BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,158,281	C→T	L294L (CTG→CTA)	ygiRQ ←	Fe‑S oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,158,281	0	C	T	99.5%	1340.5 / NA	383	L294L (CTG→CTA)	ygiRQ	Fe‑S oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (0/2); major base T (160/219); minor base G (1/1); total (161/222)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 397 total aligned reads displayed.

GCGGGCACAG‑CTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGTTT  >  NC_000913/3158192‑3158370

  gggCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGgtagca                                                                                       <  1:700214/93‑1 (MQ=255)

   ggCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCaa                                                                                      >  2:6550443/1‑93 (MQ=255)

     cacaGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAaca                                                                                    >  2:596810/1‑93 (MQ=255)

     cacaGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAaca                                                                                    <  1:6854108/93‑1 (MQ=255)

       cagccTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACg                                                                                  <  1:7227245/89‑1 (MQ=255)

        agccTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGt                                                                                 >  1:5341155/4‑93 (MQ=255)

         gccTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTa                                                                                >  1:4617316/3‑93 (MQ=255)

         g‑CTGGGTTTGTTGCGGGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAg                                                                               <  1:6273294/93‑1 (MQ=255)

          ccTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAg                                                                               <  1:2451914/92‑1 (MQ=255)

          ccTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAg                                                                               >  2:3305227/2‑93 (MQ=255)

           cTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAATAAGGTAGCAACACGTAgg                                                                              >  2:8521244/1‑93 (MQ=255)

            tGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGt                                                                             <  2:1651257/93‑1 (MQ=255)

            tGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGt                                                                             >  1:6544563/1‑93 (MQ=255)

            tGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGt                                                                             <  2:6783947/93‑1 (MQ=255)

            tGGGTTGGTTTCGTAGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGt                                                                             <  1:4541/93‑1 (MQ=255)

             gggTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGtt                                                                            <  2:6144450/93‑1 (MQ=255)

             gggTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGtt                                                                            <  2:454903/93‑1 (MQ=255)

              tgtttgtttCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGttt                                                                           <  2:8703693/92‑1 (MQ=255)

               gttggttTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGtttt                                                                          >  2:2607786/1‑93 (MQ=255)

                 tggttTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGtttttt                                                                        >  2:1257729/1‑93 (MQ=255)

                   gttTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTcc                                                                      >  1:2826189/1‑93 (MQ=255)

                   gttTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTcc                                                                      <  2:7666434/93‑1 (MQ=255)

                      tCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCAc                                                                   <  2:172468/93‑1 (MQ=255)

                        gtggtgCAGAATACGCGAAGAATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACgg                                                                 >  1:6174139/1‑93 (MQ=255)

                         tggtgCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGt                                                                >  1:4047061/1‑93 (MQ=255)

                         tggtgCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGt                                                                <  1:7272784/93‑1 (MQ=255)

                         tggtgCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGt                                                                >  1:6422252/1‑93 (MQ=255)

                          ggtgCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGtt                                                               >  1:7730681/1‑93 (MQ=255)

                          ggtgCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGtt                                                               <  1:2716897/93‑1 (MQ=255)

                           gtgCAGAATACGCGAAGCATGTGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGttt                                                              >  1:1522193/1‑93 (MQ=255)

                           gtgCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGttt                                                              <  2:1992243/93‑1 (MQ=255)

                           gtgCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGttt                                                              <  1:7826303/93‑1 (MQ=255)

                           gtgCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGttt                                                              <  2:2920420/93‑1 (MQ=255)

                            tgCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTc                                                             <  1:5166985/93‑1 (MQ=255)

                            tgCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTc                                                             <  2:3140588/93‑1 (MQ=255)

                            tgCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAACAACACGTAGGTTTTTTCCCACGGTTTc                                                             <  2:6625030/93‑1 (MQ=255)

                              cAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCgg                                                           <  1:2212785/93‑1 (MQ=255)

                              cAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCgg                                                           >  2:8401565/1‑93 (MQ=255)

                              cAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCgg                                                           <  1:7471638/93‑1 (MQ=255)

                              cAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCgg                                                           <  1:1678359/93‑1 (MQ=255)

                              cAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCgg                                                           <  1:8684814/93‑1 (MQ=255)

                                gANTACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTANCAACACGTAGGTTTTTTNCCNNGGTTTCGGgc                                                         >  2:3989563/1‑93 (MQ=255)

                                gAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGgc                                                         <  1:2023483/93‑1 (MQ=255)

                                gAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGgc                                                         >  1:5456452/1‑93 (MQ=255)

                                gAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGgc                                                         <  2:712054/93‑1 (MQ=255)

                                   tACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGcgcg                                                      <  1:7393708/93‑1 (MQ=255)

                                    aCGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCgg                                                     >  2:384821/1‑93 (MQ=255)

                                    aCGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCgg                                                     <  2:6921206/93‑1 (MQ=255)

                                     cgcgAAGGATGGGCGTACAGCTCTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGt                                                    <  2:2178130/93‑1 (MQ=255)

                                     cgcgAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGt                                                    <  2:7937939/93‑1 (MQ=255)

                                       cgAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTgg                                                  <  2:2477563/93‑1 (MQ=255)

                                        gAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGc                                                 <  2:7552829/93‑1 (MQ=255)

                                        gAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGc                                                 <  2:5409869/93‑1 (MQ=255)

                                          aGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTg                                               <  2:913917/93‑1 (MQ=255)

                                           gCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGc                                              >  2:1021053/1‑93 (MQ=255)

                                            cATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCa                                             <  1:3749843/93‑1 (MQ=255)

                                               gggCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACgg                                          <  2:5663379/93‑1 (MQ=255)

                                                ggCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGt                                         <  1:8085361/93‑1 (MQ=255)

                                                  cGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTa                                       <  2:3572634/93‑1 (MQ=255)

                                                  cGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTa                                       <  2:2728489/93‑1 (MQ=255)

                                                   gTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTAc                                      >  1:3907236/1‑93 (MQ=255)

                                                    tACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACg                                     >  1:1925655/1‑93 (MQ=255)

                                                      cAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGGTTCGAGCGCGGTGGCTGCACGGTTACGGc                                   >  2:5193187/1‑93 (MQ=255)

                                                       aGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCt                                  <  1:1053502/93‑1 (MQ=255)

                                                        gCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCtt                                 <  1:117021/93‑1 (MQ=255)

                                                        gCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCtt                                 >  1:3499109/1‑93 (MQ=255)

                                                         cACTTTATCTCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCttt                                <  2:1395792/93‑1 (MQ=255)

                                                         cACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCttt                                <  1:6746026/93‑1 (MQ=255)

                                                            tttATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGc                             >  1:1347178/1‑93 (MQ=255)

                                                               aTCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACAGTTACGGCTTTGGCTTc                          >  2:6507763/1‑93 (MQ=255)

                                                                 cGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCt                        <  1:2373664/93‑1 (MQ=255)

                                                                 cGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCt                        <  1:8314797/93‑1 (MQ=255)

                                                                 cGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGGTTTTTCCCACGGTTTTGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCt                        >  2:4495082/1‑93 (MQ=255)

                                                                  gCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTg                       >  1:3323511/1‑93 (MQ=255)

                                                                   cccTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGc                      <  2:8280783/93‑1 (MQ=255)

                                                                    ccTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCt                     >  2:4924183/1‑93 (MQ=255)

                                                                    ccTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCt                     <  1:476645/93‑1 (MQ=255)

                                                                     cTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCAAACGATTTCGGGCGCGGTGGCTGCACGGTTACGACTTTGACTTCCTACtt                    <  2:7845810/93‑1 (MQ=255)

                                                                      ttCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCttt                   >  2:3043985/1‑93 (MQ=255)

                                                                      ttCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCttt                   <  1:952674/93‑1 (MQ=255)

                                                                      ttCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCttt                   <  1:8480244/93‑1 (MQ=255)

                                                                      ttCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCttt                   >  1:5693307/1‑93 (MQ=255)

                                                                       tCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCtttt                  >  1:5123372/1‑93 (MQ=255)

                                                                       tCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCtttt                  >  1:3112501/1‑93 (MQ=255)

                                                                        cACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCttttt                 >  1:3591085/1‑93 (MQ=255)

                                                                        cACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCttttt                 >  2:2317677/1‑93 (MQ=255)

                                                                         aCTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCtttttg                >  2:4275703/1‑92 (MQ=255)

                                                                         aCTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTc                <  2:5854187/93‑1 (MQ=255)

                                                                           tttCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCgg              >  1:4318332/1‑93 (MQ=255)

                                                                           tttCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCgg              >  1:7647235/1‑93 (MQ=255)

                                                                            ttCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGt             <  2:1613502/93‑1 (MQ=255)

                                                                            ttCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGt             >  1:4442950/1‑93 (MQ=255)

                                                                             tctcGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTg            >  1:5791203/1‑93 (MQ=255)

                                                                              ctcGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGc           >  1:5608016/1‑93 (MQ=255)

                                                                               tcGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCGCGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGcc          >  2:7296289/1‑93 (MQ=255)

                                                                               tcGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGcc          >  2:2099796/1‑93 (MQ=255)

                                                                                     gaagGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGt    >  1:2981127/1‑93 (MQ=255)

                                                                                      aagGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGtt   <  1:1884745/93‑1 (MQ=255)

                                                                                      aagGTAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGtt   >  2:4382487/1‑93 (MQ=255)

                                                                                      aagGTAGCAACACGTAGGTTTTTACCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGtt   <  2:4737698/93‑1 (MQ=255)

GCGGGCACAG‑CTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGTTT  >  NC_000913/3158192‑3158370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`