BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	697,801	A→G	D200D (GAT→GAC)	asnB ←	asparagine synthetase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	697,801	0	A	G	99.6%	941.0 / NA	273	D200D (GAT→GAC)	asnB	asparagine synthetase B
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (133/139); minor base T (1/0); total (134/139)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 295 total aligned reads displayed.

GTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGT  >  NC_000913/697711‑697890

gTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTc                                                                                         >  2:7308475/1‑93 (MQ=255)

  aaGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGcgtcgt                                                                                       >  1:5092726/1‑93 (MQ=255)

  aaGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGcgtcgt                                                                                       <  1:4856489/93‑1 (MQ=255)

  aaGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGcgtcgt                                                                                       >  1:388935/1‑93 (MQ=255)

  aaGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGcgtcgt                                                                                       <  1:2246910/93‑1 (MQ=255)

   aGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTa                                                                                      >  1:8307797/1‑93 (MQ=255)

    ggCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAg                                                                                     <  1:7721632/93‑1 (MQ=255)

     gCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGt                                                                                    <  1:8625107/93‑1 (MQ=255)

     gCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGt                                                                                    <  1:2983045/93‑1 (MQ=255)

     gCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGt                                                                                    <  2:1329039/93‑1 (MQ=255)

     gCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGt                                                                                    <  2:6352382/93‑1 (MQ=255)

      cacaTCAGCCATCAGATGGCTTTTAACTGAATCTTCCAATGCCTGACGCAGCTCGTTTTTGTCGGTCGCGTTATCTTTCACCGCGTCGTAGTc                                                                                   <  2:7804708/93‑1 (MQ=255)

         atcagacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGaa                                                                                >  1:6546083/1‑93 (MQ=255)

          tcagacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAAc                                                                               <  2:4442766/93‑1 (MQ=255)

          tcagacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGGTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGacc                                                                               >  1:3926394/1‑91 (MQ=255)

           cagacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAAcc                                                                              <  1:4414239/93‑1 (MQ=255)

           cagacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAAcc                                                                              <  1:6471729/93‑1 (MQ=255)

            agacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCa                                                                             >  2:1712002/1‑93 (MQ=255)

            agacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCa                                                                             <  1:6060706/93‑1 (MQ=255)

             gacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAg                                                                            >  2:766820/1‑93 (MQ=255)

             gacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAg                                                                            >  1:1443798/1‑93 (MQ=255)

             aaaatcagaTGGATTTTTAATGAATCTTTCAGTTACTGATGCAGCTCGTTTTTGTTTGTCACGTTATCTTTCACCGCGTCGTAGACGAACCAg                                                                            <  2:8709205/90‑1 (MQ=255)

              acatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGt                                                                           >  1:6264905/1‑93 (MQ=255)

               catcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTc                                                                          >  1:261456/1‑93 (MQ=255)

                atcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTcg                                                                         <  2:1011990/93‑1 (MQ=255)

                 tcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTcgc                                                                        >  2:8760834/1‑93 (MQ=255)

                 tcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTcgc                                                                        >  1:5868663/1‑93 (MQ=255)

                   agaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCga                                                                      <  2:2431635/93‑1 (MQ=255)

                   agaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCga                                                                      >  2:4801939/1‑93 (MQ=255)

                   agaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCga                                                                      >  2:7099328/1‑93 (MQ=255)

                    gaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTTTCGGTTACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCgat                                                                     >  2:6424175/1‑93 (MQ=255)

                    gaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCgat                                                                     <  1:5737945/93‑1 (MQ=255)

                      tGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCgatga                                                                   >  2:6261317/1‑93 (MQ=255)

                       ggCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCgatgat                                                                  <  1:7091080/93‑1 (MQ=255)

                       ggCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCgatgat                                                                  <  1:5690655/93‑1 (MQ=255)

                       ggCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCgatgat                                                                  <  2:83213/93‑1 (MQ=255)

                         cTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTCCTCGAACCAGTCGCGATGATAg                                                                >  2:5932925/1‑93 (MQ=255)

                         cTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAg                                                                <  1:941216/93‑1 (MQ=255)

                         cTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTAAAGTTATCTTTCACCGCCTCGTCGTCGAACCAGTCGCAACGATAg                                                                >  1:2004162/1‑93 (MQ=255)

                          ttttAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAga                                                               >  1:5569972/1‑92 (MQ=255)

                            ttAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTaa                                                             <  2:8413664/93‑1 (MQ=255)

                               aCTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGaa                                                          >  1:1078630/1‑93 (MQ=255)

                               aCTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTGCACCGCGTCTTAGTCGAACCAGTCGCGATGATAGTAAGaa                                                          <  2:4342640/93‑1 (MQ=255)

                                cTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAAc                                                         >  1:1501464/1‑93 (MQ=255)

                                cTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAAc                                                         >  1:1123311/1‑93 (MQ=255)

                                cTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAAc                                                         <  2:5039730/93‑1 (MQ=255)

                                cTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAAc                                                         <  1:5207012/93‑1 (MQ=255)

                                   aaTCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGa                                                      <  1:6978260/93‑1 (MQ=255)

                                      cTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGAttt                                                   >  2:2716501/1‑93 (MQ=255)

                                       ttCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTc                                                  <  1:6725747/93‑1 (MQ=255)

                                       ttCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTc                                                  <  2:8018011/93‑1 (MQ=255)

                                       ttCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTc                                                  <  1:3986748/93‑1 (MQ=255)

                                         ccAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGc                                                >  2:4048153/1‑93 (MQ=255)

                                         ccAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGc                                                <  1:6318401/93‑1 (MQ=255)

                                          cAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGcc                                               >  2:6876222/1‑93 (MQ=255)

                                          cAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGcc                                               >  2:2539636/1‑93 (MQ=255)

                                           aGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCg                                              <  2:6178380/93‑1 (MQ=255)

                                           aGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTACCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCg                                              >  1:7328098/1‑93 (MQ=255)

                                            gTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCTTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGt                                             >  1:2387022/1‑93 (MQ=255)

                                            gTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGt                                             >  2:3777398/1‑93 (MQ=255)

                                            gTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGt                                             >  2:2861263/1‑93 (MQ=255)

                                             tGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTc                                            <  1:3107314/93‑1 (MQ=255)

                                             tGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTc                                            <  1:5455770/93‑1 (MQ=255)

                                              tccTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTcc                                           <  1:7877895/92‑1 (MQ=255)

                                              gCCTGACGCAGCTCGTTTTTGTCGGTCACTTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTcc                                           <  1:6352050/93‑1 (MQ=255)

                                               ccTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCt                                          >  2:2174379/1‑93 (MQ=255)

                                                cTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTg                                         <  1:4594487/93‑1 (MQ=255)

                                                 tGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTgg                                        <  2:5678949/93‑1 (MQ=255)

                                                      cAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCca                                   <  1:169170/93‑1 (MQ=255)

                                                       aGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCcac                                  <  2:363521/93‑1 (MQ=255)

                                                         cTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACaa                                >  2:1964850/1‑93 (MQ=255)

                                                          tCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACaaa                               >  2:6638094/1‑93 (MQ=255)

                                                          tCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACaaa                               >  1:2692588/1‑93 (MQ=255)

                                                          tCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACaaa                               >  2:1554755/1‑93 (MQ=255)

                                                             tttttGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGCTGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAg                            >  2:6557677/1‑93 (MQ=255)

                                                              ttttGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGc                           <  1:1245866/93‑1 (MQ=255)

                                                               tttGTCGGTCACGTTATATTTCACCGCGTCGTAGTCGAACCAGTAGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCt                          >  1:3374259/1‑93 (MQ=255)

                                                                ttGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAACTc                         >  2:3172697/1‑93 (MQ=255)

                                                                  gTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTccc                       >  2:6084260/1‑93 (MQ=255)

                                                                  gTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTccc                       >  2:2766279/1‑93 (MQ=255)

                                                                      gTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCccgcc                   <  1:4144404/93‑1 (MQ=255)

                                                                       tCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCccgccg                  <  2:1176231/93‑1 (MQ=255)

                                                                       tCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCccgccg                  <  2:5844354/93‑1 (MQ=255)

                                                                         aCGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCggg                >  2:3026127/1‑93 (MQ=255)

                                                                          cGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGa               <  1:3565151/93‑1 (MQ=255)

                                                                           gTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGaa              <  1:8078648/93‑1 (MQ=255)

                                                                            ttATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAAc             <  1:1058424/93‑1 (MQ=255)

                                                                            ttATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAAc             >  2:4951695/1‑93 (MQ=255)

                                                                             tATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAAct            >  2:1228092/1‑93 (MQ=255)

                                                                              aTCTTTCACCGCGTCGTAGTCGAGCCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAActc           <  1:4891484/93‑1 (MQ=255)

                                                                              aTCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAActc           >  1:1578253/1‑93 (MQ=255)

                                                                              aTCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAActc           <  2:2904559/93‑1 (MQ=255)

                                                                               tCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAActct          >  1:3755905/1‑93 (MQ=255)

                                                                               tCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAActct          >  1:6671937/1‑93 (MQ=255)

                                                                               tCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCCCCCCGCCGGGACctct          >  2:1785126/1‑93 (MQ=255)

                                                                                cTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCtt         <  2:3452731/93‑1 (MQ=255)

                                                                                 tttCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCttt        <  1:2169475/93‑1 (MQ=255)

                                                                                     cccGCGTCTTAGTCGAACCAGTCTCGATGATAGTAAGAACGGATTCCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATc    <  2:5446000/92‑1 (MQ=255)

                                                                                      ccGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCg   >  2:8192157/1‑93 (MQ=255)

                                                                                       cgcgTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGt  >  1:6899819/1‑93 (MQ=255)

GTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGT  >  NC_000913/697711‑697890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`