BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,951,166	+C	coding (35/1818 nt)	ilvD →	dihydroxyacid dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,951,166	1	.	C	100.0%	354.5 / NA	103	G12A (GGG→GCG)	ilvD	dihydroxyacid dehydratase
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (16/87); total (16/87)

Only 100 of 132 total aligned reads displayed.

GAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑G‑GC‑C‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAATTTGTACCGGGTCAC  >  NC_000913/3951074‑3951248

gagaCAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑g‑‑g                                                                                         <  2:3367596/93‑1 (MQ=255)

gagaCAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑g‑‑g                                                                                         <  2:4921896/93‑1 (MQ=255)

 agacagacACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑g‑‑gc                                                                                        <  2:3127508/93‑2 (MQ=255)

 agacagacACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑g‑‑gc                                                                                        <  2:6422583/93‑2 (MQ=255)

 agacagacACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGGTCGT‑‑‑GC‑‑GCTGT‑g‑‑g‑g                                                                                       >  2:5252154/1‑93 (MQ=255)

 agacagacACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGGTCGT‑‑‑GC‑‑GCCGT‑g‑‑g‑g                                                                                       >  2:5248651/1‑93 (MQ=255)

 agacagacACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGCTTGT‑‑‑GC‑‑GCCGT‑c‑‑g‑g                                                                                       >  1:5064141/1‑93 (MQ=255)

  gacagacaCTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGGCCGT‑‑‑GC‑‑GGTGT‑g‑‑g‑gg                                                                                      >  2:2837503/1‑92 (MQ=255)

   acagacaCTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGGTCGT‑‑‑GC‑‑GCTGT‑g‑‑g‑ggg                                                                                     >  2:1342078/1‑91 (MQ=255)

             ggAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGaa                                                                          <  1:1255528/93‑1 (MQ=255)

              gAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAAt                                                                         <  2:6042007/93‑1 (MQ=255)

              gAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAAt                                                                         <  1:5409916/93‑1 (MQ=255)

              gAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAAt                                                                         <  2:1550754/93‑1 (MQ=255)

               aGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATg                                                                        <  2:1668052/93‑1 (MQ=255)

                 taaataaaGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGAc                                                                      <  2:2749342/93‑1 (MQ=255)

                 taaataaaGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGAc                                                                      <  2:6361033/93‑1 (MQ=255)

                 taaataaaGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGAc                                                                      <  1:6334628/93‑1 (MQ=255)

                     taaaGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGAc                                                                  <  1:4630400/93‑1 (MQ=255)

                       aaGTATGCCTAAGTCCCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGc                                                                <  1:712699/93‑1 (MQ=255)

                       aaGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGc                                                                <  1:1089835/93‑1 (MQ=255)

                          tATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑a                                                            <  2:6036207/93‑1 (MQ=255)

                          tATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑a                                                            <  2:1648874/93‑1 (MQ=255)

                          tATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑a                                                            <  2:5492572/93‑1 (MQ=255)

                          tATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑a                                                            <  2:231783/93‑1 (MQ=255)

                          tATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑a                                                            <  1:1815652/93‑1 (MQ=255)

                           aTGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑At                                                           <  1:6053930/93‑1 (MQ=255)

                            tGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑Att                                                          <  1:2663149/93‑1 (MQ=255)

                            tGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑Att                                                          <  2:1967951/93‑1 (MQ=255)

                             gCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑Attt                                                         <  2:2845396/93‑1 (MQ=255)

                              ccTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTc                                                        <  2:4022053/93‑1 (MQ=255)

                                tAAGTACCGTTCCGCCACCACCACTCATTGTCGTAATATGGCGGGGGCTCGT‑‑‑GC‑‑GCCGTGG‑‑G‑GCGC‑‑CACCCGGATGAACGACGCCG‑Ctttttg                                                      >  2:5707331/1‑90 (MQ=255)

                                 aaGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGGTCGT‑‑‑GC‑‑GCTGT‑GGCG‑GG‑G‑‑CACCCGCATGACCGACGCCG‑ATTTTGGt                                                     >  1:3605316/1‑93 (MQ=255)

                                  aGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGG‑‑CCCCCGGATGACCGACGCCG‑ACTTCTGTa                                                    >  1:1515528/1‑93 (MQ=255)

                                   gTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTaa                                                   <  1:3034583/93‑1 (MQ=255)

                                   gTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTaa                                                   <  2:3238794/93‑1 (MQ=255)

                                   gTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGGTCGT‑‑‑GG‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGGATGAACGACGCCG‑ACTTCCGtta                                                   >  1:2329895/1‑91 (MQ=255)

                                     aCCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGc                                                 <  2:288984/93‑1 (MQ=255)

                                     aCCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGGTTGT‑‑‑GCGGGGTGG‑G‑‑G‑GG‑C‑‑CCCCGGGGTGACCGACGCCGAATTTGGGTAAg                                                  >  1:3563835/1‑93 (MQ=255)

                                      ccGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGGTCGT‑‑‑GC‑‑GCCGT‑G‑‑GCGCGC‑‑CCCCCGGATGACCGACCCCC‑ATTTTGGTGAgac                                                >  2:3857842/1‑91 (MQ=255)

                                      ccGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGCTCCG‑‑‑TT‑‑GCTGT‑G‑‑G‑GG‑CGGCACCGGGATGACCGACGCGC‑GTTTTGGCAAGcc                                                >  1:830635/1‑93 (MQ=255)

                                        gTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCCCTT‑‑‑GC‑‑GCCGT‑G‑‑GCGCGC‑‑CACCGGCATGAACGACCCCG‑CTTTCGGGAAGGCgg                                              >  2:3006512/1‑92 (MQ=255)

                                        gTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGGTCGT‑‑‑GG‑‑GCTGT‑G‑‑GCGCGC‑‑CACCCGGATGAACGACGCCC‑GGTTCGGGAAGCCGa                                              >  1:2399430/1‑93 (MQ=255)

                                         ttCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGAt                                             <  2:1670740/93‑1 (MQ=255)

                                         ttCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGAt                                             <  2:5390373/93‑1 (MQ=255)

                                         ttCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGGTCCT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGCAAGAGCGACGCCG‑ATTTCGGGAAGCCGAt                                             >  2:2929670/1‑93 (MQ=255)

                                         ttCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGCTCGTGGGGC‑‑GTGGC‑G‑‑G‑GC‑C‑‑CCCCGGAGTGA‑CGACGCCG‑ATTTCTGTAGGCCggt                                             >  1:3015892/1‑91 (MQ=255)

                                         ttCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGCTCCT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGCATGACCGACCCCC‑CATTTGGTAAGGCgct                                             >  2:3637862/1‑91 (MQ=255)

                                            cGCCACCACCACTCATGGACGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTAt                                          <  1:867267/93‑1 (MQ=255)

                                              ccaccaccaCTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑GACCGGAATTACCGACGCCG‑ATTTCGGTAAGCCGATTATcg                                        <  2:743009/93‑1 (MQ=255)

                                                cccaccacTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATcgcg                                      <  2:4792723/92‑1 (MQ=255)

                                                accaccacTCATGGTCGTAATATGGCGGGGGGTCGT‑‑‑GG‑‑GCTGT‑G‑‑G‑GG‑CGGCACCCGGATGACCGACGCCG‑ATTTCGGTAAGCCGATTATtgcg                                      >  2:1543029/1‑93 (MQ=255)

                                                accaccacTCATGGTCGTAATATGGCGGGGGCTCGT‑‑‑GC‑‑GCCGTGG‑‑G‑GCGC‑‑CACCCGGATGAACGACGCCG‑CTTTCGGTGAGCAGACTATcgcg                                      >  2:340427/1‑93 (MQ=255)

                                                accaccacTCATGGTCGTAATATGGCGGGGGCTCCT‑‑‑GG‑‑GCTGTGG‑‑G‑GCGC‑‑CACCCGGATGAACGACGCCG‑CTTTCGGGTAGCCGATTAttccg                                      >  2:2088079/1‑89 (MQ=255)

                                                 ccaccacTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCgg                                     <  1:353489/93‑1 (MQ=255)

                                                  caccacTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGt                                    <  1:5946472/93‑1 (MQ=255)

                                                  caccacTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGt                                    <  1:1860431/93‑1 (MQ=255)

                                                    ccacTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTtg                                  <  2:711626/93‑1 (MQ=255)

                                                     cacTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTtgt                                 <  1:3992105/93‑1 (MQ=255)

                                                     cacTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTtgt                                 <  2:5384111/93‑1 (MQ=255)

                                                     cacTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTtgt                                 <  2:5668471/93‑1 (MQ=255)

                                                       cTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGa                               <  2:1475600/93‑1 (MQ=255)

                                                         cATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAAc                             <  2:1964700/93‑1 (MQ=255)

                                                         cATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAAc                             <  1:1043111/93‑1 (MQ=255)

                                                          aTGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACt                            <  2:1410519/93‑1 (MQ=255)

                                                           tGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTc                           <  1:163912/93‑1 (MQ=255)

                                                           tGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTc                           <  2:3162413/93‑1 (MQ=255)

                                                           tGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTc                           <  1:3036578/93‑1 (MQ=255)

                                                           tGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTc                           <  1:4113481/93‑1 (MQ=255)

                                                            ggTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCg                          <  1:1225274/93‑1 (MQ=255)

                                                              tCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGtt                        <  1:4980138/93‑1 (MQ=255)

                                                              tCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGtt                        <  2:6294079/93‑1 (MQ=255)

                                                                  aaTATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCAcc                    <  1:2886375/93‑1 (MQ=255)

                                                                   atatGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCAccc                   <  2:1505665/93‑1 (MQ=255)

                                                                      tGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAAt                <  1:1664486/93‑1 (MQ=255)

                                                                       ggCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAAtt               >  1:1021360/1‑93 (MQ=255)

                                                                       ggCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAAtt               >  1:3686882/1‑93 (MQ=255)

                                                                       ggCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAAtt               <  2:4008285/93‑1 (MQ=255)

                                                                        gCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAAttt              <  2:2296746/93‑1 (MQ=255)

                                                                        gCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAAttt              <  1:4712203/93‑1 (MQ=255)

                                                                         cGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CAGCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAATTTg             <  1:1913359/93‑1 (MQ=255)

                                                                         cGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAATTTg             >  1:4257448/1‑93 (MQ=255)

                                                                          gggTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAATTTGt            >  1:4605601/1‑93 (MQ=255)

                                                                           ggTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAATTTGTa           <  1:3744201/93‑1 (MQ=255)

                                                                           ggTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAATTTGTa           <  1:2645059/93‑1 (MQ=255)

                                                                            gTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAATTTGTAc          <  1:396823/93‑1 (MQ=255)

                                                                            gTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAATTTGTAc          >  1:1088997/1‑93 (MQ=255)

                                                                            gTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAATTTGTAc          >  1:1286109/1‑93 (MQ=255)

                                                                             tccTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAATTTGTAcc         <  1:3431429/91‑1 (MQ=255)

                                                                             tGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAATTTGTAcc         <  2:1737935/93‑1 (MQ=255)

                                                                                tCGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAATTTGTACCggg      >  2:724041/1‑93 (MQ=255)

                                                                                 cGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAATTTGTACCgggc     >  1:3661659/1‑92 (MQ=255)

                                                                                 cGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAATTTGTACCGGGt     <  1:2453620/93‑1 (MQ=255)

                                                                                 cGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAATTTGTACCGGGt     >  1:4387141/1‑93 (MQ=255)

                                                                                 cGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAATTTGTACCGGGt     <  2:6154759/93‑1 (MQ=255)

                                                                                 cGT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAATTTGTACCGGGt     >  2:1376316/1‑93 (MQ=255)

                                                                                  gT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAATTTGTACCGGGTc    >  2:312867/1‑93 (MQ=255)

                                                                                  gT‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAATTTGTACCGGGTc    >  2:432093/1‑93 (MQ=255)

                                                                                   t‑‑‑GC‑‑GCTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAATTTGTACCGGGTCa   <  2:5981620/93‑1 (MQ=255)

                                                                                       gc‑‑gcTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGACCTCGTTCACCCAATTTGTACCGGGTCAc  <  2:4848031/93‑1 (MQ=255)

                                                                                       gc‑‑gcTGT‑G‑‑GCGCGC‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAATTTGTACCGGGTCAc  <  1:4269551/93‑1 (MQ=255)

GAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGT‑‑‑GC‑‑GCTGT‑G‑‑G‑GC‑C‑‑CACCGGAATGACCGACGCCG‑ATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAATTTGTACCGGGTCAC  >  NC_000913/3951074‑3951248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`