BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,701,551	T→C	Q178R (CAG→CGG)	rnc ←	RNase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,701,551	0	T	C	99.6%	914.1 / ‑4.7	264	Q178R (CAG→CGG)	rnc	RNase III
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (116/147); total (116/148)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01

Only 100 of 273 total aligned reads displayed.

CGGCTTGAACCTGTGCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCTGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTGGG  >  NC_000913/2701461‑2701640

cgGCTTGAACCTGTGCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCgg                                                                                         >  2:7940927/1‑93 (MQ=255)

  gCTTGAACCTGTGCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGAc                                                                                       <  2:1279011/93‑1 (MQ=255)

      gAACCTGTGCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTAcc                                                                                   >  2:4562803/1‑93 (MQ=255)

        aCCTGTGCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAg                                                                                 >  1:3189583/1‑93 (MQ=255)

         ccTGTGCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGa                                                                                >  1:2838875/1‑93 (MQ=255)

                cAACCACCGGTTCACTCAGTCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTcg                                                                         <  2:6266417/93‑1 (MQ=255)

                cAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTcg                                                                         >  2:313783/1‑93 (MQ=255)

                  accaccGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTcggc                                                                       <  2:347479/93‑1 (MQ=255)

                  accaccGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTcggc                                                                       >  1:2673761/1‑93 (MQ=255)

                   ccaccGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTcggca                                                                      <  2:6638749/93‑1 (MQ=255)

                   ccaccGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTcggca                                                                      <  2:2902566/93‑1 (MQ=255)

                    caccGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTcggcag                                                                     <  2:3903474/93‑1 (MQ=255)

                    caccGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTcggcag                                                                     <  2:4699472/93‑1 (MQ=255)

                    caccGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTcggcag                                                                     <  1:4384417/93‑1 (MQ=255)

                      ccGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTcggcagcg                                                                   >  2:6446130/1‑93 (MQ=255)

                        ggTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTcggcagcggc                                                                 <  1:3643040/93‑1 (MQ=255)

                        ggTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTcggcagcggc                                                                 >  2:1035136/1‑93 (MQ=255)

                        ggTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTcggcagcggc                                                                 >  2:5258306/1‑93 (MQ=255)

                         gTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTcggcagcggca                                                                >  1:4915558/1‑93 (MQ=255)

                         gTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTcggcagcggca                                                                <  2:373302/93‑1 (MQ=255)

                            cACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGAt                                                             <  2:3861458/93‑1 (MQ=255)

                             ccTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATg                                                            <  2:1982027/92‑1 (MQ=255)

                             aCTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATg                                                            <  1:6562403/93‑1 (MQ=255)

                             aCTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATg                                                            <  2:7496058/93‑1 (MQ=255)

                               tCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGc                                                          <  1:6496701/93‑1 (MQ=255)

                               tCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCACTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATggg                                                          >  2:7627160/1‑92 (MQ=255)

                                cAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCg                                                         <  2:2247699/93‑1 (MQ=255)

                                 aGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGa                                                        >  1:149867/1‑93 (MQ=255)

                                   gCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGAcc                                                      >  1:2428162/1‑93 (MQ=255)

                                    ccGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGAccc                                                     <  2:6057446/93‑1 (MQ=255)

                                     cGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCt                                                    <  1:1878260/93‑1 (MQ=255)

                                     cGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCt                                                    <  2:5914011/93‑1 (MQ=255)

                                     cGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCt                                                    <  1:3161139/93‑1 (MQ=255)

                                      gCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTg                                                   >  2:703573/1‑93 (MQ=255)

                                      gCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTg                                                   >  1:1995059/1‑93 (MQ=255)

                                      gCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTg                                                   <  1:5972689/93‑1 (MQ=255)

                                        tGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCa                                                 <  1:6262481/93‑1 (MQ=255)

                                        tGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCa                                                 <  1:5368448/93‑1 (MQ=255)

                                         gACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCaa                                                >  1:6973483/1‑93 (MQ=255)

                                          aCCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCaaa                                               >  2:2732119/1‑93 (MQ=255)

                                          aCCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCaaa                                               >  1:2584827/1‑93 (MQ=255)

                                          aCCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCaaa                                               >  1:7854907/1‑93 (MQ=255)

                                          aCCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCaaa                                               >  1:3997359/1‑93 (MQ=255)

                                           ccTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAat                                              >  2:3004486/1‑93 (MQ=255)

                                            cTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAata                                             <  1:77468/93‑1 (MQ=255)

                                               gtaGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTc                                          <  1:8281026/91‑1 (MQ=255)

                                                gaGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCt                                         <  1:8695925/92‑1 (MQ=255)

                                                  gTGGATAGTAAATTCCTGATCGTGCGCTTCGCCGCGTACCCGGACTACCAGATAAGTAGGCAGCTGCAGATGGCGACCCTGCAAATATTCTTg                                       <  1:6097605/93‑1 (MQ=255)

                                                   tGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTga                                      >  1:6875103/1‑92 (MQ=255)

                                                   tGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGc                                      <  2:1600364/93‑1 (MQ=255)

                                                    ggATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCTACCCTGCAAATCTTCTTGCa                                     >  1:4149263/1‑93 (MQ=255)

                                                      aTAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAg                                   <  1:7316057/93‑1 (MQ=255)

                                                      aTAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAg                                   >  2:6488224/1‑93 (MQ=255)

                                                       tAGTAAATTCCTGATCGTGCGCTTCTCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGTAGATGGCGACCCTGCAAATATTCTTGCAAgc                                  <  1:3733638/93‑1 (MQ=255)

                                                       tAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAgc                                  >  2:5028891/1‑93 (MQ=255)

                                                       tAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAgc                                  <  2:4157302/93‑1 (MQ=255)

                                                         gTAAATTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCGGATGGCGACCCTGCAAATATTCTTGCAAgcgc                                <  1:7868067/93‑1 (MQ=255)

                                                           aaaTTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGt                              <  2:2800083/93‑1 (MQ=255)

                                                           aaaTTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGt                              <  1:1640483/93‑1 (MQ=255)

                                                            aaTTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGtt                             <  2:8669861/93‑1 (MQ=255)

                                                             aTTCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGttt                            <  1:7129748/93‑1 (MQ=255)

                                                              ttCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGtttt                           <  2:8773763/93‑1 (MQ=255)

                                                              ttCCTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGtttt                           >  2:3425421/1‑93 (MQ=255)

                                                              ttCCTGATCGGGCGCTCCGCCACGTACCCGGACTACCAGATAAGTCGGCATCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGtttt                           >  2:7842673/1‑93 (MQ=255)

                                                                ccTGATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCg                         >  1:6325085/1‑93 (MQ=255)

                                                                   gATCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGAt                      >  1:8380490/1‑93 (MQ=255)

                                                                    aTCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGGCCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATc                     <  1:8747998/93‑1 (MQ=255)

                                                                    aTCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATc                     <  1:4252038/93‑1 (MQ=255)

                                                                    aTCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATc                     <  2:861122/93‑1 (MQ=255)

                                                                    aTCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATc                     >  2:5820050/1‑93 (MQ=255)

                                                                     tCGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCt                    >  1:1776519/1‑93 (MQ=255)

                                                                      cGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCtt                   <  1:1005552/93‑1 (MQ=255)

                                                                      cGTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCtt                   >  2:887053/1‑93 (MQ=255)

                                                                       gTGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCttt                  <  2:6001465/93‑1 (MQ=255)

                                                                        tGCGCTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCtttt                 <  2:7178561/93‑1 (MQ=255)

                                                                         gcgcTTCGCCACGTACCCGGACTACCATATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCttttt                <  1:1140786/93‑1 (MQ=255)

                                                                          cgcTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTg               <  1:8479980/93‑1 (MQ=255)

                                                                           gcTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGt              <  1:1079944/93‑1 (MQ=255)

                                                                           gcTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGt              >  1:1913043/1‑93 (MQ=255)

                                                                           gcTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGt              >  1:6754645/1‑93 (MQ=255)

                                                                            cTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGtt             <  2:2607333/93‑1 (MQ=255)

                                                                            cTTCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGtt             >  1:5136714/1‑93 (MQ=255)

                                                                             ttCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGttt            >  1:6416302/1‑93 (MQ=255)

                                                                              tCGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTa           <  1:1413846/93‑1 (MQ=255)

                                                                               cGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTAt          >  2:1399690/1‑93 (MQ=255)

                                                                               cGCCACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTAt          >  2:8497669/1‑93 (MQ=255)

                                                                                cgcACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATc         <  1:7449321/91‑1 (MQ=255)

                                                                                 ccACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCg        >  1:8080753/1‑93 (MQ=255)

                                                                                  cACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGc       >  2:6715851/1‑93 (MQ=255)

                                                                                  cACGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGc       >  2:5108949/1‑93 (MQ=255)

                                                                                   aCGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGcc      <  2:2568846/93‑1 (MQ=255)

                                                                                   aCGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGcc      >  1:521305/1‑93 (MQ=255)

                                                                                    cGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCt     <  2:2129958/93‑1 (MQ=255)

                                                                                    cGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCt     <  2:8312447/93‑1 (MQ=255)

                                                                                    cGTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCt     >  1:5500582/1‑93 (MQ=255)

                                                                                     gTACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGTAAGCGCGTTTTCGGATCTTTTTGTTTATGGCCTg    >  1:7621411/1‑93 (MQ=255)

                                                                                      tACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTgg   >  1:3348370/1‑93 (MQ=255)

                                                                                      tACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTgg   <  1:4575156/93‑1 (MQ=255)

                                                                                      tACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTgg   >  2:4401568/1‑93 (MQ=255)

                                                                                      tACCCGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTgg   <  2:3336084/93‑1 (MQ=255)

CGGCTTGAACCTGTGCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCTGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTGGG  >  NC_000913/2701461‑2701640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`