Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 531,406 C→T A14T (GCT→ACT)  ybbS ← putative transcriptional regulator LYSR‑type

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913531,4060CT99.7% 1314.7 / NA 371A14T (GCT→ACT) ybbSputative transcriptional regulator LYSR‑type
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (190/179);  minor base G (0/1);  total (191/180)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 381 total aligned reads displayed.

TATTCTCTTCCAGAAGTTTAATGCGATAACTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGCAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTC  >  NC_000913/531316‑531495
                                                                                          |                                                                                         
   tctcTTCCAGAAGTTTAATGCGATAACTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCg                                                                                      >  1:690720/1‑93 (MQ=255)
     tctTCCAGAAGTTTAATGCGATAACTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCa                                                                                    >  1:8378446/1‑93 (MQ=255)
      ctTCCAGAAGTTTAATGCGATAACTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCaa                                                                                   <  1:6269687/93‑1 (MQ=255)
      ctTCCAGAAGTTTAATGCGATAACTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCaa                                                                                   >  1:4580981/1‑93 (MQ=255)
         ccAGAAGTTTAATGCGATAACTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGa                                                                                >  1:5532255/1‑93 (MQ=255)
         ccAGAAGTTTAATGCGATAACTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGa                                                                                >  1:3229713/1‑93 (MQ=255)
          cAGAAGTTTAATGCGATAACTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAGCTTCCTGTTTCAGTAACCGCAATGaa                                                                               <  2:633793/93‑1 (MQ=255)
          cAGAAGTTTAATGCGATAACTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGaa                                                                               >  1:4296978/1‑93 (MQ=255)
          cAGAAGTTTAATGCGATAACTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGaa                                                                               >  2:1159757/1‑93 (MQ=255)
           aGAAGTTTAATGCGATAACTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGaaa                                                                              <  2:567091/93‑1 (MQ=255)
            gAAGTTTAATGCGATAACTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAg                                                                             >  2:4572336/1‑93 (MQ=255)
             aaGTTTAATGCGATAACTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTACCCGCAATGAAAgc                                                                            >  1:7136440/1‑92 (MQ=255)
             aaGTTTAATGCGATAACTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGt                                                                            >  2:5402275/1‑93 (MQ=255)
              aGTTTAATGCGATAACTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTc                                                                           <  2:4017520/93‑1 (MQ=255)
              aGTTTAATGCGATAACTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTc                                                                           >  1:594279/1‑93 (MQ=255)
              aGTTTAATGCGATAACTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTc                                                                           <  1:8194899/93‑1 (MQ=255)
              aGTTTAATGCGATAACTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTc                                                                           <  2:3126006/93‑1 (MQ=255)
                tttAATGCGATAACTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCg                                                                         >  1:5250891/1‑93 (MQ=255)
                tttAATGCGATAACTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGCAACCGCAATGAAAGTCCg                                                                         >  1:6051401/1‑93 (MQ=255)
                   aaTGCGATAACTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCaa                                                                      <  2:7939264/93‑1 (MQ=255)
                    aTGCGATAACTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAg                                                                     <  1:6992162/93‑1 (MQ=255)
                       cGATAACTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGtt                                                                  >  2:6854054/1‑93 (MQ=255)
                        gATAACTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGttt                                                                 >  1:6662868/1‑93 (MQ=255)
                        gATAACTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGttt                                                                 <  2:5095437/93‑1 (MQ=255)
                         aTAACTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCGGTAACCGCAATGAAAGTCCGCAAGGTTTc                                                                >  1:2770996/1‑93 (MQ=255)
                         aTAACTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTc                                                                <  2:5319760/93‑1 (MQ=255)
                         aTAACTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTc                                                                >  2:189363/1‑93 (MQ=255)
                          tAACTCGTCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAATCTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCt                                                               <  2:1586998/93‑1 (MQ=255)
                           aaCTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTg                                                              >  1:806831/1‑93 (MQ=255)
                               gATCGTCGCCGTAGTGTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGGAACCGCAATGAAAGTCCGCAAGGTTTCTGGATc                                                          <  1:2975495/93‑1 (MQ=255)
                                  cgtcgCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGaa                                                       <  1:2407537/93‑1 (MQ=255)
                                      gCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATc                                                   <  1:7274069/93‑1 (MQ=255)
                                       ccGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCt                                                  >  2:2924428/1‑93 (MQ=255)
                                       ccGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCt                                                  >  1:1995741/1‑93 (MQ=255)
                                       ccGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCt                                                  >  1:2088610/1‑93 (MQ=255)
                                        cGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCtt                                                 <  2:8283364/93‑1 (MQ=255)
                                        cGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCtt                                                 <  2:8176298/93‑1 (MQ=255)
                                        cGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCtt                                                 <  2:3426582/93‑1 (MQ=255)
                                         gTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTc                                                >  1:7673489/1‑93 (MQ=255)
                                          tGGTTTTACATAATCGTTCTGCCGCTTTTCAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCa                                               <  2:7087823/93‑1 (MQ=255)
                                           ggTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAg                                              <  1:1572043/93‑1 (MQ=255)
                                             ttttACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGt                                            <  2:7669563/93‑1 (MQ=255)
                                              tttACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTa                                           >  2:2056197/1‑93 (MQ=255)
                                              tttACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTa                                           >  2:3197194/1‑93 (MQ=255)
                                                tACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGACCATCTTCAGGTATc                                         >  1:3827498/1‑93 (MQ=255)
                                                 aCATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATcc                                        <  2:4839005/93‑1 (MQ=255)
                                                   aTAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATcccc                                      >  2:8013969/1‑93 (MQ=255)
                                                   aTAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATcccc                                      <  2:3730898/93‑1 (MQ=255)
                                                   aTAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATcccc                                      <  2:255502/93‑1 (MQ=255)
                                                    tAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCt                                     <  2:3488697/93‑1 (MQ=255)
                                                    tAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCt                                     >  2:4726813/1‑93 (MQ=255)
                                                    tAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCt                                     >  2:2119409/1‑93 (MQ=255)
                                                    tAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCt                                     >  2:1718548/1‑93 (MQ=255)
                                                     aaTCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCtt                                    >  1:172437/1‑93 (MQ=255)
                                                     aaTCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCtt                                    <  1:7479467/93‑1 (MQ=255)
                                                      aTCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCttt                                   >  1:3149420/1‑93 (MQ=255)
                                                          ttCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTaaa                               >  2:235956/1‑93 (MQ=255)
                                                          ttCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTaaa                               <  1:3898785/93‑1 (MQ=255)
                                                          ttCTGCCGCTTTTGAAAAACTTCCCGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTaaa                               >  2:6062241/1‑93 (MQ=255)
                                                           tCTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAAt                              <  2:661065/93‑1 (MQ=255)
                                                            cTGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATc                             >  1:4949683/1‑93 (MQ=255)
                                                             tGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATcc                            >  2:2445094/1‑93 (MQ=255)
                                                             tGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATcc                            <  1:1891280/93‑1 (MQ=255)
                                                             tGCCGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATcc                            >  2:6407942/1‑93 (MQ=255)
                                                                cGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCa                         <  1:5386579/93‑1 (MQ=255)
                                                                cGCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCa                         >  2:2704010/1‑93 (MQ=255)
                                                                 gCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCaa                        <  1:1181486/93‑1 (MQ=255)
                                                                 gCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCaa                        <  2:694010/93‑1 (MQ=255)
                                                                 gCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCaa                        >  2:3570400/1‑93 (MQ=255)
                                                                 gCTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCaa                        <  1:7868160/93‑1 (MQ=255)
                                                                  cTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAg                       >  2:6683353/1‑93 (MQ=255)
                                                                  cTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAg                       <  2:5511241/93‑1 (MQ=255)
                                                                  cTTTTGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGGTTCTGGATCGAACATCTTTAGGTATCCCCCTTTTAATACGCAAg                       >  2:8799507/1‑93 (MQ=255)
                                                                   ttttGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGt                      >  2:7212706/1‑93 (MQ=255)
                                                                   ttttGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGt                      >  2:6007216/1‑93 (MQ=255)
                                                                   ttttGAAAAACCTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGt                      >  2:2815998/1‑93 (MQ=255)
                                                                     ttGAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTg                    >  2:2497052/1‑93 (MQ=255)
                                                                       gAAAAACTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCg                  >  1:1062193/1‑93 (MQ=255)
                                                                        aaaaaCTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGt                 <  2:8609470/93‑1 (MQ=255)
                                                                        aaaaaCTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGt                 <  1:6093902/93‑1 (MQ=255)
                                                                        aaaaaCTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGt                 >  2:490473/1‑93 (MQ=255)
                                                                         aaaaCTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTg                <  1:3342933/93‑1 (MQ=255)
                                                                         aaaaCTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTg                >  1:6452665/1‑93 (MQ=255)
                                                                          aaaCTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGa               >  1:4245934/1‑93 (MQ=255)
                                                                           aaCTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGAt              >  2:2978424/1‑93 (MQ=255)
                                                                           aaCTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGAt              <  2:6194826/93‑1 (MQ=255)
                                                                            aCTTCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGAtt             <  2:458626/93‑1 (MQ=255)
                                                                             cTTCCTGTTTCAGTAACCGCAATCAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGAttt            >  2:4939776/1‑93 (MQ=255)
                                                                              ttCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGAtttt           >  1:8619564/1‑93 (MQ=255)
                                                                              ttCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGAtttt           >  1:8664269/1‑93 (MQ=255)
                                                                              ttCCTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGAtttt           >  2:7365887/1‑93 (MQ=255)
                                                                                ccTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCt         >  2:3372718/1‑93 (MQ=255)
                                                                                 cTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCTCCTTTTAAATCAGCAAGTTGCGTGATTTTCtt        >  2:4289347/1‑93 (MQ=255)
                                                                                 cTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCtt        <  1:5054752/93‑1 (MQ=255)
                                                                                 cTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCtt        <  1:3675635/93‑1 (MQ=255)
                                                                                 cTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCtt        >  1:1262641/1‑93 (MQ=255)
                                                                                 cTGTTTCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCtt        <  2:5179690/93‑1 (MQ=255)
                                                                                    tttCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATc     <  2:1456455/93‑1 (MQ=255)
                                                                                    tttCAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAAGCCGCAAGTTGCGTGATTTTCTTATc     <  1:851045/93‑1 (MQ=255)
                                                                                       cAGTAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCctc  >  1:8752073/1‑93 (MQ=255)
                                                                                          |                                                                                         
TATTCTCTTCCAGAAGTTTAATGCGATAACTGATCGTCGCCGTGGTTTTACATAATCGTTCTGCCGCTTTTGAAAAACTTCCTGTTTCAGCAACCGCAATGAAAGTCCGCAAGGTTTCTGGATCGAACATCTTCAGGTATCCCCTTTTAAATCCGCAAGTTGCGTGATTTTCTTATCCTC  >  NC_000913/531316‑531495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑