BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,878,432	A→G	C140R (TGC→CGC)	recF ←	RecF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,878,432	0	A	G	99.5%	1223.8 / ‑3.6	366	C140R (TGC→CGC)	recF	RecF
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (170/194); total (171/195)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.73e-01

Only 100 of 383 total aligned reads displayed.

ACGTGTCACCTGGCGCAGCGCCGCATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCATCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGT  >  NC_000913/3878340‑3878521

  gtgtCACCTGGCGCAGCGCCGCATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTc                                                                                         >  1:5130057/1‑93 (MQ=255)

  gtgtCACCTGGCGCAGCGCCGCATTGCGCTGCTTAAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTc                                                                                         >  2:4336124/1‑93 (MQ=255)

   tgtCACCTGGCGCAGCGCCGCATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTcc                                                                                        <  2:1678590/93‑1 (MQ=255)

   tgtCACCTGGCGCAGCGCCGCATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTcc                                                                                        <  2:224314/93‑1 (MQ=255)

    gtCACCTGGCGCAGCGCCGCATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTccc                                                                                       <  2:1848105/93‑1 (MQ=255)

     tCACCTGGCGCAGCGCCGCATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTcccc                                                                                      <  2:1283291/93‑1 (MQ=255)

       aCCTGGCGCAGCGCCGCATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAg                                                                                    >  1:2347762/1‑93 (MQ=255)

         caggCGCAGCGCCGCATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTc                                                                                  <  2:8054948/91‑1 (MQ=255)

           ggCGCAGCGCCGCATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGa                                                                                <  1:8377017/93‑1 (MQ=255)

           ggCGCAGCGCCGCATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGa                                                                                >  1:1937594/1‑93 (MQ=255)

            gcgcAGCGCCGCGTTGCGCTGCGTGAGAAATCACTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAg                                                                               <  2:6221659/93‑1 (MQ=255)

            gcgcAGCGCCGCATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAg                                                                               >  1:4927237/1‑93 (MQ=255)

              gcAGCGCCGCATTGCGCTGCTTGAGCAATCGATTGAGATTGCTCCAGGCGGTGAAAAATCCGGGGTCGTTGTGCAAGCGTCCCCAGGCGAGGa                                                                             >  1:108740/1‑93 (MQ=255)

              gcAGCGCCGCATTGCGCTGCTTAAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGa                                                                             >  1:1190364/1‑93 (MQ=255)

               cAGCGCCGCATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGaa                                                                            >  1:2945642/1‑93 (MQ=255)

               cAGCGCCGCATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGaa                                                                            >  1:6173169/1‑93 (MQ=255)

                 gcgcCGCATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCTGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATg                                                                          >  1:2696497/1‑93 (MQ=255)

                   gccgcATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGGTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGct                                                                        >  1:7788024/1‑93 (MQ=255)

                    ccgcATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGctc                                                                       >  1:3604383/1‑93 (MQ=255)

                     cgcATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGctct                                                                      >  2:5645870/1‑93 (MQ=255)

                     cgcATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGctct                                                                      >  2:8554336/1‑93 (MQ=255)

                     cgcATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGctct                                                                      >  1:95669/1‑93 (MQ=255)

                     cgcATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGctct                                                                      >  1:8206915/1‑93 (MQ=255)

                      gcATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCtctt                                                                     >  1:3236660/1‑93 (MQ=255)

                       cATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCtcttc                                                                    <  2:3490660/93‑1 (MQ=255)

                       cATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCtcttc                                                                    <  1:156601/93‑1 (MQ=255)

                        aTTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCtcttct                                                                   >  1:5609214/1‑93 (MQ=255)

                        aTTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCtcttat                                                                   >  1:5262256/1‑91 (MQ=255)

                          tGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGt                                                                 <  2:4071365/93‑1 (MQ=255)

                           gcgcTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTa                                                                <  2:1087971/93‑1 (MQ=255)

                             gctgctTGAGCAATAGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTAtt                                                              >  2:2021430/1‑93 (MQ=255)

                                gctTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTgg                                                           <  1:4651110/93‑1 (MQ=255)

                                 ctTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTggg                                                          <  2:5830330/93‑1 (MQ=255)

                                  ttGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAgg                                                                              >  2:5591898/1‑72 (MQ=255)

                                   tGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGgc                                                        <  2:2979921/93‑1 (MQ=255)

                                    gAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGgcc                                                       >  1:7506804/1‑93 (MQ=255)

                                    gAGCAATCGCTTGAGATTGCTCCAGGCGGTAAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCCTCTGTATTTGGGgcc                                                       <  2:4037770/93‑1 (MQ=255)

                                      gaagtCGCTTGAGTTTCCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGgccgc                                                     <  2:1580347/89‑1 (MQ=255)

                                       cAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGgccgcc                                                    <  2:8719207/93‑1 (MQ=255)

                                        aaTCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGccgccg                                                   >  1:1040267/1‑93 (MQ=255)

                                         aTCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGt                                                  >  1:7245358/1‑93 (MQ=255)

                                         aTCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGt                                                  <  1:4404944/93‑1 (MQ=255)

                                         aTCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGt                                                  >  2:5101125/1‑93 (MQ=255)

                                          tCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTATATTTGGGGCCGCCGtt                                                 >  1:5923198/1‑93 (MQ=255)

                                           cGCTCGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTg                                                <  1:8558564/93‑1 (MQ=255)

                                            gCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGa                                               <  2:8164225/93‑1 (MQ=255)

                                            gCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGa                                               >  1:1307450/1‑93 (MQ=255)

                                             cTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGag                                              <  1:3464857/93‑1 (MQ=255)

                                              ttGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGagt                                             >  1:6621359/1‑93 (MQ=255)

                                              ttGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGagt                                             >  1:2263920/1‑93 (MQ=255)

                                                 agaTTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGagtaaa                                          <  1:237810/93‑1 (MQ=255)

                                                 agaTTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGagtaaa                                          >  1:7304830/1‑93 (MQ=255)

                                                  gaTTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGagtaaag                                         <  1:3012885/93‑1 (MQ=255)

                                                   aTTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGagtaaagt                                        <  2:5877015/93‑1 (MQ=255)

                                                   aTTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCAAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGagtaaaga                                        >  1:6996171/1‑92 (MQ=255)

                                                    ttGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGagtaaagta                                       >  1:6248452/1‑93 (MQ=255)

                                                    gtgatCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGagtaaagta                                       <  2:4218965/89‑1 (MQ=255)

                                                      gCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAgtaaagtaaa                                     <  1:3145580/93‑1 (MQ=255)

                                                       cgccAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAAc                                    <  2:65552/91‑1 (MQ=255)

                                                       cTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAAc                                    >  2:7977245/1‑93 (MQ=255)

                                                       cTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAAc                                    <  1:3762526/93‑1 (MQ=255)

                                                       cTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAAc                                    <  1:4211506/93‑1 (MQ=255)

                                                       cTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAAc                                    <  1:8113638/93‑1 (MQ=255)

                                                        tCCAGGCGGTGCAAACTCCGGGTTCGTTGTGAAAGCGAAAACAAACGAGGTCTGCTCTTCAGTTTTAGGGACCGCCGTTGCGTAAAGTAAccc                                   <  1:7838902/93‑1 (MQ=255)

                                                         ccAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAAccc                                  <  1:575164/93‑1 (MQ=255)

                                                         ccAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAAccc                                  >  1:2214381/1‑93 (MQ=255)

                                                          cAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCt                                 >  2:8453980/1‑93 (MQ=255)

                                                          cAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCt                                 <  1:3377142/93‑1 (MQ=255)

                                                          cAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCt                                 >  2:8675581/1‑93 (MQ=255)

                                                            ggCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTc                               >  2:4525308/1‑93 (MQ=255)

                                                             gCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCt                              <  2:2621595/93‑1 (MQ=255)

                                                             gCGGTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGAAAAGTAAACCCTTcg                              >  1:6702894/1‑92 (MQ=255)

                                                                gTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGc                           >  2:1521871/1‑93 (MQ=255)

                                                                gTGAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGc                           <  2:7649088/93‑1 (MQ=255)

                                                                  gAAAAATCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGt                         <  1:4893967/93‑1 (MQ=255)

                                                                   aaaaaTCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGtt                        <  1:8731244/93‑1 (MQ=255)

                                                                    aaaaTCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTa                       >  1:4355054/1‑93 (MQ=255)

                                                                    aaaaTCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTa                       <  1:8514800/93‑1 (MQ=255)

                                                                    aaaaTCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGGTa                       >  2:1989159/1‑93 (MQ=255)

                                                                     aaaTCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTAt                      >  2:518085/1‑93 (MQ=255)

                                                                      aaTCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATc                     <  2:753629/93‑1 (MQ=255)

                                                                      aaTCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCCCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATc                     >  1:6644333/1‑93 (MQ=255)

                                                                        tCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCaa                   <  2:8278964/93‑1 (MQ=255)

                                                                        tCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCaa                   <  1:7671416/93‑1 (MQ=255)

                                                                        tCCGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGGTGAGTAAAGTAAACCCTTCTGGCGTTATCaa                   <  2:4712108/93‑1 (MQ=255)

                                                                          cGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACt                 >  2:4833302/1‑93 (MQ=255)

                                                                          cGGGTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACt                 <  2:3376326/93‑1 (MQ=255)

                                                                           gggTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTg                >  1:2515403/1‑93 (MQ=255)

                                                                             gTTCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCa              <  1:5334458/93‑1 (MQ=255)

                                                                             gTTCGTTGTGAAAGCATACCCAGTCGAGGAATGCTCTTCAGAATTTGGGGCCGCCGTTGAGTAAAGTAAACACTTCTGGCGTTAACAACtacc              <  2:7823287/93‑4 (MQ=255)

                                                                              ttCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCAt             >  1:5382647/1‑93 (MQ=255)

                                                                               tCGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCAtt            >  1:2598612/1‑93 (MQ=255)

                                                                                cGTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTg           >  2:5417009/1‑93 (MQ=255)

                                                                                 gTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTgg          >  1:43171/1‑93 (MQ=255)

                                                                                 gTTGTGAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTgg          >  2:7093639/1‑93 (MQ=255)

                                                                                   tgtgAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCa        >  2:2383706/1‑93 (MQ=255)

                                                                                    gtgAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCAt       <  1:1414476/93‑1 (MQ=255)

                                                                                    gtgAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCAt       >  2:743575/1‑93 (MQ=255)

                                                                                    gtgAAAGCGTCCCCAGTCGAGGAATGCTCCTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCAt       <  2:688046/93‑1 (MQ=255)

                                                                                      gAAAGCGTCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCa     <  1:5940134/93‑1 (MQ=255)

ACGTGTCACCTGGCGCAGCGCCGCATTGCGCTGCTTGAGCAATCGCTTGAGATTGCTCCAGGCGGTGAAAAATCCGGGTTCGTTGTGAAAGCATCCCCAGTCGAGGAATGCTCTTCTGTATTTGGGGCCGCCGTTGAGTAAAGTAAACCCTTCTGGCGTTATCAACTGCATTGGCATCAGGT  >  NC_000913/3878340‑3878521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`