BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	697,801	A→G	D200D (GAT→GAC)	asnB ←	asparagine synthetase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	697,801	0	A	G	99.2%	796.7 / ‑2.5	235	D200D (GAT→GAC)	asnB	asparagine synthetase B
Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (119/114); total (121/114)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.12e-01

Only 100 of 246 total aligned reads displayed.

GTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGT  >  NC_000913/697711‑697890

 tAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCg                                                                                        <  2:4795261/93‑1 (MQ=255)

  aaGGCACATCAGACATCAGATGGCTTTTAACTGAATCATCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGcgtcgt                                                                                       >  2:6398797/1‑93 (MQ=255)

   aGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTa                                                                                      >  1:3210048/1‑93 (MQ=255)

    ggCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAg                                                                                     <  1:2981460/93‑1 (MQ=255)

      cacaTCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTc                                                                                   <  1:3386142/93‑1 (MQ=255)

      cacaTCAGACATCAGATGGCTTTTAACTGAACCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTc                                                                                   <  1:4848313/93‑1 (MQ=255)

       acatcagacatcagACGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCg                                                                                  >  1:1440243/1‑93 (MQ=255)

        catcagacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCCCGTTATCTTTCACCGCGTCGTAGTCGa                                                                                 <  1:1176398/93‑1 (MQ=255)

         atcagacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGaa                                                                                >  1:3606686/1‑93 (MQ=255)

          tcagacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAAc                                                                               <  1:3197748/93‑1 (MQ=255)

           cagacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAAcc                                                                              >  2:569770/1‑93 (MQ=255)

           cagacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAAcc                                                                              <  2:2855595/93‑1 (MQ=255)

            agacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCa                                                                             >  2:3630135/1‑93 (MQ=255)

             gacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAg                                                                            >  1:2224073/1‑93 (MQ=255)

              acatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGt                                                                           <  2:2280692/93‑1 (MQ=255)

               aaacagaTGGCTTTTAACTGAAACTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTc                                                                          <  1:5394609/90‑1 (MQ=255)

                 tcagaTGGCTTTTAACTGAATCTTCCAGAGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACAGCGTCGTAGTCGAACCAGTagc                                                                        >  2:3855213/1‑93 (MQ=255)

                  cagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTcgcg                                                                       >  2:5435000/1‑93 (MQ=255)

                   agaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCga                                                                      >  1:3752303/1‑93 (MQ=255)

                    gaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCgat                                                                     >  1:795357/1‑93 (MQ=255)

                    gaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCgat                                                                     <  1:4325035/93‑1 (MQ=255)

                    gaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCgat                                                                     <  2:5139161/93‑1 (MQ=255)

                    gaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCgat                                                                     <  1:5579126/93‑1 (MQ=255)

                    gaTGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCgat                                                                     >  1:1553789/1‑93 (MQ=255)

                      tGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCgatga                                                                   <  1:2437602/93‑1 (MQ=255)

                       ggCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCgatgat                                                                  <  2:5483708/93‑1 (MQ=255)

                        gCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATa                                                                 >  2:729536/1‑93 (MQ=255)

                         cTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAg                                                                >  1:2508671/1‑93 (MQ=255)

                          ttttAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGt                                                               >  1:4955303/1‑93 (MQ=255)

                           tttAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTa                                                              >  1:3042795/1‑93 (MQ=255)

                            ttAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTaa                                                             >  2:1000910/1‑93 (MQ=255)

                              aaCTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTTACCGCGTCGTAGTCGGACCAGTCGCGATGATAGTAAGa                                                           >  1:3553427/1‑93 (MQ=255)

                                cTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAAc                                                         <  1:755032/93‑1 (MQ=255)

                                 tGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACg                                                        <  2:634626/93‑1 (MQ=255)

                                 tGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACg                                                        >  1:5221370/1‑93 (MQ=255)

                                  gAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACgg                                                       >  1:2099866/1‑93 (MQ=255)

                                    aTCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGAt                                                     <  1:4482175/93‑1 (MQ=255)

                                    aTCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGAt                                                     >  1:1923853/1‑93 (MQ=255)

                                      cTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGGTGATAGTAAGAACGGAttt                                                   <  1:6467425/93‑1 (MQ=255)

                                       ttCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTc                                                  <  2:831552/93‑1 (MQ=255)

                                       ttCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTc                                                  <  2:2981216/93‑1 (MQ=255)

                                        tCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAAAGGATTTCg                                                 >  2:2458707/1‑93 (MQ=255)

                                        tCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCg                                                 >  1:3688710/1‑93 (MQ=255)

                                         ccAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGc                                                >  2:1596871/1‑93 (MQ=255)

                                            gTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTTGCGATGATAGTAAGAACGGATTTCGCCGt                                             >  2:5477494/1‑93 (MQ=255)

                                            gTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGt                                             >  2:4727863/1‑93 (MQ=255)

                                            gTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCAACGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGt                                             >  2:4532818/1‑93 (MQ=255)

                                              gCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTcc                                           <  1:5697040/93‑1 (MQ=255)

                                              gCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTcc                                           <  1:4574414/93‑1 (MQ=255)

                                              gCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTcc                                           <  2:5546707/93‑1 (MQ=255)

                                               ccTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCt                                          <  2:1064898/93‑1 (MQ=255)

                                                 tGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTgg                                        >  1:455573/1‑93 (MQ=255)

                                                 tGACGCAGCGCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTgg                                        <  2:3931361/93‑1 (MQ=255)

                                                  gACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCtcgg                                       >  1:1354364/1‑90 (MQ=255)

                                                  gACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGc                                       <  2:5979428/93‑1 (MQ=255)

                                                   aCGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCt                                      >  1:1881956/1‑93 (MQ=255)

                                                   aCGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCt                                      >  2:6078498/1‑93 (MQ=255)

                                                    cGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTc                                     >  1:1738786/1‑93 (MQ=255)

                                                     gCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTcc                                    <  1:6101005/93‑1 (MQ=255)

                                                     gCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTcc                                    <  1:478122/93‑1 (MQ=255)

                                                       aGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCcac                                  <  2:317141/93‑1 (MQ=255)

                                                       aGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCcac                                  <  2:2103606/93‑1 (MQ=255)

                                                       aGCTCGTTTTTGGCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCcac                                  <  2:4907367/93‑1 (MQ=255)

                                                        gCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCcaca                                 >  2:1371476/1‑93 (MQ=255)

                                                        gCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCcaca                                 >  2:4061089/1‑93 (MQ=255)

                                                         cTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACaa                                >  2:2764581/1‑93 (MQ=255)

                                                           cGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAAt                              <  2:3046750/93‑1 (MQ=255)

                                                           cGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAAt                              <  1:2761251/93‑1 (MQ=255)

                                                           cGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAAt                              <  1:5633473/93‑1 (MQ=255)

                                                           cGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAAt                              >  1:2239433/1‑93 (MQ=255)

                                                            gTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATa                             <  2:2941850/93‑1 (MQ=255)

                                                            gTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATa                             <  2:2067520/93‑1 (MQ=255)

                                                            gTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATa                             >  2:1696975/1‑93 (MQ=255)

                                                            gTTTTTGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATa                             <  2:3483231/93‑1 (MQ=255)

                                                             tttttGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAg                            <  1:1992043/93‑1 (MQ=255)

                                                               tttGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCt                          <  2:1244676/93‑1 (MQ=255)

                                                               tttGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCt                          <  1:408310/93‑1 (MQ=255)

                                                                ttGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTc                         <  2:5615011/93‑1 (MQ=255)

                                                                 tGTCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTcc                        <  2:4217216/93‑1 (MQ=255)

                                                                   tCGGTCACGTTATCTTTCCCCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTcccc                      <  1:5365995/93‑1 (MQ=255)

                                                                   tCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTcccc                      <  2:2491513/93‑1 (MQ=255)

                                                                   tCGGTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTcccc                      >  2:5279951/1‑93 (MQ=255)

                                                                      gTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCCCAAATAGCTCCccgcc                   >  1:12828/1‑93 (MQ=255)

                                                                      gTCACGTTATCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCccgcc                   >  1:5141368/1‑93 (MQ=255)

                                                                         aCGTTATCTTTCACCGCGTCGTAGTCGAAGCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCggg                >  1:5804304/1‑93 (MQ=255)

                                                                              aTCTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAActc           <  1:2146113/93‑1 (MQ=255)

                                                                                cTTTCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCtt         <  1:1500385/93‑1 (MQ=255)

                                                                                cTTTCACCGCGTAGTAGTCGAACCAGTCGAGATGATAGTAAGACCGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCtt         <  2:6116254/93‑1 (MQ=255)

                                                                                 tttCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCttt        <  1:122075/93‑1 (MQ=255)

                                                                                 tttCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCttt        <  2:5238181/93‑1 (MQ=255)

                                                                                   tCACCGCGTCGTAGTCGAACCAGTCGCGATGTTAGTAAGAACGGATTTCGCCGTCCTGGGTCCACAAATAGCTCCCCGCCGGGAACTCTTTaa      >  1:2626232/1‑93 (MQ=255)

                                                                                   tCACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTaa      >  2:1824260/1‑93 (MQ=255)

                                                                                    cACCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAAt     >  1:3834590/1‑93 (MQ=255)

                                                                                     cccGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATc    <  1:4051422/92‑1 (MQ=255)

                                                                                     cccGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATc    <  1:3476877/92‑1 (MQ=255)

                                                                                     aCCGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATc    <  1:6133092/93‑1 (MQ=255)

                                                                                      ccGCGTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCCCCGGGCACTCTTTAATCg   >  1:1515484/1‑93 (MQ=255)

                                                                                       cgcgTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGt  <  2:3088947/93‑1 (MQ=255)

                                                                                       cgcgTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGt  >  2:5898216/1‑93 (MQ=255)

                                                                                       cgcgTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGt  <  1:623496/93‑1 (MQ=255)

GTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGT  >  NC_000913/697711‑697890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`