BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,980,297	(C)_8→9	noncoding (79/87 nt)	leuT →	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,980,289	1	.	C	96.0%	476.5 / 11.8	149	noncoding (71/87 nt)	leuT	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base . (1/5); new base C (45/98); total (46/103)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.36e-01

Only 100 of 190 total aligned reads displayed.

CATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAAC  >  NC_000913/3980197‑3980376

 attattAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑c                                                                                          >  1:1991391/1‑93 (MQ=255)

  ttattaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑cc                                                                                         <  2:2343851/93‑1 (MQ=255)

   tattaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑ccc                                                                                        <  1:4721836/93‑1 (MQ=255)

   tattaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑ccc                                                                                        <  1:2709921/93‑1 (MQ=255)

      taGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAAG‑T‑‑ccc‑ccc                                                                                    >  1:3671440/1‑93 (MQ=255)

        gAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑ccc‑ccccc                                                                                  >  2:2044030/1‑93 (MQ=255)

        gAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAAG‑T‑‑ccc‑ccccc                                                                                  >  2:3496330/1‑93 (MQ=255)

         aaGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑ccc‑cccccc                                                                                 <  1:4587606/93‑2 (MQ=255)

         aaGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑G‑‑ccc‑cccccc                                                                                 <  2:1792406/93‑2 (MQ=255)

          aGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑ccc‑cccccct                                                                                <  2:3596532/93‑3 (MQ=255)

          aGTTGTGACAATGCGAAGGTGGCGAAATTGGTAGCCGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑ccc‑cccccct                                                                                <  2:768275/93‑3 (MQ=255)

           gTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑ccc‑cccccctc                                                                               >  2:6040593/1‑90 (MQ=255)

           gTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑ccc‑cccccctc                                                                               <  2:369150/93‑4 (MQ=255)

           gTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑ccc‑cccccctc                                                                               >  2:4948481/1‑90 (MQ=255)

                gACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGcacc                                                                           <  1:103559/93‑1 (MQ=255)

                 aCAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGcacca                                                                          <  2:4023954/93‑1 (MQ=16)

                  cAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGcaccac                                                                         <  1:4835918/93‑1 (MQ=16)

                    aTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGa                                                                       <  2:3280606/93‑1 (MQ=16)

                     tGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑c                                                                     <  2:2717349/93‑1 (MQ=255)

                     tGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑c                                                                     <  2:127722/93‑1 (MQ=255)

                     tGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑c                                                                     <  1:1455288/93‑1 (MQ=255)

                      gCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGCCGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑Ct                                                                    <  2:5295196/93‑1 (MQ=255)

                      gCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑Ct                                                                    <  1:4895585/93‑1 (MQ=255)

                      gCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑Ct                                                                    >  2:5648186/1‑93 (MQ=255)

                      gCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑Ct                                                                    <  2:541183/93‑1 (MQ=255)

                      gCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑Ct                                                                    <  1:3103018/93‑1 (MQ=255)

                      gCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑Ct                                                                    <  1:2835946/93‑1 (MQ=255)

                      gCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑Ct                                                                    >  2:5110536/1‑93 (MQ=255)

                        gAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑Cttt                                                                  >  1:305370/1‑93 (MQ=255)

                        gAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑Cttt                                                                  <  1:292160/93‑1 (MQ=255)

                         aaGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTa                                                                 >  2:6304388/1‑93 (MQ=255)

                         aaGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTa                                                                 <  1:3881153/93‑1 (MQ=255)

                         aaGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTa                                                                 >  1:5742205/1‑93 (MQ=255)

                          aGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTaa                                                                >  1:2771589/1‑93 (MQ=255)

                          aGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCCCACCACGA‑CTTTaa                                                                >  1:1034729/1‑93 (MQ=255)

                          aGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAAGTT‑‑CCC‑CCCCCTCTCACCACCA‑CCTTaa                                                                >  1:387983/1‑93 (MQ=11)

                            gTGGCGGAATTGGTAGACGCGCTAGCTTCATGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAg                                                              <  1:2497738/93‑1 (MQ=255)

                             tGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGa                                                             <  2:3825348/93‑1 (MQ=255)

                              ggCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAAG‑T‑‑CCC‑CCCCCCCGCACCACCACCTTTaaaaaa                                                            >  1:3392239/1‑93 (MQ=11)

                               gCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAAt                                                           <  1:3663035/93‑1 (MQ=255)

                               gCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAAt                                                           <  1:503035/93‑1 (MQ=255)

                                cGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAAtt                                                          >  2:2692846/1‑93 (MQ=255)

                                  gAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGa                                                        >  2:6253541/1‑93 (MQ=255)

                                  gAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGa                                                        >  1:1075538/1‑93 (MQ=255)

                                    aTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAAc                                                      <  2:5033965/93‑1 (MQ=255)

                                    aTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAAc                                                      >  1:865947/1‑93 (MQ=255)

                                      tGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTa                                                    >  2:5739697/1‑93 (MQ=255)

                                      tGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTa                                                    <  1:2082713/93‑1 (MQ=255)

                                       ggTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTCAACAATTGAACtca                                                   >  1:5204959/1‑91 (MQ=17)

                                        gTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTaaa                                                  <  2:5882648/93‑1 (MQ=255)

                                         tAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTTAAG‑TC‑CCC‑CCCCCTCGCCCCACGA‑CCTTTAAGAATTGAGCTaaaa                                                 >  1:3568776/1‑93 (MQ=17)

                                           gACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑CCCGCCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTACAAAt                                               >  2:3017755/1‑93 (MQ=255)

                                             cgcgCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTc                                             <  2:2941601/93‑1 (MQ=255)

                                             cgcgCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTc                                             <  1:4362292/93‑1 (MQ=255)

                                              gcgcTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCa                                            <  2:5141182/93‑1 (MQ=255)

                                                ccTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCaaa                                          <  1:3236910/92‑1 (MQ=255)

                                                 cTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCaaaa                                         >  2:6156614/1‑93 (MQ=255)

                                                   aGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCCCCCCCACCTTTAAAAAATT‑AAATAAAAAATCaaaaaa                                       >  1:3693390/1‑92 (MQ=9)

                                                   aGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAg                                       <  2:2953444/93‑1 (MQ=255)

                                                   aGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAg                                       >  2:3912215/1‑93 (MQ=255)

                                                    gCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGc                                      <  1:2629419/93‑1 (MQ=255)

                                                     cTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCa                                     <  1:2019963/93‑1 (MQ=255)

                                                     cTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCa                                     <  2:3988646/93‑1 (MQ=255)

                                                     cTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAg                                    <  2:1084166/93‑1 (MQ=255)

                                                      ttCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAg                                    <  2:1250894/93‑1 (MQ=255)

                                                      ttCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAg                                    <  1:73192/93‑1 (MQ=255)

                                                      tacAGATGTTAGTGGCCTTACGGGCTTGGCGGTTCAAGCC‑‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAg                                    <  1:5576633/91‑1 (MQ=25)

                                                       tCAGGTGTTAGTGTCCTTACGGGCGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAAGTGAACTAAAAATTCAAAAAGCAGt                                   <  1:127557/93‑1 (MQ=25)

                                                        cAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGCC‑‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTa                                  <  1:6238664/93‑1 (MQ=255)

                                                         aGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAACAGTAt                                 >  2:5809280/1‑93 (MQ=255)

                                                          ggTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAtt                                <  2:4149701/93‑1 (MQ=255)

                                                           gtgtTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAttt                               <  1:2054885/93‑1 (MQ=255)

                                                           gtgtTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAttt                               <  1:3305974/93‑1 (MQ=255)

                                                            tgtTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTc                              <  1:495912/93‑1 (MQ=255)

                                                            tgtTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTc                              <  2:4233212/93‑1 (MQ=255)

                                                            tgtTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTc                              >  1:687528/1‑93 (MQ=255)

                                                              ttAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCCCCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCgg                            <  2:883120/93‑1 (MQ=255)

                                                               tAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGc                           <  1:1761526/93‑1 (MQ=255)

                                                               tAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGc                           <  2:1464933/93‑1 (MQ=255)

                                                                   gtCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGt                       <  2:3699461/93‑1 (MQ=255)

                                                                   gtCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGt                       <  2:4481121/93‑1 (MQ=255)

                                                                    tCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTa                      <  1:2285971/93‑1 (MQ=255)

                                                                    tCCTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAAATAAAAATTCAAAAAGCAGTATTTCGGCGAGTa                      >  2:2681252/1‑93 (MQ=255)

                                                                      cTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgc                    <  2:222621/93‑1 (MQ=255)

                                                                      cTTACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgc                    <  1:3749114/93‑1 (MQ=255)

                                                                       ttACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgcg                   <  2:3845142/93‑1 (MQ=255)

                                                                        tccGGACGTGGGGTTTCAAGCC‑‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgcgc                  <  1:1117065/91‑1 (MQ=38)

                                                                        tACGGACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgcgc                  <  1:5389982/93‑1 (MQ=255)

                                                                            gACGTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCt              >  2:3637314/1‑93 (MQ=255)

                                                                             aCGTGGGGATTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCtt             <  1:4276425/93‑1 (MQ=255)

                                                                               gTGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTgg           <  1:1488979/93‑1 (MQ=255)

                                                                                tGGGGGTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGt          <  2:4752649/93‑1 (MQ=255)

                                                                                tGGGGGTTCAAG‑T‑‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTa         >  1:4037319/1‑93 (MQ=255)

                                                                                 gggggTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTa         >  2:3054350/1‑93 (MQ=255)

                                                                                 gggggTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTa         <  2:3092180/93‑1 (MQ=255)

                                                                                  ggggTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAg        >  2:3707930/1‑93 (MQ=255)

                                                                                  ggggTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGAGCAGCTTCGTAg        >  1:2377960/1‑93 (MQ=255)

                                                                                  ggggTTCAAG‑CCCCCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTa         <  1:3087345/93‑1 (MQ=255)

                                                                                   gggTTCAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAgc       >  2:2519025/1‑93 (MQ=255)

                                                                                        cAAG‑TC‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAAc  <  1:3591012/93‑1 (MQ=255)

CATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑CCC‑CCCCCTCGCACCACGA‑CTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAAC  >  NC_000913/3980197‑3980376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`