BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,195,844	A→G	V480A (GTG→GCG)	glnE ←	GlnE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,195,844	0	A	G	99.7%	1130.0 / NA	331	V480A (GTG→GCG)	glnE	GlnE
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (188/142); total (188/143)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 338 total aligned reads displayed.

TGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCACGCCACTGT  >  NC_000913/3195754‑3195933

tGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGcc                                                                                         <  2:7426448/93‑1 (MQ=255)

  cGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTg                                                                                       >  1:1542666/1‑93 (MQ=255)

    gCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGtt                                                                                     <  1:1409268/93‑1 (MQ=255)

     cATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGttt                                                                                    <  2:19541/93‑1 (MQ=255)

     cATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGttt                                                                                    >  1:2647528/1‑93 (MQ=255)

      aTCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTg                                                                                   <  1:1103849/93‑1 (MQ=255)

      aTCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTg                                                                                   <  1:89107/93‑1 (MQ=255)

      aTCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTg                                                                                   >  1:4371743/1‑93 (MQ=255)

      aTCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTg                                                                                   >  2:3039220/1‑93 (MQ=255)

       tCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGc                                                                                  >  2:5659869/1‑93 (MQ=255)

         aGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGa                                                                                <  2:6475475/93‑1 (MQ=255)

         aGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGa                                                                                >  1:1810730/1‑93 (MQ=255)

         aGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGa                                                                                <  1:5484317/93‑1 (MQ=255)

          gATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGat                                                                               >  2:2819277/1‑93 (MQ=255)

          gATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGat                                                                               >  2:2421230/1‑93 (MQ=255)

            tGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGatca                                                                             >  1:2486084/1‑93 (MQ=255)

             ggTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGatcat                                                                            <  1:3276912/93‑1 (MQ=255)

             ggTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGatcat                                                                            <  1:7286545/93‑1 (MQ=255)

              gTCGAGCACCTGACGTCCTCGCGGCCCGATCGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGatcatc                                                                           >  2:3657631/1‑93 (MQ=255)

               tCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATcc                                                                          <  1:5118748/93‑1 (MQ=255)

               tCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATcc                                                                          >  2:511305/1‑93 (MQ=255)

                cGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCt                                                                         >  1:6096875/1‑93 (MQ=255)

                cGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCt                                                                         <  1:4087493/93‑1 (MQ=255)

                  aGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCg                                                                       <  1:5010402/93‑1 (MQ=255)

                   gCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGc                                                                      <  2:4688980/93‑1 (MQ=255)

                      ccTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTaa                                                                   <  2:8120717/93‑1 (MQ=255)

                      ccTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTaa                                                                   >  2:1387437/1‑93 (MQ=255)

                        tGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGa                                                                 <  1:4919020/93‑1 (MQ=255)

                         gACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGAt                                                                >  1:2940444/1‑93 (MQ=255)

                           cGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATgc                                                              >  1:4585978/1‑93 (MQ=255)

                            gTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATgcg                                                             >  1:6535827/1‑93 (MQ=255)

                            gTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATgcg                                                             >  2:1461134/1‑93 (MQ=255)

                            gTCCTCGCGGCCCGATGGTGCGATTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATgcg                                                             <  2:5936393/93‑1 (MQ=255)

                             tCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATgcgc                                                            <  1:6695716/93‑1 (MQ=255)

                             tCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATgcgc                                                            >  2:4171411/1‑93 (MQ=255)

                                 cgcgGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGc                                                        >  2:2429999/1‑93 (MQ=255)

                                  gcgGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCa                                                       >  1:8311541/1‑93 (MQ=255)

                                   cgGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCAc                                                      <  1:4262947/93‑1 (MQ=255)

                                    ggCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGACAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACt                                                     >  2:5401416/1‑93 (MQ=255)

                                     gCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTg                                                    >  2:87979/1‑93 (MQ=255)

                                      cccGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTgg                                                   <  1:2072014/93‑1 (MQ=255)

                                      cccGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTgg                                                   <  2:2603256/93‑1 (MQ=255)

                                      cccGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTgg                                                   <  2:6668602/93‑1 (MQ=255)

                                          aTGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTa                                               >  1:3444513/1‑93 (MQ=255)

                                          aTGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTa                                               >  2:1830917/1‑93 (MQ=255)

                                            ggTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAgt                                             >  1:3161267/1‑93 (MQ=255)

                                             gTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAgtg                                            <  1:6839591/93‑1 (MQ=255)

                                               gcgcTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTc                                          <  1:3732892/93‑1 (MQ=255)

                                                cgcTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCa                                         <  1:7079975/93‑1 (MQ=255)

                                                  cTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATc                                       >  2:4482197/1‑93 (MQ=255)

                                                  cTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATc                                       >  1:803377/1‑93 (MQ=255)

                                                   ttATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCt                                      >  1:1989099/1‑93 (MQ=255)

                                                   ttATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCt                                      >  2:5990921/1‑93 (MQ=255)

                                                     aTCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTc                                    >  1:972993/1‑93 (MQ=255)

                                                      tCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTcc                                   >  2:425774/1‑93 (MQ=255)

                                                      tCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTcc                                   <  1:5780594/93‑1 (MQ=255)

                                                      tCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTcc                                   >  1:6002853/1‑93 (MQ=255)

                                                       ccAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCt                                  >  1:7622712/1‑93 (MQ=255)

                                                        cAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTg                                 <  1:523368/93‑1 (MQ=255)

                                                           ctctTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCaa                              <  1:6339397/93‑1 (MQ=255)

                                                            tctTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAAc                             <  2:7612781/93‑1 (MQ=255)

                                                            tctTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAAc                             >  1:7955812/1‑93 (MQ=255)

                                                            tctTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAAc                             >  2:3210960/1‑93 (MQ=255)

                                                             ctTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACg                            <  1:7198291/93‑1 (MQ=255)

                                                              tttGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGc                           <  2:1240520/93‑1 (MQ=255)

                                                               ttGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCa                          <  2:625015/93‑1 (MQ=255)

                                                               ttGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCa                          <  1:219542/93‑1 (MQ=255)

                                                               ttGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCa                          <  2:5148393/93‑1 (MQ=255)

                                                                  cGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATcc                       >  2:4846512/1‑93 (MQ=255)

                                                                  cGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATcc                       <  1:5906770/93‑1 (MQ=255)

                                                                   ggAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCt                      >  2:1360992/1‑93 (MQ=255)

                                                                    gAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTg                     >  1:4499843/1‑93 (MQ=255)

                                                                    gAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTg                     >  2:4019360/1‑93 (MQ=255)

                                                                    gAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTg                     <  2:7033234/93‑1 (MQ=255)

                                                                    gAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTg                     >  1:3837526/1‑93 (MQ=255)

                                                                      aaTCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGcc                   <  2:5547851/93‑1 (MQ=255)

                                                                      aaTCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGcc                   >  1:1004066/1‑93 (MQ=255)

                                                                       aTCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCca                  <  1:3007705/93‑1 (MQ=255)

                                                                       aTCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCca                  >  1:8712643/1‑93 (MQ=255)

                                                                        tCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCcac                 >  2:6896876/1‑93 (MQ=255)

                                                                         cGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCcaca                <  1:8112713/93‑1 (MQ=255)

                                                                          ggCAATCAGCGTTAGCAACTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAg               <  1:7824242/93‑1 (MQ=255)

                                                                           gCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGc              <  2:214647/93‑1 (MQ=255)

                                                                            cAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCt             <  1:1742780/93‑1 (MQ=255)

                                                                             aaTCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTc            >  2:8520667/1‑93 (MQ=255)

                                                                              aTCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCa           <  1:1262656/93‑1 (MQ=255)

                                                                               tCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCAc          >  1:3516027/1‑93 (MQ=255)

                                                                               tCAGCGTTAGCGCCTGTTTGCAATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCAc          <  1:6901628/93‑1 (MQ=255)

                                                                                 aGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCACGc        >  1:5605420/1‑93 (MQ=255)

                                                                                 aGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCACGc        <  1:2848688/93‑1 (MQ=255)

                                                                                 aGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCACGc        >  2:7760658/1‑93 (MQ=255)

                                                                                 aGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGACAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCACGc        >  1:1599729/1‑93 (MQ=255)

                                                                                  gCGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCACGcc       >  2:155022/1‑93 (MQ=255)

                                                                                   cGTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCACGCCa      >  1:3980014/1‑93 (MQ=255)

                                                                                    gTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCACGCCAc     >  2:7297195/1‑93 (MQ=255)

                                                                                    gTTAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCACGCCAc     <  1:6430011/93‑1 (MQ=255)

                                                                                     ttAGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCACGCCACt    >  1:3572149/1‑93 (MQ=255)

                                                                                      tAGCGCCTGTTTGCAATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCACCGCATCCTGCCACAGCTCACGCCACTg   <  2:7990050/93‑1 (MQ=255)

                                                                                       aGCGCCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCACGCCACTGt  >  2:3598013/1‑93 (MQ=255)

                                                                                       aGCGCCTGTTTGCGAACATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCACGCCACTGt  >  1:8588152/1‑93 (MQ=255)

TGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAAGATGCGCCAGCACTGGCGTAGTGTCATCTTCCTGCAACGCATCCTGCCACAGCTCACGCCACTGT  >  NC_000913/3195754‑3195933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`