BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,853,551	T→C	Q16R (CAG→CGG)	yidG ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,853,551	0	T	C	99.7%	1200.4 / ‑5.2	349	Q16R (CAG→CGG)	yidG	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (154/194); minor base A (0/1); total (154/195)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.63e-01

Only 100 of 361 total aligned reads displayed.

GCCTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATAT  >  NC_000913/3853461‑3853640

 ccTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCa                                                                                        >  1:1304641/1‑93 (MQ=255)

 ccTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCa                                                                                        <  1:4865674/93‑1 (MQ=255)

 ccTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCa                                                                                        <  1:3708511/93‑1 (MQ=255)

  cTGGTGCCAGTTGTGTTTGATAGCCAGCGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAg                                                                                       <  1:3620541/93‑1 (MQ=255)

   tGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGt                                                                                      >  2:7195227/1‑93 (MQ=255)

    ggTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTc                                                                                     >  2:1068641/1‑93 (MQ=255)

     gTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTcc                                                                                    <  1:8314101/93‑1 (MQ=255)

     gTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTcc                                                                                    <  1:6769300/93‑1 (MQ=255)

     gTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTcc                                                                                    >  1:5388954/1‑93 (MQ=255)

     gTGCCAATTGTGTTTGATAGCCAACGACATCAACGCGCCGTAGCGCAGCACGGCACGAAACCCCGCCAGTGATGTGCGCTCGGGCAGCAGGcc                                                                                    >  2:4236880/1‑93 (MQ=255)

      tGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTccg                                                                                   >  2:2007509/1‑92 (MQ=255)

       gCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTg                                                                                  <  2:724146/93‑1 (MQ=255)

       gCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTg                                                                                  <  1:1232951/93‑1 (MQ=255)

       gCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTg                                                                                  >  2:2515191/1‑93 (MQ=255)

          aGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGt                                                                               <  2:4695186/93‑1 (MQ=255)

           gTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTc                                                                              <  1:8178802/93‑1 (MQ=255)

            ttGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCg                                                                             >  1:8387851/1‑93 (MQ=255)

            ttGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCg                                                                             >  1:8660863/1‑93 (MQ=255)

             tgtgTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCgg                                                                            >  1:7994242/1‑93 (MQ=255)

              ttgtTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGc                                                                           <  2:7458358/92‑1 (MQ=255)

               tgtTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCa                                                                          <  2:3599902/93‑1 (MQ=255)

                ttttGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCaa                                                                         <  2:7405682/92‑1 (MQ=255)

                gtTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCaa                                                                         <  1:1962622/93‑1 (MQ=255)

                gtTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCaa                                                                         <  2:5480082/93‑1 (MQ=255)

                 tttGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAAt                                                                        <  2:6952766/93‑1 (MQ=255)

                  ttGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATg                                                                       <  2:3261368/93‑1 (MQ=255)

                    gATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCg                                                                     >  1:6128629/1‑93 (MQ=255)

                    gATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCg                                                                     >  1:6313448/1‑93 (MQ=255)

                      tAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTc                                                                   <  1:6908172/93‑1 (MQ=255)

                      tAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTc                                                                   <  2:4919866/93‑1 (MQ=255)

                      aaGCCACCGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTc                                                                   <  2:7792017/92‑1 (MQ=255)

                        gCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTg                                                                 <  1:2290470/93‑1 (MQ=255)

                        gCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTg                                                                 >  2:2850572/1‑93 (MQ=255)

                          cAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGc                                                               >  2:8207818/1‑93 (MQ=255)

                           aaCGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCt                                                              >  2:1344178/1‑93 (MQ=255)

                           aaCGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCt                                                              <  2:6728894/93‑1 (MQ=255)

                             cGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCttt                                                            <  2:3101138/93‑1 (MQ=255)

                              gCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTg                                                           <  1:3231705/93‑1 (MQ=255)

                               ccATCAGCGCGCCGTAGCCCAGCATGGTACTAAACCACGCCAGTGATGTGCGCTCCGTCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGc                                                          <  1:1457531/93‑1 (MQ=255)

                               ccATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGc                                                          <  2:4381389/93‑1 (MQ=255)

                               ccATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGc                                                          >  1:4265298/1‑93 (MQ=255)

                               ccATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGc                                                          <  1:3827014/93‑1 (MQ=255)

                                cATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCg                                                         <  1:5580651/93‑1 (MQ=255)

                                 aTCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCgg                                                        >  2:1676963/1‑93 (MQ=255)

                                 aTCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCgg                                                        >  2:246949/1‑93 (MQ=255)

                                 aTCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCgg                                                        >  1:3348971/1‑93 (MQ=255)

                                  tCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCATTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGc                                                       >  1:7501030/1‑93 (MQ=255)

                                   cAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCt                                                      <  2:4268204/93‑1 (MQ=255)

                                    aaCGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTa                                                     >  2:8521064/1‑93 (MQ=255)

                                    aaCGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTa                                                     >  1:5685028/1‑93 (MQ=255)

                                    aaCGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTa                                                     >  1:304654/1‑93 (MQ=255)

                                     aCGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTAt                                                    >  1:7195483/1‑93 (MQ=255)

                                     aCGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTAt                                                    >  1:350648/1‑93 (MQ=255)

                                     aCGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCCAt                                                    >  2:5870063/1‑93 (MQ=255)

                                       gcgcCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCCGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATcc                                                  <  1:732966/93‑1 (MQ=255)

                                       gcgcCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATcc                                                  <  2:2613998/93‑1 (MQ=255)

                                       gcgcCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATcc                                                  <  2:6497626/93‑1 (MQ=255)

                                         gcCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCgg                                                >  2:7996190/1‑93 (MQ=255)

                                           cGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCa                                              <  1:2076998/93‑1 (MQ=255)

                                           cGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCAGCAATGCGTCTGGCTTTGCGGCTATCCGGCa                                              >  2:6116863/1‑93 (MQ=255)

                                            gTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCAt                                             >  1:3986417/1‑93 (MQ=255)

                                            gTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCAt                                             >  1:4199209/1‑93 (MQ=255)

                                             tAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATa                                            <  2:7673885/93‑1 (MQ=255)

                                             tAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATa                                            >  1:1038566/1‑93 (MQ=255)

                                             tAGCCCAGCATGGTACGAAACCAAGCCAGAGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGAGGCTATCCGCCAtc                                            >  1:2413295/1‑92 (MQ=255)

                                              aGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATac                                           >  1:5891442/1‑93 (MQ=255)

                                               gCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATaca                                          >  2:8311932/1‑93 (MQ=255)

                                                 ccAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATacaac                                        >  2:2208251/1‑93 (MQ=255)

                                                  cAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATacaaca                                       <  2:3973241/93‑1 (MQ=255)

                                                    gCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACANCAcc                                     <  1:6271680/93‑1 (MQ=255)

                                                      aTGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAg                                   <  1:3819002/93‑1 (MQ=255)

                                                      aTGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAg                                   >  1:956212/1‑93 (MQ=255)

                                                       tGGCACGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATGCGGCATACAACACCAgc                                  >  2:873336/1‑92 (MQ=255)

                                                           aCGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAAGACAAGTCCCa                              >  2:3953553/1‑93 (MQ=255)

                                                            cGAAACCACGCCAGTGATGTGCGCTCCGTCCGCAGTCCTGGGTCTGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCAt                             <  1:7673883/93‑1 (MQ=255)

                                                            cGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCAt                             <  2:1142333/93‑1 (MQ=255)

                                                            cGAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCAt                             >  2:5229121/1‑93 (MQ=255)

                                                             gAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATa                            <  2:86146/93‑1 (MQ=255)

                                                             gAAACCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATa                            >  2:181344/1‑93 (MQ=255)

                                                              aaaCCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATaa                           >  1:1105073/1‑93 (MQ=255)

                                                               aaCCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATAAc                          <  1:454502/93‑1 (MQ=255)

                                                                aCCACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATAACg                         >  1:2742556/1‑93 (MQ=255)

                                                                  cACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATAACGAt                       >  2:6296472/1‑93 (MQ=255)

                                                                  cACGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATAACGAt                       <  1:2893599/93‑1 (MQ=255)

                                                                   aCGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATAACGATc                      >  2:7878215/1‑93 (MQ=255)

                                                                    cGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATAACGATCa                     <  2:4480597/93‑1 (MQ=255)

                                                                    cGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATAACGATCa                     <  1:2506766/93‑1 (MQ=255)

                                                                    cGCCAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATAACGATCa                     <  1:5105491/93‑1 (MQ=255)

                                                                     ggcAGTGATGTGCCCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATAACGATCAc                    <  1:1915222/91‑1 (MQ=255)

                                                                       cAGTGATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATAACGATCAccc                  >  1:4507891/1‑92 (MQ=255)

                                                                           gATGTGCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCAc              <  1:7770796/93‑1 (MQ=255)

                                                                             tgtgCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCATTCCCATAACGATCACCGCCACGa            >  2:4798871/1‑93 (MQ=255)

                                                                             tgtgCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGa            <  1:8274235/93‑1 (MQ=255)

                                                                              gtgCGCTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGAc           >  2:151598/1‑93 (MQ=255)

                                                                                 cgcTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCAt        >  2:3584207/1‑93 (MQ=255)

                                                                                   cTCCGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCa      >  1:3195600/1‑93 (MQ=255)

                                                                                     ccGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAat    >  1:6355890/1‑93 (MQ=255)

                                                                                     ccGGCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAat    <  1:6614877/93‑1 (MQ=255)

                                                                                      cGGCCGAAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGAATACAACAACAGTCCCATAACGATCTCCGCCACGACCATCaaga   <  2:5515353/93‑3 (MQ=255)

                                                                                       ggCCGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAatat  >  2:3766768/1‑93 (MQ=255)

GCCTGGTGCCAGTTGTGTTTGATAGCCAACGCCATCAACGCGCCGTAGCCCAGCATGGTACGAAACCACGCCAGTGATGTGCGCTCCGGCTGCAGTCCTGGGTCGGCAATGCGTCTGGCTTTGCGGCTATCCGGCATACAACACCAGTCCCATAACGATCACCGCCACGACCATCAATAT  >  NC_000913/3853461‑3853640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`