BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,092,722	(C)_5→4	coding (423/1026 nt)	yggR ←	YggR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,092,718	0	C	.	99.5%	984.7 / ‑3.3	210	coding (427/1026 nt)	yggR	YggR
Reads supporting (aligned to +/- strand): ref base C (0/0); major base . (99/110); minor base A (1/0); total (100/110)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 245 total aligned reads displayed.

AGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGC  >  NC_000913/3092626‑3092806

aGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGc                                                                                          <  1:4353168/93‑1 (MQ=255)

aGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGc                                                                                          >  2:6387117/1‑93 (MQ=255)

aGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGc                                                                                          >  1:3757431/1‑93 (MQ=255)

aGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGc                                                                                          >  1:1605734/1‑93 (MQ=255)

 ggATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGcc                                                                                         >  1:5888258/1‑93 (MQ=255)

 ggATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGcc                                                                                         >  1:6156646/1‑93 (MQ=255)

 ggATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATCGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGcc                                                                                         >  1:5244052/1‑93 (MQ=255)

  gATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGccc                                                                                        <  2:1557349/93‑1 (MQ=255)

   aTCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGcccc                                                                                       <  1:1968307/93‑1 (MQ=255)

     cTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGccccgt                                                                                     <  2:5454454/93‑3 (MQ=255)

     cTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGccccgt                                                                                     >  1:3315813/1‑91 (MQ=255)

      ttCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGccccgtc                                                                                    <  2:926341/93‑4 (MQ=255)

      ttCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGccccgtc                                                                                    >  1:5611487/1‑90 (MQ=255)

        ccAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCAc                                                                                 <  2:2312710/93‑1 (MQ=255)

        ccAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCAc                                                                                 >  2:4980911/1‑93 (MQ=255)

         cAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCAcc                                                                                <  2:1360743/93‑1 (MQ=255)

         cAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCAcc                                                                                >  1:2230818/1‑93 (MQ=255)

           gCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAg                                                                              >  1:3975381/1‑93 (MQ=255)

             gTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGaa                                                                            >  1:649814/1‑93 (MQ=255)

             gTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGaa                                                                            <  1:4160182/93‑1 (MQ=255)

              tCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAAt                                                                           >  2:1738976/1‑93 (MQ=255)

               cAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATc                                                                          <  2:2040993/93‑1 (MQ=255)

               cAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATc                                                                          <  1:2355209/93‑1 (MQ=255)

                aGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCa                                                                         <  1:5957750/93‑1 (MQ=255)

                aGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCa                                                                         <  2:3421648/93‑1 (MQ=255)

                 gAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAg                                                                        <  2:2631844/93‑1 (MQ=255)

                 gAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAg                                                                        >  2:1859595/1‑93 (MQ=255)

                  aaTATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAgg                                                                       <  1:3841132/93‑1 (MQ=255)

                  aaTATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAgg                                                                       >  1:4104646/1‑93 (MQ=255)

                   atatGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGc                                                                      >  1:1176504/1‑93 (MQ=255)

                   atatGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGc                                                                      >  1:3490373/1‑93 (MQ=255)

                    tatGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGcc                                                                     <  2:1677465/93‑1 (MQ=255)

                    tatGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGcc                                                                     >  2:2579362/1‑93 (MQ=255)

                    tatGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGcc                                                                     <  2:4355211/93‑1 (MQ=255)

                     atGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCa                                                                    >  1:2900459/1‑93 (MQ=255)

                     atGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCa                                                                    <  1:4980383/93‑1 (MQ=255)

                      tGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCAt                                                                   >  1:47112/1‑93 (MQ=255)

                      tGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCAt                                                                   >  1:3986909/1‑93 (MQ=255)

                      tGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCAt                                                                   <  2:5297231/93‑1 (MQ=255)

                      tGCGCATCGCCAGGTTGATTGAGATAGCCAGCCATCGCCGCCAGCGTGGTAGATTTGCCCCTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCAt                                                                   <  1:3367054/93‑1 (MQ=255)

                          cATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATtctc                                                               <  1:4757962/93‑1 (MQ=255)

                            tCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGc                                                             >  2:266609/1‑93 (MQ=255)

                            tCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGc                                                             <  1:3141195/93‑1 (MQ=255)

                            tCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGc                                                             <  2:5730668/93‑1 (MQ=255)

                            acGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGc                                                             <  1:1877598/92‑1 (MQ=255)

                              ggCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGctc                                                           >  2:5840350/1‑93 (MQ=255)

                              ggCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGctc                                                           >  2:1307623/1‑93 (MQ=255)

                               gCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGctct                                                          <  2:459148/93‑1 (MQ=255)

                               gCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGctct                                                          >  1:2153713/1‑93 (MQ=255)

                                gaTGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCtt                                                         <  2:6146445/92‑1 (MQ=255)

                                cATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCtt                                                         <  1:1747899/93‑1 (MQ=255)

                                cATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCtt                                                         >  1:1420247/1‑93 (MQ=255)

                                  tGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGa                                                       <  2:6057620/93‑1 (MQ=255)

                                   gTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAg                                                      <  2:5492508/93‑1 (MQ=255)

                                     tgattgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTa                                                    <  2:4559933/93‑1 (MQ=255)

                                      gattgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTaa                                                   >  1:2816894/1‑93 (MQ=255)

                                        ttgaGATAGCAAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAAtt                                                 <  1:3830437/93‑1 (MQ=255)

                                          gagaTAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTcc                                               <  1:357600/93‑1 (MQ=255)

                                            gaTAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCgg                                             >  1:5597675/1‑93 (MQ=255)

                                            gaTAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCgg                                             >  2:652610/1‑93 (MQ=255)

                                            gaTAGCCAACCATCGCCGCCAGCGTGGTAGACTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCgg                                             <  2:1643678/93‑1 (MQ=255)

                                             aTAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGc                                            >  2:478462/1‑93 (MQ=255)

                                               aGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCaa                                          >  2:5648201/1‑93 (MQ=255)

                                                   aaCCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATAcc                                      >  2:1077508/1‑93 (MQ=255)

                                                   aaCCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATAcc                                      <  2:5110669/93‑1 (MQ=255)

                                                    aCCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCg                                     <  1:3187016/93‑1 (MQ=255)

                                                    aCCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCg                                     <  2:4918803/93‑1 (MQ=255)

                                                    aCCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCg                                     >  2:3372772/1‑93 (MQ=255)

                                                     ccATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGt                                    <  2:2296664/93‑1 (MQ=255)

                                                     ccATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGGCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGt                                    <  1:4341792/93‑1 (MQ=255)

                                                      cATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGtt                                   <  2:2898484/93‑1 (MQ=255)

                                                      cATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGtt                                   <  2:4702305/93‑1 (MQ=255)

                                                      aaTCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGtt                                   <  2:2487683/92‑1 (MQ=255)

                                                        tCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTgg                                 <  2:2981940/93‑1 (MQ=255)

                                                        tCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTgg                                 <  1:5774776/93‑1 (MQ=255)

                                                         cgccgcCAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGt                                <  2:384263/93‑1 (MQ=255)

                                                            cgccAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTgcg                             >  1:934334/1‑93 (MQ=255)

                                                              ccAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGcc                           <  1:5571929/93‑1 (MQ=255)

                                                              ccAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGcc                           <  2:2078379/93‑1 (MQ=255)

                                                               cAGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCa                          >  2:3134640/1‑93 (MQ=255)

                                                                aGCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCaa                         <  2:845838/93‑1 (MQ=255)

                                                                 gCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAg                        >  2:1156625/1‑93 (MQ=255)

                                                                 gCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAg                        >  1:5659937/1‑93 (MQ=255)

                                                                 gCGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAg                        >  2:3566558/1‑93 (MQ=255)

                                                                  cGTGGTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGc                       <  1:351630/93‑1 (MQ=255)

                                                                     ggTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGt                    >  2:4321513/1‑93 (MQ=255)

                                                                      gTAGATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGtt                   >  2:4050655/1‑93 (MQ=255)

                                                                         gATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGa                >  1:5127276/1‑93 (MQ=255)

                                                                         gATTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGa                >  1:6090578/1‑93 (MQ=255)

                                                                          aTTTGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAg               >  1:5381379/1‑93 (MQ=255)

                                                                           tttGCCACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGCTCGAGc              >  1:6257859/1‑93 (MQ=255)

                                                                              gCCACTCCCCGTCG‑CCCCGTCACCAAAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTTGAGCTGc           >  1:1205376/1‑93 (MQ=255)

                                                                                cACTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTATCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCgg         >  1:4472795/1‑93 (MQ=255)

                                                                                 aCTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCggg        >  2:2638045/1‑93 (MQ=255)

                                                                                 aCTCCCCGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCggg        >  1:354846/1‑93 (MQ=255)

                                                                                   tCCCCGTCG‑CCCCGTCACCAGAATCAGGACATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCa      <  1:1028974/93‑1 (MQ=255)

                                                                                    ccccGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAg     <  1:741956/93‑1 (MQ=255)

                                                                                    ccccGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTCCGGGCAg     <  1:6357827/93‑1 (MQ=255)

                                                                                      ccGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTg   <  1:1509868/93‑1 (MQ=255)

                                                                                       cGTCG‑CCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGc  <  2:5351434/93‑1 (MQ=255)

AGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGC  >  NC_000913/3092626‑3092806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`