BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	704,130	A→G	I322V (ATC→GTC)	nagE →	PTS system, N‑acetylglucosamine‑specific enzyme IIABC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	704,130	0	A	G	100.0%	611.7 / NA	179	I322V (ATC→GTC)	nagE	PTS system, N‑acetylglucosamine‑specific enzyme IIABC
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (79/100); total (79/100)

Only 100 of 185 total aligned reads displayed.

TACCTCCTGCACGCACTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTATCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTAC  >  NC_000913/704040‑704219

tACCTCCTGCACGCACTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTc                                                                                         <  1:1156517/93‑1 (MQ=255)

tACCTCCTGCACGCACTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTc                                                                                         <  1:5402474/93‑1 (MQ=255)

  gtgcctGCACGCACGGCTGATCGGAATCAGCCGGTTTGTGGCAACGCTGCTGGGTATCCAGTCGGGCTTCTCTTTCTCTTCGGGGGCTGTCGa                                                                                       <  1:4893914/90‑1 (MQ=255)

   ctcctGCACGCACTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGAc                                                                                      <  2:1425030/93‑1 (MQ=255)

    tcctGCACGCACTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACt                                                                                     >  1:6068588/1‑93 (MQ=255)

    tcctGCACGCACTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACt                                                                                     <  2:5638872/93‑1 (MQ=255)

    tcctGCACGCACTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACt                                                                                     >  1:988060/1‑93 (MQ=255)

          acgcacTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGt                                                                               <  1:4434012/93‑1 (MQ=255)

          acgcacTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGt                                                                               <  2:2803217/93‑1 (MQ=255)

          acgcacTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGt                                                                               <  1:3871681/93‑1 (MQ=255)

           cgcacTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGtt                                                                              <  1:3871907/93‑1 (MQ=255)

             cacTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGa                                                                            >  1:5362198/1‑93 (MQ=255)

              acTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGAt                                                                           <  2:2574162/93‑1 (MQ=255)

                 gctgACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGta                                                                        <  2:2000325/93‑1 (MQ=255)

                 gctgACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGta                                                                        <  2:2867796/93‑1 (MQ=255)

                   tgACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGtata                                                                      <  1:3386963/93‑1 (MQ=255)

                    gACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATaa                                                                     >  1:6176245/1‑93 (MQ=255)

                    gACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATaa                                                                     >  1:5257199/1‑93 (MQ=255)

                    gACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATaa                                                                     >  2:4755177/1‑93 (MQ=255)

                      ccGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAAcc                                                                   <  1:6220677/93‑1 (MQ=255)

                      ccGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAAcc                                                                   <  2:2237639/93‑1 (MQ=255)

                      ccGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAAcc                                                                   >  2:3614331/1‑93 (MQ=255)

                      ccGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAAcc                                                                   <  2:3839596/93‑1 (MQ=255)

                       cGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCt                                                                  <  1:5577844/93‑1 (MQ=255)

                        ggTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTg                                                                 >  2:5893094/1‑93 (MQ=255)

                         gTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTgc                                                                <  1:4716780/93‑1 (MQ=255)

                         gTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTgc                                                                >  1:2444063/1‑93 (MQ=255)

                         gTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTgc                                                                >  1:5160519/1‑93 (MQ=255)

                          tATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTgcc                                                               >  2:6202066/1‑93 (MQ=255)

                           aTCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTgccg                                                              <  1:2698922/93‑1 (MQ=255)

                            tCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTgccgg                                                             >  1:5997085/1‑93 (MQ=255)

                            tCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTgccgg                                                             >  1:3253049/1‑93 (MQ=255)

                             cAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTgccggc                                                            <  1:3527051/93‑1 (MQ=255)

                             cAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTgccggc                                                            <  2:1885772/93‑1 (MQ=255)

                              aGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTgccggcc                                                           <  1:5757589/93‑1 (MQ=255)

                              aGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTgccggcc                                                           <  1:2773596/93‑1 (MQ=255)

                              aGCCTGTTTGTGGAAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTgccggcc                                                           >  1:2920758/1‑93 (MQ=255)

                               tccTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTgccggccg                                                          <  1:1989371/92‑1 (MQ=255)

                                 cTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGgccgcc                                                        >  1:2866758/1‑93 (MQ=255)

                                 cTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGgccgcc                                                        >  1:183011/1‑93 (MQ=255)

                                 cTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGgccgcc                                                        >  1:2113444/1‑93 (MQ=255)

                                  tGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCgcca                                                       <  2:5930870/93‑1 (MQ=255)

                                  tGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCgcca                                                       <  2:1317883/93‑1 (MQ=255)

                                  tGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCgcca                                                       <  1:5733353/93‑1 (MQ=255)

                                         ggCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGaa                                                >  1:4586819/1‑93 (MQ=255)

                                             aCGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGGCGCCAGCCAGAACGTc                                            >  1:2601849/1‑93 (MQ=255)

                                             aCGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTc                                            <  2:1123584/93‑1 (MQ=255)

                                              cGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCt                                           <  2:236825/93‑1 (MQ=255)

                                              cGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCt                                           >  1:4023588/1‑93 (MQ=255)

                                              cGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGCATAACCTGCCGGCCGCCAGCCAGAAccact                                           >  2:6369675/1‑89 (MQ=255)

                                               cctgctgGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTg                                          <  1:1719612/92‑1 (MQ=255)

                                                ctgctgGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTgg                                         >  2:1191120/1‑93 (MQ=255)

                                                   ctgGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGAtg                                      <  1:5496106/93‑1 (MQ=255)

                                                    tgGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGAtgc                                     >  2:3453105/1‑93 (MQ=255)

                                                    tgGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGAtgc                                     <  1:5278516/93‑1 (MQ=255)

                                                     tggTATCCACCCCGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGAtgct                                    <  1:1921016/92‑1 (MQ=255)

                                                       gTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGAtgctgc                                  >  2:5739958/1‑93 (MQ=255)

                                                       gTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGAtgctgc                                  <  1:1056332/93‑1 (MQ=255)

                                                       gTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGAtgctgc                                  >  1:680193/1‑93 (MQ=255)

                                                        tATCCACGCGGTCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATACCCTGCCGGCCGCCAGCCAGAACGTCTGGATgctgct                                 <  1:941043/93‑1 (MQ=255)

                                                        tATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATgctgct                                 <  1:2316068/93‑1 (MQ=255)

                                                        tATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATgctgct                                 <  1:1419890/93‑1 (MQ=255)

                                                          gctaCGCGGTCTGCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTTCCGGCCGCCAGCCTGAACGTCTGGATGCTGCTgg                               >  1:6475654/4‑93 (MQ=255)

                                                           ccACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGt                              <  1:2512512/93‑1 (MQ=255)

                                                            cACGTGGACTTCTTTTTCTCGGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTg                             <  1:4664592/93‑1 (MQ=255)

                                                            cACGCGGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTg                             >  1:276006/1‑93 (MQ=255)

                                                             ccgcgGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGa                            <  1:4259900/92‑1 (MQ=255)

                                                              cgcgGGCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGAt                           <  1:5902417/93‑1 (MQ=255)

                                                                 gggCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATggg                        >  1:4873786/1‑93 (MQ=255)

                                                                  ggCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGc                       >  2:675673/1‑93 (MQ=255)

                                                                   gCTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCg                      <  1:2135509/93‑1 (MQ=255)

                                                                   gCTTCTCTTTCTCTGCGGGCGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCg                      >  1:6311772/1‑93 (MQ=255)

                                                                    cTTCTCTTTCTCTGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGt                     >  2:4303245/1‑93 (MQ=255)

                                                                     ttctctttctctGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGtt                    >  2:5165409/1‑93 (MQ=255)

                                                                      tctctttctctGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTa                   <  1:4488232/93‑1 (MQ=255)

                                                                       ctctttctctGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTAt                  >  2:1092977/1‑93 (MQ=255)

                                                                        tctttctctGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTAtc                 >  2:533230/1‑93 (MQ=255)

                                                                        tctttctctGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTAtc                 <  2:172760/93‑1 (MQ=255)

                                                                        tctttctctGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTAtc                 >  2:4388968/1‑93 (MQ=255)

                                                                         ctttctctGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTAtct                <  2:6016364/93‑1 (MQ=255)

                                                                           ttctctGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTAtcttc              <  1:6253072/93‑1 (MQ=255)

                                                                           ttctctGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTAtcttc              <  1:3021483/93‑1 (MQ=255)

                                                                            tctctGCGGGGGCTGTCGCCTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGACCGTCTGGATGCTGCTGGTGATGGGCGTTAtcttct             <  1:2480239/93‑1 (MQ=255)

                                                                            tctctGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTAtcttct             <  2:5848686/93‑1 (MQ=255)

                                                                            tctctGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTAtcttct             <  2:1439348/93‑1 (MQ=255)

                                                                             ctctGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATcttctt            >  2:4690383/1‑93 (MQ=255)

                                                                             ctctGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATcttctt            <  2:6027626/93‑1 (MQ=255)

                                                                              tctGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCttcttc           >  2:964138/1‑93 (MQ=255)

                                                                              tctGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCttcttc           <  1:2043000/93‑1 (MQ=255)

                                                                                tGCGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGCGATGGGCGTTATCTTCTTCGc         >  1:4528938/1‑93 (MQ=255)

                                                                                  cGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTa       >  2:1854075/1‑93 (MQ=255)

                                                                                  cGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTa       <  2:3518834/93‑1 (MQ=255)

                                                                                  cGGGGGCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTa       >  2:4427169/1‑93 (MQ=255)

                                                                                   gggggCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTAt      >  1:502724/1‑93 (MQ=255)

                                                                                   gcgggCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTAt      >  2:1946723/3‑93 (MQ=255)

                                                                                    ggggCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATc     <  2:767544/93‑1 (MQ=255)

                                                                                     gggCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCt    <  1:2566862/93‑1 (MQ=255)

                                                                                     gggCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCt    >  2:4201402/1‑93 (MQ=255)

                                                                                      ggCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTa   >  1:3218392/1‑93 (MQ=255)

                                                                                       gCTGTCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTAc  <  2:5422719/93‑1 (MQ=255)

TACCTCCTGCACGCACTGCTGACCGGTATCAGCCTGTTTGTGGCAACGCTGCTGGGTATCCACGCGGGCTTCTCTTTCTCTGCGGGGGCTATCGACTACGCGTTGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTAC  >  NC_000913/704040‑704219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`