BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	709,321	A→G	I103M (ATA→ATG)	ybfN →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	709,321	0	A	G	99.1%	1130.0 / ‑2.8	330	I103M (ATA→ATG)	ybfN	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (167/160); total (168/162)
Fisher's exact test for biased strand distribution p-value = 6.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 347 total aligned reads displayed.

CGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTC  >  NC_000913/709231‑709410

cgTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGACATGCg                                                                                         >  1:4181114/1‑93 (MQ=255)

cgTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCg                                                                                         <  2:3357632/93‑1 (MQ=255)

 gTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATgcgc                                                                                        >  2:2186557/1‑93 (MQ=255)

  tACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCa                                                                                       <  1:1753753/93‑1 (MQ=255)

  tACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCa                                                                                       >  1:1067630/1‑93 (MQ=255)

   aCTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAg                                                                                      <  1:114492/93‑1 (MQ=255)

    cTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGt                                                                                     <  2:4429549/93‑1 (MQ=255)

     tGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGta                                                                                    <  2:708039/93‑1 (MQ=255)

     tGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGta                                                                                    >  2:5845008/1‑93 (MQ=255)

      ggATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTTTGATAAAGTCTGGCAGGAAATGCGCAGtaa                                                                                   >  1:588842/1‑93 (MQ=255)

      ggATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGtaa                                                                                   >  1:3114887/1‑93 (MQ=255)

      ggATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTCATAAAGTCTGGCAGGAAATGCGCAGtaa                                                                                   >  2:3712200/1‑93 (MQ=255)

       gATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGtaat                                                                                  <  2:6564836/93‑1 (MQ=255)

        aTTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGtaata                                                                                 <  1:1130487/93‑1 (MQ=255)

             cagcagTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAATTCTGGCAGGAAATGCGCAGTAATAACaaa                                                                            <  2:1692634/93‑1 (MQ=255)

             cagcagTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACaaa                                                                            <  1:4290264/93‑1 (MQ=255)

              agcagTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAAt                                                                           <  1:8624504/93‑1 (MQ=255)

               gcagTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATa                                                                          >  2:8445866/1‑93 (MQ=255)

               gcagTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATa                                                                          >  1:700955/1‑93 (MQ=255)

                cagTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATaa                                                                         <  1:8748465/93‑1 (MQ=255)

                cagTGTCTGCAAGCAACGCAAACCGGAAATGATCAGGCGATGAAAGCCGATTGTGATAAAGACTAACAGAAAATGCGCAGTAATAACAAATaa                                                                         <  1:7785077/93‑1 (MQ=255)

                 agTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAg                                                                        >  1:4401460/1‑93 (MQ=255)

                 agTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAg                                                                        >  1:6509633/1‑93 (MQ=255)

                  gtgtCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGt                                                                       <  1:7544332/93‑1 (MQ=255)

                  gtgtCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGt                                                                       >  1:4969997/1‑93 (MQ=255)

                  gtgtCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGt                                                                       <  2:7477784/93‑1 (MQ=255)

                  gtgtCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGt                                                                       >  2:2689176/1‑93 (MQ=255)

                  gtgtCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGt                                                                       >  2:6015744/1‑93 (MQ=255)

                  gtgtCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGGGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAgg                                                                       >  1:2802462/1‑92 (MQ=255)

                    gtCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTga                                                                     <  2:8159681/93‑1 (MQ=255)

                     tCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTgag                                                                    >  2:235516/1‑93 (MQ=255)

                     tCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTgag                                                                    <  1:2463313/93‑1 (MQ=255)

                      cggCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTgaga                                                                   <  2:8684475/91‑1 (MQ=255)

                      cTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTgaga                                                                   <  1:5079317/93‑1 (MQ=255)

                        gCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGc                                                                 >  2:3996693/1‑93 (MQ=255)

                        gCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGc                                                                 >  1:2296036/1‑93 (MQ=255)

                         cGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCt                                                                <  2:7550577/93‑1 (MQ=255)

                           tGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGt                                                              >  1:689098/1‑93 (MQ=255)

                            gCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTa                                                             >  1:3365514/1‑93 (MQ=255)

                              aaCGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAAc                                                           >  1:5361474/1‑93 (MQ=255)

                               aCGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAAct                                                          <  1:7183776/93‑1 (MQ=255)

                               aCGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAAct                                                          >  1:1822235/1‑93 (MQ=255)

                                cGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAActc                                                         <  1:3722902/93‑1 (MQ=255)

                                 gCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAActct                                                        <  2:7310109/93‑1 (MQ=255)

                                 gCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAActct                                                        <  2:3310164/93‑1 (MQ=255)

                                 gCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAActct                                                        <  1:1458049/93‑1 (MQ=255)

                                 gCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAActct                                                        <  1:3408525/93‑1 (MQ=255)

                                 gCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAActct                                                        <  1:3264823/93‑1 (MQ=255)

                                    aaCCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGc                                                     >  2:4952901/1‑93 (MQ=255)

                                     aCCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCt                                                    <  1:7309114/93‑1 (MQ=255)

                                     aCCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCt                                                    <  1:6764864/93‑1 (MQ=255)

                                        ggAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCtttt                                                 <  2:1558974/93‑1 (MQ=255)

                                        ggAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCtttt                                                 <  2:5448824/93‑1 (MQ=255)

                                          aaaTGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTTGCAGGAAATGCGCAATAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCt                                               >  1:2615935/1‑93 (MQ=255)

                                          aaaTGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCt                                               <  1:6163443/93‑1 (MQ=255)

                                          aaaTGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGAAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAGCTCTCGCTTTTCt                                               <  2:38954/93‑1 (MQ=255)

                                             tGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTAt                                            <  1:4514394/93‑1 (MQ=255)

                                             tGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTAt                                            >  1:8366137/1‑93 (MQ=255)

                                                tCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTc                                         <  2:1983237/93‑1 (MQ=255)

                                                acAGGCGGTGAAAGCCGATTGTGATAAAGGCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTc                                         <  1:6261586/92‑1 (MQ=255)

                                                 cAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTcc                                        >  2:5984016/1‑93 (MQ=255)

                                                  aGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGTAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTccc                                       <  2:6165809/93‑1 (MQ=255)

                                                  aGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGATGTAACTCTCGCTTTTCTTATTTacc                                       >  1:4100219/1‑93 (MQ=255)

                                                   ggCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCt                                      <  2:792038/93‑1 (MQ=255)

                                                   ggCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCt                                      >  1:3844082/1‑93 (MQ=255)

                                                    gCGTTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCtt                                     <  2:7688297/93‑1 (MQ=255)

                                                    gCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCtt                                     <  1:4836764/93‑1 (MQ=255)

                                                      ggTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGc                                   >  2:5142270/1‑93 (MQ=255)

                                                          aaaGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATaa                               >  1:2157817/1‑93 (MQ=255)

                                                           aaGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATaaa                              >  2:4206358/1‑93 (MQ=255)

                                                           aaGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATaaa                              >  1:8790643/1‑93 (MQ=255)

                                                            aGCCGATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATaaaa                             >  2:527208/1‑93 (MQ=255)

                                                                gATTGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGc                         >  2:7610450/1‑93 (MQ=255)

                                                                  ttGTGATACAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTACCTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCa                       <  2:7840821/93‑1 (MQ=255)

                                                                  ttGTGATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCa                       >  1:3447925/1‑93 (MQ=255)

                                                                   tgtgATAAAGTCTGGCAGGAAATTCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCaa                      <  1:2937832/93‑1 (MQ=255)

                                                                   tgtgATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCaa                      >  2:1770671/1‑93 (MQ=255)

                                                                    gtgATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAAc                     >  2:2966202/1‑93 (MQ=255)

                                                                    gtgATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAAc                     <  2:7232859/93‑1 (MQ=255)

                                                                     tgATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAAcc                    >  2:1268998/1‑93 (MQ=255)

                                                                     tgATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAAcc                    >  2:2512317/1‑93 (MQ=255)

                                                                      gATAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAAccc                   <  1:1684946/93‑1 (MQ=255)

                                                                       aTAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCg                  >  1:8092597/1‑93 (MQ=255)

                                                                        tAAAGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGc                 >  2:619240/1‑93 (MQ=255)

                                                                         aaaGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCa                >  2:6973456/1‑93 (MQ=255)

                                                                         aaaGTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTGCCCTTGCATAAAAAAGCCAACCCGCa                <  2:911082/93‑1 (MQ=255)

                                                                            gTCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGt             >  2:8413278/1‑93 (MQ=255)

                                                                             tCTGGCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGtt            <  2:4927129/93‑1 (MQ=255)

                                                                                ggCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGc         >  2:8188293/1‑93 (MQ=255)

                                                                                ggCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGc         <  1:5153636/93‑1 (MQ=255)

                                                                                 gCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAggctggct        >  2:314400/1‑93 (MQ=255)

                                                                                 gCAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCt        <  1:5987691/93‑1 (MQ=255)

                                                                                  cAGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCtt       <  2:1178667/93‑1 (MQ=255)

                                                                                   aGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCttt      >  2:6454455/1‑93 (MQ=255)

                                                                                   aGGAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCttt      <  2:4671387/93‑1 (MQ=255)

                                                                                     gAAATGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtc    <  2:1388094/93‑1 (MQ=255)

                                                                                      aaaTGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtct   >  1:5550018/1‑93 (MQ=255)

                                                                                      aaaTGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtct   >  2:7675076/1‑93 (MQ=255)

                                                                                      aaaTGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtct   >  1:459070/1‑93 (MQ=255)

                                                                                       aaTGCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtctc  >  2:3547913/1‑93 (MQ=255)

CGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTC  >  NC_000913/709231‑709410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`