BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,929,074	A→G	intergenic (+109/‑3)	sdaB → / → b2798	L‑serine dehydratase/Exo

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,929,074	0	A	G	99.7%	1135.7 / ‑5.9	338	intergenic (+109/‑3)	sdaB/b2798	L‑serine dehydratase/Exo
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (169/168); total (169/169)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 357 total aligned reads displayed.

GTTTTGCGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAACCTGC  >  NC_000913/2928984‑2929163

   ttGCGAGCCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTg                                                                                      <  2:5964509/93‑1 (MQ=255)

   tggCGGGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTg                                                                                      <  1:3651197/91‑1 (MQ=255)

    tGCGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTgg                                                                                     >  1:297311/1‑93 (MQ=255)

     gCGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGc                                                                                    <  2:905680/93‑1 (MQ=255)

     gCGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGc                                                                                    >  1:5948694/1‑93 (MQ=255)

     gCGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGc                                                                                    >  2:396044/1‑93 (MQ=255)

      cGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCt                                                                                   <  1:8672195/93‑1 (MQ=255)

       gAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTTGCGTGGCTg                                                                                  >  1:3220400/1‑93 (MQ=255)

       gAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTg                                                                                  >  2:7769485/1‑93 (MQ=255)

        aGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGt                                                                                 <  2:2549084/93‑1 (MQ=255)

        aGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGt                                                                                 >  1:7388970/1‑93 (MQ=255)

        aGGCCTCTTCCCGATTTCTCATCCAGCCGCAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGt                                                                                 >  1:6771490/1‑93 (MQ=255)

          gCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGttt                                                                               >  1:6472278/1‑92 (MQ=255)

           ccTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCa                                                                              <  2:2474113/93‑1 (MQ=255)

            ctctTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCAt                                                                             <  2:8381631/93‑1 (MQ=255)

             tctTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCAtt                                                                            >  1:7235421/1‑93 (MQ=255)

              ctTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCAttt                                                                           <  1:162370/93‑1 (MQ=255)

               ttCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTg                                                                          >  2:2256576/1‑93 (MQ=255)

               ttCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTg                                                                          >  2:3821396/1‑93 (MQ=255)

                   cGATTTCTCATCCAGCCGTATCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTa                                                                      <  1:7666788/93‑1 (MQ=255)

                   cGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTa                                                                      <  2:3294342/93‑1 (MQ=255)

                   cGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTa                                                                      <  1:5216517/93‑1 (MQ=255)

                   cGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGGTGTCGTGGCTGTTCATTTGCTTa                                                                      >  1:1897850/1‑93 (MQ=255)

                    gATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTAt                                                                     >  2:6770323/1‑93 (MQ=255)

                    gATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTAt                                                                     <  1:4288050/93‑1 (MQ=255)

                    gATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTAt                                                                     >  2:2111702/1‑93 (MQ=255)

                    gATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTAt                                                                     >  1:6377212/1‑93 (MQ=255)

                    gATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTAt                                                                     <  2:4107916/93‑1 (MQ=255)

                     aTTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTAtt                                                                    >  2:2688293/1‑93 (MQ=255)

                     aTTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTAtt                                                                    >  2:512656/1‑93 (MQ=255)

                      tttCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTg                                                                   <  1:1064169/93‑1 (MQ=255)

                        tctcATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTc                                                                 <  2:8080367/93‑1 (MQ=255)

                          tcATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGa                                                               <  1:1785734/93‑1 (MQ=255)

                           cATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGAt                                                              >  1:6600991/1‑93 (MQ=255)

                               cAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCAc                                                          >  1:8390028/1‑93 (MQ=255)

                               cAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCAc                                                          <  2:7517373/93‑1 (MQ=255)

                                   cGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGaa                                                      >  1:2175253/1‑93 (MQ=255)

                                    gTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCCATGCACTGAAt                                                     >  1:7562767/1‑93 (MQ=255)

                                      aGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCt                                                   >  2:1710135/1‑93 (MQ=255)

                                      aGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTACTTATTGTCGATGCACTGAATCt                                                   >  2:4746559/1‑93 (MQ=255)

                                         cTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATTTTAt                                                >  1:1315401/1‑93 (MQ=255)

                                         cTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTAt                                                >  1:3795057/1‑93 (MQ=255)

                                         cTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTAt                                                <  2:6283168/93‑1 (MQ=255)

                                         cTGTTCCCGCATCGGATGTTACCCCTATCGCCTTATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTAt                                                <  1:8469650/93‑1 (MQ=255)

                                          tGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTAtt                                               >  2:586750/1‑93 (MQ=255)

                                          tGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTAtt                                               >  1:7090151/1‑93 (MQ=255)

                                           gTTCCGGCAGCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATtc                                              <  2:6648736/93‑1 (MQ=255)

                                            ttCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATtcg                                             <  1:3425890/93‑1 (MQ=255)

                                            ttCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATacg                                             <  1:5517936/93‑1 (MQ=255)

                                              ccGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATtcgtc                                           <  1:8630761/93‑1 (MQ=255)

                                                  gaTCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCAt                                       <  2:7195131/92‑1 (MQ=255)

                                                  cATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCAt                                       <  1:2804682/93‑1 (MQ=255)

                                                  cATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTAATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCAt                                       <  2:5278118/93‑1 (MQ=255)

                                                    tCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTc                                     >  2:1594336/1‑93 (MQ=255)

                                                     cGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCa                                    <  2:8252707/93‑1 (MQ=255)

                                                      gAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCAt                                   >  1:964714/1‑93 (MQ=255)

                                                      gAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCAt                                   >  2:1536072/1‑93 (MQ=255)

                                                      gAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCAt                                   >  2:1353164/1‑93 (MQ=255)

                                                       aaTGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATg                                  >  2:4413596/1‑93 (MQ=255)

                                                       aaTGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATg                                  <  2:7273391/93‑1 (MQ=255)

                                                        aTGTTACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGAGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGc                                 >  1:3297456/1‑93 (MQ=255)

                                                           ttACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGt                              <  2:6888496/93‑1 (MQ=255)

                                                            tACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGtt                             >  1:3553683/1‑93 (MQ=255)

                                                            tACCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGtt                             >  1:2640970/1‑93 (MQ=255)

                                                             aCCCTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTc                            >  2:3643328/1‑93 (MQ=255)

                                                              cccTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTcg                           >  1:2414127/1‑92 (MQ=255)

                                                              cccTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCa                           <  2:3571279/93‑1 (MQ=255)

                                                              cccTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCa                           >  1:6548412/1‑93 (MQ=255)

                                                                cTTATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAgg                         <  2:2790502/93‑1 (MQ=255)

                                                                 ttATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCTCTGAATCTTATTCGTCGCATTCATGCCGTTCAggg                        <  2:1183639/93‑1 (MQ=255)

                                                                 ttATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAggg                        >  1:414717/1‑93 (MQ=255)

                                                                  tATCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAgggg                       <  2:4618857/93‑1 (MQ=255)

                                                                   aTCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGt                      <  2:4304507/93‑1 (MQ=255)

                                                                   aTCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGt                      <  1:555168/93‑1 (MQ=255)

                                                                   aTCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGt                      <  1:6104521/93‑1 (MQ=255)

                                                                   aTCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGt                      <  2:3187390/93‑1 (MQ=255)

                                                                    tCGCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTc                     <  1:4605686/93‑1 (MQ=255)

                                                                      gCCTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCCGGGGTCGc                   >  2:4541542/1‑93 (MQ=255)

                                                                        cTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGccc                 >  1:6911663/1‑93 (MQ=255)

                                                                        cTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGccc                 >  2:3061924/1‑93 (MQ=255)

                                                                        cTGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGccc                 <  1:7956287/93‑1 (MQ=255)

                                                                         tGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCt                <  1:602183/93‑1 (MQ=255)

                                                                         tGATCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCt                <  1:2374517/93‑1 (MQ=255)

                                                                         tGATCTTTAAGGGGGTTGTCGTGGCTGGTCATTTGCTTATTGTCGATGCACTGAATGTTATTTTTCGCATTCATGCCGTTCAGGTGTCGCCCt                >  2:7379778/1‑93 (MQ=255)

                                                                          gATCTTTAAGGGGGTTGTCGCGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCtg               >  2:1801694/1‑93 (MQ=255)

                                                                            tCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCtgtg             <  1:4955398/93‑1 (MQ=255)

                                                                            tCTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCtgtg             >  2:199591/1‑93 (MQ=255)

                                                                             cTTTAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTgtgt            <  1:8317673/93‑1 (MQ=255)

                                                                              tttAAGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTc           >  1:2879958/1‑93 (MQ=255)

                                                                                 aaGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCgca        >  1:114639/1‑91 (MQ=255)

                                                                                  aGGGGGTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGaaa       >  2:3715246/1‑93 (MQ=255)

                                                                                   gggggTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAAc      >  1:3969098/1‑93 (MQ=255)

                                                                                   gggggTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAAc      <  2:8519660/93‑1 (MQ=255)

                                                                                    ggggTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAAcc     >  1:4411249/1‑93 (MQ=255)

                                                                                    ggggTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAAcc     >  2:5413060/1‑93 (MQ=255)

                                                                                    ggggTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAAcc     <  2:487299/93‑1 (MQ=255)

                                                                                      ggTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAACCTg   >  2:6432321/1‑93 (MQ=255)

                                                                                      ggTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAACCTg   >  2:5673129/1‑93 (MQ=255)

                                                                                       gTTGTCGTGGCTGTTCATTTGCTTATTGTGAATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAACCTGc  <  1:7850762/93‑1 (MQ=255)

                                                                                       gTTGTCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAACCTGc  <  1:4677054/93‑1 (MQ=255)

GTTTTGCGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTACCCTTATCGCCTGATCTTTAAGGGGGTTATCGTGGCTGTTCATTTGCTTATTGTCGATGCACTGAATCTTATTCGTCGCATTCATGCCGTTCAGGGGTCGCCCTGTGTCGAAACCTGC  >  NC_000913/2928984‑2929163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`