BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,764,283	T→C	V107A (GTT→GCT)	yibA →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,764,283	0	T	C	99.3%	1345.4 / ‑4.0	407	V107A (GTT→GCT)	yibA	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (191/213); total (193/214)
Fisher's exact test for biased strand distribution p-value = 6.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 421 total aligned reads displayed.

TACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACTG  >  NC_000913/3764193‑3764372

taCTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTc                                                                                         <  2:2479717/93‑1 (MQ=255)

taCTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTc                                                                                         <  1:6154634/93‑1 (MQ=255)

 aCTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCg                                                                                        >  2:7780917/1‑93 (MQ=255)

      ggCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTa                                                                                   <  1:1634579/93‑1 (MQ=255)

       gCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTAc                                                                                  <  1:6537875/93‑1 (MQ=37)

        cAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACg                                                                                 >  1:8529777/1‑93 (MQ=255)

         aaaTAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACgg                                                                                <  1:7026805/93‑1 (MQ=255)

         aaaTAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACgg                                                                                >  2:6756634/1‑93 (MQ=255)

          aaTAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGc                                                                               >  2:4901447/1‑93 (MQ=37)

               aaaTTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCg                                                                          <  1:442336/93‑1 (MQ=21)

               aaaTTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCg                                                                          <  2:6042291/93‑1 (MQ=18)

               aaaTTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCg                                                                          <  2:7371678/93‑1 (MQ=21)

                aaTTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGa                                                                         <  1:980053/93‑1 (MQ=18)

                 aTTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAg                                                                        >  1:4326605/1‑93 (MQ=18)

                   ttGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTc                                                                      <  1:8268824/93‑1 (MQ=21)

                     gCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCaa                                                                    <  1:1921429/93‑1 (MQ=18)

                      cAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAAc                                                                   <  2:5372326/93‑1 (MQ=21)

                      cAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAAc                                                                   <  2:4249353/93‑1 (MQ=18)

                       aaaaaaTGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACg                                                                  >  1:1357609/1‑93 (MQ=18)

                        aaaaaTGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACgg                                                                 >  1:2975169/1‑93 (MQ=18)

                         aaaaTGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGc                                                                <  2:2415252/93‑1 (MQ=21)

                         aaaaTGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGc                                                                >  2:1259090/1‑93 (MQ=21)

                           aaTGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTc                                                              >  1:4878770/1‑93 (MQ=25)

                           aaTGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTc                                                              <  1:1845126/93‑1 (MQ=25)

                            aTGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCa                                                             >  2:7834827/1‑93 (MQ=37)

                             tGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAg                                                            <  2:5109737/93‑1 (MQ=255)

                             tGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAg                                                            >  1:1447045/1‑93 (MQ=255)

                              gCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGc                                                           <  1:2092442/93‑1 (MQ=255)

                               cGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCg                                                          >  1:1110058/1‑93 (MQ=255)

                                gAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGa                                                         <  2:2865718/93‑1 (MQ=255)

                                 aaGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGAt                                                        >  1:3520877/1‑93 (MQ=255)

                                 aaGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTGGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGAt                                                        <  2:3757889/93‑1 (MQ=255)

                                   gATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGc                                                      <  2:8790135/93‑1 (MQ=255)

                                     tAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCaa                                                    <  1:6415060/93‑1 (MQ=255)

                                     tAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCaa                                                    >  1:4778429/1‑93 (MQ=255)

                                     tAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCaa                                                    <  1:8734518/93‑1 (MQ=255)

                                     tAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAACTACGGCAATCGAGTCAACGGCTCAGCGATGCaa                                                    >  1:5363708/1‑93 (MQ=255)

                                     ataTGTTGGTAATATTGTGAACAATAGGGCATTGAACGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCTAGTCAACTGCTCAGCGATGCaa                                                    <  1:4537134/91‑1 (MQ=17)

                                      aaTGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAg                                                   >  1:1232170/1‑93 (MQ=255)

                                      aaTGTTTTTAATATTTTGAACAATAT‑GCATTGCATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATTGAGTCAACGCCTCAGCGAGGCAAGa                                                  >  2:4516650/1‑93 (MQ=14)

                                       aTGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGa                                                  <  2:4824213/93‑1 (MQ=255)

                                        tGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGaa                                                 >  2:3027301/1‑93 (MQ=255)

                                        tGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGaa                                                 >  2:7518433/1‑93 (MQ=255)

                                        tGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGaa                                                 >  2:6686936/1‑93 (MQ=255)

                                         gTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGaaa                                                >  2:6618237/1‑93 (MQ=255)

                                          tttttAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGaaaa                                               >  2:8080598/1‑93 (MQ=255)

                                           ttttAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGaaaaa                                              >  1:6625819/1‑93 (MQ=255)

                                            tttAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAAc                                             >  2:3304762/1‑93 (MQ=255)

                                             ttAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAAcc                                            >  2:8573884/1‑93 (MQ=255)

                                                atatTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCaa                                         >  2:2583452/1‑93 (MQ=255)

                                                 tatTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAAt                                        <  1:5020647/93‑1 (MQ=255)

                                                 tatTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAAt                                        <  1:1769044/93‑1 (MQ=255)

                                                 tatTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAAt                                        >  1:2807540/1‑93 (MQ=255)

                                                  atTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAAtt                                       <  1:2202834/93‑1 (MQ=255)

                                                   ttttGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAAttt                                      <  1:413766/93‑1 (MQ=255)

                                                    tttGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCTACGGCTCAGCGATGCAAGAAAAACCCAATTTa                                     >  2:4591953/1‑93 (MQ=255)

                                                    tttGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTa                                     <  2:4756527/93‑1 (MQ=255)

                                                       gAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTc                                  >  2:934029/1‑93 (MQ=255)

                                                        aaCAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCa                                 <  1:3530819/93‑1 (MQ=255)

                                                         aCAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCAc                                >  1:4072845/1‑93 (MQ=255)

                                                         aCAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCAc                                >  2:6979888/1‑93 (MQ=255)

                                                          cAATATGGCATTGAATAATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCAcc                               <  1:1559965/93‑1 (MQ=255)

                                                           aaTATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCt                              <  2:2715336/93‑1 (MQ=255)

                                                           aaTATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCt                              >  2:3887014/1‑93 (MQ=255)

                                                             tttGGTATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCATCGGCTCAGCGTTGCAAGAAATACCCAATTTATTCACCTaa                            >  1:7805918/3‑93 (MQ=21)

                                                             tatGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTaa                            >  1:2493434/1‑93 (MQ=255)

                                                             tatGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTaa                            >  2:1855486/1‑93 (MQ=255)

                                                             aatGGCATTGAATGATAAGAGCGCGTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTaa                            <  2:6461904/92‑1 (MQ=255)

                                                               tGGCATTGATTGATAAGAGCCCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACACAATTTATTCCCCTaaaa                          <  2:4122780/93‑1 (MQ=25)

                                                               tGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTaaaa                          <  2:5803609/93‑1 (MQ=255)

                                                                 gCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAAta                        <  1:5234334/93‑1 (MQ=255)

                                                                 gCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAAta                        <  2:3521090/93‑1 (MQ=255)

                                                                  cATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAAtag                       >  2:7658942/1‑93 (MQ=255)

                                                                  cATTGAATAATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAAtag                       <  1:3208246/93‑1 (MQ=255)

                                                                    ttGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAAtagta                     >  1:8084610/1‑93 (MQ=255)

                                                                     tGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAAtagtag                    >  1:7589972/1‑93 (MQ=255)

                                                                     tGAATGATAAGACGGCTTGCGCTCGATCTCCGGCAATCGAGTCACCGACTCAACCATGCAAGACAATCCCAATTTACTTACCTAAAAtagtgc                    >  2:6114855/1‑91 (MQ=16)

                                                                        aTGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAAc                 >  1:3937251/1‑93 (MQ=255)

                                                                        aTGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTACAAc                 >  2:3126163/1‑93 (MQ=255)

                                                                          gATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACaa               <  1:3855132/93‑1 (MQ=255)

                                                                            tAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAAtc             <  2:5097888/93‑1 (MQ=255)

                                                                            tAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAAtc             <  1:604386/93‑1 (MQ=255)

                                                                             aaGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAAtct            >  1:7209262/1‑93 (MQ=255)

                                                                              agagCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAAtctc           <  1:3052401/93‑1 (MQ=255)

                                                                               gagCGCTTGCGCTCGAGCTACGGCAATCGAGGCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCa          >  2:5189534/1‑93 (MQ=255)

                                                                                agCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCaa         >  2:1607909/1‑93 (MQ=255)

                                                                                 gcgcTTGCGCTCGAGGTACGGCAATCGAGTCAACGGCTCAGCGATGCAAAAAAAACCCAATTTATTCCCCTAAAATAGTAGAACAATCTCaaa        <  2:5694199/93‑1 (MQ=38)

                                                                                 gcgcTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCaaa        <  1:3684170/93‑1 (MQ=255)

                                                                                  cgcTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAAt       <  1:6923225/93‑1 (MQ=255)

                                                                                  cgcTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAAt       >  1:7532250/1‑93 (MQ=255)

                                                                                    cTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTa     <  2:222295/93‑1 (MQ=255)

                                                                                    cTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTa     >  1:5971289/1‑93 (MQ=255)

                                                                                    cTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTa     >  1:262553/1‑93 (MQ=255)

                                                                                    cTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTa     >  1:7700942/1‑93 (MQ=255)

                                                                                     ttGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTAc    <  1:3781630/93‑1 (MQ=255)

                                                                                     ttGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTAc    <  1:5688550/93‑1 (MQ=255)

                                                                                      tGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACt   <  2:5942632/93‑1 (MQ=255)

                                                                                      tGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACt   >  1:8666399/1‑93 (MQ=255)

                                                                                      tGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACt   <  1:7406913/93‑1 (MQ=255)

                                                                                       gcgcTCGAGCTACGGCAATCGAGTCAACGGCTTAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACta  <  1:1002652/93‑2 (MQ=255)

TACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACTG  >  NC_000913/3764193‑3764372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`