BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	542,052	A→G	Q314R (CAG→CGG)	ybbZ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	542,052	0	A	G	99.6%	930.9 / ‑4.8	271	Q314R (CAG→CGG)	ybbZ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (152/118); total (152/119)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.17e-01

Only 100 of 286 total aligned reads displayed.

GCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTACGGCATTGACGCGGTATTCAGCA  >  NC_000913/541962‑542141

 cgcAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGTCTGGGGTAAAGCGCCGCTCGGTGTTGTGTCGGTGGCTATGCGGtt                                                                                        >  2:4488870/1‑93 (MQ=255)

 cgcAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAGtt                                                                                        <  2:8482431/93‑1 (MQ=255)

 agcagcaCTGGTGATTACCGGGGAAGGCCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGtt                                                                                        <  2:62816/92‑1 (MQ=255)

  gcagcaCTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGttt                                                                                       >  2:1002294/1‑93 (MQ=255)

    agcaCTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTaa                                                                                     <  1:8194341/93‑1 (MQ=255)

     gcaCTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAAt                                                                                    >  1:5019118/1‑93 (MQ=255)

     gcaCTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAAt                                                                                    >  2:7862430/1‑93 (MQ=255)

      caCTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATg                                                                                   <  2:1149415/93‑1 (MQ=255)

       aCTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGt                                                                                  <  2:3153141/93‑1 (MQ=255)

        cTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTa                                                                                 >  2:6378024/1‑93 (MQ=255)

         tGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTAc                                                                                >  1:7677473/1‑93 (MQ=255)

          ggTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTAcc                                                                               >  1:3138848/1‑93 (MQ=255)

          ggTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTAcc                                                                               <  1:3649205/93‑1 (MQ=255)

            tGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCgg                                                                             <  2:8060045/93‑1 (MQ=255)

             gATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGt                                                                            <  2:2592051/93‑1 (MQ=255)

              aTTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTg                                                                           <  2:860839/93‑1 (MQ=255)

               ttACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGa                                                                          <  1:7550656/93‑1 (MQ=255)

                tACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGAt                                                                         >  1:257821/1‑93 (MQ=255)

                  ccGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTg                                                                       <  2:3180941/93‑1 (MQ=255)

                    ggggAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGTGGTTTAATGTACCGGTGATTggg                                                                     >  2:6264462/1‑93 (MQ=255)

                    ggggAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTggg                                                                     >  1:6396202/1‑93 (MQ=255)

                       gAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGAtt                                                                  <  2:1538235/93‑1 (MQ=255)

                       gAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGAtt                                                                  >  1:5745525/1‑93 (MQ=255)

                        aaGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTg                                                                 <  2:4388991/93‑1 (MQ=255)

                         aGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGc                                                                >  2:2142031/1‑93 (MQ=255)

                         aGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGc                                                                <  1:4406615/93‑1 (MQ=255)

                         aGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGc                                                                <  2:3702248/93‑1 (MQ=255)

                         aGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGc                                                                <  2:6630536/93‑1 (MQ=255)

                          gggCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCt                                                               <  1:3523902/93‑1 (MQ=255)

                          gggCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCt                                                               >  2:8549135/1‑93 (MQ=255)

                            gcgcATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTgg                                                             >  1:1256623/1‑93 (MQ=255)

                              gcATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCg                                                           >  1:4519818/1‑93 (MQ=255)

                              gcATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCg                                                           >  1:5931929/1‑93 (MQ=255)

                               cATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGt                                                          >  1:5209691/1‑93 (MQ=255)

                                 tCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAGTTTAATGTACCGGTGATTGGGATTGCTGGCGTAt                                                        >  1:8047782/1‑93 (MQ=255)

                                  cGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTAtt                                                       >  2:7502646/1‑93 (MQ=255)

                                   gACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTg                                                      >  2:6957416/1‑93 (MQ=255)

                                   gACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGGGATTGGGATTGCTGGCGTATTg                                                      >  2:2103161/1‑93 (MQ=255)

                                    aCTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTgg                                                     <  1:1571635/93‑1 (MQ=255)

                                     cTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTggg                                                    >  2:882050/1‑93 (MQ=255)

                                     cTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTggg                                                    >  1:6630284/1‑93 (MQ=255)

                                      tCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGt                                                   >  1:3792771/1‑93 (MQ=255)

                                          aaaCGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATg                                               <  1:6171356/93‑1 (MQ=255)

                                           aaCGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATgg                                              <  1:2238959/93‑1 (MQ=255)

                                            aCGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGc                                             <  1:1471872/93‑1 (MQ=255)

                                            aCGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGc                                             <  1:2965272/93‑1 (MQ=255)

                                              ggCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGt                                           <  1:7717297/93‑1 (MQ=255)

                                              ggCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGt                                           <  1:8507736/93‑1 (MQ=255)

                                              ggCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGt                                           <  1:7500643/93‑1 (MQ=255)

                                              ggCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGt                                           >  2:701925/1‑93 (MQ=255)

                                               gCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTg                                          >  1:5954000/1‑93 (MQ=255)

                                                 aGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGa                                        >  2:1269577/1‑93 (MQ=255)

                                                  ggCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGaa                                       <  1:7896647/93‑1 (MQ=255)

                                                   gCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAg                                      <  1:7254640/93‑1 (MQ=255)

                                                    gggTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAgt                                     <  2:1913434/92‑1 (MQ=255)

                                                    cGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTCCCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAgt                                     <  2:5440757/93‑1 (MQ=255)

                                                     ggTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAgtg                                    >  1:52854/1‑93 (MQ=255)

                                                     ggTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAgtg                                    <  2:8032424/93‑1 (MQ=255)

                                                      gTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAgtgg                                   >  2:3925218/1‑93 (MQ=255)

                                                      gTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAgtgg                                   >  1:5099981/1‑93 (MQ=255)

                                                      gTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAgtgg                                   >  1:1398968/1‑93 (MQ=255)

                                                       tAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAgtggt                                  <  2:4024151/93‑1 (MQ=255)

                                                       tAAAGCGCCGCTGGCTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAgtggt                                  <  1:6068190/93‑1 (MQ=255)

                                                        aaaGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAgtggtg                                 >  2:592441/1‑93 (MQ=255)

                                                           gcgcCGCTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCAc                              >  1:4328345/1‑93 (MQ=255)

                                                            cgccgcTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCAcc                             >  1:4036349/1‑93 (MQ=255)

                                                             gccgcTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCa                            >  1:1814065/1‑93 (MQ=255)

                                                             gccgcTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCa                            >  2:1796659/1‑93 (MQ=255)

                                                                gcTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTa                         <  2:8347137/93‑1 (MQ=255)

                                                                gcTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTa                         >  1:6772107/1‑93 (MQ=255)

                                                                 cTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTAc                        >  2:2037797/1‑93 (MQ=255)

                                                                 cTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTAc                        <  1:4834498/93‑1 (MQ=255)

                                                                 cTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTAc                        <  2:5076397/93‑1 (MQ=255)

                                                                 cTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTAc                        >  2:7213336/1‑93 (MQ=255)

                                                                 cTGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTAc                        >  1:6541840/1‑93 (MQ=255)

                                                                  tGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTACg                       >  2:5847078/1‑93 (MQ=255)

                                                                  tGGGTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTACg                       >  2:8012909/1‑93 (MQ=255)

                                                                   gggTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTACgg                      >  2:4584569/1‑93 (MQ=255)

                                                                    ggTGTGGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTACGGc                     <  1:3677771/93‑1 (MQ=255)

                                                                       gtgGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTACGGCAtt                  <  1:3287443/93‑1 (MQ=255)

                                                                       gtgGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTACGGCAtt                  >  2:7779971/1‑93 (MQ=255)

                                                                        tgGCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTACGGCATTg                 <  1:741938/93‑1 (MQ=255)

                                                                        gggCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTACGGCATTg                 <  2:7339764/92‑1 (MQ=255)

                                                                         ggCGTCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTACGGCATTGa                <  2:5800807/93‑1 (MQ=255)

                                                                          gcggcgGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTACGGCATTGAc               <  1:5594762/93‑1 (MQ=255)

                                                                             tCGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTACGGCATTGAcgcg            <  2:2741347/93‑1 (MQ=255)

                                                                              cGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTACGGCATTGACGCgg           >  1:101916/1‑93 (MQ=255)

                                                                              cGGTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTACGGCATTGACGCgg           <  2:233917/93‑1 (MQ=255)

                                                                               ggTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTACGGCATTGACGCGGt          >  1:2200285/1‑93 (MQ=255)

                                                                                gTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTACGGCATTGACGCggat         >  2:3020650/1‑91 (MQ=255)

                                                                                gTGGCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTACGGCATTGACGCGGTa         >  1:7057585/1‑93 (MQ=255)

                                                                                  ggCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTACGGCATTGACGCGGTAtt       >  2:397518/1‑93 (MQ=255)

                                                                                  ggCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTACGGCATTGACGCGGTAtt       >  1:8801479/1‑93 (MQ=255)

                                                                                   gCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTACGGCATTGACGCGGTATTc      <  2:4244128/93‑1 (MQ=255)

                                                                                   gCGAAGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTACGGCATTGACGCGGTATTc      >  2:1275887/1‑93 (MQ=255)

                                                                                     gAAGCGGTTTAATGTACCGGGGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGCACGGCATTGACGCGGCATTCAg    >  2:6735289/1‑93 (MQ=255)

                                                                                      aaGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTACGGCATTGACGCGGTATTCAGc   >  1:2712609/1‑93 (MQ=255)

                                                                                      aaGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTACGGCATTGACGCGGTATTCAGc   >  2:1670368/1‑93 (MQ=255)

                                                                                       aGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTACGGCATTGACGCGGTATTCAGct  >  1:5919462/1‑92 (MQ=255)

                                                                                       aGCGGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTACGGCATTGACGCGGTATTCAGCa  >  2:4932202/1‑93 (MQ=255)

GCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGCTGGGTGTGGCGTCGGTGGCGAAGCAGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTACGGCATTGACGCGGTATTCAGCA  >  NC_000913/541962‑542141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`