

                                         atatCCGCGTCTTCTGCGTCCAGCTCCACTTCTGCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTc                                                  <  1:4074663/93‑1 (MQ=255)

                                         atatCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTc                                                  >  2:6065757/1‑93 (MQ=255)

                                         atatCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTc                                                  <  1:6405030/93‑1 (MQ=255)

                                            tCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGcc                                               <  2:3152584/93‑1 (MQ=255)

                                            tCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGcc                                               <  1:3912004/93‑1 (MQ=255)

                                            tCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGCTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGcc                                               >  2:3476398/1‑93 (MQ=255)

                                             ccGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCATTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCa                                              >  2:6814109/1‑93 (MQ=255)

                                             ccGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCa                                              >  2:4982392/1‑93 (MQ=255)

                                              cgcgTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAg                                             >  1:5567001/1‑93 (MQ=255)

                                               gcgTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAgc                                            >  2:8330907/1‑93 (MQ=255)

                                               gcgTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAgc                                            <  1:6002277/93‑1 (MQ=255)

                                                 ggcttctGCCGCCAGCACCCCTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAgcgc                                          <  1:7242944/91‑1 (MQ=255)

                                                  tcttctGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGcc                                         >  2:3995065/1‑93 (MQ=255)

                                                  tcttctGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGcc                                         >  1:4277383/1‑93 (MQ=255)

                                                  tcttctGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGcc                                         >  1:6575716/1‑93 (MQ=255)

                                                        gCGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCt                                   <  2:2236722/93‑1 (MQ=255)

                                                         cGTCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCtt                                  <  1:1022803/93‑1 (MQ=255)

                                                           tCCAGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCtttt                                >  1:7423238/1‑93 (MQ=255)

                                                              aGCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAAc                             >  2:3772017/1‑93 (MQ=255)

                                                               gCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACt                            >  2:8091341/1‑93 (MQ=255)

                                                               gCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACt                            <  1:8705211/93‑1 (MQ=255)

                                                               gCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACt                            <  2:4887703/93‑1 (MQ=255)

                                                               gCACCACTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACt                            <  1:352043/93‑1 (MQ=255)

                                                                 cccacTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTcc                          <  2:8195479/92‑1 (MQ=255)

                                                                 accacTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTcc                          >  2:691947/1‑93 (MQ=255)

                                                                  ccacTTCTTCAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTccg                         >  1:4966178/1‑93 (MQ=255)

                                                                         ttcAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCcgccgcgcc                  <  2:2627152/93‑1 (MQ=255)

                                                                           cAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGa                <  1:4109619/93‑1 (MQ=255)

                                                                           cAGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGa                <  2:2391647/93‑1 (MQ=255)

                                                                           cAGGCCGCACCACACCGATCAGTTTAAAGATCAAACGTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGa                <  1:3843834/93‑1 (MQ=255)

                                                                            aGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGAt               >  2:1400646/1‑93 (MQ=255)

                                                                            aGGGCGCAGCACACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGAt               >  2:1805588/1‑93 (MQ=255)

                                                                                 gcagcaCACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTa          <  1:2741262/93‑1 (MQ=255)

                                                                                  cagcagACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCGAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTAc         <  1:4942571/93‑1 (MQ=255)

                                                                                   agcaCACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTAcc        <  1:1334796/93‑1 (MQ=255)

                                                                                   agcaCACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTAcc        >  2:3604123/1‑93 (MQ=255)

                                                                                   agcaCACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTAcc        >  2:8433001/1‑93 (MQ=255)

                                                                                    gcaCACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATTTTTAccc       <  2:5323101/93‑2 (MQ=255)

                                                                                    gcaCACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCt       >  2:6268105/1‑93 (MQ=255)

                                                                                    gcaCACCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCt       >  2:5135731/1‑93 (MQ=255)

                                                                                     cacaCCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCtt      <  1:2015333/93‑1 (MQ=255)

                                                                                     cacaCCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCtt      <  1:1605195/93‑1 (MQ=255)

                                                                                     cacaCCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCtt      <  1:5340653/93‑1 (MQ=255)

                                                                                     cacaCCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCtt      <  2:127921/93‑1 (MQ=255)

                                                                                       cacCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTcg    >  1:7629928/1‑93 (MQ=255)

                                                                                       cacCGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTcg    >  2:1866642/1‑93 (MQ=255)

CGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCG  >  NC_000913/2616863‑2617044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`