BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,824,797	T→C	F128S (TTT→TCT)	srlE →	PTS system glucitol/sorbitol‑specific enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,824,797	0	T	C	100.0%	1068.3 / NA	310	F128S (TTT→TCT)	srlE	PTS system glucitol/sorbitol‑specific enzyme IIBC component
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (183/127); total (183/127)

Only 100 of 323 total aligned reads displayed.

CGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTTTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCA  >  NC_000913/2824707‑2824886

cgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCtctt                                                                                         <  1:3358772/93‑1 (MQ=255)

cgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCtctt                                                                                         >  1:8587584/1‑93 (MQ=255)

 gCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTc                                                                                        >  2:3726921/1‑93 (MQ=255)

  cTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTcc                                                                                       >  1:790188/1‑93 (MQ=255)

  cTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTcc                                                                                       >  1:7210619/1‑93 (MQ=255)

   tGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCg                                                                                      >  2:3109181/1‑93 (MQ=255)

     gcgcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTg                                                                                    >  1:639139/1‑93 (MQ=255)

       gcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTggg                                                                                  >  2:8529295/1‑93 (MQ=255)

       gcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTggg                                                                                  <  2:4402624/93‑1 (MQ=255)

       gcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTggg                                                                                  >  2:6371000/1‑93 (MQ=255)

        caatACATTGTGGAAGATATTTATGTCTCTTGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGc                                                                                 <  2:8651266/90‑1 (MQ=255)

          gTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCg                                                                               >  2:6301587/1‑93 (MQ=255)

          gTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCg                                                                               >  2:4556360/1‑93 (MQ=255)

          gTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCg                                                                               <  2:4078519/93‑1 (MQ=255)

            aCATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTg                                                                             <  2:3040223/93‑1 (MQ=255)

            aCATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTg                                                                             >  2:3988195/1‑93 (MQ=255)

              aTTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACACCCCTCTTCCGTGGGCCGTGAc                                                                           <  2:1191721/93‑1 (MQ=255)

              aTTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGAc                                                                           >  1:8455724/1‑93 (MQ=255)

              aTTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGAc                                                                           <  2:7863189/93‑1 (MQ=255)

               ttGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACt                                                                          >  2:2776035/1‑93 (MQ=255)

                tgtgGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTa                                                                         <  1:8186208/93‑1 (MQ=255)

                tgtgGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTa                                                                         <  2:2783628/93‑1 (MQ=255)

                 gtgGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTAt                                                                        <  2:7023685/93‑1 (MQ=255)

                  tgGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATg                                                                       >  1:4671676/1‑93 (MQ=255)

                    gAAGATATTTATGTCTCTGGTGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGac                                                                     <  2:210766/93‑1 (MQ=255)

                    gAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGac                                                                     >  1:6021951/1‑93 (MQ=255)

                      aGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGacac                                                                   >  2:6246567/1‑93 (MQ=255)

                        atatTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCa                                                                 <  1:7557778/93‑1 (MQ=255)

                        atatTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCa                                                                 <  2:5444068/93‑1 (MQ=255)

                          atTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGt                                                               >  1:7034842/1‑93 (MQ=255)

                           tttATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTa                                                              >  1:2045452/1‑93 (MQ=255)

                            ttATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTaa                                                             <  1:3736476/93‑1 (MQ=255)

                             tATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTaaa                                                            >  2:2371197/1‑92 (MQ=255)

                               tGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGaa                                                          >  1:8484831/1‑93 (MQ=255)

                                 tctcTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGaaaa                                                        <  1:5506049/93‑1 (MQ=255)

                                 tctcTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGaaaa                                                        >  1:5293771/1‑93 (MQ=255)

                                 tctcTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACAACAACCCTCTTCCGTGGGCCGTCACTATGACACCAGTAAGaaaa                                                        >  2:6469597/1‑93 (MQ=255)

                                  ctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAAt                                                       >  1:5594419/1‑93 (MQ=255)

                                  ctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAAt                                                       >  1:2083515/1‑93 (MQ=255)

                                  ctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAAt                                                       >  1:360766/1‑93 (MQ=255)

                                  ctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAAt                                                       <  1:7776086/93‑1 (MQ=255)

                                   tctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATc                                                      >  1:8044641/1‑93 (MQ=255)

                                   tctGGCGCAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATc                                                      >  1:7448237/1‑93 (MQ=255)

                                     tGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCAc                                                    <  1:1595894/93‑1 (MQ=255)

                                       gCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCg                                                  <  2:2292742/93‑1 (MQ=255)

                                       gCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCg                                                  >  1:3553341/1‑93 (MQ=255)

                                         gTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGaa                                                >  1:4870504/1‑93 (MQ=255)

                                         gTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGCCCGTGACTATGACACCAGTAAGAAAATCAcaaaa                                                >  1:6555495/1‑89 (MQ=255)

                                          tAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGaaa                                               >  1:1209814/1‑92 (MQ=255)

                                          tAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAAc                                               >  1:2456860/1‑93 (MQ=255)

                                           aaaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACa                                              >  2:5814076/1‑93 (MQ=255)

                                           aaaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACa                                              >  1:230647/1‑93 (MQ=255)

                                            aaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaa                                             >  2:5138245/1‑93 (MQ=255)

                                            aaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaa                                             >  1:1304247/1‑93 (MQ=255)

                                            aaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaa                                             >  1:3910155/1‑93 (MQ=255)

                                            aaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaa                                             >  2:6079248/1‑93 (MQ=255)

                                            aaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaa                                             >  2:8005985/1‑93 (MQ=255)

                                             aagaagAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaaa                                            >  1:2962267/1‑93 (MQ=255)

                                               gaagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGc                                          >  1:3198382/1‑93 (MQ=255)

                                                aagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCg                                         >  2:193373/1‑93 (MQ=255)

                                                 agaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGa                                        >  2:136625/1‑93 (MQ=255)

                                                  gaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGAt                                       <  2:8099946/93‑1 (MQ=255)

                                                   aaaaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCCCCGAACAAAGCGATg                                      <  2:7324051/93‑1 (MQ=255)

                                                     aaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGt                                    >  1:5068927/1‑93 (MQ=255)

                                                     aaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGt                                    <  1:4346459/93‑1 (MQ=255)

                                                     aaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGt                                    >  2:8242774/1‑93 (MQ=255)

                                                      aCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCATTAAGAAAATCACCGAACAAAGCGATGGtt                                   <  1:6253776/93‑1 (MQ=255)

                                                      aCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGtt                                   >  2:7104396/1‑93 (MQ=255)

                                                      aCATCACTGCAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGCGACTATGACACCAGTAAGAAAATCATCGAACAAAGCGAtcact                                   >  2:3524494/1‑89 (MQ=255)

                                                       cATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGttt                                  <  2:8638932/93‑1 (MQ=255)

                                                         tctcTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTAc                                <  2:4372642/90‑1 (MQ=255)

                                                         tCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTAc                                <  1:4781270/93‑1 (MQ=255)

                                                          cACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACt                               >  2:5037392/1‑93 (MQ=255)

                                                            cTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTgg                             >  1:61024/1‑93 (MQ=255)

                                                             tGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGc                            <  2:8814878/93‑1 (MQ=255)

                                                              gtagtaGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCg                           <  1:4427465/93‑1 (MQ=255)

                                                               tagtagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACCAAGCGATGGTTTACTGGCGa                          >  2:872589/1‑93 (MQ=255)

                                                               tagtagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGa                          >  1:5180174/1‑93 (MQ=255)

                                                                agtagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGaa                         <  1:5740368/93‑1 (MQ=255)

                                                                  tagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAgg                       >  1:3855670/1‑93 (MQ=255)

                                                                   agGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAggg                      >  1:6650392/1‑92 (MQ=255)

                                                                     gTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTgg                    >  2:2744242/1‑93 (MQ=255)

                                                                        aTGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGaa                 <  1:8670102/93‑1 (MQ=255)

                                                                        aTGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGaa                 <  2:1696403/93‑1 (MQ=255)

                                                                        aTGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGaa                 <  1:2174536/93‑1 (MQ=255)

                                                                         tGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAAt                <  2:3812524/93‑1 (MQ=255)

                                                                           cGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATgg              >  1:1012965/1‑93 (MQ=255)

                                                                           cGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATgg              <  1:6871780/93‑1 (MQ=255)

                                                                            gACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATggg             <  1:30575/93‑1 (MQ=255)

                                                                            gACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATggg             >  2:5942729/1‑93 (MQ=255)

                                                                             acacCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGc            >  1:2660827/1‑93 (MQ=255)

                                                                             acacCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGc            >  2:2684293/1‑93 (MQ=255)

                                                                             acacCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGc            >  1:8281449/1‑93 (MQ=255)

                                                                              caccacAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCa           <  2:3281742/93‑1 (MQ=255)

                                                                               accacAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCAt          >  2:6801928/1‑93 (MQ=255)

                                                                                  acaACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATggg       <  1:3644553/93‑1 (MQ=255)

                                                                                  acaACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTAGCGAAGGTGGGAATGGGCATggg       >  2:4732084/1‑93 (MQ=255)

                                                                                   caACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATgggg      <  1:2775287/93‑1 (MQ=255)

                                                                                     aCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTc    >  1:1837251/1‑93 (MQ=255)

                                                                                      cccTCTTCAGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTcc   >  2:1112028/1‑93 (MQ=255)

CGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTTTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCA  >  NC_000913/2824707‑2824886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`