BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,583,050	G→A	Q446* (CAA→TAA)	hsdR ←	endonuclease R

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,583,050	0	G	A	99.8%	1524.4 / NA	425	Q446* (CAA→TAA)	hsdR	endonuclease R
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (207/217); total (207/218)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 433 total aligned reads displayed.

TTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGC  >  NC_000913/4582960‑4583139

tttCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACg                                                                                         <  2:4863229/93‑1 (MQ=255)

tttCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACg                                                                                         <  2:7290258/93‑1 (MQ=255)

 ttCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACgg                                                                                        <  2:1361211/93‑1 (MQ=255)

 ttCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACgg                                                                                        <  2:6367808/93‑1 (MQ=255)

    aaCCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTc                                                                                     <  2:4977137/93‑1 (MQ=255)

       cgcgcgGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCt                                                                                  >  1:6169554/1‑93 (MQ=255)

       cgcgcgGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCt                                                                                  <  1:2302151/93‑1 (MQ=255)

        gcgcgGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTg                                                                                 >  2:406159/1‑93 (MQ=255)

         cgcgGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGc                                                                                <  2:1796388/93‑1 (MQ=255)

         cgcgGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGc                                                                                <  2:300194/93‑1 (MQ=255)

         cgcgGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGc                                                                                <  1:1304262/93‑1 (MQ=255)

         cgcgGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGc                                                                                <  1:505912/93‑1 (MQ=255)

            ggACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGa                                                                             <  1:6969496/93‑1 (MQ=255)

             gACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAg                                                                            >  2:2203532/1‑93 (MQ=255)

             gACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAg                                                                            <  2:789168/93‑1 (MQ=255)

               cGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCa                                                                          >  1:4569407/1‑93 (MQ=255)

                 gCATCTTCGTGATAATAACGCAGGCCAAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCAtt                                                                        <  2:3523841/93‑1 (MQ=255)

                 gCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCCGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCAtt                                                                        <  1:7427824/93‑1 (MQ=255)

                    tCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTcc                                                                     >  1:7161474/1‑93 (MQ=255)

                    tCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTcc                                                                     >  2:4395220/1‑93 (MQ=255)

                     cTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCca                                                                    >  1:5984854/1‑93 (MQ=255)

                       tCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCcagc                                                                  >  2:7503112/1‑93 (MQ=255)

                       tCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCcagc                                                                  >  2:475571/1‑93 (MQ=255)

                       tCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCcagc                                                                  >  1:1468412/1‑93 (MQ=255)

                          tGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCagcagc                                                               <  1:2761159/93‑1 (MQ=255)

                           gATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGct                                                              >  1:5443412/1‑93 (MQ=255)

                            ataataACGCAGGCCCAGCTCGCTCATGCCAGGTTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGctc                                                             <  2:3256025/93‑1 (MQ=255)

                            ataataACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGctc                                                             <  2:3886595/93‑1 (MQ=255)

                             taataaCGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGctct                                                            >  1:8018798/1‑93 (MQ=255)

                             taataaCGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGctct                                                            <  2:5636504/93‑1 (MQ=255)

                              aataaCGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCtt                                                           <  2:6797356/93‑1 (MQ=255)

                               ataaCGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTc                                                          <  2:1955020/93‑1 (MQ=255)

                               ataaCGCAGGCCAAGAACGATCATGCCAGGGTTATCGGCAAACCACTAATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCATc                                                          <  2:7828158/93‑1 (MQ=255)

                                taaCGCAGGCCCAGCTCGCTCGTGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTcc                                                         <  2:3793971/93‑1 (MQ=255)

                                   cGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCggg                                                      <  2:8556952/93‑1 (MQ=255)

                                    gCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGc                                                     >  1:4131264/1‑93 (MQ=255)

                                    gCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGc                                                     >  2:2508065/1‑93 (MQ=255)

                                    gCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGc                                                     <  2:4477223/93‑1 (MQ=255)

                                     cAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCg                                                    >  1:2476245/1‑93 (MQ=255)

                                      aGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCgg                                                   <  2:3443912/93‑1 (MQ=255)

                                        gCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTg                                                 >  1:678515/1‑93 (MQ=255)

                                        gCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTg                                                 <  1:6102669/93‑1 (MQ=255)

                                         cccAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGc                                                <  1:3717521/93‑1 (MQ=255)

                                         cccAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGc                                                <  1:7932085/93‑1 (MQ=255)

                                          ccAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGcc                                               >  1:6401262/1‑93 (MQ=255)

                                           cAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCa                                              <  2:8696617/93‑1 (MQ=255)

                                            aGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCAc                                             >  1:5871534/1‑93 (MQ=255)

                                             gctcgctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACt                                            >  2:6667531/1‑93 (MQ=255)

                                             gctcgctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACt                                            <  2:2323187/93‑1 (MQ=255)

                                             gctcgctcATGCCAGGGTTATCGGCAAACCACTCATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACt                                            <  1:8465670/93‑1 (MQ=255)

                                              ctcgctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGGGCCACTc                                           >  1:3991852/1‑93 (MQ=255)

                                               tcgctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCg                                          <  2:2696420/93‑1 (MQ=255)

                                               tcgctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGATGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCg                                          >  2:5344175/1‑93 (MQ=255)

                                                cgctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCgg                                         <  1:441146/93‑1 (MQ=255)

                                                cgctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCgg                                         <  2:7077020/93‑1 (MQ=255)

                                                 gctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCggg                                        <  2:6143170/93‑1 (MQ=255)

                                                  ctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCgggg                                       >  2:3126909/1‑92 (MQ=255)

                                                    cATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTaa                                     <  2:5114107/93‑1 (MQ=255)

                                                    cATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTaa                                     >  2:6066074/1‑93 (MQ=255)

                                                    cATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTaa                                     >  2:5442302/1‑93 (MQ=255)

                                                    cATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTaa                                     <  2:4000906/93‑1 (MQ=255)

                                                      tGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAgg                                   >  1:7257502/1‑93 (MQ=255)

                                                        ccAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCt                                 >  1:7825344/1‑93 (MQ=255)

                                                        ccAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCt                                 <  2:4187627/93‑1 (MQ=255)

                                                         cAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCtt                                <  1:8065860/93‑1 (MQ=255)

                                                         cAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCtt                                <  1:5175387/93‑1 (MQ=255)

                                                          aGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCttt                               <  2:8604582/93‑1 (MQ=255)

                                                           gggTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTc                              <  1:767042/93‑1 (MQ=255)

                                                           gggTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTc                              <  2:2939215/93‑1 (MQ=255)

                                                           gggTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTc                              <  1:312378/93‑1 (MQ=255)

                                                           gggTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTc                              >  2:7388927/1‑93 (MQ=255)

                                                             gTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGa                            >  2:1172382/1‑93 (MQ=255)

                                                              ttATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGAc                           >  1:1970635/1‑93 (MQ=255)

                                                               tATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACa                          <  2:8202854/93‑1 (MQ=255)

                                                               tATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACa                          <  2:5748602/93‑1 (MQ=255)

                                                                  cGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACatat                       >  1:8396677/1‑93 (MQ=255)

                                                                   ggCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATAtt                      <  1:569132/93‑1 (MQ=255)

                                                                   ggCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATAtt                      >  2:5851569/1‑93 (MQ=255)

                                                                   ggCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATAtt                      >  2:7589564/1‑93 (MQ=255)

                                                                     cAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGc                    >  1:3476494/1‑93 (MQ=255)

                                                                      aaaCCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCg                   >  1:6594072/1‑93 (MQ=255)

                                                                      aaaCCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCg                   <  1:3259228/93‑1 (MQ=255)

                                                                      aaaCCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCg                   >  2:6919192/1‑93 (MQ=255)

                                                                      aaaCCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCg                   >  2:8106339/1‑93 (MQ=255)

                                                                       aaCCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCgg                  <  2:1697019/93‑1 (MQ=255)

                                                                        aCCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCggg                 <  1:1074912/93‑1 (MQ=255)

                                                                        aCCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCggg                 <  2:1432876/93‑1 (MQ=255)

                                                                         ccACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGt                <  1:4282647/93‑1 (MQ=255)

                                                                          cACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTa               <  2:7986025/93‑1 (MQ=255)

                                                                           aCTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTAt              >  1:1700856/1‑93 (MQ=255)

                                                                            cTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATc             >  1:4874016/1‑93 (MQ=255)

                                                                             tGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTGCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATca            >  1:8767323/1‑93 (MQ=255)

                                                                             tGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATca            >  1:1849585/1‑93 (MQ=255)

                                                                               aTTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacg          <  1:2619025/93‑1 (MQ=255)

                                                                               aTTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGGGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacg          >  1:7758451/1‑93 (MQ=255)

                                                                                ttCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGGATcacgc         >  2:8426822/1‑93 (MQ=255)

                                                                                 tCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacgca        >  1:4745394/1‑93 (MQ=255)

                                                                                  cTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacgcac       <  2:7131547/93‑1 (MQ=255)

                                                                                   tGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacgcacg      <  1:1214204/93‑1 (MQ=255)

                                                                                    gTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGt     >  2:5197851/1‑93 (MQ=255)

TTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGC  >  NC_000913/4582960‑4583139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`