BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,265,000	(G)_5→6	coding (736/1467 nt)	yeiQ →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,264,995	1	.	G	99.1%	767.2 / ‑1.6	220	coding (731/1467 nt)	yeiQ	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (100/118); total (100/120)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 254 total aligned reads displayed.

GATAAAAGAGCACGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCT‑GGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTG  >  NC_000913/2264903‑2265082

 aTAAAAGAGCACGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCT‑g                                                                                        >  1:801719/1‑93 (MQ=255)

  tAAAAGAGCACGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCT‑gg                                                                                       <  1:3567405/93‑1 (MQ=255)

  tAAAAGAGCACGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCT‑gg                                                                                       >  1:4948164/1‑93 (MQ=255)

   aaaaGAGCACGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCT‑ggg                                                                                      <  1:4115950/93‑1 (MQ=255)

   aaaaGAGCACGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCT‑ggg                                                                                      <  2:2861828/93‑1 (MQ=255)

     aaGAGCACGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGTCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCT‑ggggg                                                                                    >  2:5555762/1‑93 (MQ=255)

     aaGAGCACGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCT‑ggggg                                                                                    >  2:3360619/1‑93 (MQ=255)

      agagCACGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCT‑gggggg                                                                                   >  1:5286035/1‑92 (MQ=255)

      agagCACGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCT‑gggggg                                                                                   <  2:6106041/93‑2 (MQ=255)

      agagCACGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCT‑gggggg                                                                                   <  2:4646318/93‑2 (MQ=255)

        agCACGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCT‑ggggggtg                                                                                 <  1:5337896/93‑4 (MQ=255)

           aCGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAAt                                                                               <  1:1674112/93‑1 (MQ=255)

           aCGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAAt                                                                               >  2:233059/1‑93 (MQ=255)

            cGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATg                                                                              >  1:3707283/1‑93 (MQ=255)

              tCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGAt                                                                            >  2:1011385/1‑93 (MQ=255)

              tCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGAt                                                                            >  2:1345252/1‑93 (MQ=255)

                aGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATcc                                                                          >  1:5891462/1‑93 (MQ=255)

                aGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATcc                                                                          <  2:473915/93‑1 (MQ=255)

                 gTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATccc                                                                         <  2:3855726/93‑1 (MQ=255)

                 gTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATccc                                                                         <  2:3501508/93‑1 (MQ=255)

                  ttttCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCt                                                                        <  1:1738233/93‑1 (MQ=255)

                  ttttCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCt                                                                        <  2:4768439/93‑1 (MQ=255)

                    ttCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTgc                                                                      <  2:5878720/93‑1 (MQ=255)

                         ggAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGAt                                                                 <  1:4872983/93‑1 (MQ=255)

                             ccATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGc                                                             <  2:1695635/93‑1 (MQ=255)

                             ccATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGc                                                             <  1:6164327/93‑1 (MQ=255)

                             ccATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGc                                                             <  1:3746179/93‑1 (MQ=255)

                              cATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGCGAATGATCCCTGCGCGATTAGCt                                                            >  2:2585128/1‑93 (MQ=255)

                              cATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGCAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCt                                                            <  1:5184984/93‑1 (MQ=255)

                               ctGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTg                                                           <  2:6249896/92‑1 (MQ=255)

                                tGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGc                                                          >  1:4649623/1‑93 (MQ=255)

                                tGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGc                                                          <  1:4999010/93‑1 (MQ=255)

                                 ggTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCg                                                         <  1:4233370/93‑1 (MQ=255)

                                    cGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAAc                                                      <  2:2089193/93‑1 (MQ=255)

                                    cGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAAc                                                      <  2:915526/93‑1 (MQ=255)

                                     gACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAAcc                                                     <  2:1218593/93‑1 (MQ=255)

                                     gACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAAcc                                                     >  2:1059660/1‑93 (MQ=255)

                                      aCCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCg                                                    >  1:5482733/1‑93 (MQ=255)

                                         gCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGttt                                                 <  2:686109/93‑1 (MQ=255)

                                         gCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGttt                                                 <  2:5914857/93‑1 (MQ=255)

                                          cATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTa                                                <  1:2975903/93‑1 (MQ=255)

                                          cATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTa                                                <  2:5963208/93‑1 (MQ=255)

                                            ttGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGACCCGCTTATc                                              >  1:1153008/1‑93 (MQ=255)

                                             tGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATcc                                             <  1:4291487/93‑1 (MQ=255)

                                             tGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATcc                                             >  2:2890204/1‑93 (MQ=255)

                                              gtcccGCCTGCAACCGACGCATCACTGGTGGAATTCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCa                                            <  1:5726009/90‑1 (MQ=255)

                                               ttCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAg                                           <  1:5824098/93‑1 (MQ=255)

                                                  cGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtgg                                        <  2:769336/93‑1 (MQ=255)

                                                   ggCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtggg                                       <  1:5194371/93‑1 (MQ=255)

                                                     cTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtgggtg                                     <  1:941169/93‑1 (MQ=255)

                                                     cTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtgggtg                                     >  1:2747995/1‑93 (MQ=255)

                                                       gCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtgggtggg                                   >  2:1446856/1‑92 (MQ=255)

                                                       gCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGggtggt                                   >  2:6336410/1‑93 (MQ=255)

                                                       gCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGggtggt                                   <  2:4130746/93‑1 (MQ=255)

                                                        aaaCCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGgtggtg                                  >  1:2521526/2‑93 (MQ=255)

                                                         aaCCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGtggtgg                                 >  1:4477848/1‑93 (MQ=255)

                                                            cgacgaATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAg                              <  2:5707001/93‑1 (MQ=255)

                                                             gacgaATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGa                             <  2:1081545/93‑1 (MQ=255)

                                                             gacgaATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGa                             >  1:3831149/1‑93 (MQ=255)

                                                                 aaTCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAAc                         >  1:1825902/1‑93 (MQ=255)

                                                                 aaTCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAAc                         >  2:4012966/1‑93 (MQ=255)

                                                                 aaTCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAAc                         >  2:3231078/1‑93 (MQ=255)

                                                                 aaTCACTGGTGGAAATCAGCCAGCATCTGGGGGGGGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATaat                         >  2:1535170/1‑92 (MQ=255)

                                                                  aTCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACt                        <  2:2416083/93‑1 (MQ=255)

                                                                  aTCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACt                        >  1:2467327/1‑93 (MQ=255)

                                                                   tCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACtt                       >  1:2247597/1‑93 (MQ=255)

                                                                   tCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACtt                       >  2:419075/1‑93 (MQ=255)

                                                                    cACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtc                      <  1:230348/93‑1 (MQ=255)

                                                                     aCTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcg                     >  1:4389836/1‑93 (MQ=255)

                                                                     aCTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcg                     >  1:6264115/1‑93 (MQ=255)

                                                                     aCTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCCGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcg                     <  2:4859938/93‑1 (MQ=255)

                                                                      cTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcgt                    <  2:3804866/93‑1 (MQ=255)

                                                                       tggtggAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcgtc                   >  1:1016602/1‑93 (MQ=255)

                                                                       tggtggAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcgtc                   >  1:175062/1‑93 (MQ=255)

                                                                       tggtggAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcgtc                   <  1:3884803/93‑1 (MQ=255)

                                                                       tggtggAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcgtc                   <  2:1394168/93‑1 (MQ=255)

                                                                        ggtggAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcgtcg                  <  1:5673550/93‑1 (MQ=255)

                                                                        ggtggAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcgtcg                  >  1:53112/1‑93 (MQ=255)

                                                                         gtggAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGc                 <  2:1301204/93‑1 (MQ=255)

                                                                          tggAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACGGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCt                <  2:1952160/93‑1 (MQ=255)

                                                                           ggAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTg               <  1:4077140/93‑1 (MQ=255)

                                                                             aaaTCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTggg             >  2:5016027/1‑93 (MQ=255)

                                                                              aaTCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGc            >  2:2133943/1‑93 (MQ=255)

                                                                               aTCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCg           >  2:3773298/1‑93 (MQ=255)

                                                                               aTCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCg           >  1:325457/1‑93 (MQ=255)

                                                                               aTCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCg           >  1:6438881/1‑93 (MQ=255)

                                                                                tCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGt          <  2:1265864/93‑1 (MQ=255)

                                                                                tCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGt          >  1:3052359/1‑93 (MQ=255)

                                                                                tCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGt          <  1:127560/93‑1 (MQ=255)

                                                                                  agccagcATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcc        <  2:4574938/93‑1 (MQ=255)

                                                                                  agccagcATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcc        <  1:3606621/93‑1 (MQ=255)

                                                                                   gccagcATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcct       >  1:5511087/1‑93 (MQ=255)

                                                                                    ccagcATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcctg      <  1:5009360/93‑1 (MQ=255)

                                                                                     cagcATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcctgc     >  2:5113711/1‑93 (MQ=255)

                                                                                     cagcATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcctgc     >  1:214635/1‑93 (MQ=255)

                                                                                      agcATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcctgcc    >  2:4438286/1‑93 (MQ=255)

                                                                                      agcATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcctgcc    <  1:6025677/93‑1 (MQ=255)

                                                                                      agcATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcctgcc    <  2:3442783/93‑1 (MQ=255)

                                                                                       gcATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcctgcct   >  1:2606857/1‑93 (MQ=255)

                                                                                        cATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcctgcctg  <  2:264470/93‑1 (MQ=255)

GATAAAAGAGCACGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCT‑GGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTG  >  NC_000913/2264903‑2265082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`