BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,542,643	Δ1 bp	intergenic (+46/‑22)	b4316 → / → fimD	Pil/FimD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,542,640	0	G	.	99.1%	981.8 / ‑0.4	211	intergenic (+43/‑25)	b4316/fimD	Pil/FimD
Reads supporting (aligned to +/- strand): ref base G (2/0); new base . (106/103); total (108/103)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 231 total aligned reads displayed.

TAAATGATTATGGCGCACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATATTCGTAAGCATC  >  NC_000913/4542548‑4542728

tAAATGATTATGGCGCACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCg                                                                                          >  2:3830185/1‑93 (MQ=255)

 aaaTGATTATGGCGCACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCgg                                                                                         >  1:3987272/1‑93 (MQ=255)

 aaaTGATTATGGCGCACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCgg                                                                                         <  1:2626438/93‑1 (MQ=255)

  aaTGATTATGGCGCACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCggg                                                                                        >  2:2515521/1‑93 (MQ=255)

    tGATTATGGCGCACTTAACCCCAAAATGACGGGCGTAATGGAGTACAGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCgggtg                                                                                      >  2:3438992/1‑91 (MQ=255)

      aTTATGGCGCACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGACTAAAAAGAATTGACTGCCgggtgat                                                                                    >  2:5627706/1‑89 (MQ=255)

       ttATGGCGCACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGAtt                                                                                  <  1:4046033/93‑1 (MQ=255)

          tGGCGCACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTa                                                                               <  1:1379571/93‑1 (MQ=255)

           ggCGCACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTaa                                                                              >  2:832032/1‑93 (MQ=255)

              gcACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGcc                                                                           >  2:5894454/1‑93 (MQ=255)

                aCTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGG‑GATTTTAAGCCgg                                                                         >  1:4777249/1‑93 (MQ=255)

                 cTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCgga                                                                        >  1:5232518/1‑93 (MQ=255)

                 cTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAAATTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCgga                                                                        <  1:2189910/93‑1 (MQ=255)

                  ttACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCggag                                                                       <  2:5697805/93‑1 (MQ=255)

                  ttACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCggag                                                                       <  1:351651/93‑1 (MQ=255)

                   tACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCggagg                                                                      >  1:6037072/1‑93 (MQ=255)

                   tACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCggagg                                                                      >  2:5479800/1‑93 (MQ=255)

                   tACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCggagg                                                                      <  2:2390629/93‑1 (MQ=255)

                    aCCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCggagga                                                                     <  1:5179568/93‑1 (MQ=255)

                     cccccAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGaa                                                                    <  2:2856497/93‑1 (MQ=255)

                      ccccAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGaat                                                                   >  2:22028/1‑93 (MQ=255)

                       cccAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGaata                                                                  >  2:1323646/1‑93 (MQ=255)

                       cccAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGaata                                                                  <  1:3675012/93‑1 (MQ=255)

                        ccAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGaataa                                                                 <  2:6021815/93‑1 (MQ=255)

                        ccAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGaataa                                                                 >  2:4302831/1‑93 (MQ=255)

                        ccAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGaataa                                                                 >  1:2732823/1‑93 (MQ=255)

                         cAAAATGTCGGGCGTAATGGAATAACGCAGGGGTAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGaataat                                                                <  1:4451257/93‑1 (MQ=255)

                          aaaaTGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATg                                                               >  1:5902135/1‑93 (MQ=255)

                          aaaaTGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATg                                                               <  1:4396421/93‑1 (MQ=255)

                           aaaTGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGt                                                              >  1:435685/1‑93 (MQ=255)

                           aaaTGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGt                                                              >  2:3140854/1‑93 (MQ=255)

                           aaaTGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGt                                                              >  1:281085/1‑93 (MQ=255)

                            aaTGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTc                                                             >  1:4833052/1‑93 (MQ=255)

                            aaTGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTc                                                             <  1:2517000/93‑1 (MQ=255)

                             aTGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCa                                                            >  1:1489915/1‑93 (MQ=255)

                              tGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCat                                                           >  1:5098850/1‑93 (MQ=255)

                               gACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCata                                                          >  1:5267792/1‑93 (MQ=255)

                               gACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCata                                                          >  1:3142803/1‑93 (MQ=255)

                                 cGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATc                                                        <  2:3969322/93‑1 (MQ=255)

                                   ggCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTg                                                      >  1:2116499/1‑93 (MQ=255)

                                   ggCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTg                                                      <  2:4798643/93‑1 (MQ=255)

                                   ggCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTg                                                      <  1:1017517/93‑1 (MQ=255)

                                    gCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGa                                                     <  1:6065244/93‑1 (MQ=255)

                                     cGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGaa                                                    <  1:4307279/93‑1 (MQ=255)

                                      gTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAAt                                                   >  1:869527/1‑93 (MQ=255)

                                        aaTGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAAttt                                                 >  1:1539148/1‑93 (MQ=255)

                                         aTGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTa                                                <  1:4721318/93‑1 (MQ=255)

                                             aaTAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGAc                                            <  1:5382167/93‑1 (MQ=255)

                                             aaTAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGAc                                            <  1:3613844/93‑1 (MQ=255)

                                             aaTAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGAc                                            <  2:1094864/93‑1 (MQ=255)

                                               tAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACtt                                          <  1:6467555/93‑1 (MQ=255)

                                               tAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACtt                                          <  1:4611987/93‑1 (MQ=255)

                                                aaCGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACttt                                         >  2:1707104/1‑93 (MQ=255)

                                                   gCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTAcc                                      <  2:5277113/93‑1 (MQ=255)

                                                    cAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCa                                     >  1:2652501/1‑93 (MQ=255)

                                                     aGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAg                                    <  2:1988785/93‑1 (MQ=255)

                                                     aGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAg                                    >  1:3302259/1‑93 (MQ=255)

                                                     aGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAg                                    >  1:5061177/1‑93 (MQ=255)

                                                      gggggAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGc                                   >  2:3516364/1‑93 (MQ=255)

                                                      gggggAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGc                                   >  1:3877000/1‑93 (MQ=255)

                                                      gggggAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGc                                   >  2:3372682/1‑93 (MQ=255)

                                                      gggggAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGc                                   <  2:178571/93‑1 (MQ=255)

                                                      gggggAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGc                                   >  2:2726750/1‑93 (MQ=255)

                                                      gggggAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGc                                   >  2:500536/1‑93 (MQ=255)

                                                        gggAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGa                                 >  2:3727288/1‑93 (MQ=255)

                                                         ggAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGaa                                >  1:3117531/1‑93 (MQ=255)

                                                          gAATTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGaaa                               >  1:3041972/1‑93 (MQ=255)

                                                           aaTTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGG‑GATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAac                              >  1:3810241/1‑93 (MQ=255)

                                                           aaTTTTTCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAac                              >  2:3614447/1‑93 (MQ=255)

                                                             tttttCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAacac                            <  2:5203527/93‑1 (MQ=255)

                                                              ttttCGCCTGAATAAAAAGAATTGACTTCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAacaca                           <  1:5510891/93‑1 (MQ=255)

                                                               tttCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAacacac                          <  2:646568/93‑1 (MQ=255)

                                                               tttCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAacacac                          <  1:904371/93‑1 (MQ=255)

                                                                 tCGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACaa                        <  1:3645709/93‑1 (MQ=255)

                                                                  cGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAAt                       <  1:6338166/93‑1 (MQ=255)

                                                                  cGCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAAt                       >  2:4475001/1‑93 (MQ=255)

                                                                   gCCTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATg                      >  2:4450683/1‑93 (MQ=255)

                                                                    ccTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGc                     <  2:5915239/93‑1 (MQ=255)

                                                                    ccTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGc                     >  1:5427493/1‑93 (MQ=255)

                                                                     cTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCt                    <  2:5968124/93‑1 (MQ=255)

                                                                     cTGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCt                    >  1:3231567/1‑93 (MQ=255)

                                                                      tGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCtt                   >  1:5334260/1‑93 (MQ=255)

                                                                      tGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCtt                   >  1:2299891/1‑93 (MQ=255)

                                                                      tGAATAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCtt                   <  2:4285264/93‑1 (MQ=255)

                                                                        aaTAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGc                 <  1:2044830/93‑1 (MQ=255)

                                                                          tAAAAAGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCat               >  2:905247/1‑93 (MQ=255)

                                                                           aaaaaGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCata              <  2:1607099/93‑1 (MQ=255)

                                                                            aaaaGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCatat             <  2:4373885/93‑1 (MQ=255)

                                                                             aaaGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATAtt            <  2:3314176/93‑1 (MQ=255)

                                                                              aaGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATATTc           >  1:5669268/1‑93 (MQ=255)

                                                                              aaGAATTGACTGCC‑GGGTGATTTTAAGCCGGAAGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATATTc           <  2:225975/93‑1 (MQ=255)

                                                                               aGAATTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATATTCg          >  2:4943640/1‑93 (MQ=255)

                                                                                  aTTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATATTCGTaa       <  2:3164140/93‑1 (MQ=255)

                                                                                  aTTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATATTCGTaa       >  2:4069088/1‑93 (MQ=255)

                                                                                  aTTGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATATTCGTaa       <  1:3157368/93‑1 (MQ=255)

                                                                                   ttGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATATTCGTAAg      >  2:5564418/1‑93 (MQ=255)

                                                                                   ttGACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATATTCGTAAg      >  2:3024148/1‑93 (MQ=255)

                                                                                     gACTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATATTCGTAAGCa    >  2:6211139/1‑93 (MQ=255)

                                                                                      aCTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATATTCGTAAGCAt   <  1:4917447/93‑1 (MQ=255)

                                                                                       cTGCC‑GGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATATTCGTAAGCATc  >  2:4010095/1‑93 (MQ=255)

TAAATGATTATGGCGCACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAATTTTTCGCCTGAATAAAAAGAATTGACTGCCGGGGTGATTTTAAGCCGGAGGAATAATGTCATATCTGAATTTAAGACTTTACCAGCGAAACACACAATGCTTGCATATTCGTAAGCATC  >  NC_000913/4542548‑4542728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`