Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 704,130 A→G I322V (ATC→GTC)  nagE → PTS system, N‑acetylglucosamine‑specific enzyme IIABC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913704,1300AG99.5% 674.4 / NA 200I322V (ATC→GTC) nagEPTS system, N‑acetylglucosamine‑specific enzyme IIABC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (102/97);  minor base T (0/1);  total (102/98)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 213 total aligned reads displayed.

TACCTCCTGCACGCACTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTATCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTA  >  NC_000913/704040‑704218
                                                                                          |                                                                                        
 aCCTCCTGCACGCACTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCg                                                                                       >  2:4089091/1‑93 (MQ=255)
   ctcctGCACGCACTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGAc                                                                                     <  2:1277162/93‑1 (MQ=255)
   ctcctGCACGCACTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGAc                                                                                     <  1:5391842/93‑1 (MQ=255)
     cctGCACGCACTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTa                                                                                   <  2:8129133/93‑1 (MQ=255)
     cctGCACGCACTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTa                                                                                   >  2:80443/1‑93 (MQ=255)
       tGCACGCACTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTAcg                                                                                 >  1:2110521/1‑93 (MQ=255)
        gcacgcacTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTAcgc                                                                                <  1:1056627/93‑1 (MQ=255)
        gcacgcacTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTAcgc                                                                                <  2:1459956/93‑1 (MQ=255)
         cacgcacTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTAcgcg                                                                               >  1:7150491/1‑93 (MQ=255)
           cgcacTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGtt                                                                             <  2:5240929/93‑1 (MQ=255)
           cgcacTGCTGACCGGGATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGtt                                                                             <  1:7426139/93‑1 (MQ=255)
            gcacTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTg                                                                            >  1:3161410/1‑93 (MQ=255)
             cacTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGa                                                                           <  2:7270684/93‑1 (MQ=255)
             cacTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGa                                                                           >  1:1696556/1‑93 (MQ=255)
              acTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGAt                                                                          >  2:8285320/1‑93 (MQ=255)
               ctgctgACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATg                                                                         >  2:4660161/1‑93 (MQ=255)
               ctgctgACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATg                                                                         <  1:3336086/93‑1 (MQ=255)
                tgctgACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGt                                                                        >  2:4325569/1‑93 (MQ=255)
                cgctgACCGGTATCAGCCTGTTTGTGGCAACTCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGt                                                                        <  2:4016547/92‑1 (MQ=255)
                   tgACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGtata                                                                     <  1:500005/93‑1 (MQ=255)
                     aCCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAAc                                                                   <  1:8796437/93‑1 (MQ=255)
                      ccGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAAcc                                                                  >  1:5508718/1‑93 (MQ=255)
                       cGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCt                                                                 <  2:3633233/93‑1 (MQ=255)
                       cGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCt                                                                 >  1:1357204/1‑93 (MQ=255)
                        ggTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTg                                                                >  1:6618931/1‑93 (MQ=255)
                        ggTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTg                                                                >  2:6945798/1‑93 (MQ=255)
                        ggTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTg                                                                >  2:1116616/1‑93 (MQ=255)
                         gTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTgc                                                               >  1:1497327/1‑93 (MQ=255)
                         gTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTgc                                                               <  2:1197744/93‑1 (MQ=255)
                          tATTAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTgcc                                                              <  1:2361929/93‑1 (MQ=255)
                           aTCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTgccg                                                             >  1:2794428/1‑93 (MQ=255)
                               gCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTgccggccg                                                         >  2:2921019/1‑93 (MQ=255)
                                ccTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGgccgc                                                        <  2:3320907/93‑1 (MQ=255)
                                  tGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCgcca                                                      <  2:5884025/93‑1 (MQ=255)
                                  tGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCgcca                                                      >  1:997019/1‑93 (MQ=255)
                                  tGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCgcca                                                      <  1:8347872/93‑1 (MQ=255)
                                  tGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCgcca                                                      <  1:4444706/93‑1 (MQ=255)
                                    tttGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCgccagc                                                    >  1:8013348/1‑93 (MQ=255)
                                      tgtgGCAACGCTGCTGGGTATCCCCGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCgccagcca                                                  <  2:5955482/93‑1 (MQ=255)
                                          gCAACGCTGCTGGGTATCCACGCGGGCTTGTCTTTCTCTGCGGGGGCCGTCGACTACACGTTGATGTATAACTTGCCGGCCGCCAGCCAGAAc                                              >  2:3543942/1‑93 (MQ=255)
                                          gCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAAc                                              >  2:6445375/1‑93 (MQ=255)
                                              cGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCt                                          <  1:8731235/93‑1 (MQ=255)
                                               gctgctGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTg                                         >  2:3766899/1‑93 (MQ=255)
                                               gctgctGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTg                                         <  1:8430865/93‑1 (MQ=255)
                                               gctgctGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTg                                         >  2:1641759/1‑93 (MQ=255)
                                                ctgctgGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTgg                                        <  1:4005053/93‑1 (MQ=255)
                                                 tgctgGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGa                                       <  1:8566752/93‑1 (MQ=255)
                                                  gctgGGTATCCGCGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGAt                                      <  2:6279284/93‑1 (MQ=255)
                                                    tgGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGAtgc                                    >  2:7764491/1‑93 (MQ=255)
                                                     gggTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGAtgct                                   >  1:4163753/1‑93 (MQ=255)
                                                     gggTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGAtgct                                   >  2:6373638/1‑93 (MQ=255)
                                                      ggTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGAtgctg                                  >  2:7735662/1‑93 (MQ=255)
                                                      ggTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGAtgctg                                  >  2:320972/1‑93 (MQ=255)
                                                      ggTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGAtgctg                                  >  1:5459086/1‑93 (MQ=255)
                                                       gTATCCACGCGGGCTTCTCTTTCGCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGAtgctgc                                 <  2:3273492/93‑1 (MQ=255)
                                                        tATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATgctgct                                <  2:6852014/93‑1 (MQ=255)
                                                        tATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATgctgct                                >  2:5844778/1‑93 (MQ=255)
                                                        tATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATgctgct                                >  1:795536/1‑93 (MQ=255)
                                                         aTCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGctgctg                               <  1:1996543/93‑1 (MQ=255)
                                                          tCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTgg                              >  1:4815797/1‑93 (MQ=255)
                                                          tCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTgg                              >  2:5300731/1‑93 (MQ=255)
                                                               gcgGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTAAAACCTGCCGGCCGCCAGACAGAACGTCTGGATGCTGCTGGTGATg                         >  1:4399886/1‑93 (MQ=255)
                                                                cgGGTTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATgg                        <  1:6847462/93‑1 (MQ=255)
                                                                cgGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATgg                        <  1:3289677/93‑1 (MQ=255)
                                                                 gggCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATggg                       >  2:453879/1‑93 (MQ=255)
                                                                  ggCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGTGc                      <  2:6357643/93‑1 (MQ=255)
                                                                  ggCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGc                      >  1:6821299/1‑93 (MQ=255)
                                                                  ggCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTCCTGGTGATgggg                      >  1:6185730/1‑92 (MQ=255)
                                                                   gCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCg                     >  1:6320829/1‑93 (MQ=255)
                                                                   gCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCg                     >  2:2448671/1‑93 (MQ=255)
                                                                    cTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCATAACGTCTGGATGCTGCTGGTGATGGGCGt                    >  1:869295/1‑93 (MQ=255)
                                                                    cTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGt                    >  2:7037080/1‑93 (MQ=255)
                                                                     ttctctttctctGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCgct                   >  2:5274823/1‑91 (MQ=255)
                                                                      tctctttctctGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTa                  <  2:7559961/93‑1 (MQ=255)
                                                                      tctctttctatGCGGTGGCTGTCGACTACGCGTGGATGTATATCTTTCGGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTa                  <  2:6145760/93‑1 (MQ=255)
                                                                       ctctttctctGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTAt                 >  2:7227242/1‑93 (MQ=255)
                                                                       ctctttctctGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTAt                 >  1:3749321/1‑93 (MQ=255)
                                                                        tctttctctGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTAtc                <  2:3892528/93‑1 (MQ=255)
                                                                        tctttctctGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTAtc                >  2:4475938/1‑93 (MQ=255)
                                                                        tctttctctGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTAtc                >  1:3474491/1‑93 (MQ=255)
                                                                         ctttctctGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTAtct               <  1:2451299/93‑1 (MQ=255)
                                                                         ctttctctGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTAtct               >  1:6241359/1‑93 (MQ=255)
                                                                          tttctctGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTAtctt              >  1:1022576/1‑93 (MQ=255)
                                                                          tttctctGCGGGCGCTTTCGAATACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTAtctt              <  2:5480834/93‑1 (MQ=255)
                                                                           ttctctGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTAtcttc             <  2:6308821/93‑1 (MQ=255)
                                                                            tctctGCGGGGGCTGGCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTAtcttct            <  2:6615099/93‑1 (MQ=255)
                                                                             ctctGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATattctt           >  1:4465020/1‑93 (MQ=255)
                                                                              tctGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCttcttc          <  2:8302833/93‑1 (MQ=255)
                                                                                tGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGc        >  2:2317832/1‑93 (MQ=255)
                                                                                tGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGc        >  1:5173042/1‑93 (MQ=255)
                                                                                  ggggggCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTa      <  1:5858392/92‑1 (MQ=255)
                                                                                   gggggCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTAt     >  1:6544259/1‑93 (MQ=255)
                                                                                   gggggCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTAt     >  1:1014616/1‑93 (MQ=255)
                                                                                    gggtCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATc    <  2:7200318/93‑1 (MQ=255)
                                                                                    ggggCTGTCGACTACGCGTTGATGTATAGCCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATc    <  2:2517628/93‑1 (MQ=255)
                                                                                    ggggCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATc    <  1:1096171/93‑1 (MQ=255)
                                                                                    ggggCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATc    <  2:4785089/93‑1 (MQ=255)
                                                                                     gggCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCt   >  2:1849040/1‑93 (MQ=255)
                                                                                      ggCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTa  <  1:5608082/93‑1 (MQ=255)
                                                                                      ggCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTa  >  1:1262267/1‑93 (MQ=255)
                                                                                          |                                                                                        
TACCTCCTGCACGCACTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTATCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTA  >  NC_000913/704040‑704218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑