Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 659,755 G→A P283S (CCG→TCG)  ybeF ← putative transcriptional regulator LYSR‑type

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913659,7550GA99.6% 910.5 / NA 235P283S (CCG→TCG) ybeFputative transcriptional regulator LYSR‑type
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (124/110);  total (125/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 242 total aligned reads displayed.

AACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACTGTTGGCAACCA  >  NC_000913/659666‑659844
                                                                                         |                                                                                         
 aCATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAaaaa                                                                                       >  1:3420145/1‑93 (MQ=255)
  cATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAg                                                                                      >  1:2706197/1‑93 (MQ=255)
   aTCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAgg                                                                                     <  1:3638350/93‑1 (MQ=255)
    tCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGc                                                                                    <  1:2553178/93‑1 (MQ=255)
     cTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCa                                                                                   <  1:2005691/93‑1 (MQ=255)
       aTTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGATCCTCATTTAAGGACGAAACAGGCAGt                                                                                 >  2:2839643/1‑93 (MQ=255)
        ttACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGtt                                                                                <  2:3012508/93‑1 (MQ=255)
         tACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGttt                                                                               <  2:6325866/93‑1 (MQ=255)
         tACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGttt                                                                               <  1:6383601/93‑1 (MQ=255)
          aCTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGtttt                                                                              >  2:539614/1‑93 (MQ=255)
           cTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGttttt                                                                             >  1:4569884/1‑93 (MQ=255)
            tCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTc                                                                            <  1:657359/93‑1 (MQ=255)
            tCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTc                                                                            <  1:130505/93‑1 (MQ=255)
             ccGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTcc                                                                           <  2:1929356/93‑1 (MQ=255)
             ccGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTcc                                                                           <  1:4149167/93‑1 (MQ=255)
             ccGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTcc                                                                           <  2:4306094/93‑1 (MQ=255)
                tGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGc                                                                        <  2:5042180/93‑1 (MQ=255)
                  cAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTCAAGGACGAAAAAGGCAGTTTTTCCAGCgg                                                                      <  1:4790552/93‑1 (MQ=255)
                    ggATGGGATCACGCAGTCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGcc                                                                    >  2:200197/1‑93 (MQ=255)
                    ggATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGcc                                                                    <  2:5205110/93‑1 (MQ=255)
                    ggATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGcc                                                                    >  2:5615206/1‑93 (MQ=255)
                       tGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCcagc                                                                 <  1:1214079/93‑1 (MQ=255)
                        gggATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCcagca                                                                <  2:6029560/93‑1 (MQ=255)
                        gggATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCcagca                                                                <  2:4828773/93‑1 (MQ=255)
                        gggATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCcagca                                                                <  1:913207/93‑1 (MQ=255)
                         ggATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCcagcag                                                               >  1:491705/1‑93 (MQ=255)
                          gATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCagcagc                                                              <  1:1194430/93‑1 (MQ=255)
                            tCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCgg                                                            >  1:3673384/1‑93 (MQ=255)
                            tCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCgg                                                            <  1:4251297/93‑1 (MQ=255)
                              aCGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCt                                                          >  2:5935355/1‑93 (MQ=255)
                                  aGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAAc                                                      >  1:61507/1‑93 (MQ=255)
                                  aGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAAc                                                      >  2:731622/1‑93 (MQ=255)
                                  aGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAAc                                                      >  1:783787/1‑93 (MQ=255)
                                  aGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAAc                                                      >  1:1372517/1‑93 (MQ=255)
                                   ggCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACa                                                     >  2:6410118/1‑93 (MQ=255)
                                    gCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAg                                                    >  1:3997357/1‑93 (MQ=255)
                                     cTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAgg                                                   >  1:3732471/1‑93 (MQ=255)
                                     cTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAgg                                                   <  2:4033068/93‑1 (MQ=255)
                                      tGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGt                                                  <  2:2862774/93‑1 (MQ=255)
                                      tGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGt                                                  <  2:3953656/93‑1 (MQ=255)
                                       gAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGtt                                                 >  1:5318362/1‑93 (MQ=255)
                                        aaTTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTta                                                <  2:1165827/93‑1 (MQ=255)
                                        aaTTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTta                                                >  2:4165885/1‑93 (MQ=255)
                                        aaTTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTta                                                >  2:6163059/1‑93 (MQ=255)
                                         aTTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTtat                                               >  1:5242555/1‑93 (MQ=255)
                                         aTTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTtat                                               <  1:5757696/93‑1 (MQ=255)
                                         aTTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTtat                                               <  2:2126921/93‑1 (MQ=255)
                                          tttGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTtata                                              >  2:1982637/1‑93 (MQ=255)
                                             gttgtAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATaaaa                                           >  2:4369642/1‑93 (MQ=255)
                                               tgtAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAAc                                         <  1:2065440/93‑1 (MQ=255)
                                                gtAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACg                                        <  1:6061142/93‑1 (MQ=255)
                                                  aGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGc                                      <  2:3912430/93‑1 (MQ=255)
                                                  aGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGc                                      <  2:3560253/93‑1 (MQ=255)
                                                  aGTGAATAGAGAAATCTATTTGCTCCTCATGTAAGGACGAAAAAGGCAGTTTTTCCGGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGc                                      <  2:5986738/93‑1 (MQ=255)
                                                     ggATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAGAGAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTgg                                   <  2:3495343/93‑1 (MQ=255)
                                                     ggATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTgg                                   <  1:2426279/93‑1 (MQ=255)
                                                      gATAGAGAAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTggg                                  <  1:4626593/93‑1 (MQ=255)
                                                          gagaAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGaata                              >  1:1761809/1‑93 (MQ=255)
                                                           agaAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGCCTGGGaacaa                             >  1:4177900/1‑93 (MQ=255)
                                                            gaAATCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGaataat                            >  2:3059938/1‑93 (MQ=255)
                                                             aaaTCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATc                           <  2:4642923/93‑1 (MQ=255)
                                                             aaaTCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATc                           <  2:5182197/93‑1 (MQ=255)
                                                              aaTCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCg                          >  1:5646624/1‑93 (MQ=255)
                                                               aTCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGc                         >  2:2565647/1‑93 (MQ=255)
                                                               aTCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGc                         <  2:5455670/93‑1 (MQ=255)
                                                                tCTATTTGCTCCTCATTTAAGGACGAAAAAGGCCGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAAATATCGCt                        >  1:3136045/1‑93 (MQ=255)
                                                                tCTATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCt                        <  2:4898691/93‑1 (MQ=255)
                                                                  tATTTGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTaa                      >  2:3434721/1‑93 (MQ=255)
                                                                    tttGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACa                    <  2:3150439/93‑1 (MQ=255)
                                                                     ttGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACAt                   >  2:2244436/1‑93 (MQ=255)
                                                                     ttGCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACAt                   <  1:504050/93‑1 (MQ=255)
                                                                       gCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGt                 <  2:1048260/93‑1 (MQ=255)
                                                                       gCTCCTCATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGt                 <  2:5872446/93‑1 (MQ=255)
                                                                        ctcctcATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTc                >  1:628971/1‑93 (MQ=255)
                                                                         tcctcATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCa               >  2:1446805/1‑93 (MQ=255)
                                                                         tcctcATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCa               >  2:4882607/1‑93 (MQ=255)
                                                                           ctcATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACt             >  2:3331565/1‑93 (MQ=255)
                                                                           ctcATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACt             >  1:1702007/1‑93 (MQ=255)
                                                                            tcATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACTg            >  2:2402729/1‑93 (MQ=255)
                                                                            tcATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACTg            >  2:3802762/1‑93 (MQ=255)
                                                                            tcATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACTg            >  1:670297/1‑93 (MQ=255)
                                                                            tcATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACTg            <  1:4376508/93‑1 (MQ=255)
                                                                            tcATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAAAATGTCACTg            >  1:114512/1‑93 (MQ=255)
                                                                             cgtttAAGGACGAAAAAGGCAGTTTATCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACTGt           <  2:1536052/91‑1 (MQ=255)
                                                                             cATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACTGt           >  2:1379828/1‑93 (MQ=255)
                                                                             cATTTAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACTGt           <  2:5668145/93‑1 (MQ=255)
                                                                               tttAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACTGTTg         <  1:334835/93‑1 (MQ=255)
                                                                               tttAAGGACGAAAAAGGCAGTTTTTCCAGCGACCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACTGTTg         <  1:963288/93‑1 (MQ=255)
                                                                                 tAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACTGTTGGc       <  2:1651/93‑1 (MQ=255)
                                                                                 tAAGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGGTATAAAAACGGCTGGGAATAATTGCCAACATGTCACTGGTGGc       >  1:238380/1‑93 (MQ=255)
                                                                                  aaGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGTCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACTGTTggaa      >  2:5324898/1‑91 (MQ=255)
                                                                                  aaGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACTGTTGGCa      <  2:1686062/93‑1 (MQ=255)
                                                                                  aaGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACTGTTGGCa      >  1:3016711/1‑93 (MQ=255)
                                                                                  aaGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACTGTTGGCa      <  1:4129468/93‑1 (MQ=255)
                                                                                  aaGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACTGTTGGCa      >  2:210853/1‑93 (MQ=255)
                                                                                   aGGACGGAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGGATAATCGCTAACATGTCACTGTTGGCaa     >  2:5573804/1‑93 (MQ=255)
                                                                                   aGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACTGTTGGCaa     >  1:5547024/1‑93 (MQ=255)
                                                                                   aGGACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACTGTTGGCaa     >  1:1092560/1‑93 (MQ=255)
                                                                                    ggACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACTGTTGGCAAc    <  1:1655269/93‑1 (MQ=255)
                                                                                     gACGAAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACTGTTGGCAAcc   <  1:1247277/93‑1 (MQ=255)
                                                                                         |                                                                                         
AACATCTATTACTCCGTGCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACTGTTGGCAACCA  >  NC_000913/659666‑659844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑