BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	547,694	A→G	E38E (GAA→GAG)	b4507 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	547,694	0	A	G	99.7%	1055.4 / NA	305	E38E (GAA→GAG)	b4507	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (136/167); total (137/168)
Fisher's exact test for biased strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 326 total aligned reads displayed.

CAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTT  >  NC_000913/547604‑547783

 aaTGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGc                                                                                         <  1:6440896/93‑1 (MQ=255)

 aaTGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGc                                                                                         <  1:7668099/93‑1 (MQ=255)

  aTGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCa                                                                                        >  1:232810/1‑93 (MQ=255)

   tgcttcCGTTATCCAACAAAATCGTCGCGCTCCTCCACACTTGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaa                                                                                       <  1:2937297/93‑1 (MQ=255)

   tgctgcGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaa                                                                                       <  1:1731058/93‑1 (MQ=255)

    gctgcGGTTATCGAACAAATTCGGCGCGCTCGTCCACCTTGGCTGAATGTCCAACCGTCTTCTTCACTTATAAGCGAACTAAACGAGGGaaa‑a                                                                                     <  1:1498884/93‑1 (MQ=255)

     ctgcGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaa‑aa                                                                                    >  1:3742468/1‑93 (MQ=255)

      tgcGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑Aac                                                                                   <  1:1863688/93‑1 (MQ=255)

       gcGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑Aaca                                                                                  <  1:2842886/93‑1 (MQ=255)

        cGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑Aacac                                                                                 <  2:4430995/93‑1 (MQ=255)

          gTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCCCTTATCAGCGAACTAAACGCGGGCAA‑AACACTg                                                                               >  1:2966289/1‑93 (MQ=255)

            tATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCt                                                                             >  1:8197831/1‑93 (MQ=255)

              tCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTc                                                                           <  2:2057005/93‑1 (MQ=255)

                gAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACTGGCTTCTTCACTTATAAGCGAACTAAACGAGGGCAA‑AACACTGCTTCAc                                                                         >  2:1931798/1‑93 (MQ=255)

                gAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCAc                                                                         >  1:2414357/1‑93 (MQ=255)

                 aaCAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACg                                                                        >  2:2812383/1‑93 (MQ=255)

                  aCAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGc                                                                       <  1:8070211/93‑1 (MQ=255)

                  aCAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGc                                                                       >  2:8186842/1‑93 (MQ=255)

                  aCAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGc                                                                       <  1:3350957/93‑1 (MQ=255)

                    aaaTTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCg                                                                     <  1:3863689/93‑1 (MQ=255)

                     aaTTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCgg                                                                    <  1:7401339/93‑1 (MQ=255)

                     aaTTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCCTCACTTATCAGCGAACTAAACGAGTGCAATAACA‑TGCTTCATCCCgg                                                                    >  2:3885873/1‑93 (MQ=255)

                      aTTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCggg                                                                   >  1:580600/1‑93 (MQ=255)

                       ttCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGgc                                                                  <  2:5363793/93‑1 (MQ=255)

                        tcgtcgCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGgcc                                                                 >  1:8517461/1‑93 (MQ=255)

                          gtcgCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGgccgc                                                               >  2:4238901/1‑93 (MQ=255)

                            cgcgCTCGTCCACACTGGCTGGATGCGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCa                                                             >  1:1111144/1‑93 (MQ=255)

                             gcgcTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCaa                                                            <  2:5709416/93‑1 (MQ=255)

                               gcTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATg                                                          <  2:6451518/93‑1 (MQ=255)

                               gcTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATg                                                          <  2:7224402/93‑1 (MQ=255)

                                 tCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATgcg                                                        <  1:5699707/93‑1 (MQ=255)

                                  cGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATgcgc                                                       <  2:1716557/93‑1 (MQ=255)

                                  cGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATgcgc                                                       <  1:6070503/93‑1 (MQ=255)

                                    tCCAAACTGGCTGGATGTGCAACCGGCTTCTTCACTTATTAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTg                                                     >  1:5347642/1‑93 (MQ=255)

                                      cacaCTGGCTTGATGTGCAACCCGCTTGTTCACTTATGAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCCGGc                                                   >  2:2991539/1‑93 (MQ=255)

                                      cacaCTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGc                                                   >  1:3125333/1‑93 (MQ=255)

                                      cacaCTGGCTGGATGTGCAACCGACTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGc                                                   >  1:7349992/1‑93 (MQ=255)

                                       acacTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCa                                                  <  2:5928006/93‑1 (MQ=255)

                                         acTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑CACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAgg                                                >  2:7337435/1‑93 (MQ=255)

                                         acTGGCTGGATGGGCAACCGGCTTCTTCACTTATCAGCGTACTAAACGAGTGTAA‑AACACTGCTTCACTCCGGGCCTCCAACGGGCTGGCAgg                                                >  1:6704276/1‑93 (MQ=255)

                                          ctggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGga                                               <  2:4112181/93‑1 (MQ=255)

                                          ctggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGga                                               >  2:1606745/1‑93 (MQ=255)

                                            ggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGgaga                                             <  1:5965919/93‑1 (MQ=255)

                                              ctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATg                                           <  1:8456610/93‑1 (MQ=255)

                                                ggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGAc                                         >  2:8746935/1‑93 (MQ=255)

                                                   tgtgCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACACCGGGCCGCCAATGCGCTGGCAGGGGATGACCgg                                      >  1:279092/1‑93 (MQ=255)

                                                    gtgCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGa                                     <  1:3507036/93‑1 (MQ=255)

                                                      gCACCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGAcc                                   <  1:5417631/93‑1 (MQ=255)

                                                       cAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGAccc                                  <  2:3498597/93‑1 (MQ=255)

                                                       cAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGAccc                                  >  1:8552278/1‑93 (MQ=255)

                                                         aCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCAt                                >  2:1142208/1‑93 (MQ=255)

                                                          ccGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCCCGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATg                               <  2:6223201/93‑1 (MQ=255)

                                                          ccGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATg                               >  1:207175/1‑93 (MQ=255)

                                                          ccGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATg                               >  2:7194762/1‑93 (MQ=255)

                                                          ccGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGACGCCAATGCGCTGGCAGGAGATGACCGGACCCATg                               >  2:7372493/1‑93 (MQ=255)

                                                           cGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGa                              >  2:6873710/1‑93 (MQ=255)

                                                              cttcttCACTTATCAGCTAACTAAACCAGGGCAA‑AACACTGCTTCACGCCGGGCCACCAATGCTCTGGCAGGAGATGCCCGGACCCATGAAAg                           <  2:6035439/93‑1 (MQ=255)

                                                              cttcttCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAg                           <  2:8675260/93‑1 (MQ=255)

                                                              cttcttCACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAg                           <  2:5179535/93‑1 (MQ=255)

                                                               ttcttcACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAgg                          >  2:246827/1‑93 (MQ=255)

                                                               ttcttcACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAgg                          >  2:6181340/1‑93 (MQ=255)

                                                               ttcttcACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAgg                          >  1:159145/1‑93 (MQ=255)

                                                                 cttcACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAgggg                        >  1:2685328/1‑93 (MQ=255)

                                                                 cttcACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAgggg                        <  2:1504898/93‑1 (MQ=255)

                                                                 cttcACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAgggg                        <  2:3757239/93‑1 (MQ=255)

                                                                 cttcACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAgggg                        <  1:1977380/93‑1 (MQ=255)

                                                                  ttcACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGgc                       <  2:6709087/93‑1 (MQ=255)

                                                                  ttcACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGgc                       >  1:2570757/1‑93 (MQ=255)

                                                                   tcACTTATCAGCGAACTAAACGAGCCCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGgcg                      <  2:773947/93‑1 (MQ=255)

                                                                   tcACTTATCACCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGgcg                      <  2:6907779/93‑1 (MQ=255)

                                                                    cACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGgcgt                     <  1:473763/93‑1 (MQ=255)

                                                                    cACTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGgcgt                     <  2:5065075/93‑1 (MQ=255)

                                                                     aCTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGgcgtg                    >  2:3599788/1‑93 (MQ=255)

                                                                     aCTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGgcgtg                    >  2:3305/1‑93 (MQ=255)

                                                                      cTTATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGgcgtgc                   <  2:968761/93‑1 (MQ=255)

                                                                        tATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGgcgtgcgt                 <  1:4159168/93‑1 (MQ=255)

                                                                        tATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGgcgtgcgt                 <  1:3674796/93‑1 (MQ=255)

                                                                        tATCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGgcgtgcgt                 <  2:797858/93‑1 (MQ=255)

                                                                         aTCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGcgtgcgtg                <  2:561013/93‑1 (MQ=255)

                                                                          tCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTgg               <  1:7196958/93‑1 (MQ=255)

                                                                          tCAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTgg               >  2:4949404/1‑93 (MQ=255)

                                                                           cAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTggg              >  1:1299253/1‑93 (MQ=255)

                                                                           cAGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTggg              <  1:5178164/93‑1 (MQ=255)

                                                                            aGCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGgc             >  2:5678983/1‑93 (MQ=255)

                                                                             gCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGgcg            >  2:18322/1‑93 (MQ=255)

                                                                             gCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGgcg            >  2:1384710/1‑93 (MQ=255)

                                                                             gCGAACTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGgcg            >  1:881347/1‑93 (MQ=255)

                                                                                 aCTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATg        >  2:3195632/1‑93 (MQ=255)

                                                                                 aCTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATg        >  1:4343535/1‑93 (MQ=255)

                                                                                  cTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGt       <  2:2537518/93‑1 (MQ=255)

                                                                                  cTAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGt       <  1:5235259/93‑1 (MQ=255)

                                                                                   tAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCTCCAATGCTCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTc      <  1:4518095/93‑1 (MQ=255)

                                                                                   tAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTc      <  1:3260287/93‑1 (MQ=255)

                                                                                   tAAACGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTc      <  1:6331088/93‑1 (MQ=255)

                                                                                    aaaCGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCt     >  1:7490937/1‑93 (MQ=255)

                                                                                    aaaCGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCt     >  2:6384044/1‑93 (MQ=255)

                                                                                     aaCGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTg    <  1:7919291/93‑1 (MQ=255)

                                                                                      aCGAGGGCAA‑AACACTGCTTCACTCCGGGACGCCCATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCCCATGTCTGt   >  2:2138545/1‑93 (MQ=255)

                                                                                      aCGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGt   >  1:6938834/1‑93 (MQ=255)

                                                                                       cGAGGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGtt  <  2:8374427/93‑1 (MQ=255)

CAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAA‑AACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTT  >  NC_000913/547604‑547783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`