BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,869,856	(G)_6→7	coding (1448/1476 nt)	yeaI →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,869,850	1	.	G	99.5%	658.7 / ‑3.7	189	coding (1442/1476 nt)	yeaI	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (85/103); total (86/103)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.95e-01

Only 100 of 214 total aligned reads displayed.

CAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGA‑‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATTTAT  >  NC_000913/1869758‑1869937

cAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGa                                                                                            >  2:1728117/1‑93 (MQ=255)

  gTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGA‑‑gg                                                                                        <  1:251769/93‑1 (MQ=255)

     ttGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGA‑‑ggggg                                                                                     <  2:2044743/93‑1 (MQ=255)

     ttGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGA‑‑ggggg                                                                                     <  1:2340629/93‑1 (MQ=255)

      tGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGA‑‑gggggg                                                                                    >  2:4060605/1‑93 (MQ=255)

          aCGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGA‑‑gggggggcaa                                                                                >  1:2061074/1‑89 (MQ=255)

             gTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑caacaa                                                                             <  1:1689293/93‑1 (MQ=255)

                ttttCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGt                                                                          <  2:2418271/93‑1 (MQ=255)

                 tttCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTg                                                                         >  1:1198202/1‑93 (MQ=255)

                   tCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGat                                                                       <  2:898136/93‑1 (MQ=255)

                   tCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGat                                                                       <  1:2914912/93‑1 (MQ=255)

                    cAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGatt                                                                      <  1:1085523/93‑1 (MQ=255)

                     aaCGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGatta                                                                     <  2:2585366/93‑1 (MQ=255)

                      aCGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGAGattat                                                                    >  2:710806/1‑93 (MQ=255)

                         ggTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATtca                                                                 <  2:6133614/93‑1 (MQ=255)

                         ggTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATtca                                                                 >  1:1949529/1‑93 (MQ=255)

                         ggTGACAAGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATtca                                                                 <  2:3905134/93‑1 (MQ=255)

                          gTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGAGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATtcat                                                                <  1:5241731/93‑1 (MQ=255)

                           tGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATtcatc                                                               <  2:637604/93‑1 (MQ=255)

                               acGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCatat                                                           <  1:228200/93‑1 (MQ=255)

                               acGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCatat                                                           >  1:4823710/1‑93 (MQ=255)

                               acGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCatat                                                           <  1:3657134/93‑1 (MQ=255)

                                cgcgTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATAtt                                                          <  1:4130461/93‑1 (MQ=255)

                                 gcgTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATAttt                                                         <  1:971933/93‑1 (MQ=255)

                                 gcgTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATAttt                                                         >  1:6466525/1‑93 (MQ=255)

                                  cgTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTa                                                        <  1:5753713/93‑1 (MQ=255)

                                     aaTATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGt                                                     <  1:4283108/93‑1 (MQ=255)

                                     aaTATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGt                                                     >  2:5063532/1‑93 (MQ=255)

                                     aaTATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGt                                                     <  1:1416967/93‑1 (MQ=255)

                                     aaTATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGt                                                     >  1:5932189/1‑93 (MQ=255)

                                     aaTATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGt                                                     <  1:2630718/93‑1 (MQ=255)

                                     aaTATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGt                                                     <  1:2793128/93‑1 (MQ=255)

                                      atatCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTg                                                    >  2:5288880/1‑93 (MQ=255)

                                      atatCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCCAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTg                                                    <  2:622827/93‑1 (MQ=255)

                                       tatCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGc                                                   <  1:2988782/93‑1 (MQ=255)

                                         tctcGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCaa                                                 >  1:5286645/1‑93 (MQ=255)

                                              cTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAata                                            <  1:3244797/93‑1 (MQ=255)

                                               ttGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAatat                                           >  2:3999788/1‑93 (MQ=255)

                                               ttGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAatat                                           >  1:4868631/1‑93 (MQ=255)

                                                tGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATAtt                                          >  1:4740816/1‑93 (MQ=255)

                                                  tCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTc                                         <  1:2122270/93‑1 (MQ=255)

                                                  tCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCa                                        <  1:5351401/93‑1 (MQ=255)

                                                  tCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCa                                        >  2:4514215/1‑93 (MQ=255)

                                                  tCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATACTTAAGTGCAAAAATATTCa                                        <  1:6239408/93‑1 (MQ=255)

                                                   cATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCag                                       >  1:1001798/1‑93 (MQ=255)

                                                   cATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCag                                       >  1:823625/1‑93 (MQ=255)

                                                   cATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCag                                       <  2:5598306/93‑1 (MQ=255)

                                                    aTGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCaga                                      <  2:4134132/93‑1 (MQ=255)

                                                      taCGGATGCAGATAAAGCCGTACGCGAAGCGAAACGCGA‑‑GGGGGGCCAACATAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGc                                    <  1:4946433/92‑1 (MQ=255)

                                                      gACGGAAGCAGATAAAGCCTTACGCGACGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGc                                    <  1:2458706/93‑1 (MQ=255)

                                                       aCGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGcc                                   <  1:2371397/93‑1 (MQ=255)

                                                        cGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCa                                  >  1:4442657/1‑93 (MQ=255)

                                                         ggAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCAt                                 <  2:4200100/93‑1 (MQ=255)

                                                         ggAAGCAGATAAAGCCTTAAGCGAAGCGAAAAGCGAG‑GGGGGG‑GAACAAAGTGATTATTCATCATGTTTAAGTGCACATATATTCAGAGCCAt                                 >  1:4669154/1‑93 (MQ=255)

                                                          gAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATg                                >  1:3854561/1‑93 (MQ=255)

                                                          gAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATg                                >  1:5210533/1‑93 (MQ=255)

                                                          gAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATg                                >  2:1796079/1‑93 (MQ=255)

                                                           aaGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGc                               <  1:2222174/93‑1 (MQ=255)

                                                           aaGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGc                               <  1:4566894/93‑1 (MQ=255)

                                                            aGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCt                              <  2:3886144/93‑1 (MQ=255)

                                                             gCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCtt                             >  2:1642659/1‑93 (MQ=255)

                                                             gCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCtt                             <  1:6190452/93‑1 (MQ=255)

                                                             gCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCtt                             <  2:4867788/93‑1 (MQ=255)

                                                              cAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCttt                            >  2:5987598/1‑93 (MQ=255)

                                                               aGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCtttt                           <  2:2874581/93‑1 (MQ=255)

                                                               aGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCtttt                           >  1:5586685/1‑93 (MQ=255)

                                                               aGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCtttt                           >  2:115378/1‑93 (MQ=255)

                                                                gATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCttttt                          <  2:5495693/93‑1 (MQ=255)

                                                                  tAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGc                        <  2:5327510/93‑1 (MQ=255)

                                                                  tAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGc                        <  1:1813488/93‑1 (MQ=255)

                                                                   aaaGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCg                       <  2:3582025/93‑1 (MQ=255)

                                                                    aaGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGt                      <  2:1863651/93‑1 (MQ=255)

                                                                     aGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTg                     >  2:3019042/1‑93 (MQ=255)

                                                                     aGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTg                     <  1:5046813/93‑1 (MQ=255)

                                                                      gCCTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTgg                    >  2:2348589/1‑93 (MQ=255)

                                                                       ccTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGc                   <  1:3218540/93‑1 (MQ=255)

                                                                       ccTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGc                   <  1:1796972/93‑1 (MQ=255)

                                                                       ccTTACGCGAAGCAAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGc                   <  2:2155507/93‑1 (MQ=255)

                                                                        cTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCt                  <  1:3747876/93‑1 (MQ=255)

                                                                        cTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCt                  >  1:3558245/1‑93 (MQ=255)

                                                                        cTTACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCt                  >  2:877791/1‑93 (MQ=255)

                                                                          tACGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCttt                <  2:846884/93‑1 (MQ=255)

                                                                           aCGCGAAGCGAAAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCtttt               >  2:5023960/1‑93 (MQ=255)

                                                                             gcgaagcgaaAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTga             >  2:1468314/1‑92 (MQ=255)

                                                                              cgaagcgaaAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCa            >  2:1007224/1‑93 (MQ=255)

                                                                               gaagcgaaAAGCGAGGGGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCa            >  1:715783/1‑93 (MQ=255)

                                                                               gaagcgaaAAGCGAG‑GGGGGG‑CAACAAAGTGATTACTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGGGTGGCTTTTGCAt           >  2:1401071/1‑93 (MQ=255)

                                                                               gaagcgaaAAGCGA‑‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATa          >  2:5590928/1‑93 (MQ=255)

                                                                                aagcgaaAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATa          <  1:1382921/93‑1 (MQ=255)

                                                                                aagcgaaAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATa          <  2:5249260/93‑1 (MQ=255)

                                                                                 agcgaaAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATAc         <  2:543255/93‑1 (MQ=255)

                                                                                  gcgaaAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACa        <  2:4578148/93‑1 (MQ=255)

                                                                                  gcgaaAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACa        <  2:3517698/93‑1 (MQ=255)

                                                                                   cgaaAAGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACaa       <  1:6001649/93‑1 (MQ=255)

                                                                                     aaaaGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAAtt     >  2:281818/1‑93 (MQ=255)

                                                                                     aaaaGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAAtt     <  1:2698810/93‑1 (MQ=255)

                                                                                      aaaGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAAttt    <  2:3240639/93‑1 (MQ=255)

                                                                                       aaGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATtta   >  2:2120758/1‑93 (MQ=255)

                                                                                        aGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATttat  >  1:3608181/1‑93 (MQ=255)

                                                                                        aGCGAG‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATttat  <  1:4076082/93‑1 (MQ=255)

CAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGA‑‑GGGGGG‑CAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATTTAT  >  NC_000913/1869758‑1869937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`