Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 765,778 C→T A191V (GCC→GTC)  hrsA → PTS system fructose specific IIABC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913765,7780CT99.7% 1032.4 / NA 292A191V (GCC→GTC) hrsAPTS system fructose specific IIABC component
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (145/146);  total (146/146)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 301 total aligned reads displayed.

GCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAA  >  NC_000913/765686‑765866
                                                                                            |                                                                                        
  cTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCt                                                                                        >  1:980494/1‑93 (MQ=255)
  cTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCt                                                                                        <  1:7727902/93‑1 (MQ=255)
   tgatgaGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTg                                                                                       >  1:7854922/1‑93 (MQ=255)
   tgatgaGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTg                                                                                       >  1:5374366/1‑93 (MQ=255)
    gatgaGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGt                                                                                      >  1:4994442/1‑93 (MQ=255)
    gatgaGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGt                                                                                      >  1:1424151/1‑93 (MQ=255)
     atgaGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTc                                                                                     >  2:8607735/1‑93 (MQ=255)
     atgaGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTc                                                                                     <  1:1648090/93‑1 (MQ=255)
     atgaGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTc                                                                                     <  2:7848952/93‑1 (MQ=255)
      tgaGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTcc                                                                                    <  2:6692367/93‑1 (MQ=255)
       gaGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCcg                                                                                   >  1:5422979/1‑93 (MQ=255)
       gaGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCcg                                                                                   <  1:3498614/93‑1 (MQ=255)
        aGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCcgg                                                                                  <  1:2337646/93‑1 (MQ=255)
         gTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCcggc                                                                                 <  2:1273291/93‑1 (MQ=255)
              tctcGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTa                                                                            <  1:8165805/93‑1 (MQ=255)
                tcGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCACCGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTAtt                                                                          >  2:4002423/1‑93 (MQ=255)
                 cGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTg                                                                         <  2:3284938/93‑1 (MQ=255)
                   gcgcTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCt                                                                       >  1:7000656/1‑93 (MQ=255)
                     gcTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTca                                                                     >  2:5676485/1‑93 (MQ=255)
                     gcTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTca                                                                     >  1:6538720/1‑93 (MQ=255)
                      cTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACTCGCCGACTATCGTCTGCCTATCGGGCTGTCTGGCGGGGCCTGCTcac                                                                    >  2:3010168/1‑93 (MQ=255)
                        ggATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCacac                                                                  <  1:387595/93‑1 (MQ=255)
                           tGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCta                                                               >  1:642807/1‑93 (MQ=255)
                            gACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCtat                                                              >  1:2767353/1‑93 (MQ=255)
                             aCAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCtata                                                             <  1:7191866/93‑1 (MQ=255)
                             aCAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCtata                                                             <  1:7093424/93‑1 (MQ=255)
                             aCAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCtata                                                             <  1:4807060/93‑1 (MQ=255)
                              cAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTatat                                                            <  1:1223881/93‑1 (MQ=255)
                               aaGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATg                                                           <  1:1316692/93‑1 (MQ=255)
                                aGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGTTCACACCTATATgg                                                          >  1:2045666/1‑93 (MQ=255)
                                aGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATgg                                                          <  1:1944087/93‑1 (MQ=255)
                                 gggAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGc                                                         <  2:4448357/93‑1 (MQ=255)
                                 gggAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGc                                                         <  2:5775953/93‑1 (MQ=255)
                                  ggAGGCACGCCACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCt                                                        <  1:2163306/93‑1 (MQ=255)
                                  ggAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCt                                                        <  1:3229263/93‑1 (MQ=255)
                                  ggAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCt                                                        >  2:3544527/1‑93 (MQ=255)
                                   gAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTg                                                       <  2:6998287/93‑1 (MQ=255)
                                    aGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGc                                                      <  1:8673166/93‑1 (MQ=255)
                                    aGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGc                                                      >  1:5814723/1‑93 (MQ=255)
                                     ggCACGCAACCTTCTGTCTCTTTTTCCAACGCGCCATCTATCGTCTGCGTATCGGTCTGTCCGGCGTGTATTGCTCACACCTATATGGCTGCg                                                     <  2:1112925/93‑1 (MQ=255)
                                     ggCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCg                                                     >  2:4274652/1‑93 (MQ=255)
                                     ggCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCg                                                     >  2:2377984/1‑93 (MQ=255)
                                        aCGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGaa                                                  <  2:2210210/93‑1 (MQ=255)
                                          gCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAata                                                <  2:6038073/93‑1 (MQ=255)
                                           cAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAatat                                               >  2:6805543/1‑93 (MQ=255)
                                           cAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAatat                                               >  1:1848387/1‑93 (MQ=255)
                                            aaCCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATc                                              >  2:5105434/1‑93 (MQ=255)
                                            aaCCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATc                                              >  2:4563315/1‑93 (MQ=255)
                                             aCCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCt                                             >  1:6564121/1‑93 (MQ=255)
                                             aCCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCt                                             <  1:3076841/93‑1 (MQ=255)
                                              ccTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTg                                            >  2:7246390/1‑93 (MQ=255)
                                                ttCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGa                                          >  2:2452277/1‑93 (MQ=255)
                                                ttCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGa                                          >  1:5506695/1‑93 (MQ=255)
                                                   tGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGaaaa                                       >  1:8482300/1‑93 (MQ=255)
                                                   tGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGaaaa                                       <  2:6909357/93‑1 (MQ=255)
                                                    gCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGaaaaa                                      >  2:5299291/1‑93 (MQ=255)
                                                    gCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGaaaaa                                      >  2:6338260/1‑93 (MQ=255)
                                                    gCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGaaaaa                                      >  1:251307/1‑93 (MQ=255)
                                                    gCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGaaaaa                                      <  2:7714056/93‑1 (MQ=255)
                                                     ccTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAg                                     >  2:8074967/1‑93 (MQ=255)
                                                       tctTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGcc                                   >  2:386426/1‑93 (MQ=255)
                                                       tctTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGcc                                   >  2:4837415/1‑93 (MQ=255)
                                                       tctTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGcc                                   <  2:1777378/93‑1 (MQ=255)
                                                        ctTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCg                                  <  2:2890100/93‑1 (MQ=255)
                                                        ctTTTTATAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCAGGAAAAAGACg                                  >  2:8685704/1‑93 (MQ=255)
                                                         tttttCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCgg                                 >  1:3720215/1‑93 (MQ=255)
                                                         tttttCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCgg                                 <  1:4765833/93‑1 (MQ=255)
                                                         tttttCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCgg                                 >  1:8266606/1‑93 (MQ=255)
                                                           tttCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGAc                               >  2:5570439/1‑93 (MQ=255)
                                                           tttCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGAc                               <  1:8770042/93‑1 (MQ=255)
                                                             tCCAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGc                             >  2:6108811/1‑93 (MQ=255)
                                                              ccAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCa                            >  2:1668899/1‑93 (MQ=255)
                                                               cAACGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCaa                           >  2:8407076/1‑93 (MQ=255)
                                                                aaCGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATGTCTGGAAAACGCCGGACGCaaa                          >  1:4080899/1‑93 (MQ=255)
                                                                aaCGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCaaa                          <  1:5254719/93‑1 (MQ=255)
                                                                aaCGCGCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCaaa                          >  1:6106765/1‑93 (MQ=255)
                                                                 aCGCGCAAACTATCGTCTGCGTAACTGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAAc                         <  2:3609072/93‑1 (MQ=255)
                                                                  cgcgCCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACt                        <  1:2399152/93‑1 (MQ=255)
                                                                     gcCAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCgg                     <  1:7479932/93‑1 (MQ=255)
                                                                      ccAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGc                    >  2:1446535/1‑93 (MQ=255)
                                                                      ccAACTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGc                    >  2:6436775/1‑93 (MQ=255)
                                                                        aaCTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGt                  >  2:2644499/1‑93 (MQ=255)
                                                                        aaCGATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGt                  <  2:3904344/93‑1 (MQ=255)
                                                                         aCTATCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACACAAACTCGGCGTa                 <  1:150036/93‑1 (MQ=255)
                                                                          cTATCGTCGGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTaa                <  1:3362613/93‑1 (MQ=255)
                                                                            aTCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAAt              >  1:2183714/1‑93 (MQ=255)
                                                                            aTCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAAt              <  1:5905130/93‑1 (MQ=255)
                                                                            aTCGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAAt              <  2:291916/93‑1 (MQ=255)
                                                                              cGTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGt            <  1:6213339/93‑1 (MQ=255)
                                                                               gTCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGtt           >  1:2272422/1‑93 (MQ=255)
                                                                                tCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGttt          >  1:4510196/1‑93 (MQ=255)
                                                                                tCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGttt          >  2:3085180/1‑93 (MQ=255)
                                                                                tCTGCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGttt          >  1:1055365/1‑93 (MQ=255)
                                                                                   gCGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAGTGTCTGGAAAAAGCCGGAGGCAAACTCGGCGTAGTTGTTTACg       >  1:7327891/1‑93 (MQ=255)
                                                                                    cGTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGGATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGt      >  2:5388992/1‑93 (MQ=255)
                                                                                     gTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGtt     >  2:5916025/1‑93 (MQ=255)
                                                                                     gTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGtt     <  1:3091530/93‑1 (MQ=255)
                                                                                     gTAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGtt     <  1:2362355/93‑1 (MQ=255)
                                                                                      tAACGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTg    <  1:5996104/93‑1 (MQ=255)
                                                                                        aCGGTCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGaa  <  1:6908052/93‑1 (MQ=255)
                                                                                            |                                                                                        
GCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAA  >  NC_000913/765686‑765866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑