BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,464,165	T→C	E66E (GAA→GAG)	bfr ←	bacterioferrin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,464,165	0	T	C	99.7%	1208.7 / ‑5.2	347	E66E (GAA→GAG)	bfr	bacterioferrin
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (179/167); total (179/168)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

Only 100 of 356 total aligned reads displayed.

CATCCAGCTCAAGTGCCAGATCAGAACGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCTTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGATACTCCACATCATTGAGACGTTTGAG  >  NC_000913/3464074‑3464254

cATCCAGCTCAAGTACCAGATCAGAACGCAGCATTTACTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCtt                                                                                          <  1:4839786/93‑1 (MQ=255)

 aTCCAGCTCAAGTGCCAGATCAGAACGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTc                                                                                         >  1:4467452/1‑93 (MQ=255)

 aTCCAGCTCAAGTGCCAGATCAGAACGCAGCATTTCATCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTc                                                                                         >  1:4926226/1‑93 (MQ=255)

   ccAGCTCAAGTGCCAGATCAGAACGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCa                                                                                       <  1:8483989/93‑1 (MQ=255)

    cAGCTCAAGTGCCAGATCAGAACGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAg                                                                                      >  1:1140556/1‑93 (MQ=255)

    cAGCTCAAGTGCCAGATCAGAACGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAg                                                                                      >  2:2155026/1‑93 (MQ=255)

     aGCTCAAGTGCCAGATCAGAACGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGa                                                                                     >  2:340332/1‑93 (MQ=255)

       cTCAAGTGCCAGATCAGAACGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGaaa                                                                                   >  1:2384468/1‑93 (MQ=255)

         taaGTGCCAGATCAGAACGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGaaaaa                                                                                 <  2:6317198/92‑1 (MQ=255)

         cAAGTGCCAGATCAGAACGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGaaaaa                                                                                 <  1:6744398/93‑1 (MQ=255)

          aaGTGCCAGATCAGAACGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAg                                                                                <  1:8638763/93‑1 (MQ=255)

            gTGCCAGATCAGAACGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGaa                                                                              <  2:6395858/93‑1 (MQ=255)

             tGCCAGATCAGAACGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAAt                                                                             >  1:1045782/1‑93 (MQ=255)

                cAGATCAGAACGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATgcg                                                                          >  2:2086089/1‑93 (MQ=255)

                  gATCAGAACGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCt                                                                        >  2:8333189/1‑93 (MQ=255)

                  gATCAGAACGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCt                                                                        >  2:6752283/1‑93 (MQ=255)

                  gATCAGAACGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCt                                                                        >  1:561758/1‑93 (MQ=255)

                   aTCAGAACGCAGGATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTc                                                                       >  2:860041/1‑93 (MQ=255)

                   aTCAGAACGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTc                                                                       >  2:3782302/1‑93 (MQ=255)

                    tCAGAACGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCa                                                                      >  1:8681014/1‑93 (MQ=255)

                    tCAGAACGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCa                                                                      >  2:4236891/1‑93 (MQ=255)

                      aGAACGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAat                                                                    <  1:4331596/93‑1 (MQ=255)

                      aGAACGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAat                                                                    <  2:233315/93‑1 (MQ=255)

                       gAACGCAGCATTTCCTCAACCTCTTCACCAATGTGCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGGCCCTCCAGAAAAAGAATGCGCTCAata                                                                   <  1:4233837/93‑1 (MQ=255)

                       gAACGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAata                                                                   >  2:4482969/1‑93 (MQ=255)

                       gAACGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAata                                                                   <  2:2250933/93‑1 (MQ=255)

                       gAACGCAGCATTTCCTCAACATCGTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAata                                                                   <  2:2831782/93‑1 (MQ=255)

                         aCGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAAtata                                                                 >  2:7052162/1‑93 (MQ=255)

                         aCGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAAtata                                                                 >  1:3932059/1‑93 (MQ=255)

                         aCGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAAtata                                                                 <  1:2726879/93‑1 (MQ=255)

                         aCGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAAtata                                                                 <  2:7889782/93‑1 (MQ=255)

                          cGCATCATTTCCTCAACATCTTCACCAATGTTAAGTTTGCCCAGGTTCTGTAAGTTTGGAAGAACGTCCAGAAAAAGAATGCGCTCAATATaa                                                                >  2:464535/1‑93 (MQ=255)

                          cGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATaa                                                                <  2:7324194/93‑1 (MQ=255)

                          cGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATaa                                                                <  2:6171941/93‑1 (MQ=255)

                           gcagcaTTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAAc                                                               <  1:2628726/93‑1 (MQ=255)

                             agcaTTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGa                                                             >  1:2875079/1‑93 (MQ=255)

                             agcaTTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGa                                                             <  2:3026788/93‑1 (MQ=255)

                             agcaTTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGa                                                             <  1:6886048/93‑1 (MQ=255)

                              gcaTTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTTGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGAt                                                            <  2:3217890/93‑1 (MQ=255)

                              gcaTTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGAt                                                            >  2:493401/1‑93 (MQ=255)

                              gcaTTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGAt                                                            >  2:5863384/1‑93 (MQ=255)

                               cagttCCCCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGGCCCTCCAGAAAAAGAATGCGCTCAATATAACGATc                                                           <  2:7419461/90‑1 (MQ=255)

                               caTTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATc                                                           <  1:5194303/93‑1 (MQ=255)

                                aTTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCg                                                          <  2:3605120/93‑1 (MQ=255)

                                aTTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCg                                                          <  1:5805207/93‑1 (MQ=255)

                                aTTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCg                                                          <  1:7242820/93‑1 (MQ=255)

                                 tttCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCgg                                                         <  1:6148074/93‑1 (MQ=255)

                                      tCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCgtgt                                                    >  1:5707475/1‑93 (MQ=255)

                                       cAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGtt                                                   <  2:3106189/93‑1 (MQ=255)

                                       cAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGtt                                                   >  1:2236963/1‑93 (MQ=255)

                                        aaCATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGttt                                                  <  1:4530975/93‑1 (MQ=255)

                                        aaCATCTTCACCAATGTTCAGTTTGCACAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGttt                                                  >  2:2596403/1‑93 (MQ=255)

                                          cATCTTCACCAATGTTCCGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTtca                                                <  1:7832872/93‑1 (MQ=255)

                                          cATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTtca                                                >  2:138520/1‑93 (MQ=255)

                                          cATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTtca                                                <  1:1330075/93‑1 (MQ=255)

                                           aTCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTtcat                                               <  2:6674755/93‑1 (MQ=255)

                                            tCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTtcatc                                              >  2:1782091/1‑93 (MQ=255)

                                            tCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTtcatc                                              >  1:6516130/1‑93 (MQ=255)

                                             cTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTtcatct                                             >  2:5419267/1‑93 (MQ=255)

                                                cACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTtcatctcat                                          <  2:557474/93‑1 (MQ=255)

                                                 aCCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTtcatctcatc                                         >  1:3657/1‑93 (MQ=255)

                                                  ccAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCtcatca                                        >  1:5118702/1‑93 (MQ=255)

                                                   cAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCaa                                       >  2:5898382/1‑93 (MQ=255)

                                                   cAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCaa                                       <  2:3286051/93‑1 (MQ=255)

                                                     aTGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATg                                     <  1:6582878/93‑1 (MQ=255)

                                                       gTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGa                                   >  2:7622689/1‑93 (MQ=255)

                                                        ttCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGAt                                  <  1:1972140/93‑1 (MQ=255)

                                                          cAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTc                                >  2:8151667/1‑93 (MQ=255)

                                                           aGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCa                               >  1:6775670/1‑93 (MQ=255)

                                                             tttGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATg                             <  2:6051588/93‑1 (MQ=255)

                                                             tttGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATg                             >  2:914584/1‑93 (MQ=255)

                                                               tGCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGAt                           <  1:5085392/93‑1 (MQ=255)

                                                               tGCCCAGGTCCTGCAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGAt                           <  1:3743503/93‑1 (MQ=255)

                                                                gCCCAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGATa                          >  1:1562307/1‑93 (MQ=255)

                                                                  ccAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGATACt                        >  2:8731846/1‑93 (MQ=255)

                                                                   cAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGATACTc                       >  2:3973370/1‑93 (MQ=255)

                                                                   cAGGTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGATACTc                       <  1:3062258/93‑1 (MQ=255)

                                                                     ggTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGATACTCca                     >  2:5046120/1‑93 (MQ=255)

                                                                      gTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGATACTCcac                    >  1:1809165/1‑93 (MQ=255)

                                                                      gTCCTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGATACTCcac                    >  1:330113/1‑93 (MQ=255)

                                                                         cTGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGATACTCCAcatc                 >  1:5270295/1‑93 (MQ=255)

                                                                          tGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGATACTCCAcatca                >  2:4362025/1‑93 (MQ=255)

                                                                          tGTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGATACTCCAcatca                >  1:3445975/1‑93 (MQ=255)

                                                                           gTAAGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGATACTCCAcatcat               <  1:3129757/93‑1 (MQ=255)

                                                                              aGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGATACTCCACATCATTga            >  1:7955452/1‑93 (MQ=255)

                                                                              aGTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGATACTCCACATCATTga            <  1:6075614/93‑1 (MQ=255)

                                                                               gTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGATACTCCACATCATTgag           >  1:7376146/1‑93 (MQ=255)

                                                                               gTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGATACTCCACATCATTgag           >  2:3699766/1‑93 (MQ=255)

                                                                               gTTTGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGATACTCCACATCATTgag           <  2:6793341/93‑1 (MQ=255)

                                                                                 ttGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGATACTCCACATCATTGAGAc         <  1:8218503/93‑1 (MQ=255)

                                                                                 ttGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGATACTCCACATCATTGAGAc         <  1:6946626/93‑1 (MQ=255)

                                                                                 ttGGAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGATACTCCACATCATTGAGAc         <  1:7649494/93‑1 (MQ=255)

                                                                                   ggAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGATACTCCACATCATTGAGACGt       >  2:1980383/1‑93 (MQ=255)

                                                                                   ggAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGATACTCCACATCATTGAGACGt       >  2:2645763/1‑93 (MQ=255)

                                                                                    gAAGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGATACTCCACATCATTGAGACGtt      <  2:1683773/93‑1 (MQ=255)

                                                                                     aaGACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGATACTCCACATCATTGAGACGttt     <  2:5939160/93‑1 (MQ=255)

                                                                                       gACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGATACTCCACATCATTGAGACGTTTga   >  1:6221781/1‑93 (MQ=255)

                                                                                       gACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGATACTCCACATCATTGAGACGTTTga   >  1:919680/1‑93 (MQ=255)

                                                                                       gACCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGATACTCCACATCATTGAGACGTTTga   <  2:1173511/93‑1 (MQ=255)

                                                                                        aCCCTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGATACTCCACATCATTGAGACGTTTgag  <  2:8794366/93‑1 (MQ=255)

CATCCAGCTCAAGTGCCAGATCAGAACGCAGCATTTCCTCAACATCTTCACCAATGTTCAGTTTGCCCAGGTCCTGTAAGTTTGGAAGACCTTCCAGAAAAAGAATGCGCTCAATATAACGATCGGCGTGTTTCATCTCATCAATGGATTCATGATACTCCACATCATTGAGACGTTTGAG  >  NC_000913/3464074‑3464254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`