BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	704,311	A→G	N382S (AAC→AGC)	nagE →	PTS system, N‑acetylglucosamine‑specific enzyme IIABC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	704,311	0	A	G	100.0%	792.2 / NA	229	N382S (AAC→AGC)	nagE	PTS system, N‑acetylglucosamine‑specific enzyme IIABC
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (141/88); total (141/88)

Only 100 of 245 total aligned reads displayed.

GTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCT  >  NC_000913/704223‑704400

gtggtgTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCcagcaga                                                                                       >  1:5480442/1‑92 (MQ=255)

gtggtgTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCagcagc                                                                                       >  1:1107658/1‑93 (MQ=255)

gtggtgTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCagcagc                                                                                       >  2:6820298/1‑93 (MQ=255)

 tggtgTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAgcagca                                                                                      <  1:3344263/93‑1 (MQ=255)

 tggtgTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAgcagca                                                                                      >  1:1027235/1‑93 (MQ=255)

 cggtgTTAAGTTCGGTTATCCGCATGTTCACCCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAgcagca                                                                                      <  2:7257535/92‑1 (MQ=255)

    tgtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAaca                                                                                   <  1:80443/93‑1 (MQ=255)

     gtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAacac                                                                                  <  2:1789511/93‑1 (MQ=255)

      ttCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGGGATCGTTACTGAAGAAGCCAGCAGCAACACt                                                                                 >  1:5936019/1‑93 (MQ=255)

      ttCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACt                                                                                 >  1:5863705/1‑93 (MQ=255)

      ttCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACt                                                                                 >  1:4963836/1‑93 (MQ=255)

           ttcggTTATCCGCATGCTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTgaaga                                                                            >  1:4577007/4‑93 (MQ=255)

               gTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAggc                                                                        >  2:369464/1‑92 (MQ=255)

               gTTATCCGCATGTTCAACCTGAAAACGACGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGACGGt                                                                        >  2:8713649/1‑93 (MQ=255)

                 tATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTcg                                                                      >  1:7210549/1‑92 (MQ=255)

                  aTCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTg                                                                     >  2:5087057/1‑93 (MQ=255)

                   tCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGa                                                                    >  2:3321538/1‑93 (MQ=255)

                    ccGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGAc                                                                   <  1:4475938/93‑1 (MQ=255)

                      gCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTc                                                                 >  2:2859492/1‑93 (MQ=255)

                       cATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGCAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCa                                                                >  1:5720657/1‑93 (MQ=255)

                       cATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCa                                                                >  1:6039059/1‑93 (MQ=255)

                         tGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAAc                                                              >  2:1407924/1‑93 (MQ=255)

                         tGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAAc                                                              <  2:3646687/93‑1 (MQ=255)

                         tGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAAc                                                              >  2:4111373/1‑93 (MQ=255)

                            tCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTgg                                                           >  1:655044/1‑93 (MQ=255)

                              aaCCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGca                                                         >  2:133923/1‑93 (MQ=255)

                               aCCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTgcgag                                                        >  1:4132169/1‑89 (MQ=255)

                               aCCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGcaa                                                        >  2:1573366/1‑93 (MQ=255)

                                 cTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGcaacc                                                      <  1:5844779/93‑1 (MQ=255)

                                 cTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGcaacc                                                      >  2:8086191/1‑93 (MQ=255)

                                  tGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGTAGGTCTGACTCAACTGGcaacca                                                     >  2:4754343/1‑93 (MQ=255)

                                   gAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGcaaccaa                                                    >  2:3220348/1‑93 (MQ=255)

                                   gAAAACGCCGGGCCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGcaatcaa                                                    >  2:4056319/1‑93 (MQ=255)

                                     aaaCGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACt                                                  >  1:4206036/1‑93 (MQ=255)

                                     aaaCGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACt                                                  <  2:7102229/93‑1 (MQ=255)

                                      aaCGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACta                                                 <  2:795536/93‑1 (MQ=255)

                                          ccGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATAtt                                             >  2:8599524/1‑93 (MQ=255)

                                          ccGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATAtt                                             >  2:7386034/1‑93 (MQ=255)

                                           cGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATtg                                            >  2:6480488/1‑93 (MQ=255)

                                            gggTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATtgc                                           >  2:6966238/1‑93 (MQ=255)

                                            gggTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATtgc                                           <  2:4821380/93‑1 (MQ=255)

                                            gggTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATtgc                                           >  2:8751793/1‑93 (MQ=255)

                                             ggTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATtgct                                          <  1:4893590/93‑1 (MQ=255)

                                                cGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCg                                       >  2:6667009/1‑93 (MQ=255)

                                                 gTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCgg                                      >  2:7694494/1‑93 (MQ=255)

                                                 gTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCgg                                      <  1:3109030/93‑1 (MQ=255)

                                                   gAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGtt                                    >  2:5257716/1‑93 (MQ=255)

                                                   gAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGtt                                    <  1:5274823/93‑1 (MQ=255)

                                                   gAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGtt                                    <  1:2459031/93‑1 (MQ=255)

                                                   gAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGtt                                    >  1:6807123/1‑93 (MQ=255)

                                                   gAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGtt                                    >  1:5235128/1‑93 (MQ=255)

                                                    aaGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTg                                   <  2:7466425/93‑1 (MQ=255)

                                                    aaGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTg                                   <  1:3447785/93‑1 (MQ=255)

                                                    aaGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTg                                   <  1:7748048/93‑1 (MQ=255)

                                                      gATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTggg                                 >  1:3203638/1‑92 (MQ=255)

                                                      gATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTggc                                 >  2:6222639/1‑93 (MQ=255)

                                                      gATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTggc                                 <  2:1206722/93‑1 (MQ=255)

                                                        tAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTggcgg                               >  1:6122191/1‑93 (MQ=255)

                                                        tAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTggcgg                               >  1:2356890/1‑93 (MQ=255)

                                                        tAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTggcgg                               <  1:6908695/93‑1 (MQ=255)

                                                         aaaGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTggcggc                              <  1:3891692/93‑1 (MQ=255)

                                                         aaaGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTggcggc                              <  2:8360129/93‑1 (MQ=255)

                                                          aagaagACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCa                             >  2:1614907/1‑93 (MQ=255)

                                                          aagaagACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCa                             <  2:1434122/93‑1 (MQ=255)

                                                          aagaagACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCa                             <  1:2925843/93‑1 (MQ=255)

                                                          aagaagACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCa                             <  2:7228977/93‑1 (MQ=255)

                                                           agaagaCGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCCACCAACTATATTGCTGCGGTTGGCGGCAc                            <  2:5367241/93‑1 (MQ=255)

                                                           agaagaCGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCAc                            <  2:1497327/93‑1 (MQ=255)

                                                            gaagaCGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACt                           >  2:19828/1‑93 (MQ=255)

                                                            gaagaCGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACt                           <  2:1765342/93‑1 (MQ=255)

                                                              agaCGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGa                         <  1:1849040/93‑1 (MQ=255)

                                                               gaCGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGAc                        >  1:7070068/1‑93 (MQ=255)

                                                                   agaTCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAAcc                    >  2:2292102/1‑93 (MQ=255)

                                                                    gaTCTTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCt                   <  2:4399886/93‑1 (MQ=255)

                                                                    gaTCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCt                   <  2:824528/93‑1 (MQ=255)

                                                                    gaTCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCt                   >  1:5577500/1‑93 (MQ=255)

                                                                     aTCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTg                  >  1:716663/1‑93 (MQ=255)

                                                                     aTCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTg                  >  1:8620245/1‑93 (MQ=255)

                                                                         ttACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTTTATTGCTGCGGTTGGCGGCACTGACAACCTGAAAg              >  1:6464278/1‑93 (MQ=255)

                                                                           aCTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGca            >  2:6011383/1‑92 (MQ=255)

                                                                           aCTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCg            >  1:4024479/1‑93 (MQ=255)

                                                                             tGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGAt          >  1:3060898/1‑93 (MQ=255)

                                                                             tGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGAt          <  1:8305416/93‑1 (MQ=255)

                                                                              gaagaaGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGAtt         <  2:1262267/93‑1 (MQ=255)

                                                                              gaagaaGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGAtt         >  2:2368467/1‑93 (MQ=255)

                                                                              gaagaaGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGAtt         >  2:5680801/1‑93 (MQ=255)

                                                                               aagaagCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTg        >  1:6789181/1‑93 (MQ=255)

                                                                               aagaagCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTg        >  1:6262659/1‑93 (MQ=255)

                                                                               aagaagCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTg        >  2:2556760/1‑93 (MQ=255)

                                                                                agaagCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGa       <  2:8520304/93‑1 (MQ=255)

                                                                                agaagCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGa       >  2:1705525/1‑93 (MQ=255)

                                                                                 gaagCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGAc      >  1:7321808/1‑93 (MQ=255)

                                                                                 gaagCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGAc      <  2:2746060/93‑1 (MQ=255)

                                                                                 gaagCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGAc      >  1:471860/1‑93 (MQ=255)

                                                                                  aagCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACg     >  1:2101577/1‑93 (MQ=255)

                                                                                  aagCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACg     >  1:135650/1‑93 (MQ=255)

                                                                                    gCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGcc   >  1:2015823/1‑93 (MQ=255)

                                                                                     ccAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCt  >  2:6171302/1‑93 (MQ=255)

                                                                                     ccAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCt  >  2:100510/1‑93 (MQ=255)

                                                                                     ccAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCt  >  2:5518176/1‑93 (MQ=255)

GTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCT  >  NC_000913/704223‑704400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`