Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 899,581 A→T I73N (ATC→AAC)  artJ ← arginine 3rd transport system periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913899,5810AT99.1% 796.1 / ‑3.8 215I73N (ATC→AAC) artJarginine 3rd transport system periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base T (83/129);  total (85/130)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.65e-01

Only 100 of 216 total aligned reads displayed.

GCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGATCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGCACAAGGCTTTTGCCAGATCGATATCAAAGCCGACA  >  NC_000913/899492‑899670
                                                                                         |                                                                                         
 cGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAg                                                                                       <  1:378266/93‑1 (MQ=255)
 cGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAg                                                                                       <  2:2311384/93‑1 (MQ=255)
  gTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAgg                                                                                      <  1:812505/93‑1 (MQ=255)
  gTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCAAACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAgg                                                                                      >  1:5659844/1‑93 (MQ=255)
   tGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGc                                                                                     <  2:686844/93‑1 (MQ=255)
   tGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGc                                                                                     >  1:6321841/1‑93 (MQ=255)
   tGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGc                                                                                     <  1:2887774/93‑1 (MQ=255)
    ggTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCt                                                                                    >  2:5679380/1‑93 (MQ=255)
    ggTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGGTCAGGCt                                                                                    >  1:4694253/1‑93 (MQ=255)
     gTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTg                                                                                   <  2:659170/93‑1 (MQ=255)
     gTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTg                                                                                   >  2:3848642/1‑93 (MQ=255)
      tAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGt                                                                                  <  1:4328610/93‑1 (MQ=255)
       aaaCGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTc                                                                                 <  1:4025068/93‑1 (MQ=255)
       aaaCGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTc                                                                                 <  2:4355182/93‑1 (MQ=255)
       aaaCGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTc                                                                                 <  2:2508834/93‑1 (MQ=255)
          cGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGaa                                                                              <  1:1120422/93‑1 (MQ=255)
           gATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAAc                                                                             >  2:1834339/1‑93 (MQ=255)
           gATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAAc                                                                             <  1:3659214/93‑1 (MQ=255)
            aTACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAAcg                                                                            <  1:2451395/93‑1 (MQ=255)
            aTACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAAcg                                                                            >  2:4544730/1‑93 (MQ=255)
             tACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAAcgc                                                                           >  2:4261008/1‑93 (MQ=255)
              tccTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAAcgcg                                                                          <  2:4306813/92‑1 (MQ=255)
              aCCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAAcgcg                                                                          >  2:2893489/1‑93 (MQ=255)
              aCCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAAcgcg                                                                          <  1:4627011/93‑1 (MQ=255)
              aCCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTGCAGGGACGGGTTCAGGCTGTCGAAcgcg                                                                          <  1:719927/93‑1 (MQ=255)
               ccTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGt                                                                         <  1:1383619/93‑1 (MQ=255)
               ccTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGt                                                                         <  2:1359209/93‑1 (MQ=255)
                cTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTg                                                                        >  1:4231327/1‑93 (MQ=255)
                cTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTg                                                                        <  1:3438678/93‑1 (MQ=255)
                cTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTg                                                                        >  2:5964305/1‑93 (MQ=255)
                cTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTg                                                                        <  1:5538239/93‑1 (MQ=255)
                  gTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGAt                                                                      >  2:1381305/1‑93 (MQ=255)
                  gTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGAt                                                                      <  1:987852/93‑1 (MQ=255)
                    ttGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTa                                                                    <  2:2083081/93‑1 (MQ=255)
                     tGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAg                                                                   <  1:1441165/93‑1 (MQ=255)
                     tGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAg                                                                   >  1:6093565/1‑93 (MQ=255)
                     tGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAg                                                                   <  1:5375846/93‑1 (MQ=255)
                      gCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGt                                                                  >  2:3285093/1‑93 (MQ=255)
                       cTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTa                                                                 <  1:5104199/93‑1 (MQ=255)
                        tACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTaa                                                                <  1:3950879/93‑1 (MQ=255)
                         aCGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTaaa                                                               <  2:4204974/93‑1 (MQ=255)
                          cGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTaaaa                                                              >  1:4382991/1‑93 (MQ=255)
                          cGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTaaaa                                                              <  1:3568097/93‑1 (MQ=255)
                          cGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTaaaa                                                              <  1:3252050/93‑1 (MQ=255)
                             tCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTa                                                           <  2:1502015/93‑1 (MQ=255)
                             tCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTa                                                           <  2:5732943/93‑1 (MQ=255)
                              ccGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTAc                                                          <  1:6070847/93‑1 (MQ=255)
                              ccGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTAc                                                          >  2:4692455/1‑93 (MQ=255)
                                 gggTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACAtt                                                       >  2:2343538/1‑93 (MQ=255)
                                  ggTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTc                                                      <  1:5645983/93‑1 (MQ=255)
                                  ggTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTc                                                      <  2:5590134/93‑1 (MQ=255)
                                  ggTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTc                                                      <  2:2810266/93‑1 (MQ=255)
                                  ggTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTc                                                      <  1:2243399/93‑1 (MQ=255)
                                    tGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTctg                                                    <  1:2144866/93‑1 (MQ=255)
                                     gATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTctgc                                                   >  2:100210/1‑93 (MQ=255)
                                      atatCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTctgcc                                                  <  1:1548249/93‑1 (MQ=255)
                                       tatCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTctgcct                                                 <  2:5928170/93‑1 (MQ=255)
                                       tatCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTctgcct                                                 >  2:2702828/1‑93 (MQ=255)
                                          ccATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCa                                              <  1:5824358/93‑1 (MQ=255)
                                           cATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCAt                                             >  1:1636199/1‑93 (MQ=255)
                                            aTACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCAtt                                            <  1:6427017/93‑1 (MQ=255)
                                            aTACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCAtt                                            <  1:5069593/93‑1 (MQ=255)
                                            aTACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCAtt                                            >  2:2231157/1‑93 (MQ=255)
                                             tACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCAttt                                           >  2:6062188/1‑93 (MQ=255)
                                               ccGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCAtttgt                                         >  2:1257454/1‑93 (MQ=255)
                                                cGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCAtttgtt                                        <  1:4855540/93‑1 (MQ=255)
                                                cGGAGAGTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCAtttgtt                                        <  2:6358106/93‑1 (MQ=255)
                                                 ggAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCAtttgttt                                       <  1:3164927/93‑1 (MQ=255)
                                                  gagaTTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCAtttgtttg                                      >  1:543749/1‑93 (MQ=255)
                                                   agaTTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGc                                     >  2:6412873/1‑93 (MQ=255)
                                                    gaTTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGca                                    >  2:1925789/1‑93 (MQ=255)
                                                    gaTTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGca                                    >  2:2761362/1‑93 (MQ=255)
                                                     aTTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGcac                                   <  2:4702728/93‑1 (MQ=255)
                                                     aTTACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGcac                                   <  1:4612339/93‑1 (MQ=255)
                                                       tACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGCACaa                                 >  2:2174760/1‑93 (MQ=255)
                                                       tACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGCACaa                                 <  2:3110806/93‑1 (MQ=255)
                                                       tACGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGCACaa                                 >  2:3786370/1‑93 (MQ=255)
                                                        aCGGCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGCACAAg                                <  1:2761658/93‑1 (MQ=255)
                                                          ggCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAATGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGCACAAGGc                              <  2:263894/93‑1 (MQ=255)
                                                           gCGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGCACAAGGCt                             <  2:771577/93‑1 (MQ=255)
                                                            cGTCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGCACAAGGCtt                            <  1:2419391/93‑1 (MQ=255)
                                                              tCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGCACAAGGCtttt                          <  1:2521561/93‑1 (MQ=255)
                                                              tCATATTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGCACAAGGCtttt                          >  2:5235238/1‑93 (MQ=255)
                                                                atatTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGCACAAGGCTTTTGc                        <  1:3589206/93‑1 (MQ=255)
                                                                atatTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGCACAAGGCTTTTGc                        <  1:124380/93‑1 (MQ=255)
                                                                atatTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGCACAAGGCTTTTGc                        <  2:3534111/93‑1 (MQ=255)
                                                                  atTTTCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGCACAAGGCTTTTGCCa                      <  2:4410713/93‑1 (MQ=255)
                                                                   ttttCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGCACAAGGCTTTTGCCAg                     <  1:1709620/93‑1 (MQ=255)
                                                                     ttCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGCACAAGGCTTTTGCCAGAt                   <  1:5977659/93‑1 (MQ=255)
                                                                     ttCTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGCACAAGGCTTTTGCCAGAt                   >  2:3871845/1‑93 (MQ=255)
                                                                       cTGAATTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGCACAAGGCTTTTGCCAGATCg                 <  2:2333179/93‑1 (MQ=255)
                                                                           aTTTCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGCACAAGGCTTTTGCCAGATCGatat             >  1:1034350/1‑93 (MQ=255)
                                                                             ttCAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGCACAAGGCTTTTGCCAGATCGATATCa           >  1:2085674/1‑93 (MQ=255)
                                                                               cAGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGCACAAGGCTTTTGCCAGATCGATATCaaa         >  1:5596517/1‑93 (MQ=255)
                                                                                aGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGCACAAGGCTTTTGCCAGATCGATATCAAAg        >  2:3370525/1‑93 (MQ=255)
                                                                                aGGGACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGCACAAGGCTTTTGCCAGATCGATATCAAAg        >  2:6327432/1‑93 (MQ=255)
                                                                                 gggACGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGCACAAGGCTTTTGCCAGATCGATATCAAAGc       <  1:4399228/93‑1 (MQ=255)
                                                                                  ggACGGGATCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGCACAAGGCTTTTGCCAGATCGATATCAAAGcc      >  1:5211687/1‑93 (MQ=255)
                                                                                     cGGGTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGCACAAGGCTTTTGCCAGATCGATATCAAAGCCGAc   <  1:3535388/93‑1 (MQ=255)
                                                                                      gggTTCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGCACAAGGCTTTTGCCAGATCGATATCAAAGCCGACa  >  1:3101798/1‑93 (MQ=255)
                                                                                         |                                                                                         
GCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGGGATCAGGCTGTCGAACGCGTGATTAGTAAAAGTACATTCTGCCTGCATTTGTTTGCACAAGGCTTTTGCCAGATCGATATCAAAGCCGACA  >  NC_000913/899492‑899670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑