BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,207,165	(G)_7→8	coding (442/630 nt)	ycfK →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,207,158	1	.	G	97.7%	589.7 / 5.6	173	coding (435/630 nt)	ycfK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (2/2); new base G (64/105); total (66/107)
Fisher's exact test for biased strand distribution p-value = 6.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

Only 100 of 213 total aligned reads displayed.

ACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑T‑GG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCA  >  NC_000913/1207066‑1207245

aCCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCctc‑t                                                                                               <  2:4609994/93‑1 (MQ=255)

 ccGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑T‑g                                                                                             <  2:3605800/93‑1 (MQ=255)

  cGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑T‑gg                                                                                            <  2:4926290/93‑1 (MQ=255)

   ggTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑T‑gg‑g                                                                                          <  1:2445553/93‑1 (MQ=255)

    gTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑T‑gg‑g‑g                                                                                        <  2:5531575/93‑1 (MQ=255)

    gTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑T‑gg‑g‑g                                                                                        >  1:2651742/1‑93 (MQ=255)

     tttCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑T‑gg‑g‑g‑g                                                                                      <  1:4234679/93‑1 (MQ=255)

       tCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑T‑gg‑g‑g‑g‑g‑g                                                                                  >  2:6209060/1‑93 (MQ=255)

       tCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑T‑gg‑g‑g‑g‑g‑g                                                                                  >  1:622100/1‑93 (MQ=255)

       tCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑T‑gg‑g‑g‑g‑g‑g                                                                                  >  1:5731901/1‑93 (MQ=255)

         aTAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑T‑gg‑g‑g‑g‑g‑gga                                                                                >  2:4643150/1‑91 (MQ=255)

         aTAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑T‑gg‑g‑g‑g‑g‑gga                                                                                >  2:6124608/1‑91 (MQ=255)

          tAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑T‑gg‑g‑g‑g‑g‑ggat                                                                               >  1:2373480/1‑90 (MQ=255)

           aGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑T‑gg‑g‑g‑g‑g‑ggatg                                                                              >  1:5363609/1‑89 (MQ=255)

               ataCCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGaaa                                                                           <  2:736136/93‑1 (MQ=255)

               ataCCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGaaa                                                                           <  2:4263410/93‑1 (MQ=255)

                taCCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGTGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAAt                                                                          <  2:1240912/93‑1 (MQ=255)

                 aCCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATa                                                                         >  2:1746193/1‑93 (MQ=255)

                   cGGTGATCATGGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACa                                                                       <  2:1107107/93‑1 (MQ=255)

                    ggTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAg                                                                      >  2:638666/1‑93 (MQ=255)

                     gTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGt                                                                     <  1:3860529/93‑1 (MQ=255)

                     gTTATGATTGGTGGTATTGACCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGt                                                                     <  1:6462271/93‑1 (MQ=255)

                       tATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GGTGGAAATACAGTTg                                                                   >  2:4778376/1‑93 (MQ=255)

                       tATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTg                                                                   <  1:116016/93‑1 (MQ=255)

                       tATGATTGGGTGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTg                                                                   <  1:3529929/93‑1 (MQ=255)

                         tGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTAGAATGCCGCAAAAGCAGCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTa                                                                 <  1:446110/93‑1 (MQ=255)

                          gATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTAt                                                                >  1:2714940/1‑93 (MQ=255)

                          gATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTAt                                                                >  1:5456928/1‑93 (MQ=255)

                           aTTGGTGGTATTGATCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTAtt                                                               <  1:362254/93‑1 (MQ=255)

                           aTTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTAtt                                                               >  1:2801490/1‑93 (MQ=255)

                            ttGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTc                                                              >  1:3251877/1‑93 (MQ=255)

                             tggtggTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTcc                                                             <  1:149874/93‑1 (MQ=255)

                              ggtggtATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTccc                                                            <  1:5503059/93‑1 (MQ=255)

                               gtggtATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCCCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCg                                                           <  1:955222/93‑1 (MQ=255)

                                tggtATTGAGCAATCAGTAATCATTCAGTGGGGGCGGAATGCCGCAAAAGCATCTGCCTC‑T‑GG‑GTG‑G‑G‑GATGGTAATACAGGTGTATTcctgt                                                          >  2:4000309/1‑90 (MQ=255)

                                 ggtATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGt                                                         <  1:3065312/93‑1 (MQ=255)

                                  gtATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGtt                                                        <  1:4380241/93‑1 (MQ=255)

                                  gtATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGtt                                                        >  1:6370673/1‑93 (MQ=255)

                                   tATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTg                                                       >  1:6288359/1‑93 (MQ=255)

                                    aTTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAAAACAGTTGTATTCCCGGttcg                                                      >  2:5193402/1‑91 (MQ=255)

                                     ttGAGCAATCAGTAATCATTCAGTGGGGGGGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCg                                                     >  1:4175428/1‑93 (MQ=255)

                                       gAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGtt                                                   >  1:5140349/1‑93 (MQ=255)

                                       gAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGtt                                                   >  2:5423950/1‑93 (MQ=255)

                                        aGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGttt                                                  <  1:2853005/93‑1 (MQ=255)

                                         gCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAACTACAGTTGTCTTCCCGGTTGCGTTta                                                 >  1:3039289/1‑93 (MQ=255)

                                          cAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATAGAAATACAGTTGTATTCCCGGTTGCGTTtaa                                                <  2:3756076/93‑1 (MQ=255)

                                           aaTCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTtaat                                               >  1:5010291/1‑93 (MQ=255)

                                            aTCAGTAATCATTCAGTGGGGGTGGAGTGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTtaata                                              <  2:3079253/93‑1 (MQ=255)

                                            aTCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTtaata                                              >  2:615913/1‑93 (MQ=255)

                                            aTCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTtaata                                              >  2:2397614/1‑93 (MQ=255)

                                            aTCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑T‑GG‑G‑G‑GCG‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTtaata                                              <  2:1498993/93‑1 (MQ=255)

                                             tCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTtaataa                                             <  2:3270541/93‑1 (MQ=255)

                                               aGTAATCATTCAGTGGGGGTGGAATGTCGCAAAAGCATCTGCCTC‑T‑GG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGc                                          <  1:5200920/93‑1 (MQ=255)

                                               aGTAAGCATTCGGGGGGGGGGGAGTGCCGCACAAGCATCTGCGTCTT‑GG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATg                                           <  2:1370115/93‑1 (MQ=255)

                                                 tagtCATTCAGTGGGGGTGGAAGGTCTCAAAAGCGTCTGCCTC‑T‑GG‑G‑G‑G‑GTGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGcc                                         <  2:3131357/90‑1 (MQ=255)

                                                 tAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGcc                                         <  1:5949256/93‑1 (MQ=255)

                                                 tAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGcc                                         <  2:2276064/93‑1 (MQ=255)

                                                 tAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGcc                                         <  2:584036/93‑1 (MQ=255)

                                                 tAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCGGCGTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGcc                                         <  2:2578683/93‑1 (MQ=255)

                                                 gaaTCATTCCTGGGGGGTGGGATTCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGcc                                         <  2:1909750/92‑1 (MQ=255)

                                                   aTCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCtg                                       <  1:390734/93‑1 (MQ=255)

                                                     cATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCtgtg                                     >  2:6252272/1‑93 (MQ=255)

                                                      aTTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTgtgt                                    <  2:3228201/93‑1 (MQ=255)

                                                      aTTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTgtgt                                    <  2:4449682/93‑1 (MQ=255)

                                                       ttCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGtt                                   <  2:5847346/93‑1 (MQ=255)

                                                       tgcAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGtt                                   <  2:2151693/91‑1 (MQ=255)

                                                         cAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGc                                 >  2:5700893/1‑93 (MQ=255)

                                                          aGTGGGGGGGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGcc                                <  1:2117376/93‑1 (MQ=255)

                                                           gTGGGGGTGGAATGGCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCg                               >  1:1638309/1‑93 (MQ=255)

                                                           gTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCg                               <  2:2991349/93‑1 (MQ=255)

                                                           gTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCg                               >  2:3470128/1‑93 (MQ=255)

                                                           gTGGGGGTGGAATACCGCAAAAGCATCTGCCTC‑T‑GG‑G‑GCG‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCg                               <  2:4703003/93‑1 (MQ=255)

                                                            tGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCgt                              <  2:2411729/93‑1 (MQ=255)

                                                            tGGGGGTGGAACGGCGCAAACGCATGTGCCTC‑T‑GGTG‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCgt                              <  1:1373254/93‑1 (MQ=255)

                                                             gggggTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCgtt                             <  2:5057209/93‑1 (MQ=255)

                                                             gggggTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCgtt                             <  1:5847461/93‑1 (MQ=255)

                                                              ggggTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCgttg                            <  2:440670/93‑1 (MQ=255)

                                                               gggTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCgttgt                           <  2:3104793/93‑1 (MQ=255)

                                                                ggTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCgttgtt                          <  2:896441/93‑1 (MQ=255)

                                                                ggTGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCgttgtt                          <  1:4803327/93‑1 (MQ=255)

                                                                  tGGAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCTGTTGTTGc                        <  1:6328082/93‑1 (MQ=255)

                                                                   ggAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCa                       <  2:5207494/93‑1 (MQ=255)

                                                                   ggAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCa                       <  2:430703/93‑1 (MQ=255)

                                                                    gAATGCCGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCaa                      >  1:5594771/1‑93 (MQ=255)

                                                                       tGCCGCAAAAGTATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAAtt                   >  1:3489207/1‑93 (MQ=255)

                                                                         ccGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTAt                 <  2:3315800/93‑1 (MQ=255)

                                                                          cGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATg                >  1:698767/1‑93 (MQ=255)

                                                                          cGCAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATg                <  1:1843087/93‑1 (MQ=255)

                                                                            cAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGAc              <  2:3925124/93‑1 (MQ=255)

                                                                            cAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGAc              <  1:1976689/93‑1 (MQ=255)

                                                                            cAAAAGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGAc              >  1:3997410/1‑93 (MQ=255)

                                                                             aaaaGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACa             <  1:5816766/93‑1 (MQ=255)

                                                                               aaGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAAt           <  1:2084016/93‑1 (MQ=255)

                                                                                aGCATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATg          <  1:2284611/93‑1 (MQ=255)

                                                                                 gcgtCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGt         <  2:2381124/90‑1 (MQ=255)

                                                                                  cATCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTc        <  2:4028541/93‑1 (MQ=255)

                                                                                    tCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAg      <  1:2943177/93‑1 (MQ=255)

                                                                                    tCTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAg      <  1:5549197/93‑1 (MQ=255)

                                                                                     cTGCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAgc     <  1:487925/93‑1 (MQ=255)

                                                                                       gCCTC‑TGGG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAgcgc   >  2:2322656/1‑93 (MQ=255)

ACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑T‑GG‑G‑G‑G‑G‑GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCA  >  NC_000913/1207066‑1207245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`