BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,629,781	A→G	V147A (GTT→GCT)	yhiJ ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,629,781	0	A	G	99.8%	1399.9 / ‑5.1	397	V147A (GTT→GCT)	yhiJ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (196/200); total (196/201)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.20e-01

Only 100 of 405 total aligned reads displayed.

GGTTTTATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAACATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAATTAATATCGGTATTATCAAGAGAGCAACCG  >  NC_000913/3629691‑3629870

ggTTTTATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTgcagca                                                                                         <  2:1104795/93‑1 (MQ=255)

  ttttATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGcatc                                                                                       >  2:3558914/1‑93 (MQ=255)

   tttATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGcatca                                                                                      <  1:8400502/93‑1 (MQ=255)

    ttATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGcatcat                                                                                     <  2:5218266/93‑1 (MQ=255)

    ttATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGcatcat                                                                                     <  2:3349255/93‑1 (MQ=255)

     tATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCAtt                                                                                    <  2:2170551/93‑1 (MQ=255)

      aTCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTa                                                                                   >  1:6522186/1‑93 (MQ=255)

       tCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAg                                                                                  >  2:5340248/1‑93 (MQ=255)

       tCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTCCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAg                                                                                  <  2:1294392/93‑1 (MQ=255)

         aTTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCa                                                                                >  2:3866784/1‑93 (MQ=255)

         aTTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGCAACTGCAGCATCATTAGCa                                                                                >  2:1793019/1‑93 (MQ=255)

           tGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCAtt                                                                              >  2:2986881/1‑93 (MQ=255)

            gTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTa                                                                             <  1:1263690/93‑1 (MQ=255)

            gTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTa                                                                             <  2:2741858/93‑1 (MQ=255)

            gTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTa                                                                             <  2:1346649/93‑1 (MQ=255)

            gTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTa                                                                             <  1:4641411/93‑1 (MQ=255)

            gTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTa                                                                             <  1:1724298/93‑1 (MQ=255)

             tttCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTAt                                                                            >  1:5733814/1‑93 (MQ=255)

              ttCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATc                                                                           >  2:3799821/1‑93 (MQ=255)

                 gAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCAtt                                                                        >  1:376815/1‑93 (MQ=255)

                 gAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCAtt                                                                        >  1:7911506/1‑93 (MQ=255)

                 gAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCAtt                                                                        <  1:5018063/93‑1 (MQ=255)

                  aaTTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCAttt                                                                       <  2:4583367/93‑1 (MQ=255)

                  aaTTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCAttt                                                                       <  2:7348958/93‑1 (MQ=255)

                  aaTTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGCGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCAttt                                                                       <  1:3521638/93‑1 (MQ=255)

                   aTTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTa                                                                      <  2:3502695/93‑1 (MQ=255)

                   aTTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTa                                                                      <  1:5996207/93‑1 (MQ=255)

                     ttAATATCAGATACATTATATTTCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACt                                                                    <  2:4027229/93‑1 (MQ=255)

                     ttAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCGGCATCATTAGCATTATCATTTACt                                                                    <  1:4464130/93‑1 (MQ=255)

                     ttAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACt                                                                    <  1:6826148/93‑1 (MQ=255)

                     ttAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACt                                                                    >  1:7027623/1‑93 (MQ=255)

                      tAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTg                                                                   <  2:4095857/93‑1 (MQ=255)

                        atatCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTc                                                                 >  1:3662373/1‑93 (MQ=255)

                         tatCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCGTCATTAGCATTATCATTTACTGTCa                                                                >  1:5069820/1‑93 (MQ=255)

                         tatCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCa                                                                >  1:5504807/1‑93 (MQ=255)

                          atCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCat                                                               >  1:2336115/1‑93 (MQ=255)

                           tCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCata                                                              <  1:3105520/93‑1 (MQ=255)

                           tCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCata                                                              >  2:4412516/1‑93 (MQ=255)

                           tCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCata                                                              <  2:8326803/93‑1 (MQ=255)

                           tCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCata                                                              >  1:4005863/1‑93 (MQ=255)

                            cAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCatat                                                             >  2:656148/1‑93 (MQ=255)

                             aGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCAtatt                                                            >  1:151361/1‑93 (MQ=255)

                              gATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCAtatta                                                           <  2:3671372/93‑1 (MQ=255)

                              gATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCAtatta                                                           <  2:8134941/93‑1 (MQ=255)

                              gATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCAtatta                                                           >  1:8820909/1‑93 (MQ=255)

                               aTACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCAtattat                                                          <  1:5882545/93‑1 (MQ=255)

                                 aCATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATAttatta                                                        <  1:179703/93‑1 (MQ=255)

                                   aTTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAg                                                      >  2:6143259/1‑93 (MQ=255)

                                   aTTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAg                                                      >  1:2186483/1‑93 (MQ=255)

                                   aTTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAg                                                      >  2:419288/1‑93 (MQ=255)

                                   aTTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAg                                                      >  1:3361700/1‑93 (MQ=255)

                                    ttATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGt                                                     <  1:1353725/93‑1 (MQ=255)

                                    ttATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTAGCATTTACTGTCATATTATTAAGt                                                     >  2:6286332/1‑93 (MQ=255)

                                     tataTTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGta                                                    >  1:1944424/1‑93 (MQ=255)

                                       tatTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGtaaa                                                  <  1:4546577/93‑1 (MQ=255)

                                       tatTGCGATATTTTCCAGTCCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGtaaa                                                  <  1:8572558/93‑1 (MQ=255)

                                         ttGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGtaaata                                                >  2:344374/1‑93 (MQ=255)

                                           gCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGtaaataaa                                              <  1:722775/93‑1 (MQ=255)

                                              atatTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACaa                                           >  1:7842895/1‑93 (MQ=255)

                                               tatTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAAt                                          <  1:7181143/93‑1 (MQ=255)

                                                atTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATa                                         >  2:3129911/1‑93 (MQ=255)

                                                   ttccagttccagATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTg                                      >  2:6655577/1‑93 (MQ=255)

                                                    tccagttccagATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGc                                     >  2:1648488/1‑93 (MQ=255)

                                                    tccagttccagATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGc                                     >  2:1405879/1‑93 (MQ=255)

                                                     ccagttccagATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCa                                    >  2:57872/1‑93 (MQ=255)

                                                      cagttccagATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCAc                                   >  2:8098542/1‑93 (MQ=255)

                                                      cagttccagATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCAc                                   >  1:2731212/1‑93 (MQ=255)

                                                       agttccagATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCAcc                                  >  1:4808348/1‑93 (MQ=255)

                                                       agttccagATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAAAAATACTGCAcc                                  >  2:7020557/1‑93 (MQ=255)

                                                        gttccagATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCa                                 >  1:5543956/1‑93 (MQ=255)

                                                        gttccagATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCa                                 >  1:1093463/1‑93 (MQ=255)

                                                        gttccagATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCa                                 <  1:2930217/93‑1 (MQ=255)

                                                           ccagATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAAtt                              <  1:5409967/93‑1 (MQ=255)

                                                            cagATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAATTa                             >  2:2288383/1‑93 (MQ=255)

                                                            cagATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAATTa                             <  1:7702054/93‑1 (MQ=255)

                                                             agATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAATTaa                            >  1:5825628/1‑93 (MQ=255)

                                                             agATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAATTaa                            >  1:6223493/1‑93 (MQ=255)

                                                              gATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAATTAat                           >  1:757672/1‑93 (MQ=255)

                                                              gATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAATTAat                           <  2:2258661/93‑1 (MQ=255)

                                                              gATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAATTAat                           <  1:6642786/93‑1 (MQ=255)

                                                              gATGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAATTAat                           >  2:6163986/1‑93 (MQ=255)

                                                               aTGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAATTAata                          >  1:8166065/1‑93 (MQ=255)

                                                                tGGGTTTGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAATTAatat                         <  2:717709/93‑1 (MQ=255)

                                                                     ttGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAATTAATATCGGta                    <  2:8411419/93‑1 (MQ=255)

                                                                     ttGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAATTAATATCGGta                    <  2:6448272/93‑1 (MQ=255)

                                                                      tGTACGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAATTAATATCGGtat                   <  2:2527222/93‑1 (MQ=255)

                                                                         aCGACTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAATTAATATCGGtattat                <  2:8485961/93‑1 (MQ=255)

                                                                            aCTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAATTAATATCGGTATTATCaa             >  1:2452334/1‑93 (MQ=255)

                                                                            aCTCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAATTAATATCGGTATTATCaa             >  2:2049403/1‑93 (MQ=255)

                                                                              tCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAATTAATATCGGTATTATCAaga           <  2:7548617/93‑1 (MQ=255)

                                                                              tCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAATTAATATCGGTATTATCAaga           >  2:8732283/1‑93 (MQ=255)

                                                                              tCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAATTAATATCGGTATTATCAaga           <  1:8014661/93‑1 (MQ=255)

                                                                              tCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAATTAATATCGGTATTATCAaga           <  2:5123238/93‑1 (MQ=255)

                                                                              tCAGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAATTAATATCGGTATTATCAaga           <  2:4748496/93‑1 (MQ=255)

                                                                                aGTAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAATTAATATCGGTATTATCAagaga         <  2:6730859/93‑1 (MQ=255)

                                                                                  tAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAATTAATATCGGTATTATCAAGAGAGc       <  1:3585195/93‑1 (MQ=255)

                                                                                  tAACTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAATTAATATCGGTATTATCAAGAGAGc       <  2:5561381/93‑1 (MQ=255)

                                                                                    aCTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAATTAATATCGGTATTATCAAGAGAGCaa     <  2:7897326/93‑1 (MQ=255)

                                                                                     cTGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAATTAATATCGGTATTATCAAGAGAGCAAc    <  2:1957290/93‑1 (MQ=255)

                                                                                      tGCAGCATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAATTAATATCGGTATTATCAAGAGAGCAAcc   >  2:5939441/1‑93 (MQ=255)

GGTTTTATCATTGTTTCGAATTTAATATCAGATACATTATATTGCGATATTTTCCAGTTCCAGATGGGTTTGTACGACTCAGTAACTGCAACATCATTAGCATTATCATTTACTGTCATATTATTAAGTAAATAAACAATACTGCACCAATTAATATCGGTATTATCAAGAGAGCAACCG  >  NC_000913/3629691‑3629870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`