BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,719,936	A→G	Q655R (CAG→CGG)	yfiQ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,719,936	0	A	G	99.4%	1098.4 / ‑5.0	325	Q655R (CAG→CGG)	yfiQ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (178/145); total (180/145)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 335 total aligned reads displayed.

TGGCCCGCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCAGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTCTGGCAGTGAAT  >  NC_000913/2719846‑2720025

 ggCCCGCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCt                                                                                        <  2:2578302/93‑1 (MQ=255)

 ggCCCGCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCt                                                                                        <  1:5706474/93‑1 (MQ=255)

 ggCCCGCTATCTGGTGATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGTGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCt                                                                                        <  2:4374075/93‑1 (MQ=255)

    ccGCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCt                                                                                     >  1:5497722/1‑93 (MQ=255)

     cGCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTg                                                                                    >  1:7740092/1‑93 (MQ=255)

     cGCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTg                                                                                    <  2:6564463/93‑1 (MQ=255)

     cGCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTg                                                                                    >  2:1917613/1‑93 (MQ=255)

      gCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTgg                                                                                   >  2:3365842/1‑93 (MQ=255)

      gCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTgg                                                                                   <  1:2723297/93‑1 (MQ=255)

       cTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGt                                                                                  <  1:2487183/93‑1 (MQ=255)

        tATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTgggg                                                                                 >  2:7878318/1‑91 (MQ=255)

           cTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAg                                                                              >  2:3836844/1‑93 (MQ=255)

            tGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAgg                                                                             <  2:2800533/93‑1 (MQ=255)

              gTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCCGGtt                                                                           >  1:2055772/1‑93 (MQ=255)

              gTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGtt                                                                           >  2:5996769/1‑93 (MQ=255)

               ttATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGttt                                                                          <  1:3470636/93‑1 (MQ=255)

                 aTTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTcc                                                                        >  1:7375303/1‑93 (MQ=255)

                  ttCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCa                                                                       <  2:4512770/93‑1 (MQ=255)

                   tCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCaa                                                                      <  2:6895088/93‑1 (MQ=255)

                    cAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTTCAAc                                                                     >  2:4868187/1‑93 (MQ=255)

                    cAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAAc                                                                     <  2:4606009/93‑1 (MQ=255)

                    cAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAAc                                                                     <  2:5378996/93‑1 (MQ=255)

                     aGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGCTTCCAACt                                                                    >  1:987733/1‑93 (MQ=255)

                      ggggATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACtt                                                                   >  1:4718178/1‑93 (MQ=255)

                      ggggATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACtt                                                                   <  1:225978/93‑1 (MQ=255)

                       gggATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTg                                                                  >  1:1562054/1‑93 (MQ=255)

                         gATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGAt                                                                <  2:7234930/93‑1 (MQ=255)

                          aTCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGAtt                                                               <  1:1840136/93‑1 (MQ=255)

                             aaaaGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTc                                                            <  1:5441857/93‑1 (MQ=255)

                             aaaaGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTc                                                            >  2:1460898/1‑93 (MQ=255)

                             aaaaGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTc                                                            >  2:441728/1‑93 (MQ=255)

                                aGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGAt                                                         >  1:8160217/1‑93 (MQ=255)

                                 gTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGAtt                                                        >  2:6520003/1‑93 (MQ=255)

                                 atAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGAtt                                                        <  1:6743666/92‑1 (MQ=255)

                                 atAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGAtt                                                        <  1:7136443/92‑1 (MQ=255)

                                  tAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTg                                                       <  2:2080276/93‑1 (MQ=255)

                                   aaaaaGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGc                                                      >  1:1886320/1‑93 (MQ=255)

                                   aaaaaGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCAGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGc                                                      >  1:8091695/1‑93 (MQ=255)

                                    aaaaGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGcc                                                     <  2:7642828/93‑1 (MQ=255)

                                     aaaGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGccc                                                    >  1:6915206/1‑93 (MQ=255)

                                     aaaGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGccc                                                    >  1:7178400/1‑93 (MQ=255)

                                      aaGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCg                                                   >  1:6394609/1‑93 (MQ=255)

                                      aaGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCg                                                   >  2:8025054/1‑93 (MQ=255)

                                      aaGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCg                                                   >  1:3913271/1‑93 (MQ=255)

                                       aGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCCACTTGATTGTCGCTTGCCCgg                                                  >  2:8178708/1‑93 (MQ=255)

                                        gATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGa                                                 >  2:3688138/1‑93 (MQ=255)

                                        gATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGa                                                 >  2:7451221/1‑93 (MQ=255)

                                        gATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGa                                                 >  2:7068186/1‑93 (MQ=255)

                                        gATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGa                                                 >  2:1844129/1‑93 (MQ=255)

                                         aTTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGaa                                                >  2:5569926/1‑93 (MQ=255)

                                         aTTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGaa                                                >  1:3502784/1‑93 (MQ=255)

                                          ttCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGaaa                                               <  2:6145130/93‑1 (MQ=255)

                                             gTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTc                                            >  1:8357217/1‑93 (MQ=255)

                                             gTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTc                                            >  1:7662790/1‑93 (MQ=255)

                                              tGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCa                                           <  1:441728/93‑1 (MQ=255)

                                               gcgcgcAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAg                                          <  2:1416639/93‑1 (MQ=255)

                                                cgcgcAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGc                                         <  2:3595279/93‑1 (MQ=255)

                                                cgcgcAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGc                                         <  2:5891248/93‑1 (MQ=255)

                                                cgcgcAGTGCACTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGc                                         <  2:1092383/93‑1 (MQ=255)

                                                 gcgcAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCg                                        >  1:1466182/1‑93 (MQ=255)

                                                   gcAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTc                                      <  2:6658691/93‑1 (MQ=255)

                                                   gcAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTc                                      >  2:3048852/1‑93 (MQ=255)

                                                   gcAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTc                                      <  2:5813239/93‑1 (MQ=255)

                                                    cAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCt                                     >  1:6620844/1‑93 (MQ=255)

                                                     aGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTg                                    >  1:6333030/1‑93 (MQ=255)

                                                      gTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTgg                                   >  2:1176256/1‑93 (MQ=255)

                                                      gTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTgg                                   >  2:8592766/1‑93 (MQ=255)

                                                              cGCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCAt                           >  2:2016298/1‑93 (MQ=255)

                                                               gCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATc                          <  1:4032547/93‑1 (MQ=255)

                                                               gCCCATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATc                          <  1:6542778/93‑1 (MQ=255)

                                                                cccATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATcc                         <  1:7509905/93‑1 (MQ=255)

                                                                cccATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATcc                         >  1:619350/1‑93 (MQ=255)

                                                                 ccATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTTCCCGGAAATTCAGCGTCTGGATATTCATCCt                        >  1:1058603/1‑93 (MQ=255)

                                                                 ccATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCt                        >  2:4135070/1‑93 (MQ=255)

                                                                 ccATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCt                        >  1:3737274/1‑93 (MQ=255)

                                                                  cATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTTATCCtt                       >  2:7759274/1‑93 (MQ=255)

                                                                  cATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCtt                       >  2:3996277/1‑93 (MQ=255)

                                                                  cATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCtt                       <  2:8381035/93‑1 (MQ=255)

                                                                  cATTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCtt                       <  1:5080197/93‑1 (MQ=255)

                                                                  cATTGGATGGTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCtt                       <  1:7350668/93‑1 (MQ=255)

                                                                   aTTGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCttt                      >  1:1695583/1‑93 (MQ=255)

                                                                     tGGATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGc                    <  2:6992261/93‑1 (MQ=255)

                                                                       gATGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTg                  >  2:1251342/1‑93 (MQ=255)

                                                                        aTGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTgg                 >  1:6035133/1‑93 (MQ=255)

                                                                        aTGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTgg                 >  1:7996809/1‑93 (MQ=255)

                                                                        aTGTTGCAGGCTTGAGCCGGCTTATGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTgg                 <  2:4822611/93‑1 (MQ=255)

                                                                         tGTTGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGc                <  1:2380500/93‑1 (MQ=255)

                                                                         tGTTGCAGGCTTGAGCCGGCTTCT‑GTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCt               >  2:8766057/1‑93 (MQ=255)

                                                                            tGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTc             <  1:8581590/93‑1 (MQ=255)

                                                                            tGCAGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTc             <  2:2171721/93‑1 (MQ=255)

                                                                               aGGCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTTGCTTCTgg          >  2:5969748/1‑93 (MQ=255)

                                                                                ggCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTCTGGc         >  1:2845592/1‑93 (MQ=255)

                                                                                 gCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTCTGGCa        <  2:7267951/93‑1 (MQ=255)

                                                                                 gCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTCTGGCa        <  1:3240100/93‑1 (MQ=255)

                                                                                 gCTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTCTGGCa        <  1:8288429/93‑1 (MQ=255)

                                                                                  cTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTCTGGCAg       <  1:4345517/93‑1 (MQ=255)

                                                                                  cTTGAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTCTGGCAg       >  1:5955224/1‑93 (MQ=255)

                                                                                     gAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTCTGGCAGTGa    >  2:5074016/1‑93 (MQ=255)

                                                                                     gAGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTCTGGCAGTGa    >  1:8784195/1‑93 (MQ=255)

                                                                                      aGCCGGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTCTGGCAGTGaa   <  1:4667411/93‑1 (MQ=255)

TGGCCCGCTATCTGGTTATTCAGGGGATCAAAAGTAAAAAGATTCGTGCGCGCAGTGCGCTACGCCCATTGGATGTTGCAGGCTTGAGCCAGCTTCTGGTGCAGGTTTCCAACTTGATTGTCGATTGCCCGGAAATTCAGCGTCTGGATATTCATCCTTTGCTGGCTTCTGGCAGTGAAT  >  NC_000913/2719846‑2720025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`