Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,929,523 T→C V194A (GTG→GCG)  kup → low affinity potassium transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,929,5230TC99.7% 1033.7 / NA 305V194A (GTG→GCG) kuplow affinity potassium transport system
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (126/178);  total (126/179)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 325 total aligned reads displayed.

TGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGT  >  NC_000913/3929433‑3929612
                                                                                          |                                                                                         
tGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAgcgc                                                                                         >  2:8494636/1‑93 (MQ=255)
 ggTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGcgct                                                                                        >  1:7043624/1‑93 (MQ=255)
  gTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGcgctg                                                                                       <  1:1577211/93‑1 (MQ=255)
     ggTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCAt                                                                                    >  1:8360349/1‑93 (MQ=255)
     ggTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCAt                                                                                    >  2:6669135/1‑93 (MQ=255)
     ggTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCAt                                                                                    >  1:536568/1‑93 (MQ=255)
      gcaaGCTGTTTGCGCCGATCACGCTGTCCTGGTTTTTGATTCTGGCCGGTTTTGTGTTAAGTAGCATTATTGCTTACTCGTAATTGCTGCATg                                                                                   >  2:1091588/3‑93 (MQ=255)
         aGCTGGTTGCGCCGATCATGCGGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATgcgc                                                                                <  1:2465308/93‑1 (MQ=255)
          gCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCt                                                                               >  1:6529966/1‑93 (MQ=255)
          gCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCt                                                                               >  2:4813765/1‑93 (MQ=255)
          gCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCt                                                                               <  1:2972422/93‑1 (MQ=255)
           cTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGTAAGCGCTGCATGCGCTg                                                                              <  1:4680879/93‑1 (MQ=255)
            tGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGa                                                                             >  1:2065729/1‑93 (MQ=255)
              tttGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAAt                                                                           >  2:1240094/1‑93 (MQ=255)
                 gcgcCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCa                                                                        >  1:6880757/1‑93 (MQ=255)
                    ccGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAAtg                                                                     >  2:2429037/1‑93 (MQ=255)
                     cGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAAtgt                                                                    <  2:7968948/93‑1 (MQ=255)
                      gATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAAtgtg                                                                   <  2:6190482/93‑1 (MQ=255)
                      gATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAAtgtg                                                                   >  1:6626480/1‑93 (MQ=255)
                       aTCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTgg                                                                  >  1:8226420/1‑93 (MQ=255)
                        tCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGGGCTGAATCCAATGTggg                                                                 >  1:2223750/1‑93 (MQ=255)
                        tCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTggg                                                                 >  2:5209323/1‑93 (MQ=255)
                        tCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTggg                                                                 <  2:2902125/93‑1 (MQ=255)
                          aTGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCg                                                               <  2:1585692/93‑1 (MQ=255)
                          aTGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCg                                                               >  1:4713816/1‑93 (MQ=255)
                           tGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCgg                                                              <  1:598231/93‑1 (MQ=255)
                            gCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGt                                                             >  1:7666635/1‑93 (MQ=255)
                            gCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGt                                                             >  1:5175582/1‑93 (MQ=255)
                              tGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGc                                                           >  1:2872911/1‑93 (MQ=255)
                               gACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCa                                                          >  1:4837740/1‑93 (MQ=255)
                               gACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCa                                                          >  1:1469733/1‑93 (MQ=255)
                                aCCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCAt                                                         >  1:4482782/1‑93 (MQ=255)
                                aCCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCAt                                                         <  1:3301964/93‑1 (MQ=255)
                                 ccTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCAtt                                                        >  1:6166077/1‑93 (MQ=255)
                                  cTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCAttt                                                       <  2:5337715/93‑1 (MQ=255)
                                     gTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATttctt                                                    >  1:167795/1‑93 (MQ=255)
                                      tttttGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATttcttc                                                   >  2:3590279/1‑93 (MQ=255)
                                      catttGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATttcttc                                                   >  2:2557085/3‑93 (MQ=255)
                                       ttttGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTcc                                                  >  1:5004135/1‑93 (MQ=255)
                                       ttttGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTcc                                                  <  1:302759/93‑1 (MQ=255)
                                        tttGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCt                                                 <  2:432454/93‑1 (MQ=255)
                                         ttGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCtt                                                <  1:574680/93‑1 (MQ=255)
                                          tGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTg                                               <  1:1853407/93‑1 (MQ=255)
                                          tGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTg                                               <  1:928589/93‑1 (MQ=255)
                                           taTTCTGGCAGGGCTGGGGTTACGTAGCATTATTGCTCACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTTCATTTCTTCCTTGa                                              <  1:2925513/92‑1 (MQ=255)
                                           gATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCCATGAGGGCCGTGCATTTCTTGCTTGa                                              >  1:2350431/1‑93 (MQ=255)
                                           gATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGa                                              >  2:3015213/1‑93 (MQ=255)
                                           gATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGa                                              <  2:6274827/93‑1 (MQ=255)
                                             ttCTGGCAGGACTGGGGTTACGTAGCATTATTGCTGACCCGGAAGCGCTGCATGTGCTGAATCCTAAGTGGGCGGCGCATTTCTTTCTTGAAt                                            >  2:8259340/1‑93 (MQ=255)
                                             ttCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAAt                                            >  2:5695923/1‑93 (MQ=255)
                                             ttCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAAt                                            <  1:6921006/93‑1 (MQ=255)
                                             ttCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAAt                                            >  1:3937710/1‑93 (MQ=255)
                                              tCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATa                                           >  2:864976/1‑93 (MQ=255)
                                                 ggCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACaa                                        >  1:2538297/1‑93 (MQ=255)
                                                    aGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAAc                                     <  1:585795/93‑1 (MQ=255)
                                                    aGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAAc                                     >  2:1934482/1‑93 (MQ=255)
                                                    aGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAAc                                     <  2:771053/93‑1 (MQ=255)
                                                    aGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAAc                                     <  1:3734424/93‑1 (MQ=255)
                                                     ggACTGGGGTTACGTGGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAACACg                                    >  2:3648347/1‑93 (MQ=255)
                                                     ggACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACg                                    <  2:2265909/93‑1 (MQ=255)
                                                     ggACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACg                                    >  1:3185346/1‑93 (MQ=255)
                                                      gACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACgg                                   <  1:86718/93‑1 (MQ=255)
                                                         tGGGGTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGttt                                <  2:4138972/93‑1 (MQ=255)
                                                           gggTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCt                              >  2:5972447/1‑93 (MQ=255)
                                                           gggTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCt                              <  1:3063438/93‑1 (MQ=255)
                                                             gTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCttt                            >  2:4454095/1‑93 (MQ=255)
                                                             gTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCttt                            <  2:7218100/93‑1 (MQ=255)
                                                             gTTACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCttt                            >  2:6387551/1‑93 (MQ=255)
                                                              ttACGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCtttt                           >  1:3529296/1‑93 (MQ=255)
                                                                 cGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTAtt                        >  2:4609828/1‑93 (MQ=255)
                                                                 cGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTAtt                        <  1:1645417/93‑1 (MQ=255)
                                                                 cGTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTAtt                        >  1:7538456/1‑93 (MQ=255)
                                                                  gTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTg                       >  1:3562293/1‑93 (MQ=255)
                                                                  gTAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTg                       >  1:5269346/1‑93 (MQ=255)
                                                                   tAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGc                      <  1:1262336/93‑1 (MQ=255)
                                                                   tAGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGc                      <  1:5862028/93‑1 (MQ=255)
                                                                    aGCATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCa                     <  2:5123535/93‑1 (MQ=255)
                                                                      cATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCAtt                   <  2:4787612/93‑1 (MQ=255)
                                                                      cATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCAtt                   <  2:5268978/93‑1 (MQ=255)
                                                                      cATTATTGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCAtt                   >  2:7303899/1‑93 (MQ=255)
                                                                        ttattGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAg                 >  2:3888720/1‑93 (MQ=255)
                                                                         tattGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAgg                <  2:493150/93‑1 (MQ=255)
                                                                           ttGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAgggg              >  1:4379679/1‑93 (MQ=255)
                                                                            tGCTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGc             <  2:2024140/93‑1 (MQ=255)
                                                                              cTAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAg           <  1:1027585/93‑1 (MQ=255)
                                                                               tAACCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAgt          <  1:2788060/93‑1 (MQ=255)
                                                                               tAACCCGGAAGCGCTGCATGCGCCGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAgt          >  2:5728563/1‑93 (MQ=255)
                                                                               cagcccGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAgt          <  1:6205378/90‑1 (MQ=255)
                                                                                aaCCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAgtg         <  2:8613626/93‑1 (MQ=255)
                                                                                aaCCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGGGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAgtg         >  2:3176142/1‑93 (MQ=255)
                                                                                 aCCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAgtgg        >  2:5361136/1‑93 (MQ=255)
                                                                                 aCCCGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAgtgg        >  2:6813643/1‑93 (MQ=255)
                                                                                  cccGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAgtggt       <  1:559762/93‑1 (MQ=255)
                                                                                  cccGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAgtggt       >  2:3282961/1‑93 (MQ=255)
                                                                                  cccGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAgtggt       <  1:3787154/93‑1 (MQ=255)
                                                                                   ccGGAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAgtggtg      >  2:3525335/1‑93 (MQ=255)
                                                                                     ggAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGtgtt    >  1:73060/1‑93 (MQ=255)
                                                                                     ggAAGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGtgtt    <  1:4225808/93‑1 (MQ=255)
                                                                                     ggAAGCCCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGtgtt    <  2:214777/93‑1 (MQ=255)
                                                                                       aaGCGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGtgttgt  >  1:79535/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGT  >  NC_000913/3929433‑3929612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑