Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 254,298 T→C T473T (ACA→ACG)  pepD ← aminoacyl‑histidine dipeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913254,2980TC99.3% 1003.7 / ‑3.4 293T473T (ACA→ACG) pepDaminoacyl‑histidine dipeptidase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (141/150);  total (142/151)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

Only 100 of 303 total aligned reads displayed.

GATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCAT  >  NC_000913/254208‑254387
                                                                                          |                                                                                         
   gAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCa                                                                                      <  1:6786397/93‑1 (MQ=255)
    aaaCTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAg                                                                                     >  1:1833642/1‑93 (MQ=255)
    aaaCTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAg                                                                                     <  2:2379987/93‑1 (MQ=255)
     aaCTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGt                                                                                    >  1:6106813/1‑93 (MQ=255)
      agcgCCGGAAGGAGATTAAAAGCAAGCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTa                                                                                   <  1:7526495/90‑1 (MQ=255)
       cTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTaa                                                                                  <  2:7662703/93‑1 (MQ=255)
         gCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATg                                                                                >  1:8410979/1‑93 (MQ=255)
          ccGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGa                                                                               <  1:4896165/93‑1 (MQ=255)
           cGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGAc                                                                              <  2:2970935/93‑1 (MQ=255)
            ggAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGAcc                                                                             >  1:1017288/1‑93 (MQ=255)
            ggAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGAcc                                                                             >  2:2159953/1‑93 (MQ=255)
            ggAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGAcc                                                                             >  2:3678390/1‑93 (MQ=255)
             gAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCt                                                                            >  2:7952077/1‑93 (MQ=255)
                ggCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACg                                                                         >  1:6834031/1‑93 (MQ=255)
                 gCGACTAAAGCCCATCCGGCCGCAAATCAAATTATTCCTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGc                                                                        <  2:7296278/93‑1 (MQ=255)
                  cGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCt                                                                       <  1:1461030/93‑1 (MQ=255)
                   gATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCtt                                                                      >  2:137336/1‑93 (MQ=255)
                   gATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCtt                                                                      >  1:5701264/1‑93 (MQ=255)
                    aTTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCttt                                                                     >  1:3570222/1‑93 (MQ=255)
                    aTTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCttt                                                                     >  2:3658581/1‑93 (MQ=255)
                    aTTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCttt                                                                     <  2:1883758/93‑1 (MQ=255)
                     ttAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTc                                                                    >  1:8519299/1‑93 (MQ=255)
                        aaCGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGAt                                                                 <  1:2103810/93‑1 (MQ=255)
                         aCGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCTGCGTCCAGTAATGACCTACGCTTTCGAtg                                                                <  1:8314411/93‑1 (MQ=255)
                          cGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGAtgt                                                               <  1:4132991/93‑1 (MQ=255)
                            ccATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGa                                                             <  1:2017181/93‑1 (MQ=255)
                            ccATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGa                                                             <  2:1776834/93‑1 (MQ=255)
                             cATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGaa                                                            <  2:6584026/93‑1 (MQ=255)
                              aTCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAAc                                                           <  1:1258241/93‑1 (MQ=255)
                               tCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACt                                                          <  2:6922042/93‑1 (MQ=255)
                               tCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACt                                                          >  2:2464580/1‑93 (MQ=255)
                               accGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACt                                                          <  1:534757/92‑1 (MQ=255)
                                    cagcaAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGACCTTGCTc                                                     >  2:5824129/1‑93 (MQ=255)
                                      gcaAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTTCGCTTTCGATGTGAACTTGCTCAt                                                   <  1:3773804/93‑1 (MQ=255)
                                       caaatcaaatAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATc                                                  <  2:4456486/93‑1 (MQ=255)
                                        aaatcaaatAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATcc                                                 >  2:524402/1‑93 (MQ=255)
                                        aaatcaaatAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATcc                                                 >  2:3334839/1‑93 (MQ=255)
                                        aaatcaaatAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATcc                                                 >  1:5502980/1‑93 (MQ=255)
                                         aatcaaatAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCg                                                >  2:381650/1‑93 (MQ=255)
                                           tcaaatAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGga                                              >  1:235662/1‑93 (MQ=255)
                                           tcaaatAATTACTTCGCCGGAATTTCTTTCAGCAGGTCAGTCAGCAGCGTCCAGTAATGACCTATGCTTTCGATGTGCACTTGCTCATCCGga                                              >  2:5354899/1‑93 (MQ=255)
                                           acaaatAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGga                                              <  1:1783926/92‑1 (MQ=255)
                                             aaatAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGgaga                                            <  1:6743394/93‑1 (MQ=255)
                                             aaatAATTACTTCGCCGGAATTTATTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGgaga                                            <  2:4798855/93‑1 (MQ=255)
                                              aataatTACTTTGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTACGATGTGAACTTGCTCATCCGGagag                                           <  2:2072442/93‑1 (MQ=255)
                                              aataatTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGagag                                           >  1:978243/1‑93 (MQ=255)
                                              aataatTACTTCCCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGagag                                           <  1:6679196/93‑1 (MQ=255)
                                               ataatTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAgt                                          <  1:3207416/93‑1 (MQ=255)
                                                 aatTACTTCGCAGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAgtgt                                        <  1:510161/93‑1 (MQ=255)
                                                   ttACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTgg                                      <  2:2674487/93‑1 (MQ=255)
                                                    tACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGa                                     <  2:2729587/93‑1 (MQ=255)
                                                     tcTTCGCCGGAATTGCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGAc                                    <  1:3442452/92‑1 (MQ=255)
                                                     aCTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGAc                                    >  2:6083744/1‑93 (MQ=255)
                                                     aCTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGGGTGGAc                                    >  1:589824/1‑93 (MQ=255)
                                                      cTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGAcc                                   <  2:1586510/93‑1 (MQ=255)
                                                      cTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGAcc                                   <  2:4078475/93‑1 (MQ=255)
                                                       ttCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCg                                  >  1:4328948/1‑93 (MQ=255)
                                                       ttCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCg                                  <  1:8158870/93‑1 (MQ=255)
                                                        tCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCgg                                 >  2:7622241/1‑93 (MQ=255)
                                                          ggcGGAAATTCTTTCAGCAGTTCAGATTGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGATCTTGCTCATCCGGAGAGTGTGGACCGGTg                               <  2:6756169/91‑1 (MQ=255)
                                                          gCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTg                               >  1:6549703/1‑93 (MQ=255)
                                                          gCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTg                               >  1:2670163/1‑93 (MQ=255)
                                                           cccgAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCCACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGa                              <  1:594206/90‑1 (MQ=255)
                                                           ccGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGa                              <  1:7369312/93‑1 (MQ=255)
                                                           ccGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGa                              <  1:2740454/93‑1 (MQ=255)
                                                           ccGGAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGa                              >  1:4962883/1‑93 (MQ=255)
                                                             ggATTTTGTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTGCGCTTTCGATGTGAACTGGCTCATCCGGAGAGTGTGGACCGGTGATa                            <  1:5785139/93‑1 (MQ=255)
                                                             ggAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATa                            >  1:8615767/1‑93 (MQ=255)
                                                              gAATTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAg                           >  1:5882755/1‑93 (MQ=255)
                                                               aaTTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGt                          <  2:3731238/93‑1 (MQ=255)
                                                                aTTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGtt                         >  2:4384288/1‑93 (MQ=255)
                                                                aTTTCTTTCAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGtt                         >  2:8741263/1‑93 (MQ=255)
                                                                 tttctttcAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTg                        <  2:5143807/93‑1 (MQ=255)
                                                                 tttctttcAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTg                        <  1:7005787/93‑1 (MQ=255)
                                                                 ttgctttcAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTg                        <  1:4722718/90‑1 (MQ=255)
                                                                  ttctttcAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTgg                       >  1:7861607/1‑93 (MQ=255)
                                                                     tttcAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGccc                    <  2:7181199/93‑1 (MQ=255)
                                                                     tttcAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGccc                    >  1:6276336/1‑93 (MQ=255)
                                                                      ttcAGCAGTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTCTGGACCGGTGATAGTTGGCCCg                   >  1:2948424/1‑93 (MQ=255)
                                                                        cagcagTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGAt                 <  2:1134797/93‑1 (MQ=255)
                                                                         agcagTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGATTGTGGACCGGTGATAGTTGGCCCGATa                <  2:5575155/93‑1 (MQ=255)
                                                                         agcagTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATa                >  1:5582348/1‑93 (MQ=255)
                                                                         agcagTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATa                <  1:7971256/93‑1 (MQ=255)
                                                                          gcagTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAg               <  2:4005678/93‑1 (MQ=255)
                                                                          gcagTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAg               <  2:8240705/93‑1 (MQ=255)
                                                                          gcagTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAg               >  2:6351977/1‑93 (MQ=255)
                                                                          gcagTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAg               <  2:5989867/93‑1 (MQ=255)
                                                                           cagTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGa              <  2:4965117/93‑1 (MQ=255)
                                                                            agTTCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGaa             <  2:7762821/93‑1 (MQ=255)
                                                                              ttCAGTCAGCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAAc           <  2:8536875/93‑1 (MQ=255)
                                                                               tcagtcagCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAAcc          >  2:2253755/1‑93 (MQ=255)
                                                                                 agtcagCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCAt        <  2:2820226/93‑1 (MQ=255)
                                                                                  gtcagCAGCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATg       <  1:823979/93‑1 (MQ=255)
                                                                                  gtcagCAGCGTCCAGTAATGACCTACGCTGTCGATGTGAACTTGCTCATCCGGAGATTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATg       <  1:1186417/93‑1 (MQ=255)
                                                                                    cagcagCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTc     <  1:4420344/93‑1 (MQ=255)
                                                                                    cagcagCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTc     >  1:3652202/1‑93 (MQ=255)
                                                                                    cagcagCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGATGGCCCGATAGAAACCATGTc     >  1:8102294/1‑93 (MQ=255)
                                                                                     agcagcGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGtac    >  2:3251451/1‑91 (MQ=255)
                                                                                     agcagcGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTcc    <  2:462780/93‑1 (MQ=255)
                                                                                       cagCGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCAt  <  1:6021609/93‑1 (MQ=255)
                                                                                          |                                                                                         
GATGAAACTGCCGGAAGGCGATTAAACGCCATCCGGCAGCAAATCAAATAATTACTTCGCCGGAATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCAT  >  NC_000913/254208‑254387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑