BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,805,526	A→G	D125G (GAC→GGC)	proX →	high‑affinity transport system for glycine betaine and proline

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,805,526	0	A	G	100.0%	1010.6 / NA	296	D125G (GAC→GGC)	proX	high‑affinity transport system for glycine betaine and proline
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (144/152); total (144/152)

Only 100 of 306 total aligned reads displayed.

AAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAA  >  NC_000913/2805436‑2805615

aaGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAGAACCGCCGGcc                                                                                         >  1:8320303/1‑93 (MQ=255)

aaGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGcc                                                                                         <  2:7601418/93‑1 (MQ=255)

aaGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGcc                                                                                         >  2:3565992/1‑93 (MQ=255)

aaGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACAGCCGGcc                                                                                         >  2:894956/1‑93 (MQ=255)

 aGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCa                                                                                        >  2:6751421/1‑93 (MQ=255)

 aGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCa                                                                                        >  2:4405951/1‑93 (MQ=255)

  gttGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAg                                                                                       <  2:5587875/91‑1 (MQ=255)

    tGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTa                                                                                     >  2:8659475/1‑93 (MQ=255)

    tGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTa                                                                                     <  1:2963943/93‑1 (MQ=255)

     gCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTAc                                                                                    <  1:2648536/93‑1 (MQ=255)

     gCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTAc                                                                                    <  1:1062702/93‑1 (MQ=255)

      ccGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACa                                                                                   >  2:719962/1‑93 (MQ=255)

       cGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACaa                                                                                  >  2:7965256/1‑93 (MQ=255)

        ggTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACaaa                                                                                 <  1:5031776/93‑1 (MQ=255)

        ggTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACaaa                                                                                 >  1:1772699/1‑93 (MQ=255)

         gTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACaaaa                                                                                >  1:5691742/1‑93 (MQ=255)

          tGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTATCTGATCGATAAGAAAACCGCCGGCCAGTACAAAAt                                                                               >  2:8046883/1‑93 (MQ=255)

           ggCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAAta                                                                              >  2:6347728/1‑92 (MQ=255)

            gCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCa                                                                             <  2:5474571/93‑1 (MQ=255)

            gCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCa                                                                             <  2:4160371/93‑1 (MQ=255)

             cGATAAGAAATTTTATCGTGAAGGGGAATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCAc                                                                            >  2:7703063/1‑93 (MQ=255)

              gATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATATGAAAACCGCCGGCCAGTACAAAATCAcc                                                                           >  2:3846859/1‑93 (MQ=255)

              gATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCAcc                                                                           >  2:196112/1‑93 (MQ=255)

              gATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCAcc                                                                           >  1:1729188/1‑93 (MQ=255)

              gATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCAcc                                                                           <  2:5012341/93‑1 (MQ=255)

              gATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCAcc                                                                           <  2:6243390/93‑1 (MQ=255)

               aTAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCa                                                                          >  1:8720792/1‑93 (MQ=255)

                tAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCaa                                                                         >  2:2440078/1‑93 (MQ=255)

                 aaGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAAc                                                                        >  2:8370933/1‑93 (MQ=255)

                 aaGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAAc                                                                        >  2:5667935/1‑93 (MQ=255)

                  aGAAATTTTATCGTGAAGGGGAATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACGAACa                                                                       >  2:6823382/1‑93 (MQ=255)

                   gAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACAt                                                                      <  2:2910788/93‑1 (MQ=255)

                           aTCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAAc                                                              <  1:4946629/93‑1 (MQ=255)

                           aTCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAAc                                                              >  1:2154435/1‑93 (MQ=255)

                            tCGTGAAGGGTGATTTGTTAACGGCGCGGCACAGGGTTCCCTGAGCGATAAGAAAACCGCCGGCCAGTACAAAATCACAAACATCGCACAACt                                                             <  1:6620408/93‑1 (MQ=255)

                             cGTGAAGGGGTATTTGTTAACGGCGCAGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTg                                                            <  1:7684173/93‑1 (MQ=255)

                              gTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGa                                                           >  2:2575543/1‑93 (MQ=255)

                              gTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGa                                                           <  1:1555290/93‑1 (MQ=255)

                                gAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGaaa                                                         <  1:5639108/93‑1 (MQ=255)

                                 aaGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAg                                                        >  2:5828848/1‑93 (MQ=255)

                                 aaGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAg                                                        <  1:7456820/93‑1 (MQ=255)

                                 aaGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAg                                                        <  1:1282514/93‑1 (MQ=255)

                                 aaGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAg                                                        <  1:5182564/93‑1 (MQ=255)

                                  aGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGa                                                       >  1:4555717/1‑93 (MQ=255)

                                  aGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGa                                                       <  1:2185650/93‑1 (MQ=255)

                                  aGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGa                                                       >  1:4410681/1‑93 (MQ=255)

                                  aGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGa                                                       <  1:8102986/93‑1 (MQ=255)

                                       tATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGa                                                  >  1:8526760/1‑93 (MQ=255)

                                       tATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGa                                                  <  2:651972/93‑1 (MQ=255)

                                        ttttGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGaa                                                 <  2:8808111/92‑1 (MQ=255)

                                        aTTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGaa                                                 <  1:6106111/93‑1 (MQ=255)

                                         tttGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAg                                                >  2:3262279/1‑93 (MQ=255)

                                          ttGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGa                                               <  1:5245166/93‑1 (MQ=255)

                                          ttGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGa                                               <  2:4059018/93‑1 (MQ=255)

                                           tGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGAt                                              <  1:2073704/93‑1 (MQ=255)

                                           tGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGAt                                              >  1:2333885/1‑93 (MQ=255)

                                                 cGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCaa                                        >  2:4481909/1‑93 (MQ=255)

                                                     gcgGCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTg                                    >  2:3895299/1‑93 (MQ=255)

                                                       ggCACAGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGtt                                  <  1:6421357/93‑1 (MQ=255)

                                                         cacaGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCg                                <  2:5387048/93‑1 (MQ=255)

                                                          acaGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGa                               >  2:7753758/1‑93 (MQ=255)

                                                           caGGGTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGAt                              <  1:3269477/93‑1 (MQ=255)

                                                             gggTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATAc                            <  2:6681000/93‑1 (MQ=255)

                                                             gggTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATAc                            <  1:5389564/93‑1 (MQ=255)

                                                               gTTACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAAATGAAAGATCCGAAGATCGCCAAACTGTTCGATACCa                          <  2:2919738/93‑1 (MQ=255)

                                                                ttACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCaa                         <  1:1127705/93‑1 (MQ=255)

                                                                ttACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCaa                         >  2:1524635/1‑93 (MQ=255)

                                                                 tACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGTTACTAAc                        >  1:5496453/1‑93 (MQ=255)

                                                                 tACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAAc                        <  1:408346/93‑1 (MQ=255)

                                                                 tACCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAAc                        <  1:4740485/93‑1 (MQ=255)

                                                                  aCCTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCGACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACg                       <  2:6055683/93‑1 (MQ=255)

                                                                   ccTGGTCGATAACAAAACCGGCGGCCAGGACAAAATCACCAAGATCGCACAACTGAAAGATCCGAAGATCGCGAAACTGTTCGATACCAACgg                      <  1:2486492/93‑1 (MQ=255)

                                                                   ccTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACgg                      <  2:1554057/93‑1 (MQ=255)

                                                                    cTGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGc                     <  2:1171200/93‑1 (MQ=255)

                                                                     tGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCg                    >  2:4313041/1‑93 (MQ=255)

                                                                     tGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCg                    >  2:1763961/1‑93 (MQ=255)

                                                                     tGATCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCg                    <  2:101746/93‑1 (MQ=255)

                                                                      gaccGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGa                   <  2:2360443/90‑1 (MQ=255)

                                                                        tCGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACg                 >  2:8416166/1‑93 (MQ=255)

                                                                         cGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATCCCAACGGCgcggg                >  1:1871436/1‑89 (MQ=255)

                                                                         cGATAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACgg                <  1:3450648/93‑1 (MQ=255)

                                                                          gATAAAAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGa               <  1:6900377/93‑1 (MQ=255)

                                                                           aTAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGaa              >  1:7519845/1‑93 (MQ=255)

                                                                            tAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGaaa             >  1:7393051/1‑93 (MQ=255)

                                                                            tAAGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGaaa             >  1:7098275/1‑93 (MQ=255)

                                                                             aaGAAACCCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGaaaa            <  2:642167/93‑1 (MQ=255)

                                                                             aaGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGaaaa            <  1:4827385/93‑1 (MQ=255)

                                                                              aGAAACCCCCCGGCCAGTACAAAAGCACCATCATCGGACAACGGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAg           <  1:4190683/93‑1 (MQ=255)

                                                                              aGAAAACCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAg           <  1:1835692/93‑1 (MQ=255)

                                                                                aaaaCCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCg         <  1:2828869/93‑1 (MQ=255)

                                                                                  aaCCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGa       <  1:3401152/93‑1 (MQ=255)

                                                                                  aaCCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGa       <  2:2205053/93‑1 (MQ=255)

                                                                                  aaCCGCCGGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGa       <  1:2273450/93‑1 (MQ=255)

                                                                                   aCCGCCGGCCAGTACAAAATCGCCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGAt      <  1:3633219/93‑1 (MQ=255)

                                                                                    ccgccgGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGAtt     >  2:8316567/1‑93 (MQ=255)

                                                                                     cgccgGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGAttt    <  2:3046497/93‑1 (MQ=255)

                                                                                      gccgGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTa   >  1:3116517/1‑93 (MQ=255)

                                                                                      gccgGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTa   >  2:8437514/1‑93 (MQ=255)

                                                                                       ccgGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTaa  >  2:4929416/1‑93 (MQ=255)

                                                                                       ccgGCCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTaa  <  1:7854670/93‑1 (MQ=255)

AAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAACCGCCGACCAGTACAAAATCACCAACATCGCACAACTGAAAGATCCGAAGATCGCCAAACTGTTCGATACCAACGGCGACGGAAAAGCGGATTTAA  >  NC_000913/2805436‑2805615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`