BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,751,091	G→C	A426P (GCT→CCT)	recN →	RecN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,751,091	0	G	C	99.6%	942.2 / NA	271	A426P (GCT→CCT)	recN	RecN
Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (145/125); minor base T (1/0); total (146/125)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 279 total aligned reads displayed.

GATGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGGCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAACCCCGGCA  >  NC_000913/2751001‑2751180

 atGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTa                                                                                        >  1:1276939/1‑93 (MQ=255)

  tGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTAt                                                                                       <  1:4805309/93‑1 (MQ=255)

  tGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTAt                                                                                       >  2:4874068/1‑93 (MQ=255)

  tGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTAt                                                                                       <  1:4191761/93‑1 (MQ=255)

  tGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTAt                                                                                       >  2:53947/1‑93 (MQ=255)

    ttAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTg                                                                                     >  2:8744914/1‑93 (MQ=255)

     tAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGc                                                                                    >  2:2290517/1‑93 (MQ=255)

         tttGACGAGCATCACCTGGGCTCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCaaa                                                                                <  1:4096425/93‑1 (MQ=255)

         tttGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCaaa                                                                                <  2:1500627/93‑1 (MQ=255)

          ttGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAg                                                                               <  2:3170248/93‑1 (MQ=255)

          ttGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAg                                                                               <  2:6803460/93‑1 (MQ=255)

          ttGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAg                                                                               <  2:6354839/93‑1 (MQ=255)

           tGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGt                                                                              <  2:579286/93‑1 (MQ=255)

              cGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGc                                                                           <  1:8363192/93‑1 (MQ=255)

                aGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCAt                                                                         >  1:2676072/1‑93 (MQ=255)

                 gCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATc                                                                        >  2:119817/1‑93 (MQ=255)

                 gCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATc                                                                        >  2:3533311/1‑93 (MQ=255)

                  cATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATcc                                                                       <  1:7144624/93‑1 (MQ=255)

                   aTCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCg                                                                      >  2:7074154/1‑93 (MQ=255)

                   aTCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCg                                                                      >  1:1788670/1‑93 (MQ=255)

                    tCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCgg                                                                     >  2:5281877/1‑93 (MQ=255)

                      aCCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCggtg                                                                   >  2:1453468/1‑93 (MQ=255)

                      aCCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCggtg                                                                   >  2:2476008/1‑93 (MQ=255)

                      aCCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCggtg                                                                   <  1:5384475/93‑1 (MQ=255)

                       ccTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCggtgg                                                                  >  1:2899651/1‑93 (MQ=255)

                        cTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCggtggt                                                                 >  2:4144009/1‑93 (MQ=255)

                        cTGGGCGCTGAAGGAGCCGATCGTATTGAGTTTCGGGTAACCACCCACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCTggcggt                                                                 >  2:2545114/1‑93 (MQ=255)

                          gggCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGa                                                               <  1:5209486/93‑1 (MQ=255)

                          gggCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGa                                                               >  1:6506093/1‑93 (MQ=255)

                          gggCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGa                                                               >  2:2048927/1‑93 (MQ=255)

                          gggCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGa                                                               <  1:757566/93‑1 (MQ=255)

                           ggCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGCCAGCCAATGCAGCCTATTCCAAAAGCCGCACCCGGGGGGGat                                                              >  2:5474675/1‑92 (MQ=255)

                              gcTGACGGTGCCGATCGGATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTg                                                           <  2:1177959/93‑1 (MQ=255)

                                tGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTc                                                         <  2:837150/93‑1 (MQ=255)

                                 gACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTcc                                                        <  1:1558002/93‑1 (MQ=255)

                                 gACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTcc                                                        >  2:5121169/1‑93 (MQ=255)

                                  aCGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTccc                                                       <  2:3972237/93‑1 (MQ=255)

                                  aCGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTccc                                                       <  2:6531783/93‑1 (MQ=255)

                                    ggTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGc                                                     >  2:8246911/1‑93 (MQ=255)

                                     gTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCa                                                    >  1:5856921/1‑93 (MQ=255)

                                     gTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCa                                                    <  2:2245660/93‑1 (MQ=255)

                                      tGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCAt                                                   <  2:1788374/93‑1 (MQ=255)

                                       gCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATc                                                  <  2:4849025/93‑1 (MQ=255)

                                       gCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATc                                                  >  1:1143571/1‑93 (MQ=255)

                                        ccGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCg                                                 <  1:4382853/93‑1 (MQ=255)

                                        ccGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCg                                                 <  1:7013413/93‑1 (MQ=255)

                                        ccGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCg                                                 >  2:2736367/1‑93 (MQ=255)

                                          gATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCa                                               >  2:1545276/1‑93 (MQ=255)

                                           aTCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCAc                                              <  1:7909139/93‑1 (MQ=255)

                                            tCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACt                                             >  2:93813/1‑93 (MQ=255)

                                            tCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACt                                             <  1:4593937/93‑1 (MQ=255)

                                            tCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACt                                             <  2:6132783/93‑1 (MQ=255)

                                                 ttGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCaa                                        <  1:6549115/93‑1 (MQ=255)

                                                 ttGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCaa                                        <  1:6665767/93‑1 (MQ=255)

                                                 ttGAGTTTCGGGGGACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCaa                                        <  2:2831812/93‑1 (MQ=255)

                                                   gAGTTGCGGGTAACCACCATCCCAGGTCAGCCAATGCAGCCTAGTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATc                                      <  1:3049838/93‑1 (MQ=255)

                                                    aGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATcc                                     >  2:1380686/1‑93 (MQ=255)

                                                     gTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCa                                    <  1:2575986/93‑1 (MQ=255)

                                                     gTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCa                                    >  2:8452459/1‑93 (MQ=255)

                                                     gTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGCTGAATTGTCCCGCATCGCACTGGCAATCCa                                    >  1:3140113/1‑93 (MQ=255)

                                                      tttCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAg                                   <  2:2404865/93‑1 (MQ=255)

                                                      tttCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAg                                   <  2:3771912/93‑1 (MQ=255)

                                                        tCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGt                                 >  2:534696/1‑93 (MQ=255)

                                                        tCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGt                                 <  1:3947802/93‑1 (MQ=255)

                                                        tCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGt                                 <  2:8025870/93‑1 (MQ=255)

                                                          gggTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGtca                               >  1:6028343/1‑93 (MQ=255)

                                                           ggTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGtcat                              <  2:4261097/93‑1 (MQ=255)

                                                            gtgacccccAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGtcatc                             <  1:2222223/90‑1 (MQ=255)

                                                            gTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGtcatc                             >  2:7267692/1‑93 (MQ=255)

                                                             tAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGtcatca                            <  2:3689423/93‑1 (MQ=255)

                                                             tAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGtcatca                            <  1:8550091/93‑1 (MQ=255)

                                                              aaCCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCAc                           >  1:2754295/1‑93 (MQ=255)

                                                                 caccaACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGgc                        >  2:4431793/1‑93 (MQ=255)

                                                                 caccaACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGgc                        >  2:4693471/1‑93 (MQ=255)

                                                                 caccaACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGgc                        >  2:3134093/1‑93 (MQ=255)

                                                                  accaACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGgcg                       >  2:3403624/1‑93 (MQ=255)

                                                                     agcccAGGTCAGCAAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGt                    <  1:8046614/91‑1 (MQ=255)

                                                                      aCCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTa                   >  1:7926200/1‑93 (MQ=255)

                                                                        ccAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTaaa                 >  1:4880627/1‑93 (MQ=255)

                                                                         cAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTaaaa                <  2:4580983/93‑1 (MQ=255)

                                                                         cAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTaaaa                >  1:2342898/1‑93 (MQ=255)

                                                                          aGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAAt               <  2:2600836/93‑1 (MQ=255)

                                                                           ggTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATg              >  2:7524396/1‑93 (MQ=255)

                                                                           ggTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATg              >  1:5660710/1‑93 (MQ=255)

                                                                             tCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGa            >  2:1078382/1‑93 (MQ=255)

                                                                             tCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGa            <  1:323266/93‑1 (MQ=255)

                                                                              cAGCCAATGCAGCCTATTGCCAAAGTCGCATGCGGTGGTGAATGGTCCCGCATCGCTCTGGCAATCCAGGTCATCACGGCGCGTAAAATGGaa           <  2:4285313/93‑1 (MQ=255)

                                                                               aGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGaaa          >  2:2062111/1‑93 (MQ=255)

                                                                                gCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAAc         >  1:1742955/1‑93 (MQ=255)

                                                                                gCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAAc         >  1:5267496/1‑93 (MQ=255)

                                                                                 ccAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAAcc        <  1:3347931/93‑1 (MQ=255)

                                                                                  cAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAAccc       <  2:1888783/93‑1 (MQ=255)

                                                                                  cAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAAccc       >  2:5007337/1‑93 (MQ=255)

                                                                                   cctGCAGCCTATTCCCAAAGTCGCATCCGGTGGCGAATTGTCCCGAATCGCAATGGCAATCCAGGACATCACGGCGCGGACAATGGATAcacc      >  2:6295150/3‑93 (MQ=255)

                                                                                   aaTGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAAcccc      >  2:2864038/1‑93 (MQ=255)

                                                                                   aaTGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAAcccc      <  1:1380832/93‑1 (MQ=255)

                                                                                    aTGCAGCCTATTGCCAAAGTCGCATCCTGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAACCCCg     >  2:7678011/1‑93 (MQ=255)

                                                                                    aTGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAACCCCg     <  1:2698510/93‑1 (MQ=255)

                                                                                      gCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAACCTCGGc   >  1:4239978/1‑93 (MQ=255)

                                                                                       cAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAACCCCGGCa  >  1:5594752/1‑93 (MQ=255)

GATGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGGCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAACCCCGGCA  >  NC_000913/2751001‑2751180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`