BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	518,231	T→C	K48R (AAA→AGA)	ybbO ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	518,231	0	T	C	99.7%	1339.3 / ‑4.9	393	K48R (AAA→AGA)	ybbO	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (192/199); total (193/200)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.78e-01

Only 100 of 417 total aligned reads displayed.

TCGGCTGCGCGATCAACACTTTCTGGTGAATCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTTTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCAATTCCACTGGAACATCCGGTAATTAAGA  >  NC_000913/518141‑518321

tcgGCTGCGCGATCAACACTTTCTGGTGAATCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGtctc                                                                                          >  2:6446217/1‑93 (MQ=255)

   gCTGCGCGATCAACACTTTCTGGTGAATCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCgg                                                                                       >  1:7982794/1‑93 (MQ=255)

        gcgATCAACACTTTCTGGTGAATCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAAcc                                                                                  <  1:5126311/93‑1 (MQ=255)

         cgATCAACACTTTCTGGTGAATCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCt                                                                                 >  1:448693/1‑93 (MQ=255)

           aTCAACACTTTCTGGTGAATCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGc                                                                               <  1:8295550/93‑1 (MQ=255)

           aTCAACACTTTCTGGTGAATCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGc                                                                               <  1:4252043/93‑1 (MQ=255)

           aTCAACACTTTCTGGTGAATCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGc                                                                               >  2:2990323/1‑93 (MQ=255)

            tCAACACTTTCTGGTGAATCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGcc                                                                              <  1:1542214/93‑1 (MQ=255)

            tCAACACTTTCTGGTGAATCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGcc                                                                              <  1:952433/93‑1 (MQ=255)

             cAACACTTTCTGGTGAATCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCa                                                                             >  1:7112074/1‑93 (MQ=255)

              aaCACTTTCTGGTGAATCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAg                                                                            >  2:7516066/1‑93 (MQ=255)

               acacTTTCTGGTGAATCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGc                                                                           <  2:4935651/93‑1 (MQ=255)

                cacTTTCTGGTGAATCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGca                                                                          <  2:811495/93‑1 (MQ=255)

                cacTTTCTGGTGAATCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGca                                                                          <  1:4789403/93‑1 (MQ=255)

                  cTTTCTGGTGAATCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGcaca                                                                        >  1:8792596/1‑93 (MQ=255)

                  cTTTCTGGTGAATCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGcaca                                                                        >  1:4602947/1‑93 (MQ=255)

                  cTTTCTGGTGAATCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGcaca                                                                        >  1:2701409/1‑93 (MQ=255)

                    ttCTGGTGAATCCAGATCGATCCACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATg                                                                      <  2:4408907/93‑1 (MQ=255)

                    ttCTGGTGAATCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATg                                                                      <  1:5987498/93‑1 (MQ=255)

                     tCTGGTGAATCTAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGa                                                                     >  1:4655079/1‑93 (MQ=255)

                     tCTGGTGAATCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGa                                                                     >  1:3216034/1‑93 (MQ=255)

                         gTGAATCCAGATCGATCACCACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAAc                                                                 <  2:1596899/93‑1 (MQ=255)

                         gTGAATCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAAc                                                                 <  2:3102592/93‑1 (MQ=255)

                           gAATCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAAccc                                                               >  1:4326133/1‑93 (MQ=255)

                           gAATCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAAccc                                                               >  1:3302368/1‑93 (MQ=255)

                           gAATCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAAccc                                                               <  1:7874898/93‑1 (MQ=255)

                            aaTCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCt                                                              >  2:5712142/1‑93 (MQ=255)

                            aaTCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCt                                                              >  1:6360734/1‑93 (MQ=255)

                             aTCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTg                                                             >  1:7198234/1‑93 (MQ=255)

                             aTCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGACTCCGGCAACCTGCCAGCACATGAAAACCCTg                                                             <  1:7818570/93‑1 (MQ=255)

                              tCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTgg                                                            <  1:1212421/93‑1 (MQ=255)

                               ccAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTggg                                                           >  1:3544687/1‑92 (MQ=255)

                               ccAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGc                                                           <  1:1666862/93‑1 (MQ=255)

                                 aGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGt                                                         >  1:1164789/1‑93 (MQ=255)

                                 aGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACAACATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGt                                                         >  1:5362520/1‑93 (MQ=255)

                                  gatcgatcAACCCGCCGGTAAATCCCATGCTGTTCATCCGCTCAACATCATCCGGTGTCCGGCAACCTGCCAACACAGACAAACCCTGGCCtt                                                        >  2:4464332/1‑93 (MQ=255)

                                    tcgatcAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGtttt                                                      <  1:5813911/93‑1 (MQ=255)

                                     cgatcAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTa                                                     >  2:4048840/1‑93 (MQ=255)

                                     cgatcAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTa                                                     >  2:3411575/1‑93 (MQ=255)

                                       atcAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAAt                                                   >  2:1090332/1‑93 (MQ=255)

                                        tcAACACGCCGGTAAATCCCATGCTGTTCATTCGCTTAATATCATCCGGTCTCCGGCAACCTGCTAGCACATGAAAACCCTGGTGTTTTAAtt                                                  >  2:8088553/1‑93 (MQ=255)

                                        tcAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAAtt                                                  >  1:1251920/1‑93 (MQ=255)

                                        tcAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAAtt                                                  <  2:1291344/93‑1 (MQ=255)

                                        tcAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAAtt                                                  >  1:5047536/1‑93 (MQ=255)

                                         cAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTc                                                 <  2:8803036/93‑1 (MQ=255)

                                         cAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTc                                                 <  2:5099918/93‑1 (MQ=255)

                                          aaCACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCg                                                <  2:1763686/93‑1 (MQ=255)

                                           acacGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGa                                               >  2:1462801/1‑93 (MQ=255)

                                             ccGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAgc                                             <  2:7115341/92‑1 (MQ=255)

                                             acGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAgc                                             >  1:2249703/1‑93 (MQ=255)

                                             acGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAgc                                             <  1:2601858/93‑1 (MQ=255)

                                             acGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAgc                                             <  1:3861800/93‑1 (MQ=255)

                                              cGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAgcg                                            <  1:5560672/93‑1 (MQ=255)

                                              cGCCGATAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAgcg                                            <  2:5401920/93‑1 (MQ=255)

                                                 cGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAgcgcgc                                         >  1:1905307/1‑93 (MQ=255)

                                                 cGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAgcgcgc                                         >  1:6960269/1‑93 (MQ=255)

                                                  ggTCAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCt                                        <  2:3285525/93‑1 (MQ=255)

                                                  ggTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCt                                        >  2:5644147/1‑93 (MQ=255)

                                                  ggTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCt                                        >  2:730329/1‑93 (MQ=255)

                                                    tAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCttt                                      >  2:6733005/1‑93 (MQ=255)

                                                     aaaTCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTc                                     <  2:6843600/93‑1 (MQ=255)

                                                       aacccATGCTGTTCATGCCCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCa                                   <  1:1040052/91‑1 (MQ=255)

                                                       aTCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCa                                   <  1:6736144/93‑1 (MQ=255)

                                                        tCCCATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAg                                  >  1:859265/1‑93 (MQ=255)

                                                           cATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGcc                               >  1:4141924/1‑93 (MQ=255)

                                                           cATGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGcc                               >  1:6595120/1‑93 (MQ=255)

                                                             tGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCaa                             <  1:5736021/93‑1 (MQ=255)

                                                             tGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCaa                             <  2:8727155/93‑1 (MQ=255)

                                                             tGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCaa                             <  1:4367371/93‑1 (MQ=255)

                                                             tGCTGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCaa                             >  2:8355803/1‑93 (MQ=255)

                                                             tGCTATTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAGCCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCaa                             <  1:6690233/93‑1 (MQ=255)

                                                                tGTTCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCAATTc                          >  1:2530618/1‑93 (MQ=255)

                                                                   tCATGCGCTCAACATCATCCGGTCTCCGGCACCCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGGCAATTCCAc                       <  2:7954425/93‑1 (MQ=255)

                                                                   tCATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCAATTCCAc                       <  1:5421031/93‑1 (MQ=255)

                                                                    cATGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCAATTCCACt                      <  2:6063967/93‑1 (MQ=255)

                                                                      tGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCAATTCCACTgg                    >  2:2697567/1‑93 (MQ=255)

                                                                      tGCGCTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCAATTCCACTgg                    >  2:761399/1‑93 (MQ=255)

                                                                         gcTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCAATTCCACTGGAAc                 <  1:6244445/93‑1 (MQ=255)

                                                                          cTCAACATCATCCGGTCTCCGGCTACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCAATTCCACTGGAACa                <  2:4757247/93‑1 (MQ=255)

                                                                          cTCAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCAATTCCACTGGAACa                >  2:2188703/1‑93 (MQ=255)

                                                                            cAACATCATCCGGTCTCCGGCAACCTGCCAGCACATGAAAGCCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCAATTCCACTGGAACATc              <  1:2965046/93‑1 (MQ=255)

                                                                              aCATCATCCGGTCTCCGCCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCAATTCCACTGGAACATCCg            <  2:5792881/93‑1 (MQ=255)

                                                                                atcatcCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCAATTCCACTGGAACATCCGGt          >  2:4220141/1‑93 (MQ=255)

                                                                                atcatcCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCAATTCCACTGGAACATCCGGt          >  2:1248444/1‑93 (MQ=255)

                                                                                 tcatcCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCAATTCCACTGGAACATCCGGTa         >  2:5938116/1‑93 (MQ=255)

                                                                                 tcatcCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCAATTCCACTGGAACATCCGGTa         >  1:7452395/1‑93 (MQ=255)

                                                                                  catcCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCAATTCCACTGGAACATCCGGTaa        <  2:459423/93‑1 (MQ=255)

                                                                                   atcCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCAATTCCACTGGAACATCCGGTAAt       <  2:4711906/93‑1 (MQ=255)

                                                                                   atcCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCAATTCCACTGGAACATCCGGTAAt       >  2:2400354/1‑93 (MQ=255)

                                                                                   atcCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCAATTCCACTGGAACATCCGGTAAt       <  2:2025933/93‑1 (MQ=255)

                                                                                    tcCGGTCTCCGGCAACCTGCCAGCACATGAAATCCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCAATTCCACTGGAACATCCGGTAAtt      <  1:7733184/93‑1 (MQ=255)

                                                                                    tcCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCAATTCCACTGGAACATCCGGTAAtt      >  1:5081482/1‑93 (MQ=255)

                                                                                    tcCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCAATTCCACTGGAACATCCGGTAAtt      <  2:5515033/93‑1 (MQ=255)

                                                                                    tcCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCAATTCCACTGGAACATCCGGTAAtt      <  1:5897936/93‑1 (MQ=255)

                                                                                    tcCGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCAATTCCACTGGAACATCCGGTAAtt      >  2:5272470/1‑93 (MQ=255)

                                                                                      cGGTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCAATTCCACTGGAACATCCGGTAATTaa    <  2:6988482/93‑1 (MQ=255)

                                                                                       ggTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCCGGCCAATTCCACTGGAACATCCGGTAATTAAg   <  2:655588/93‑1 (MQ=255)

                                                                                       ggTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCAATTCCACTGGAACATCCGGTAATTAAg   >  2:7196193/1‑93 (MQ=255)

                                                                                       ggTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCAATTCCACTGGAACATCCGGTAATTAAg   <  2:3753896/93‑1 (MQ=255)

                                                                                       ggTCTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCAATTCCACTGGAACATCCGGTAATTAAg   <  1:7595112/93‑1 (MQ=255)

TCGGCTGCGCGATCAACACTTTCTGGTGAATCCAGATCGATCAACACGCCGGTAAATCCCATGCTGTTCATGCGCTCAACATCATCCGGTTTCCGGCAACCTGCCAGCACATGAAAACCCTGGCGTTTTAATTCGAGCGCGCTTTCCAGGCCAATTCCACTGGAACATCCGGTAATTAAGA  >  NC_000913/518141‑518321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`