BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,819,446	G→C	G196G (GGC→GGG)	alaS ←	alanyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,819,446	0	G	C	99.7%	994.1 / ‑4.7	295	G196G (GGC→GGG)	alaS	alanyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base G (1/0); new base C (169/124); total (170/125)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.29e-01

Only 100 of 318 total aligned reads displayed.

CGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGA  >  NC_000913/2819356‑2819535

  tGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGTAGGCcccc                                                                                       >  1:3232326/1‑93 (MQ=255)

   gCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGcccccc                                                                                      >  1:8396596/1‑93 (MQ=255)

    ccATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGTGGCcccccc                                                                                     >  2:3101121/1‑93 (MQ=255)

     cATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGcccccccc                                                                                    >  2:6967948/1‑93 (MQ=255)

      aTCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCa                                                                                   >  1:583699/1‑93 (MQ=255)

      aTCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCa                                                                                   >  2:4383569/1‑93 (MQ=255)

       tCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCaa                                                                                  >  2:7716257/1‑93 (MQ=255)

       tCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCaa                                                                                  >  1:4205873/1‑93 (MQ=255)

        cGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCaaa                                                                                 <  2:2254030/93‑1 (MQ=255)

           ccTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAAtgt                                                                              <  2:5224317/93‑1 (MQ=255)

            cTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAAtgtg                                                                             >  2:2140060/1‑93 (MQ=255)

             tGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTgg                                                                            >  1:6045875/1‑93 (MQ=255)

             tGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTgg                                                                            >  1:7932700/1‑93 (MQ=255)

                 ggTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGc                                                                        <  1:3114145/93‑1 (MQ=255)

                 ggTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGc                                                                        <  1:5767437/93‑1 (MQ=255)

                  gTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCCAATGTGGTCGcc                                                                       >  2:4106199/1‑93 (MQ=255)

                  gTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGcc                                                                       >  2:7612860/1‑93 (MQ=255)

                   ttGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCg                                                                      >  2:3677322/1‑93 (MQ=255)

                   ttGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCg                                                                      >  2:2496060/1‑93 (MQ=255)

                    tGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGt                                                                     <  2:5364119/93‑1 (MQ=255)

                      aaCTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGa                                                                   <  1:4692752/93‑1 (MQ=255)

                      aaCTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGa                                                                   <  2:1123638/93‑1 (MQ=255)

                       aCTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGAt                                                                  >  2:2937367/1‑93 (MQ=255)

                       aCTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGAt                                                                  >  2:4837491/1‑93 (MQ=255)

                        cTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATc                                                                 >  1:7347437/1‑93 (MQ=255)

                        cTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATc                                                                 >  2:6027797/1‑93 (MQ=255)

                        cTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATc                                                                 >  1:1007159/1‑93 (MQ=255)

                         tGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCg                                                                >  1:4386476/1‑93 (MQ=255)

                          gCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGt                                                               >  1:5452129/1‑93 (MQ=255)

                          gCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGt                                                               >  2:7956798/1‑93 (MQ=255)

                           cATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTa                                                              >  2:131491/1‑93 (MQ=255)

                            aTGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTag                                                             >  1:987754/1‑93 (MQ=255)

                             tGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGGGGGCGCCGGGATGGAaga                                                            >  1:2556638/1‑93 (MQ=255)

                               aaGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTagaag                                                          >  1:1249627/1‑93 (MQ=255)

                               aaGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTagaag                                                          <  1:354728/93‑1 (MQ=255)

                                aGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTagaaga                                                         >  1:6855772/1‑93 (MQ=255)

                                aGACCATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTagaaga                                                         <  2:7320334/93‑1 (MQ=255)

                                 gACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGAt                                                        <  2:4962634/93‑1 (MQ=255)

                                 gACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGAt                                                        >  1:6946636/1‑93 (MQ=255)

                                 gACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGAt                                                        >  2:4294058/1‑93 (MQ=255)

                                 gACGATGTTCCAGATAACAATGAAACGGACGCCGTCTTCTTCCGGACTTCCCGGAGGCCCACCCCAAATGTGGACGCCGTGAACGTagaagaa                                                        <  1:7845229/93‑2 (MQ=255)

                                  aCGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGAtt                                                       >  1:1702092/1‑93 (MQ=255)

                                    gATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTc                                                     <  1:4418782/93‑1 (MQ=255)

                                     aTGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCg                                                    <  1:2660413/93‑1 (MQ=255)

                                     aTGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCg                                                    <  1:3945857/93‑1 (MQ=255)

                                      tGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCgg                                                   >  1:4945513/1‑93 (MQ=255)

                                        ttCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTg                                                 >  2:7492684/1‑93 (MQ=255)

                                         tCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGc                                                <  1:6206420/93‑1 (MQ=255)

                                          ccAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCa                                               <  1:8645412/93‑1 (MQ=255)

                                           cAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCAc                                              >  2:662221/1‑93 (MQ=255)

                                             gATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACgg                                            >  1:6508059/1‑93 (MQ=255)

                                              aTCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACggg                                           >  1:8505411/1‑93 (MQ=255)

                                               tctcAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGc                                          >  2:3270805/1‑93 (MQ=255)

                                               tctcAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGc                                          >  1:7217502/1‑93 (MQ=255)

                                                ctcAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGcg                                         >  1:1937039/1‑92 (MQ=255)

                                                ctcAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGcc                                         >  2:1747110/1‑93 (MQ=255)

                                                ctcAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGACCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGcc                                         <  2:8229714/93‑1 (MQ=255)

                                                 tcAATGTAGCGGTCGCGGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCg                                        <  2:5140828/93‑1 (MQ=255)

                                                 tcAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCg                                        >  1:8069718/1‑93 (MQ=255)

                                                 tcAATGTAGCGGTCGCCGTCTTCTGCCGGTCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCg                                        <  1:5349431/93‑1 (MQ=255)

                                                   aaTGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGCCCCCCCCCAAATGTGGTCGCCGGGATCGTAGAAGATTTCGGTGCCCGGGCCGCa                                      >  1:7508825/1‑93 (MQ=255)

                                                     tGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACg                                    <  2:3875278/93‑1 (MQ=255)

                                                     tGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACg                                    <  2:6526118/93‑1 (MQ=255)

                                                        aGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGAc                                 <  2:6733919/93‑1 (MQ=255)

                                                        aGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGAc                                 >  1:1471681/1‑93 (MQ=255)

                                                           ggTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAg                              <  1:3903157/93‑1 (MQ=255)

                                                           ggTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAg                              <  1:4499689/93‑1 (MQ=255)

                                                             gcGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAgtg                            <  1:2231988/92‑1 (MQ=255)

                                                               gCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTc                          >  1:280189/1‑93 (MQ=255)

                                                                ccGTCCTCTTCCGGGCTGCCCGGAGCCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCa                         <  2:5022751/93‑1 (MQ=255)

                                                                 cGTCTTCTTCTGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCTTGATCGTAGAAGATTTCGGTGCACGGTCCTCACGGACCAGTGTCAc                        <  2:5067993/93‑1 (MQ=255)

                                                                  gTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGGCGCACGGACCAGTGTCAcc                       >  1:7742093/1‑93 (MQ=255)

                                                                  gTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCAcc                       >  2:3317792/1‑93 (MQ=255)

                                                                  gTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCAcc                       >  1:8768400/1‑93 (MQ=255)

                                                                  ctcttctTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCAcc                       <  2:2637608/92‑1 (MQ=255)

                                                                   tcttctTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCAccc                      >  1:8706396/1‑93 (MQ=255)

                                                                    ctccttcCGGGCTTCCCGGAGCCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCa                     <  2:2049814/90‑1 (MQ=255)

                                                                     ttcttcCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCAt                    >  2:8369594/1‑93 (MQ=255)

                                                                      tcttcCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATc                   <  2:4488875/93‑1 (MQ=255)

                                                                      tcttcCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATc                   <  1:5057061/93‑1 (MQ=255)

                                                                      tcttcCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATc                   <  1:4332667/93‑1 (MQ=255)

                                                                      tcttcCGGGCTTCCCGGAGGCCCCCCCCAAATGCGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATc                   >  2:6385613/1‑93 (MQ=255)

                                                                      tcttcCGGGCTGCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATc                   <  2:193455/93‑1 (MQ=255)

                                                                         tcCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGc                <  1:1368171/93‑1 (MQ=255)

                                                                           cGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGGCa              <  1:373575/93‑1 (MQ=255)

                                                                            gggCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAg             >  1:2108402/1‑93 (MQ=255)

                                                                            gggCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAg             >  2:8501276/1‑93 (MQ=255)

                                                                            gggCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAg             >  2:579302/1‑93 (MQ=255)

                                                                             ggCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGa            <  2:2839682/93‑1 (MQ=255)

                                                                             ggCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACACATCTGCCAGa            >  2:1399616/1‑93 (MQ=255)

                                                                              gCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGaa           >  2:5625728/1‑93 (MQ=255)

                                                                              gCTTCCAGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGaa           >  2:8553722/1‑93 (MQ=255)

                                                                                ttCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGt         >  2:1487366/1‑93 (MQ=255)

                                                                                ttCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGt         >  1:1585306/1‑93 (MQ=255)

                                                                                   ccGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGt      <  2:5962840/93‑1 (MQ=255)

                                                                                   ccGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGt      >  1:674302/1‑93 (MQ=255)

                                                                                    cGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTc     >  1:3468732/1‑93 (MQ=255)

                                                                                     ggAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCa    <  2:7877683/93‑1 (MQ=255)

                                                                                     ggAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCa    <  1:3300194/93‑1 (MQ=255)

                                                                                       aGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGa  <  2:882572/93‑1 (MQ=255)

CGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGA  >  NC_000913/2819356‑2819535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`