BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,725,747	(C)_8→9	coding (1254/1455 nt)	xylB ←	xylulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,725,739	1	.	C	94.7%	442.3 / 14.6	133	coding (1262/1455 nt)	xylB	xylulokinase
Reads supporting (aligned to +/- strand): ref base . (3/4); new base C (78/48); total (81/52)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 166 total aligned reads displayed.

CTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑AT‑‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATC  >  NC_000913/3725647‑3725825

cTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑At                                                                                           >  2:646752/1‑93 (MQ=255)

 tAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑AT‑‑c                                                                                        >  2:314751/1‑93 (MQ=255)

 tAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑AT‑‑c                                                                                        >  1:2147408/1‑93 (MQ=255)

   aCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑AT‑‑ccc                                                                                      <  2:117882/93‑1 (MQ=255)

   aCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑AT‑‑ccc                                                                                      >  1:4111972/1‑93 (MQ=255)

      gTAGTTGCGGCAACAATTCCATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑AT‑‑cccccc                                                                                   >  1:6310737/1‑93 (MQ=255)

       tAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑AT‑‑ccccccc                                                                                  >  2:2978996/1‑93 (MQ=255)

       tAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑AT‑‑ccccccc                                                                                  >  1:2284245/1‑93 (MQ=255)

        aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑AT‑‑cccccccc                                                                                 >  2:5228381/1‑93 (MQ=255)

         gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑AT‑‑ccccccccc                                                                                <  2:1360879/93‑2 (MQ=255)

          ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑AT‑‑cccccccccg                                                                               <  2:4122543/93‑3 (MQ=255)

          ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑AT‑‑cccccccccc                                                                               >  2:3708140/1‑91 (MQ=255)

             cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑AT‑‑CCCCCCCCGTAC‑g                                                                           <  2:2057022/93‑1 (MQ=255)

              ggCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑g                                                                           <  1:5847068/93‑1 (MQ=255)

                caacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGt                                                                         >  2:1973469/1‑93 (MQ=255)

                   caaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAAt                                                                      >  2:5621337/1‑93 (MQ=255)

                     aTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCCTGC‑GGTAATCg                                                                    >  1:259084/1‑93 (MQ=255)

                       tCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAg                                                                  <  2:5038296/93‑1 (MQ=255)

                        cAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAgc                                                                 <  2:4925786/93‑1 (MQ=255)

                         aaTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAgct                                                                >  1:1975546/1‑93 (MQ=255)

                          aTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAgctg                                                               >  1:5774174/1‑93 (MQ=255)

                          aTGAGCGAATTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAgctg                                                               >  1:6298327/1‑93 (MQ=255)

                           tGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAgctgc                                                              <  1:4239050/93‑1 (MQ=255)

                           tGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAgctgc                                                              >  1:3114847/1‑93 (MQ=255)

                              gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGa                                                           <  1:3655270/93‑1 (MQ=255)

                               cGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGAc                                                          <  2:171608/93‑1 (MQ=255)

                               cGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGAc                                                          <  2:972358/93‑1 (MQ=255)

                               cGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCCGAC‑GGTAATCGAGCTTGTGAc                                                          >  2:2120015/1‑93 (MQ=255)

                               cGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCACCCAGTGCTGGGCCCAC‑ATA‑CCCCCCCCGTGC‑TGTAGTCGTGcggctggc                                                          >  2:3433897/1‑91 (MQ=255)

                                gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGAcc                                                         >  1:1083577/1‑93 (MQ=255)

                                 aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCg                                                        <  1:992791/93‑1 (MQ=255)

                                 aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCg                                                        >  2:3994234/1‑93 (MQ=255)

                                 aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCg                                                        >  2:901977/1‑93 (MQ=255)

                                 aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGGAATGGAGCTGCTGAGCg                                                        >  1:2854878/1‑93 (MQ=255)

                                   ttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTCATCGAGCTGCTGACCGCt                                                      >  2:1498014/1‑93 (MQ=255)

                                    tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTg                                                     >  1:2921245/1‑93 (MQ=255)

                                    tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCCTAC‑GGTAATCCAGCTGCTGGCCggcg                                                     >  2:5428815/1‑90 (MQ=255)

                                    tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑AT‑‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGa                                                    <  1:2485521/93‑1 (MQ=255)

                                    tctcTGGATTCGCAGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATCCCCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCt                                                      <  2:4787532/93‑1 (MQ=255)

                                     ctctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGa                                                    <  2:1801356/93‑1 (MQ=255)

                                      tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGat                                                   <  1:5315136/93‑1 (MQ=255)

                                       ctGTATTCCCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGata                                                  <  2:3826686/93‑1 (MQ=255)

                                       ctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGata                                                  <  2:5335439/93‑1 (MQ=255)

                                        tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGatat                                                 <  1:5856964/93‑1 (MQ=255)

                                         ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATc                                                >  2:761449/1‑93 (MQ=255)

                                         ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATc                                                >  1:3267826/1‑93 (MQ=255)

                                         ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑CGTAATCGAGCTGCTGACCGCCGATATc                                                >  1:3924395/1‑93 (MQ=255)

                                          gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATcc                                               <  2:1545478/93‑1 (MQ=255)

                                          gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGGAAACGAGCTGCTGACCGCTGACATcc                                               >  2:3568290/1‑93 (MQ=255)

                                           aTTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATCCCCCCCCCCCTAC‑GGTAATCCAGCTGCTGACCGCTGACATcc                                               >  2:1536996/1‑93 (MQ=255)

                                             tccccgcGATCTCCGCCAGCCTTGCTGCGCACAGTGCTGGCCCCCC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGcc                                            <  1:1266900/90‑1 (MQ=255)

                                             tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGcc                                            <  2:2441970/93‑1 (MQ=255)

                                               gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGGAATTGAGGTGCTGAGCGCTGATATACGcctg                                          >  1:5922272/1‑91 (MQ=255)

                                               gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCCGAC‑GGGAATCGAGCTGCTGACCGCTGATATCCGCCAg                                          >  1:5580591/1‑93 (MQ=255)

                                               cgcgcgAGCTCCGCCCGCCTTTGTGCGCCCAGGGGTTGCCCCCC‑CTCCCCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCa                                           <  2:6442722/91‑1 (MQ=255)

                                                ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGGCCGCTGATATCCGCCAGc                                         >  2:206225/1‑93 (MQ=255)

                                                ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGc                                         >  1:1953811/1‑93 (MQ=255)

                                                ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGc                                         >  1:5074770/1‑93 (MQ=255)

                                                ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGc                                         >  1:3002977/1‑93 (MQ=255)

                                                ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCCGAC‑GGGAATCGAGCTGCTGACCGCTGATATACGCCAGc                                         >  1:1557184/1‑93 (MQ=255)

                                                 cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCa                                        <  2:2744463/93‑1 (MQ=255)

                                                 cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCa                                        <  1:5767706/93‑1 (MQ=255)

                                                  gcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCCGCAt                                       >  1:5665208/1‑93 (MQ=255)

                                                  gcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGGAt                                       >  2:2513516/1‑93 (MQ=255)

                                                  gcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAt                                       >  2:4867314/1‑93 (MQ=255)

                                                  gcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAt                                       <  2:4432859/93‑1 (MQ=255)

                                                  gcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCCGAC‑GGGGATCGAGCTTCTGACCGCGGATATCCGCGAGCac                                       >  1:4769972/1‑92 (MQ=255)

                                                    gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                     >  2:6336023/1‑93 (MQ=255)

                                                    gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                     >  2:2505887/1‑93 (MQ=255)

                                                    gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCCTAC‑GGGAATCGAGCCGCTGCACGCTGATATCCGCCAGCGTCt                                     >  2:468756/1‑93 (MQ=255)

                                                     aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTg                                    <  2:2921245/93‑1 (MQ=255)

                                                       cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGAc                                  >  2:3769379/1‑93 (MQ=255)

                                                           gcCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCCTCTGACGCCa                              >  1:732875/1‑93 (MQ=255)

                                                           gcCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa                              >  1:4980284/1‑93 (MQ=255)

                                                           gcCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGCAATCGAGCTGCTGACCGCTGATATTCGCCAGCATCTTACGccc                              >  2:5753800/1‑92 (MQ=255)

                                                            ccAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAg                             <  2:5525970/93‑1 (MQ=255)

                                                               gCCTTGCTGCGCCCAGTGCTGGCCCCCC‑ATC‑CCCCCCCCCTAC‑GGTACTCGCGCTGCTGACCGCTGATATCCGTCTGCATCTGACGCCAGTAc                          >  1:1790624/1‑93 (MQ=255)

                                                               gCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTAc                          <  2:5355523/93‑1 (MQ=255)

                                                               gCCTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGACAGCATCTGACGCCCGTAc                          >  1:3153124/1‑93 (MQ=255)

                                                                 cTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTc                        >  2:4858536/1‑93 (MQ=255)

                                                                 cTTGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTc                        <  2:5539745/93‑1 (MQ=255)

                                                                  ttGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCa                       <  2:3489670/93‑1 (MQ=255)

                                                                  ttGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGCAATTAAGCTGCTAACCCCTGATATTCGCCCGCCACTGACGCCCATACTCa                       >  2:369957/1‑93 (MQ=255)

                                                                  ttGCTGCGCCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCCTAC‑GGTAATAGAGGTGCTGAACCCCGCTATCCGCCACCCACTTGCGCCGGTACTcc                       >  1:274695/1‑92 (MQ=255)

                                                                  ttGCTGCGCCCAGTGCTGGCCCCAC‑AT‑‑CCCCCCCCCGACGGGAAATGAAGCTCTGGACGGCGGAAATCCGCCAGCATCTACCGCCAGTACTCa                       >  2:2939101/1‑93 (MQ=255)

                                                                   tgctgcGCCCAGTGCTGTCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCAc                      <  2:3832442/93‑1 (MQ=255)

                                                                      tgcGCCCAGTGGTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAc                   <  2:2157016/93‑1 (MQ=255)

                                                                       gcccccAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAcg                  <  2:3145271/90‑1 (MQ=255)

                                                                        cgcCCAGTGCTGGCCCCACAAC‑‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAcgc                 <  2:187717/93‑1 (MQ=255)

                                                                        cgcCCAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAcgc                 <  2:924879/93‑1 (MQ=255)

                                                                          cccAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACgcgc               <  2:5675839/93‑1 (MQ=255)

                                                                           ccAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcc              <  1:4037849/93‑1 (MQ=255)

                                                                           ccAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCCGACCGCTGGTTTCCGCCAGCATCTGACGCCAGTACTCACTAcgcggc              >  1:6061308/1‑91 (MQ=255)

                                                                           ccAGTGCTGCCCCCAC‑AT‑‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc             <  1:4290643/93‑1 (MQ=255)

                                                                            cAGTGCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc             <  2:4974252/93‑1 (MQ=255)

                                                                             cgTGCTGCCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcccc            <  2:3259304/92‑1 (MQ=255)

                                                                               ggCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgc          <  2:4106305/92‑1 (MQ=255)

                                                                                gCTGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgcc         >  2:1535252/1‑93 (MQ=255)

                                                                                  tGGCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCCCTGATATCCGCCAGCATCTGACGCCAGTACTCACTCCGCgccccgcccc       >  2:4247371/1‑93 (MQ=255)

                                                                                    gCCCCAC‑ATC‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCa     <  2:1064923/93‑1 (MQ=255)

CTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑AT‑‑CCCCCCCCGTAC‑GGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATC  >  NC_000913/3725647‑3725825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`