BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	704,311	A→G	N382S (AAC→AGC)	nagE →	PTS system, N‑acetylglucosamine‑specific enzyme IIABC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	704,311	0	A	G	100.0%	715.2 / NA	203	N382S (AAC→AGC)	nagE	PTS system, N‑acetylglucosamine‑specific enzyme IIABC
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (118/85); total (118/85)

Only 100 of 215 total aligned reads displayed.

TCGTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCT  >  NC_000913/704221‑704400

tCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCa                                                                                         >  1:1315400/1‑93 (MQ=255)

tCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCa                                                                                         <  1:2020627/93‑1 (MQ=255)

  gtggtgTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCagcagc                                                                                       <  1:5683740/93‑1 (MQ=255)

   tggtgTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAgcagca                                                                                      >  1:5838517/1‑93 (MQ=255)

     gtgtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAac                                                                                    >  1:6188203/1‑93 (MQ=255)

     gtgtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAac                                                                                    <  1:2997441/93‑1 (MQ=255)

      tgtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAaca                                                                                   >  2:2376129/1‑93 (MQ=255)

       gtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAacac                                                                                  >  2:5461856/1‑93 (MQ=255)

        ttCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACt                                                                                 <  2:2119251/93‑1 (MQ=255)

          cAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTga                                                                               <  1:3800194/93‑1 (MQ=255)

           aGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTgaa                                                                              <  2:3041217/93‑1 (MQ=255)

           aGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTgaa                                                                              >  2:1782403/1‑93 (MQ=255)

           aGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTgaa                                                                              >  1:1602984/1‑93 (MQ=255)

           aGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAACATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTgaa                                                                              >  1:2292700/1‑93 (MQ=255)

               cggTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGaagaag                                                                          <  1:1880990/92‑1 (MQ=255)

               cggTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGaagaag                                                                          <  1:3914587/92‑1 (MQ=255)

                ggTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAgg                                                                         <  2:3411863/93‑1 (MQ=255)

                 gTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGTAGATAAAGAAGCCGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAAGt                                                                        >  2:2268599/1‑93 (MQ=255)

                 gTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGCCGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAggc                                                                        >  1:4610397/1‑92 (MQ=255)

                  ttATCCGCATTTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTc                                                                       <  2:4446913/93‑1 (MQ=255)

                  ttATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTc                                                                       <  2:2471283/93‑1 (MQ=255)

                  ttATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTc                                                                       <  2:5161769/93‑1 (MQ=255)

                   tATCGGAATGTACATCATAAAATCGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCt                                                                      <  1:3998297/93‑1 (MQ=255)

                    aTCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTg                                                                     >  1:6057641/1‑93 (MQ=255)

                     tCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGa                                                                    <  2:1979045/93‑1 (MQ=255)

                       cGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACt                                                                  >  2:1955283/1‑93 (MQ=255)

                       cGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACt                                                                  >  2:4886305/1‑93 (MQ=255)

                        gCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAGGAAGGTCTGACTc                                                                 >  1:2760370/1‑93 (MQ=255)

                        gCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTc                                                                 >  1:4346144/1‑93 (MQ=255)

                          aTGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCaa                                                               >  1:988876/1‑93 (MQ=255)

                           tGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAAc                                                              >  2:5228351/1‑93 (MQ=255)

                            gTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACt                                                             <  1:2087492/93‑1 (MQ=255)

                             ttCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTg                                                            >  1:5930470/1‑93 (MQ=255)

                             ttCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTg                                                            >  2:4038219/1‑93 (MQ=255)

                               cAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGc                                                          >  2:1723922/1‑93 (MQ=255)

                                aaCCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGca                                                         <  2:1848918/93‑1 (MQ=255)

                                aaCCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGca                                                         >  2:2509549/1‑93 (MQ=255)

                                 aCCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGcaa                                                        >  2:2789112/1‑93 (MQ=255)

                                   cTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGcaacc                                                      >  1:1959160/1‑93 (MQ=255)

                                     gAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGcaaccaa                                                    <  2:5395679/93‑1 (MQ=255)

                                     gAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGcaaccaa                                                    >  2:4994524/1‑93 (MQ=255)

                                       aaaCGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAAGACTGAAGAAGGTCTGACTCCACTGGCAACCAACt                                                  >  1:654077/1‑93 (MQ=255)

                                         aCGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACtat                                                <  1:1271059/93‑1 (MQ=255)

                                         aCGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACtat                                                >  2:5267118/1‑93 (MQ=255)

                                          cGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACtata                                               <  1:2376129/93‑1 (MQ=255)

                                           gCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTatat                                              <  1:2111388/93‑1 (MQ=255)

                                            ccGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATAtt                                             >  2:5804328/1‑93 (MQ=255)

                                             cGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATtg                                            >  1:2285825/1‑93 (MQ=255)

                                             cGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATtg                                            >  2:810259/1‑93 (MQ=255)

                                              gggTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATtgc                                           <  2:1923251/93‑1 (MQ=255)

                                               ggTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATtgct                                          <  2:2964791/93‑1 (MQ=255)

                                               ggTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATtgct                                          <  1:5739958/93‑1 (MQ=255)

                                                 tCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATtgctgc                                        <  2:6176245/93‑1 (MQ=255)

                                                  cGTGAAAATAAAGAAGAGGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCg                                       <  1:1398798/93‑1 (MQ=255)

                                                   gTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCgg                                      <  1:3195429/93‑1 (MQ=255)

                                                   gTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCgg                                      >  2:4421857/1‑93 (MQ=255)

                                                   gTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCgg                                      <  1:4990706/93‑1 (MQ=255)

                                                   gTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCgg                                      >  2:4556733/1‑93 (MQ=255)

                                                    tGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGt                                     >  1:4242755/1‑93 (MQ=255)

                                                    tGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGt                                     >  2:2733513/1‑93 (MQ=255)

                                                    tGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGt                                     <  1:4504702/93‑1 (MQ=255)

                                                    tGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGt                                     <  2:2856159/93‑1 (MQ=255)

                                                     gAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGtt                                    <  2:3218392/93‑1 (MQ=255)

                                                       aGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTgg                                  <  2:5901938/93‑1 (MQ=255)

                                                       aGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTgg                                  >  1:5076919/1‑93 (MQ=255)

                                                        gATAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTggg                                 >  2:1193359/1‑92 (MQ=255)

                                                          tAAAGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTggcgg                               <  2:6032009/93‑1 (MQ=255)

                                                           aaaGAAGACGAGATCGTTACTGAAGAATCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTggcggc                              <  2:3618831/93‑1 (MQ=255)

                                                           aaaGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTggcggc                              >  2:5098523/1‑93 (MQ=255)

                                                           aaaGAAGACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTggcgga                              >  2:5757430/1‑92 (MQ=255)

                                                            aagaagACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCa                             >  1:679561/1‑93 (MQ=255)

                                                            aagaagACGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCa                             >  1:4202832/1‑93 (MQ=255)

                                                             agaagaCGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCAc                            >  2:888973/1‑93 (MQ=255)

                                                             agaagaCGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCAc                            <  2:3518248/93‑1 (MQ=255)

                                                             agaagaCGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCAc                            >  1:970123/1‑93 (MQ=255)

                                                              gaagaCGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACt                           <  2:6072584/93‑1 (MQ=255)

                                                               aagaCGAGATCGTTACTGAAGAAGCCAGCATCAACACTGAAGAAGGTCGGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTg                          <  2:680193/93‑1 (MQ=255)

                                                                 gaCGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGAc                        >  2:5551702/1‑93 (MQ=255)

                                                                 gaCGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGAc                        >  1:4853782/1‑93 (MQ=255)

                                                                   cGAGATCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACaa                      >  1:220601/1‑93 (MQ=255)

                                                                     agaTCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAAcc                    >  1:356656/1‑93 (MQ=255)

                                                                      gaTCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCt                   >  1:3606041/1‑93 (MQ=255)

                                                                      gaTCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCt                   >  1:6099207/1‑93 (MQ=255)

                                                                       aTCGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTg                  >  2:4800645/1‑93 (MQ=255)

                                                                         cGTTACTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGaa                <  2:2113444/93‑1 (MQ=255)

                                                                             aCTGAAGAAGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCg            >  2:3380931/1‑93 (MQ=255)

                                                                               gtaagaagCCAGCAGCAACACTGAAGAAGGGCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGAt          <  2:5637694/91‑1 (MQ=255)

                                                                                gaagaaGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGAtt         <  2:3897345/93‑1 (MQ=255)

                                                                                gaagaaGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGAtt         >  2:3582732/1‑93 (MQ=255)

                                                                                gaagaaGCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGAtt         >  2:42659/1‑93 (MQ=255)

                                                                                 aagaagCCAGCAGCAACACTGGAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTTCGGTTGGCGGCGCTGACAATATGGAAGCGGTTg        >  1:5530023/1‑93 (MQ=255)

                                                                                  agaagCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGa       >  2:4579823/1‑93 (MQ=255)

                                                                                  agaagCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGa       >  1:2478515/1‑93 (MQ=255)

                                                                                   gaagCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGAc      >  1:3433673/1‑93 (MQ=255)

                                                                                     agCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGc    >  2:1880123/1‑93 (MQ=255)

                                                                                     agCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGc    >  2:2665184/1‑93 (MQ=255)

                                                                                      gCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGcc   <  1:533230/93‑1 (MQ=255)

                                                                                      gCCAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGcc   >  2:420923/1‑93 (MQ=255)

                                                                                       ccAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCt  <  1:5093533/93‑1 (MQ=255)

                                                                                       ccAGCAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCt  <  1:4132437/93‑1 (MQ=255)

TCGTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGGTCTGACTCAACTGGCAACCAACTATATTGCTGCGGTTGGCGGCACTGACAACCTGAAAGCGATTGACGCCT  >  NC_000913/704221‑704400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`