BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,003,383	(G)_7→8	coding (184/681 nt)	ycbF →	putative chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,003,376	1	.	G	98.5%	456.0 / ‑0.5	134	coding (177/681 nt)	ycbF	putative chaperone
Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (114/18); total (115/19)
Fisher's exact test for biased strand distribution p-value = 2.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

Only 100 of 167 total aligned reads displayed.

GCAGACAGAGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCC‑‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCT  >  NC_000913/1003284‑1003464

gCAGACAGAGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGcc                                                                                            <  2:3897956/93‑1 (MQ=255)

 cagacagaGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCC‑‑g                                                                                         <  2:2771793/93‑1 (MQ=255)

 cagacagaGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCC‑‑g                                                                                         <  2:3981754/93‑1 (MQ=255)

  agacagaGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCC‑‑gg                                                                                        >  1:3877228/1‑93 (MQ=255)

   gacagaGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCC‑‑ggg                                                                                       >  1:1728262/1‑93 (MQ=255)

   gacagaGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCC‑‑ggg                                                                                       <  2:3614968/93‑1 (MQ=255)

   gacagaGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCC‑‑ggg                                                                                       <  2:4324921/93‑1 (MQ=255)

   gacagaGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCC‑‑ggg                                                                                       >  1:4673553/1‑93 (MQ=255)

     cagaGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCC‑‑ggggg                                                                                     >  1:2597950/1‑93 (MQ=255)

       gagaATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCC‑‑ggggggg                                                                                   >  1:5701294/1‑93 (MQ=255)

       gagaATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCC‑‑ggggggg                                                                                   >  1:1775661/1‑93 (MQ=255)

       gagaATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCC‑‑ggggggg                                                                                   >  2:5944524/1‑93 (MQ=255)

         gaATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCC‑‑gggggggga                                                                                 >  1:4640290/1‑91 (MQ=255)

          aaTCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCC‑‑ggggggggaa                                                                                >  2:1056019/1‑90 (MQ=255)

           atctatatctatTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCC‑‑ggggggggaag                                                                               <  1:2192721/93‑5 (MQ=255)

             cGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCg                                                                              >  1:4094688/1‑93 (MQ=255)

              gATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGt                                                                             >  2:4852740/1‑93 (MQ=255)

                tatCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCg                                                                           >  2:5098489/1‑93 (MQ=255)

                 atCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGa                                                                          >  2:6188995/1‑93 (MQ=255)

                   cTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACa                                                                        >  1:6252647/1‑93 (MQ=255)

                   cTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACa                                                                        >  2:1485894/1‑93 (MQ=255)

                    tATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAg                                                                       >  2:4441099/1‑93 (MQ=255)

                    tATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAg                                                                       >  2:4876170/1‑93 (MQ=255)

                    tATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAg                                                                       >  1:5456596/1‑93 (MQ=255)

                     aTTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGt                                                                      >  2:2386551/1‑93 (MQ=255)

                          aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAg                                                                 >  1:1153253/1‑93 (MQ=255)

                          aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAg                                                                 >  1:4431273/1‑93 (MQ=255)

                            tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGCGCCAgga                                                               >  2:5809408/1‑91 (MQ=255)

                             gACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCAc                                                              >  2:1129744/1‑93 (MQ=255)

                             gACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCAc                                                              >  2:5981754/1‑93 (MQ=255)

                               cTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCa                                                            >  1:2819622/1‑93 (MQ=255)

                                 aaTACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCAtt                                                          >  2:2203558/1‑93 (MQ=255)

                                   tACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTAc                                                        >  1:3680817/1‑93 (MQ=255)

                                   tACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTAc                                                        >  1:98596/1‑93 (MQ=255)

                                     ccAGTCGGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGATGCGTCGACAGTGCCAGCACCATTACTg                                                      >  1:5568901/1‑93 (MQ=255)

                                     ccAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTg                                                      >  1:4591870/1‑93 (MQ=255)

                                     ccAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTg                                                      >  1:2296026/1‑93 (MQ=255)

                                     ccAGTCAGGAACCCTGGCTTATAAACAGTAAAACCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTg                                                      <  2:1666831/93‑1 (MQ=255)

                                     caaGTCAGGAATCCTGTCTTATAAACAGAAAAATCACCCCCCAAACGCGTG‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTg                                                      <  1:6068141/91‑1 (MQ=255)

                                      cAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTgg                                                     >  1:45169/1‑93 (MQ=255)

                                       aGTCAGAATCCCTGTTTTATAAAAAGAAAAACCACCCGCCCACCGGGTG‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGc                                                    <  1:5918657/93‑1 (MQ=255)

                                       aGTAAGGAATCCTGGCTTATAAACAGAAAAACCAACCGCCCAAGGGGTG‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGc                                                    <  2:736441/93‑1 (MQ=255)

                                        gTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTggac                                                   >  1:4477237/1‑91 (MQ=255)

                                         tCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCg                                                  >  1:4658101/1‑93 (MQ=255)

                                           aGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCt                                                <  2:5250578/93‑1 (MQ=255)

                                           aGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCt                                                >  1:4972915/1‑93 (MQ=255)

                                             gAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTcc                                              >  2:202936/1‑93 (MQ=255)

                                             gAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTcc                                              >  1:2289376/1‑93 (MQ=255)

                                             gAATCCTGGCTTATAAACAGTAAAATCACCCGCCCAGCGTTTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTcc                                              <  2:2939651/93‑1 (MQ=255)

                                                tCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGTAGCGTCGACAGTGCCAGCACCATTACTGGCGGCTCCGcc                                           >  2:6087931/1‑93 (MQ=255)

                                                tCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGcc                                           >  2:6024196/1‑93 (MQ=255)

                                                tCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGcc                                           >  2:5681178/1‑93 (MQ=255)

                                                 ccTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCC‑‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCAc                                         >  2:2063701/1‑93 (MQ=255)

                                                  cTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCAc                                         >  1:5330622/1‑93 (MQ=255)

                                                  cTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCAc                                         >  1:3575356/1‑93 (MQ=255)

                                                  cTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCAc                                         >  2:5200298/1‑93 (MQ=255)

                                                     gCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTa                                      >  1:1155176/1‑93 (MQ=255)

                                                       ttattaACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTAtt                                    >  2:5390815/1‑93 (MQ=255)

                                                       ttattaACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTAtt                                    >  2:5955941/1‑93 (MQ=255)

                                                        tattaACAGTAAAATCAACCGCCCACCGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATtc                                   <  1:4009837/93‑1 (MQ=255)

                                                        tattaACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCGGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTAtt                                    >  1:4146015/1‑93 (MQ=255)

                                                        tattaACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATtc                                   >  1:1959933/1‑93 (MQ=255)

                                                         attaACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATtct                                  >  2:3358158/1‑93 (MQ=255)

                                                         attaACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGATCCCCCACTTATtct                                  >  1:3764741/1‑93 (MQ=255)

                                                          ttaACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATtctc                                 >  2:1748008/1‑93 (MQ=255)

                                                          ttaACAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATtctc                                 >  2:1600460/1‑93 (MQ=255)

                                                           taACAGTAAAACAAACCCCCCAAGGCGTTGGGGCG‑‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTcc                                <  2:4111904/93‑1 (MQ=255)

                                                             aCAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTg                              >  2:2323873/1‑93 (MQ=255)

                                                             aCAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTg                              >  1:515579/1‑93 (MQ=255)

                                                             aCAGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTg                              >  1:3252652/1‑93 (MQ=255)

                                                               aGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGaa                            >  2:6447724/1‑93 (MQ=255)

                                                               aGTAAAATCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGaa                            >  1:2998050/1‑93 (MQ=255)

                                                                agaaaaTGCACCCCCCAACGGATT‑GGGGCAGGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGaa                            <  1:499978/91‑1 (MQ=255)

                                                                  aaacTCACCCGCCCAACGCGTT‑GGCCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGcc                         <  1:863168/93‑1 (MQ=255)

                                                                  aaaaTCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGCAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGcc                         >  1:2064231/1‑93 (MQ=255)

                                                                  aaaaTCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGcc                         >  2:5543430/1‑93 (MQ=255)

                                                                  aaaaTCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGcc                         >  1:922067/1‑93 (MQ=255)

                                                                   aaaTCAACCGCCCACCGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGccc                        <  1:2014685/93‑1 (MQ=255)

                                                                    aaTCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCg                       >  1:2844723/1‑93 (MQ=255)

                                                                      tCAACCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGt                     >  1:2314956/1‑93 (MQ=255)

                                                                        aaCCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTag                   >  2:6064521/1‑92 (MQ=255)

                                                                        aaCCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTAc                   >  2:3708643/1‑93 (MQ=255)

                                                                         aCCGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACg                  >  2:2495351/1‑93 (MQ=255)

                                                                          ccGCCCAACGGGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGa                 <  1:6448040/93‑1 (MQ=255)

                                                                          ccGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGa                 >  1:5685666/1‑93 (MQ=255)

                                                                          ccGCCCAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGa                 >  2:2100366/1‑93 (MQ=255)

                                                                             cccAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAg              >  1:6053249/1‑93 (MQ=255)

                                                                             cccAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAg              >  1:1629431/1‑93 (MQ=255)

                                                                              ccAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAgg             >  2:3075501/1‑93 (MQ=255)

                                                                              ccAACGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAgg             >  2:2608838/1‑93 (MQ=255)

                                                                                aaCGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCa           >  2:3765279/1‑93 (MQ=255)

                                                                                aaCGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCa           >  2:2034351/1‑93 (MQ=255)

                                                                                aaCGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCa           >  2:3037493/1‑93 (MQ=255)

                                                                                aaCGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCa           >  1:6448948/1‑93 (MQ=255)

                                                                                 aCGCGTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCa           <  1:1804054/92‑1 (MQ=255)

                                                                                  cgcgTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACg         >  2:2833180/1‑93 (MQ=255)

                                                                                     gTT‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTg      >  1:662974/1‑93 (MQ=255)

                                                                                      tt‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTgc     >  2:621954/1‑93 (MQ=255)

                                                                                       t‑GGGCCG‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTgcg    >  1:22303/1‑93 (MQ=255)

                                                                                         ggccc‑‑GGGGGGGAAGCGTCGACAGTCCCAGCACCATTACTGGCCGCTCCGACACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCt  <  2:4025797/90‑1 (MQ=255)

GCAGACAGAGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTT‑GGGCC‑‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCT  >  NC_000913/1003284‑1003464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`