Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,446,406 G→A T134M (ACG→ATG)  rplP ← 50S ribosomal subunit protein L16

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,446,4060GA99.5% 1418.1 / ‑5.6 400T134M (ACG→ATG) rplP50S ribosomal subunit protein L16
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (172/226);  total (173/227)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.20e-01

Only 100 of 409 total aligned reads displayed.

GTTGAACTGCTCACGCAGCAGGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCGTCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACGGGCCAGCTCTTCCGGAACACCGTCCATTTCA  >  NC_000913/3446316‑3446498
                                                                                          |                                                                                            
 ttGAACTGCTCACGCAGCAGGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCt                                                                                           <  1:323764/93‑1 (MQ=255)
 gtGAACTGCTCACGCAGCAGGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCt                                                                                           <  2:7628684/92‑1 (MQ=255)
  tGAACTGCTCACGCAGCAGGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCtt                                                                                          >  2:3451500/1‑93 (MQ=255)
  tGAACTGCTCACGCAGCAGGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCtt                                                                                          <  1:8279101/93‑1 (MQ=255)
   gAACTGCTCACGCAGCAGGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTa                                                                                         <  1:1040890/93‑1 (MQ=255)
    aaCTGCTCACGCAGCAGGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAg                                                                                        <  2:5107965/93‑1 (MQ=255)
     aCTGCTCACGCAGCAGGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGt                                                                                       <  1:4120666/93‑1 (MQ=255)
     aCTGCTCACGCAGCAGGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGt                                                                                       <  1:3653347/93‑1 (MQ=255)
       tGCTCACGCAGCAGGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTa                                                                                     <  1:1377153/93‑1 (MQ=255)
        gCTCACGCAGCAGGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTAc                                                                                    <  1:6008019/93‑1 (MQ=255)
        gCTCACGCAGCAGGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTAc                                                                                    <  2:4743978/93‑1 (MQ=255)
        gCTCACGCAGCAGGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTAc                                                                                    <  2:222283/93‑1 (MQ=255)
        gCTCACGCAGCAGGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTAc                                                                                    >  1:4812274/1‑93 (MQ=255)
          tCACGCAGCAGGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACaa                                                                                  >  1:6432567/1‑93 (MQ=255)
           cACGCAGCAGGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACaaa                                                                                 <  1:3952316/93‑1 (MQ=255)
             cGCAGCAGGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAgg                                                                               >  1:7378669/1‑93 (MQ=255)
             cGCAGCAGGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAgg                                                                               <  1:3710096/93‑1 (MQ=255)
              gcagcaGGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAggt                                                                              <  1:3517983/93‑1 (MQ=255)
              gcagcaGGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAggt                                                                              <  2:2829498/93‑1 (MQ=255)
              gcagcaGGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAggt                                                                              <  1:7954560/93‑1 (MQ=255)
              gcagcaGGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAggt                                                                              >  2:7959096/1‑93 (MQ=255)
              gcagcaGGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAggt                                                                              >  2:4585295/1‑93 (MQ=255)
              gcagcaGGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAggg                                                                              >  2:2569126/1‑92 (MQ=255)
               cagcagGTTAAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTAGCTTTCATTACATCACCATCTTAGTTACAAAggtg                                                                             <  2:4406841/93‑1 (MQ=255)
                agcagGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAggtgg                                                                            <  1:5239419/93‑1 (MQ=255)
                agcagGGTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAggtgg                                                                            >  2:8019773/1‑93 (MQ=255)
                 gcagGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAggtggt                                                                           >  1:4525129/1‑93 (MQ=255)
                 gcagGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAggtggt                                                                           >  2:1896658/1‑93 (MQ=255)
                 gcagGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAggtggt                                                                           <  2:7433869/93‑1 (MQ=255)
                  cagGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGtt                                                                          <  2:1373175/93‑1 (MQ=255)
                  cagGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGtt                                                                          >  1:498229/1‑93 (MQ=255)
                   agGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGttt                                                                         >  1:6300499/1‑93 (MQ=255)
                    ggTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGtttt                                                                        <  2:6627182/93‑1 (MQ=255)
                    ggTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGtttt                                                                        >  2:1969721/1‑93 (MQ=255)
                     gTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTa                                                                       >  1:12099/1‑93 (MQ=255)
                      ttCAGCATCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTaa                                                                      <  2:895182/93‑1 (MQ=255)
                      ttCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTaa                                                                      <  2:31508/93‑1 (MQ=255)
                      ttCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTaa                                                                      <  1:8799300/93‑1 (MQ=255)
                       tCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAAt                                                                     <  1:3404800/93‑1 (MQ=255)
                        tagcagcTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATc                                                                    <  1:3003156/92‑1 (MQ=255)
                        cagcagCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATc                                                                    <  2:359224/93‑1 (MQ=255)
                        cagcagCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATc                                                                    <  1:2232335/93‑1 (MQ=255)
                         agcagcTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCg                                                                   <  2:3814177/93‑1 (MQ=255)
                         agcagcTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCg                                                                   <  2:6913081/93‑1 (MQ=255)
                         agcagcTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCg                                                                   >  1:2132705/1‑93 (MQ=255)
                          gcagcTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATGACCATCTTAGTTACAAAGGTGGTTTTAATCgc                                                                  >  2:1625177/1‑92 (MQ=255)
                               tCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGtt                                                             >  1:613427/1‑93 (MQ=255)
                                cGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCGTCTTAGTTACAAAGGTGGTTTTAATCGGCAGttt                                                            <  2:5893187/93‑1 (MQ=255)
                                cGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGttt                                                            <  2:1170166/93‑1 (MQ=255)
                                  gtgtTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGtttgc                                                          >  2:7574988/1‑91 (MQ=255)
                                    gtTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCgct                                                        <  2:4185301/93‑1 (MQ=255)
                                     ttCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCgctg                                                       >  1:623338/1‑93 (MQ=255)
                                      tCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCgctgc                                                      <  2:2373723/93‑1 (MQ=255)
                                       cAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCgctgct                                                     <  1:851001/93‑1 (MQ=255)
                                        aGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGctgctg                                                    >  2:8633893/1‑93 (MQ=255)
                                           tctTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCa                                                 <  2:8297097/93‑1 (MQ=255)
                                           tctTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCa                                                 >  1:8159044/1‑93 (MQ=255)
                                           tctTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCa                                                 >  2:5790975/1‑93 (MQ=255)
                                            ctTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAg                                                >  2:5559247/1‑93 (MQ=255)
                                             ttCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGc                                               >  1:6540115/1‑93 (MQ=255)
                                               cAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCtt                                             <  2:3721066/93‑1 (MQ=255)
                                                 aCGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACACAGGTGGTTTGAATCGGCAGTTTCGCTGCTGCCAGCTTGa                                           >  1:6672382/1‑93 (MQ=255)
                                                   gCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAAt                                         <  2:5956019/93‑1 (MQ=255)
                                                     tcttctCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGc                                       <  1:5806747/93‑1 (MQ=255)
                                                      cttctCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCt                                      <  2:1017419/93‑1 (MQ=255)
                                                       ttctCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCtt                                     >  2:2454612/1‑93 (MQ=255)
                                                       ttctCACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCtt                                     <  2:7013594/93‑1 (MQ=255)
                                                        tctcACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTc                                    >  2:6431346/1‑93 (MQ=255)
                                                        tctcACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTc                                    >  2:391763/1‑93 (MQ=255)
                                                           cACGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACg                                 >  2:6255643/1‑93 (MQ=255)
                                                           cACGCAGCTCTTTTGCTTTCATT‑CATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACgg                                <  2:4868786/93‑1 (MQ=255)
                                                            aCGCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACgg                                >  1:1932098/1‑93 (MQ=255)
                                                              gCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACGGGc                              >  2:1235648/1‑93 (MQ=255)
                                                              gCAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACGGGc                              <  2:5511324/93‑1 (MQ=255)
                                                               cAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACGGGcc                             <  2:113509/93‑1 (MQ=255)
                                                               cAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACGGGcc                             <  1:7544986/93‑1 (MQ=255)
                                                               cAGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACGGGcc                             >  1:7201522/1‑93 (MQ=255)
                                                                aGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACGGGCCa                            >  1:6053948/1‑93 (MQ=255)
                                                                aGCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACGGGCCa                            >  1:6757575/1‑93 (MQ=255)
                                                                 gCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACGGGCCAg                           >  1:6975134/1‑93 (MQ=255)
                                                                 gCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACGGGCCAg                           >  2:5703853/1‑93 (MQ=255)
                                                                 gCTCTTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACGGGCCAg                           >  2:1723353/1‑93 (MQ=255)
                                                                   tctTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACGGGCCAGct                         <  1:1120353/93‑1 (MQ=255)
                                                                   tctTTTGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACGGGCCAGct                         >  2:8138461/1‑93 (MQ=255)
                                                                     ttttGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTCATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACGGGCCAGctct                       >  1:5493813/1‑93 (MQ=255)
                                                                      tttGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACGGGCCAGCTCtt                      <  1:2730033/93‑1 (MQ=255)
                                                                      tttGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACGGGCCAGCTCtt                      <  2:7264107/93‑1 (MQ=255)
                                                                       ttGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACGGGCCAGCTCTTc                     <  1:2578971/93‑1 (MQ=255)
                                                                       ttGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACGGGCCAGCTCTTc                     <  2:5175073/93‑1 (MQ=255)
                                                                        tGCTTTCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACGGGCCAGCTCTTcc                    <  2:5284847/93‑1 (MQ=255)
                                                                           tttCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACGGGCCAGCTCTTCCGGa                 >  2:8399689/1‑93 (MQ=255)
                                                                           tttCATTACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACGGGCCAGCTCTTCCGGa                 >  1:5558256/1‑93 (MQ=255)
                                                                             tCATTACATCACCATCTTAGTTACAAAGGTGGTTTTCATCGGCAGTTTCGCTGCTACCAGCTTGAATGCTTCCCGGCCCAGCTCTTCCGGAac               <  1:7157140/93‑1 (MQ=255)
                                                                                 tACATCACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACGGGCCAGCTCTTCCGGAACACCg           >  2:404792/1‑93 (MQ=255)
                                                                                  aCATCACCATATTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACGGGCCAGCTCTTCCGGAACACCGt          <  1:4472714/93‑1 (MQ=255)
                                                                                    aTCACCATCTTAGTTACAAAGGTTGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACGGGCCAGCTCTTCCGGAACACCGTcc        <  2:1569265/93‑1 (MQ=255)
                                                                                      cACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACGGGCCAGCTCTTCCGGAACACCGTCCAt      <  1:2470068/93‑1 (MQ=255)
                                                                                      cACCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACGGGCCAGCTCTTCCGGAACACCGTCCAt      <  1:8677380/93‑1 (MQ=255)
                                                                                       aCCATCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACGGGCCAGCTCTTCCGGAACACCGTCCAtt     >  1:7597795/1‑93 (MQ=255)
                                                                                          gTCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACGGGCCAGCTCTTCCGGAACACCGTCCATTTCa  >  1:1511542/1‑93 (MQ=255)
                                                                                          |                                                                                            
GTTGAACTGCTCACGCAGCAGGTTCAGCAGCTCGGTGTTCAGCTCTTCAACGCTCTTCTCACGCAGCTCTTTTGCTTTCATTACATCACCGTCTTAGTTACAAAGGTGGTTTTAATCGGCAGTTTCGCTGCTGCCAGCTTGAATGCTTCACGGGCCAGCTCTTCCGGAACACCGTCCATTTCA  >  NC_000913/3446316‑3446498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑