BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,747,389	Δ1 bp	coding (572/591 nt)	b4540 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,747,388	0	C	.	99.6%	1079.1 / ‑3.6	231	coding (571/591 nt)	b4540	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (1/0); new base . (121/109); total (122/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

Only 100 of 251 total aligned reads displayed.

CTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCCTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGATTCTGC  >  NC_000913/2747296‑2747476

ctTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATc                                                                                          <  1:3012335/93‑1 (MQ=255)

ctTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATc                                                                                          >  2:3744650/1‑93 (MQ=255)

ctTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATc                                                                                          >  2:2493054/1‑93 (MQ=255)

 tttGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATct                                                                                         <  1:2485898/93‑2 (MQ=255)

 tttGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATct                                                                                         <  2:3473485/93‑2 (MQ=255)

 tttGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATct                                                                                         <  2:1263264/93‑2 (MQ=255)

   tGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATctgg                                                                                       <  1:1476682/93‑4 (MQ=255)

    gAGCAAAGAAGAGTTGCTCTCTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTTGATctgga                                                                                      <  1:6302309/93‑5 (MQ=255)

       cAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGaaaa                                                                                  >  2:6353268/1‑93 (MQ=255)

        aaaGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGaaaaa                                                                                 <  2:1449086/93‑1 (MQ=255)

        aaaGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGaaaaa                                                                                 >  1:1481989/1‑93 (MQ=255)

        aaaGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGaaaaa                                                                                 >  2:5361036/1‑93 (MQ=255)

         aagaagAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGaaaaaa                                                                                >  2:215170/1‑93 (MQ=255)

         aagaagAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGaaaaaa                                                                                >  2:1751594/1‑93 (MQ=255)

          agaagaGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAAt                                                                               <  1:3805112/93‑1 (MQ=255)

           gaagaGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATg                                                                              >  2:3135303/1‑93 (MQ=255)

           gaagaGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATg                                                                              >  1:3904390/1‑93 (MQ=255)

             agagTTGCGCACTATCGTGCACGAATCACGCTCACCAATTTCCCGTCGCAATCAGGATATGCTGATGTCGGTGCTCGAT‑CTGGAACAAATGAc                                                                            >  1:2137448/1‑93 (MQ=255)

              gagTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGAcc                                                                           >  1:5191990/1‑93 (MQ=255)

               agTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCg                                                                          >  1:1111908/1‑93 (MQ=255)

                  tGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTtg                                                                       <  1:6354900/93‑1 (MQ=255)

                   gcgcACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTtga                                                                      >  1:2263310/1‑93 (MQ=255)

                    cgcACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTtgat                                                                     >  2:2200167/1‑93 (MQ=255)

                      cACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTtgatga                                                                   >  1:2871023/1‑93 (MQ=255)

                          aTCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGAcatc                                                               >  1:4412394/1‑93 (MQ=255)

                          aTCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGAcatc                                                               >  2:2592388/1‑93 (MQ=255)

                           tCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGAcatca                                                              <  1:5758156/93‑1 (MQ=255)

                           tCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGAcatca                                                              <  1:948578/93‑1 (MQ=255)

                              tGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATgg                                                           <  1:2924289/93‑1 (MQ=255)

                              tGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATgg                                                           <  1:3975779/93‑1 (MQ=255)

                               gCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGt                                                          >  1:4722093/1‑93 (MQ=255)

                                 aCGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGc                                                        >  1:5403214/1‑93 (MQ=255)

                                  cGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGcc                                                       <  2:4531052/93‑1 (MQ=255)

                                   gAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCcg                                                      >  2:3642518/1‑93 (MQ=255)

                                   gAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCcg                                                      >  2:5664194/1‑93 (MQ=255)

                                    aaTCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCcgc                                                     >  2:468695/1‑93 (MQ=255)

                                    aaTCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCcgc                                                     >  1:5114758/1‑93 (MQ=255)

                                    aaTCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGAAAAAAATGACCGTTGATGCCATCATGGTGCcgc                                                     <  1:2257019/93‑1 (MQ=255)

                                      tCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCgcgc                                                   >  2:4931504/1‑93 (MQ=255)

                                      tCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACAACATGGTGCCgcgc                                                   <  1:3145993/93‑1 (MQ=255)

                                       cACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCa                                                  >  2:4364367/1‑93 (MQ=255)

                                       cACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCa                                                  <  1:698872/93‑1 (MQ=255)

                                       cACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCa                                                  <  1:4918653/93‑1 (MQ=255)

                                        aCGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAg                                                 >  1:1202507/1‑93 (MQ=255)

                                        aCGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAg                                                 >  1:1021770/1‑93 (MQ=255)

                                        aCGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAg                                                 <  1:2432933/93‑1 (MQ=255)

                                         cGCTCACAAATTTCCCGTCGCATTCAGTATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGt                                                <  2:2442018/93‑1 (MQ=255)

                                         cGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGt                                                <  1:6436925/93‑1 (MQ=255)

                                           cTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGa                                              <  1:1567755/93‑1 (MQ=255)

                                           cTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGa                                              <  2:3085534/93‑1 (MQ=255)

                                            tCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGaa                                             >  1:332974/1‑93 (MQ=255)

                                              acaAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAAt                                           <  2:2814842/93‑1 (MQ=255)

                                               caAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAAtt                                          <  1:1623101/93‑1 (MQ=255)

                                                aaaTTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTa                                         <  1:2722747/93‑1 (MQ=255)

                                                 aaTTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTAt                                        <  1:3737308/93‑1 (MQ=255)

                                                  aTTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATc                                       <  1:6057565/93‑1 (MQ=255)

                                                  aTTTCCCGTCGCAATCACGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATc                                       <  2:1057307/93‑1 (MQ=255)

                                                   tttCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCg                                      <  1:2778894/93‑1 (MQ=255)

                                                    ttCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCgg                                     >  1:1795482/1‑93 (MQ=255)

                                                    ttCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCgg                                     >  1:2987941/1‑93 (MQ=255)

                                                      cccGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTa                                   >  1:4231589/1‑93 (MQ=255)

                                                       ccGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTAt                                  >  1:3630641/1‑93 (MQ=255)

                                                       ccGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTAt                                  <  1:5590875/93‑1 (MQ=255)

                                                        cGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTAtt                                 <  1:5930534/93‑1 (MQ=255)

                                                        cGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTAtt                                 >  2:5976413/1‑93 (MQ=255)

                                                         gTCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTg                                >  2:5647990/1‑93 (MQ=255)

                                                          tCGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGa                               >  1:4541993/1‑93 (MQ=255)

                                                           cGCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGat                              >  2:3853957/1‑93 (MQ=255)

                                                            gCAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGata                             >  1:2160493/1‑93 (MQ=255)

                                                             cAATCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGatat                            >  2:4749176/1‑93 (MQ=255)

                                                              aaTCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATc                           >  1:5469380/1‑93 (MQ=255)

                                                               aTCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCa                          <  2:2523231/93‑1 (MQ=255)

                                                               aTCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGAGGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCa                          >  2:3675271/1‑93 (MQ=255)

                                                                tCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCaa                         <  2:3595781/93‑1 (MQ=255)

                                                                tCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCaa                         <  2:5207178/93‑1 (MQ=255)

                                                                tCAGGATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCaa                         >  2:6286241/1‑93 (MQ=255)

                                                                   ggATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGa                      >  2:2701011/1‑93 (MQ=255)

                                                                    gATATGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGAt                     >  2:1589477/1‑93 (MQ=255)

                                                                     atatGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATg                    >  1:1909177/1‑93 (MQ=255)

                                                                     atatGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATg                    <  2:4133122/93‑1 (MQ=255)

                                                                       atGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGCAATTATCGGTATTGATATCAACGCTGAc                  >  2:2612426/1‑93 (MQ=255)

                                                                       atGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGAc                  >  1:5089294/1‑93 (MQ=255)

                                                                       atGCTGCTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGAc                  <  1:6118425/93‑1 (MQ=255)

                                                                        tgctgcTGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACt                 <  1:770747/93‑1 (MQ=255)

                                                                         gctgctGTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTg                <  2:6127674/93‑1 (MQ=255)

                                                                          ctgctgTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTgg               <  1:836892/93‑1 (MQ=255)

                                                                           tgctgTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGa              <  1:3719504/93‑1 (MQ=255)

                                                                            gctgTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGaa             <  1:3279241/93‑1 (MQ=255)

                                                                            gctgTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGaa             >  2:186185/1‑93 (MQ=255)

                                                                            gctgTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGaa             <  2:5882084/93‑1 (MQ=255)

                                                                              tgTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAAt           >  1:1895924/1‑93 (MQ=255)

                                                                               gTCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATc          <  1:1357697/93‑1 (MQ=255)

                                                                                tCGGTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCg         >  1:4534475/1‑93 (MQ=255)

                                                                                  ggTGCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGAt       >  1:1145604/1‑93 (MQ=255)

                                                                                     gCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGATTCt    <  2:591072/93‑1 (MQ=255)

                                                                                     gCTCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGATTCt    <  2:2037821/93‑1 (MQ=255)

                                                                                       tCGAT‑CTGGAAAGAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGATTCTgc  >  1:5151126/1‑93 (MQ=255)

                                                                                       tCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGATTCTgc  <  1:3244980/93‑1 (MQ=255)

                                                                                       tCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGATTCTgc  >  2:5838668/1‑93 (MQ=255)

                                                                                       tCGAT‑CTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGATTCTgc  <  2:2547045/93‑1 (MQ=255)

CTTTGAGCAAAGAAGAGTTGCGCACTATCGTGCACGAATCACGCTCACAAATTTCCCGTCGCAATCAGGATATGCTGCTGTCGGTGCTCGATCCTGGAAAAAATGACCGTTGATGACATCATGGTGCCGCGCAGTGAAATTATCGGTATTGATATCAACGATGACTGGAAATCGATTCTGC  >  NC_000913/2747296‑2747476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`