BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,075,220	(T)_5→6	coding (256/357 nt)	yggP ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,075,215	1	.	T	98.9%	694.8 / ‑0.7	178	coding (261/357 nt)	yggP	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/2); new base T (97/79); total (97/81)
Fisher's exact test for biased strand distribution p-value = 2.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 220 total aligned reads displayed.

ATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAA  >  NC_000913/3075123‑3075302

ataAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGcc                                                                                           >  2:2544508/1‑93 (MQ=255)

ataAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGcc                                                                                           <  2:5233105/93‑1 (MQ=255)

ataAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGcc                                                                                           <  2:4777126/93‑1 (MQ=255)

 taAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑t                                                                                         >  1:2458081/1‑93 (MQ=255)

 taAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑t                                                                                         >  2:4336382/1‑93 (MQ=255)

  aaGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑tt                                                                                        <  1:1415615/93‑1 (MQ=255)

   aGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑tt‑t                                                                                      >  2:729421/1‑93 (MQ=255)

   aGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑tt‑t                                                                                      <  2:322842/93‑1 (MQ=255)

   aGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑tt‑t                                                                                      <  1:122604/93‑1 (MQ=255)

    gTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑tt‑tt                                                                                     <  2:5668436/93‑1 (MQ=255)

    gTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑tt‑tt                                                                                     >  2:3018557/1‑93 (MQ=255)

     tGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑tt‑ttt                                                                                    >  2:331592/1‑93 (MQ=255)

     tGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑tt‑ttt                                                                                    <  2:1475961/93‑1 (MQ=255)

      ggCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑tt‑tttt                                                                                   >  1:5485239/1‑92 (MQ=255)

       gCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑tt‑tttta                                                                                  <  2:2702822/93‑3 (MQ=255)

       gCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑tt‑tttta                                                                                  >  1:2472939/1‑91 (MQ=255)

       gCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑tt‑tttta                                                                                  <  2:1533578/93‑3 (MQ=255)

       gCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑tt‑tttta                                                                                  >  1:4049982/1‑91 (MQ=255)

         aTTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATTTCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑tt‑ttttata                                                                                <  1:939377/93‑5 (MQ=255)

         aTTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑tt‑ttttata                                                                                >  1:3831754/1‑89 (MQ=255)

         aTTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑tt‑ttttata                                                                                >  2:1182041/1‑89 (MQ=255)

         aTTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑tt‑ttttata                                                                                <  1:2260749/93‑5 (MQ=255)

           tGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATAtt                                                                               <  2:5152985/93‑1 (MQ=255)

           tGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATAtt                                                                               <  1:69178/93‑1 (MQ=255)

            gccgccAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGGCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATAttt                                                                              >  2:5952134/1‑93 (MQ=255)

            gccgccAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATAttt                                                                              <  1:3459585/93‑1 (MQ=255)

             ccgccAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTg                                                                             <  1:5338201/93‑1 (MQ=255)

               gccAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGcc                                                                           >  2:4382132/1‑93 (MQ=255)

               gccAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAAAGCTGGCCTTT‑TTTATATTTccc                                                                           >  1:1114776/1‑93 (MQ=255)

                ccAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCa                                                                          >  1:3964569/1‑93 (MQ=255)

                ccAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCa                                                                          <  1:3702807/93‑1 (MQ=255)

                    aaTATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTga                                                                      <  2:4498934/93‑1 (MQ=255)

                     atagTCGTATCTGTAGCCCGCTGAATCTCCTCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTgag                                                                     <  1:2089324/93‑1 (MQ=255)

                       atTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGAt                                                                   <  1:4397340/93‑1 (MQ=255)

                        ttCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGAtt                                                                  >  2:3978425/1‑93 (MQ=255)

                           gTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGAttttt                                                               >  1:565870/1‑93 (MQ=255)

                           gTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGAttttt                                                               <  2:6287984/93‑1 (MQ=255)

                             aGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTAc                                                             <  1:1786874/93‑1 (MQ=255)

                             aGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTAc                                                             >  1:4674834/1‑93 (MQ=255)

                             aGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTAc                                                             >  1:3508094/1‑93 (MQ=255)

                              gCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTAcc                                                            <  1:594335/93‑1 (MQ=255)

                                tGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCa                                                          >  1:4598987/1‑93 (MQ=255)

                                 gTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCAc                                                         >  1:884908/1‑93 (MQ=255)

                                 gTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCAc                                                         >  1:2026048/1‑93 (MQ=255)

                                   aGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTATCCAcct                                                       >  2:4947270/1‑91 (MQ=255)

                                   aGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACtt                                                       >  2:1134254/1‑93 (MQ=255)

                                    gCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTc                                                      >  1:3238322/1‑93 (MQ=255)

                                    gCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTc                                                      <  2:1423965/93‑1 (MQ=255)

                                    gCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTc                                                      <  2:3839139/93‑1 (MQ=255)

                                      ccGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGa                                                    <  1:5786768/93‑1 (MQ=255)

                                      ccGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGa                                                    >  2:2644056/1‑93 (MQ=255)

                                       cGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGAc                                                   <  2:3393262/93‑1 (MQ=255)

                                       cGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGAc                                                   >  2:6422218/1‑93 (MQ=255)

                                       cGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGAc                                                   >  1:5466165/1‑93 (MQ=255)

                                        gCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACa                                                  >  2:513556/1‑93 (MQ=255)

                                        gCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACa                                                  >  1:3370187/1‑93 (MQ=255)

                                          tGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAAt                                                >  1:1640284/1‑93 (MQ=255)

                                             atatCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTCTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAAc                                             <  2:3438584/93‑1 (MQ=255)

                                              tatCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAAcc                                            <  2:5830069/93‑1 (MQ=255)

                                               atCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAAccc                                           <  2:5463315/93‑1 (MQ=255)

                                               atCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAAccc                                           <  1:4910189/93‑1 (MQ=255)

                                                tCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAAcccc                                          >  2:2186485/1‑93 (MQ=255)

                                                tCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAAcccc                                          <  2:4426241/93‑1 (MQ=255)

                                                tCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAAccca                                          >  2:5078612/1‑92 (MQ=255)

                                                 ccGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCg                                         >  2:1610654/1‑93 (MQ=255)

                                                   gCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGc                                       <  1:1338397/93‑1 (MQ=255)

                                                    cTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGca                                      >  2:1021981/1‑93 (MQ=255)

                                                      tGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGcaca                                    <  1:5032165/93‑1 (MQ=255)

                                                      tGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGcaca                                    >  2:1668851/1‑93 (MQ=255)

                                                       ggTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACAt                                   <  2:6444795/93‑1 (MQ=255)

                                                       ggTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACAt                                   >  2:1079312/1‑93 (MQ=255)

                                                          aaCCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCa                                <  1:4242183/93‑1 (MQ=255)

                                                           aCCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCAt                               >  2:1837017/1‑93 (MQ=255)

                                                            ccccATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATg                              >  1:6387341/1‑93 (MQ=255)

                                                            ccccATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATg                              >  2:1433991/1‑93 (MQ=255)

                                                              ccATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGAGAATAACCCCGGCACATTCATGcc                            <  1:3676499/93‑1 (MQ=255)

                                                              ccATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGcc                            <  2:636962/93‑1 (MQ=255)

                                                              ccATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGcc                            >  2:2006706/1‑93 (MQ=255)

                                                              ccATCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGcc                            <  2:2311615/93‑1 (MQ=255)

                                                                aTCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCg                          >  1:6214861/1‑93 (MQ=255)

                                                                 tCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCgg                         >  2:3573972/1‑93 (MQ=255)

                                                                 tCGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCgg                         >  1:5269120/1‑93 (MQ=255)

                                                                  cGCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGt                        <  1:6206309/93‑1 (MQ=255)

                                                                   gCCGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTa                       <  1:3005063/93‑1 (MQ=255)

                                                                    ccGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTaa                      <  1:4879055/93‑1 (MQ=255)

                                                                     cGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAAt                     <  1:1556653/93‑1 (MQ=255)

                                                                     cGGTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAAt                     >  1:646526/1‑93 (MQ=255)

                                                                      ggTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATg                    >  1:4464137/1‑93 (MQ=255)

                                                                      ggTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATg                    >  1:852777/1‑93 (MQ=255)

                                                                       gTTGCAATACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGa                   >  1:237304/1‑93 (MQ=255)

                                                                            aaTACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATACCCCCGGCACATTCATGCCCGGTAATGACCGga              <  2:2387641/93‑1 (MQ=255)

                                                                            aaTACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGga              <  2:1754978/93‑1 (MQ=255)

                                                                              tACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGgatg            >  1:1063107/1‑93 (MQ=255)

                                                                              tACAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGgatg            <  2:4145269/93‑1 (MQ=255)

                                                                                cAAAACGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGgatgat          <  1:4498151/93‑1 (MQ=255)

                                                                                  aaaCGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGAttt        <  2:3202433/93‑1 (MQ=255)

                                                                                    aCGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTc      >  1:3202747/1‑93 (MQ=255)

                                                                                     cGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCt     <  1:4945176/93‑1 (MQ=255)

                                                                                     cGCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCt     <  2:3167155/93‑1 (MQ=255)

                                                                                      gCTGGCCTTT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTa    >  1:4326217/1‑93 (MQ=255)

ATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TT‑TTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAA  >  NC_000913/3075123‑3075302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`